MD02G1084200.v1.1
ERF Family

Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008043
Physical Location & Seq
Reverse (-)
6603412 .. 6605538
2127 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1084200.v1.1.491

Sequence Viewer

Length: 732 bp
ATGAGCAGCTGCTCTTCCATGGCTTCCCTTCTCATCCCACCACCCACTCTTCGTCTCCAACTCAAGGAGGATGGGATCAGAGTCAGCACTGTGCTTACGAACTTATCATCTAAAATTGACCGGACCTGTCTGAGAAGTTGCAGACGTCAGGGACGGTGTTTTGTGACGAAAGCTCAGGTCACCGTTGAAGAAATTGTACTGGAGAAGCAATCAGTTGGTGATCAGGACCAAAAAGATTTTGGAGTTGTTGGTGTCCACCATGTCGGATTGTTGTGTGAAAACCTCGAAAGATCTCTCAGCTTTTACCAAAACCTTTTAGGTCTTGAAATAAATGAGGCCAGGCCACATGACAAGCTCCCCTTCAGAGGTGCCTGGTTATGGGTTGGTTCAGAGATGATTCATCTCATGGAACTTCCAAATCCCGACCCTTTAACCGGAAGACCAGCCCATGGAGGTCGTGACCGCCATACTTGTATTGCAATCCGGGATGTGTCTAAGCTCAAAGCAATCCTCGACGACGCTGGCAAGCTTTTCTTTTCCTCATCTGCAGTTCATTTCATTTTCGATAACATTTGGAACGAATGTTTGATCTATGAAAGGAATGTGGACGGCTTACATGGCTGTTTTTGGCTGCAGGTATTACCTACACGCCTAGCAAGTCCGGGAGGCCTGCGATTTTTACACGAGACCCGGACGCAAATGCTCTGGAGTTTACTCAAGTTTGATAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

244

Amino Acids

27.45

Weight (kDa)

7.64

Isoelectric Point (pI)

49.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 85 - 175 5e-08 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 148
Acc36I ACCTGC 1 cut(s) 625
AccB1I GGYRCC 1 cut(s) 368
AccB7I CCANNNNNTGG 1 cut(s) 449
AciI CCGC 1 cut(s) 463
AclWI GGATC 1 cut(s) 83
AcuI CTGAAG 1 cut(s) 346
AcyI GRCGYC 1 cut(s) 145
AfaI GTAC 1 cut(s) 198
AfiI CCNNNNNNNGG 5 cut(s) 64, 365, 378, 434, 449
AgsI TTSAA 2 cut(s) 188, 326
AjnI CCWGG 2 cut(s) 338, 371
AluBI AGCT 7 cut(s) 9, 173, 300, 355, 499, 529, 729
AluI AGCT 7 cut(s) 9, 173, 300, 355, 499, 529, 729
Alw26I GTCTC 2 cut(s) 59, 680
AlwI GGATC 1 cut(s) 83
AoxI GGCC 3 cut(s) 336, 341, 667
ApeKI GCWGC 3 cut(s) 6, 9, 631
AspS9I GGNCC 2 cut(s) 123, 226
AsuC2I CCSGG 3 cut(s) 485, 663, 691
AsuHPI GGTGA 2 cut(s) 172, 230
AvaII GGWCC 2 cut(s) 123, 226
BanI GGYRCC 1 cut(s) 368
BarI GAAGNNNNNNTAC 2 cut(s) 180, 212
BauI CACGAG 1 cut(s) 683
BbsI GAAGAC 1 cut(s) 445
BbvI GCAGC 2 cut(s) 18, 618
BccI CCATC 1 cut(s) 65
BceAI ACGGC 1 cut(s) 625
BciT130I CCWGG 2 cut(s) 340, 373
BclI TGATCA 1 cut(s) 220
BcnI CCSGG 3 cut(s) 485, 663, 691
BcoDI GTCTC 2 cut(s) 59, 680
BfaI CTAG 1 cut(s) 653
BfmI CTRYAG 2 cut(s) 546, 632
BfuAI ACCTGC 1 cut(s) 625
BglII AGATCT 1 cut(s) 290
BisI GCNGC 3 cut(s) 7, 10, 632
BlsI GCNGC 3 cut(s) 8, 11, 633
Bme1390I CCNGG 5 cut(s) 340, 373, 485, 663, 691
Bme18I GGWCC 2 cut(s) 123, 226
BmgT120I GGNCC 2 cut(s) 123, 226
BmiI GGNNCC 1 cut(s) 370
BmrFI CCNGG 5 cut(s) 340, 373, 485, 663, 691
BpiI GAAGAC 1 cut(s) 445
BpmI CTGGAG 2 cut(s) 221, 727
Bpu10I CCTNAGC 1 cut(s) 174
BpuEI CTTGAG 2 cut(s) 47, 701
BpuMI CCSGG 3 cut(s) 485, 663, 691
BsaHI GRCGYC 1 cut(s) 145
BsaI GGTCTC 1 cut(s) 680
BsaJI CCNNGG 2 cut(s) 18, 448
BsaWI WCCGGW 2 cut(s) 120, 434
Bsc4I CCNNNNNNNGG 5 cut(s) 64, 365, 378, 434, 449
Bse1I ACTGG 1 cut(s) 204
BseBI CCWGG 2 cut(s) 340, 373
BseDI CCNNGG 2 cut(s) 18, 448
BseGI GGATG 3 cut(s) 33, 76, 493
BseLI CCNNNNNNNGG 5 cut(s) 64, 365, 378, 434, 449
BseMII CTCAG 3 cut(s) 122, 188, 310
BseNI ACTGG 1 cut(s) 204
BseXI GCAGC 2 cut(s) 18, 618
BshFI GGCