Rroxscaffold_2G00147560
ERF Family

Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008043
Physical Location & Seq
Forward (+)
85353755 .. 85355407
1653 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00147560.1

Sequence Viewer

Length: 594 bp
ATGAGCATCAGCTCTTCCATGGCTTCCCTTCTCACCCCAACCCCAACCATTACACTCCAACACAAGAAACATCATTTCAGGCTCAACACAAACCCACCATCCAGAATTTCCAAGAGAAATTTTGGGCGCCATGGGCTTTGTTTTGTGACAAGAGCTAAGGTTGGAGGAGATGTACTTGAGAAACATTCAGTTAGTGCTGAAGACAAGAAGGATTATGGACTAGTTAGTCTCCACCATGTTGGAATCTTGTGTGAAAACTTGGAGAGGTCGCTCGACTTTTATCAGAACATTCTTGGTCTTGAAATAAATGAGGCTAGGCCAGATGACAAACTCCCCTATAGGGGGGCTTGGTTATGGGTGGGTTCTGAGATGATTCACCTTATGGAGCTTCCAAACCCCGACCCTCTAACCGGAAGACCGGAGCATGGAGGCCGTGATAGGCACACTTGTGTTGAGATTCGGGATGTGTCTAAGCTGAAAGCAATCCTTGATGAAGCTGGTATTCCATACACGCTTAGTAAGTCTGGGAGGCCAGCAATCTTTTTCGAGATCCAGATGAAAATGCTCTGGAATTTGCACAGTTCTGATGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

197

Amino Acids

22.23

Weight (kDa)

8.44

Isoelectric Point (pI)

38.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 77 - 169 8.6e-10 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 78 - 172 8e-07 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 225
AccB1I GGYRCC 1 cut(s) 126
AclWI GGATC 1 cut(s) 544
AcsI RAATTY 3 cut(s) 105, 118, 571
AcuI CTGAAG 1 cut(s) 219
AcyI GRCGYC 1 cut(s) 127
AfaI GTAC 1 cut(s) 174
AfiI CCNNNNNNNGG 5 cut(s) 340, 341, 342, 410, 425
AgsI TTSAA 1 cut(s) 302
AhlI ACTAGT 1 cut(s) 220
AleI CACNNNNGTG 1 cut(s) 447
AluBI AGCT 5 cut(s) 12, 155, 388, 475, 497
AluI AGCT 5 cut(s) 12, 155, 388, 475, 497
Alw26I GTCTC 1 cut(s) 233
AlwI GGATC 1 cut(s) 544
AoxI GGCC 3 cut(s) 317, 430, 530
ApoI RAATTY 3 cut(s) 105, 118, 571
AspLEI GCGC 1 cut(s) 129
AsuHPI GGTGA 2 cut(s) 25, 368
BanI GGYRCC 1 cut(s) 126
BbsI GAAGAC 2 cut(s) 207, 421
BccI CCATC 2 cut(s) 106, 581
BceAI ACGGC 1 cut(s) 417
BcoDI GTCTC 1 cut(s) 233
BcuI ACTAGT 1 cut(s) 220
BfaI CTAG 2 cut(s) 221, 315
BfmI CTRYAG 1 cut(s) 337
BfoI RGCGCY 1 cut(s) 130
BmiI GGNNCC 1 cut(s) 128
BmsI GCATC 1 cut(s) 15
BpiI GAAGAC 2 cut(s) 207, 421
Bpu10I CCTNAGC 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 197
BsaHI GRCGYC 1 cut(s) 127
BsaJI CCNNGG 2 cut(s) 18, 130
BsaWI WCCGGW 2 cut(s) 410, 418
BsaXI ACNNNNNCTCC 2 cut(s) 156, 186
Bsc4I CCNNNNNNNGG 5 cut(s) 340, 341, 342, 410, 425
BseDI CCNNGG 2 cut(s) 18, 130
BseGI GGATG 2 cut(s) 98, 469
BseLI CCNNNNNNNGG 5 cut(s) 340, 341, 342, 410, 425
BseMII CTCAG 1 cut(s) 357
BseRI GAGGAG 1 cut(s) 180
BshFI GGCC 3 cut(s) 319, 432, 532
BshNI GGYRCC 1 cut(s) 126
BsiSI CCGG 2 cut(s) 411, 419
BslI CCNNNNNNNGG 5 cut(s) 340, 341, 342, 410, 425
BsmAI GTCTC 1 cut(s) 233
BsnI GGCC 3 cut(s) 319, 432, 532
Bsp143I GATC 1 cut(s) 549
Bsp19I CCATGG 2 cut(s) 18, 130
BspANI GGCC 3 cut(s) 319, 432, 532
BspCNI CTCAG 1 cut(s) 358
BspLI GGNNCC 1 cut(s) 128
BspPI GGATC 1 cut(s) 544
BspQI GCTCTTC 1 cut(s) 19
BspT107I GGYRCC 1 cut(s) 126
BssECI CCNNGG 2 cut(s) 18, 130
BssMI GATC 1 cut(s) 549
BssNI GRCGYC 1 cut(s) 127
BssT1I CCWWGG 2 cut(s) 18, 130
Bst4CI ACNGT 1 cut(s) 581
Bst6I CTCTTC 1 cut(s) 19
BstACI GRCGYC 1 cut(s) 127
BstC8I GCNNGC 1 cut(s) 534
BstDEI CTNAG 4 cut(s) 156, 366, 471, 515
BstDSI CCRYGG 2 cut(s) 18, 130
BstF5I GGATG 2 cut(s) 98, 469
BstH2I RGCGCY 1 cut(s) 130
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 1 cut(s) 552
BstMAI GTCTC 1 cut(s) 233
BstMBI GATC 1 cut(s) 549
BstMWI GCNNNNNNNGC 1 cut(s) 133
BstSFI CTRYAG 1 cut(s) 337
BstV2I GAAGAC 2 cut(s) 207, 421
BstX2I RGATCY 1 cut(s) 549
BstXI CCANNNNNNTGG 1 cut(s) 239
BstYI RGATCY 1 cut(s) 549
BsuRI GGCC 3 cut(s) 319, 432, 532
BtgI CCRYGG 2 cut(s) 18, 130
BtsCI GGATG 2 cut(s) 98, 469
Cac8I GCNNGC 1 cut(s) 534
CfoI GCGC 1 cut(s) 129
Csp6I GTAC 1 cut(s) 173
CviAII CATG 4 cut(s) 19, 131, 236, 425
CviQI GTAC 1 cut(s) 173
DdeI CTNAG 4 cut(s) 156, 366, 471, 515
DinI GGCGCC 1 cut(s) 128
DpnI GATC 1 cut(s) 551
DpnII GATC 1 cut(s) 549
DrdI GACNNNNNNGTC 1 cut(s) 225
DseDI GACNNNNNNGTC 1 cut(s) 225
Eam1104I CTCTTC 1 cut(s) 19
EarI CTCTTC 1 cut(s) 19
Eco130I CCWWGG 2 cut(s) 18, 130
Eco57I CTGAAG 1 cut(s) 219
EcoT14I CCWWGG 2 cut(s) 18, 130
EgeI GGCGCC 1 cut(s) 128
EheI GGCGCC 1 cut(s) 128
ErhI CCWWGG 2 cut(s) 18, 130
FaeI CATG 4 cut(s) 22, 134, 239, 428
FaiI YATR 9 cut(s) 20, 132, 216, 237, 339, 355, 383, 426, 508
FalI AAGNNNNNCTT 2 cut(s) 471, 503
FatI CATG 4 cut(s) 18, 130, 235, 424
FokI GGATG 2 cut(s) 85, 476
FspBI CTAG 2 cut(s) 221, 315
GlaI GCGC 1 cut(s) 128
HaeII RGCGCY 1 cut(s) 130
HaeIII GGCC 3 cut(s) 319, 432, 532
HapII CCGG 2 cut(s) 411, 419
HhaI GCGC 1 cut(s) 129
Hin1I GRCGYC 1 cut(s) 127
Hin1II CATG 4 cut(s) 22, 134, 239, 428
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HinfI GANTC 3 cut(s) 243, 373, 457
HpaII CCGG 2 cut(s) 411, 419
HphI GGTGA 2 cut(s) 25, 368
Hpy188I TCNGA 3 cut(s) 285, 367, 586
Hpy188III TCNNGA 6 cut(s) 102, 299, 461, 547, 553, 568
HpyAV CCTTC 2 cut(s) 38, 202
HpyCH4III ACNGT 1 cut(s) 581
HpyCH4V TGCA 1 cut(s) 577
HpyF10VI GCNNNNNNNGC 1 cut(s) 133
HpyF3I CTNAG 4 cut(s) 156, 366, 471, 515
Hsp92I GRCGYC 1 cut(s) 127
Hsp92II CATG 4 cut(s) 22, 134, 239, 428
HspAI GCGC 1 cut(s) 127
KasI GGCGCC 1 cut(s) 126
Kzo9I GATC 1 cut(s) 549
LguI GCTCTTC 1 cut(s) 19
LmnI GCTCC 2 cut(s) 385, 421
LweI GCATC 1 cut(s) 15
MaeI CTAG 2 cut(s) 221, 315
MaeIII GTNAC 1 cut(s) 145
MalI GATC 1 cut(s) 551
MboI GATC 1 cut(s) 549
MboII GAAGA 3 cut(s) 6, 212, 426
MflI RGATCY 1 cut(s) 549
MluCI AATT 3 cut(s) 105, 118, 571
Mly113I GGCGCC 1 cut(s) 127
MmeI TCCRAC 3 cut(s) 82, 142, 220
MnlI CCTC 6 cut(s) 158, 258, 304, 414, 422, 522
MslI CAYNNNNRTG 1 cut(s) 447
MspI CCGG 2 cut(s) 411, 419
MwoI GCNNNNNNNGC 1 cut(s) 133
NarI GGCGCC 1 cut(s) 127
NcoI CCATGG 2 cut(s) 18, 130
NdeII GATC 1 cut(s) 549
NlaIII CATG 4 cut(s) 22, 134, 239, 428
NlaIV GGNNCC 1 cut(s) 128
NmuCI GTSAC 1 cut(s) 145
OliI CACNNNNGTG 1 cut(s) 447
PciSI GCTCTTC 1 cut(s) 19
PfeI GAWTC 3 cut(s) 243, 373, 457
PluTI GGCGCC 1 cut(s) 130
PspN4I GGNNCC 1 cut(s) 128
PsuI RGATCY 1 cut(s) 549
RsaI GTAC 1 cut(s) 174
RsaNI GTAC 1 cut(s) 173
RseI CAYNNNNRTG 1 cut(s) 447
SapI GCTCTTC 1 cut(s) 19
Sau3AI GATC 1 cut(s) 549
SetI ASST 8 cut(s) 14, 157, 162, 269, 381, 390, 477, 499
SfaNI GCATC 1 cut(s) 15
SfcI CTRYAG 1 cut(s) 337
SfoI GGCGCC 1 cut(s) 128
SmiMI CAYNNNNRTG 1 cut(s) 447
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
SpeI ACTAGT 1 cut(s) 220
Sse9I AATT 3 cut(s) 105, 118, 571
SspDI GGCGCC 1 cut(s) 126
SspMI CTAG 2 cut(s) 221, 315
StyI CCWWGG 2 cut(s) 18, 130
TaaI ACNGT 1 cut(s) 581
TaqI TCGA 2 cut(s) 273, 546
TasI AATT 3 cut(s) 105, 118, 571
TatI WGTACW 1 cut(s) 172
TfiI GAWTC 3 cut(s) 243, 373, 457
TseFI GTSAC 1 cut(s) 145
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 2 cut(s) 507, 572
XapI RAATTY 3 cut(s) 105, 118, 571
XspI CTAG 2 cut(s) 221, 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.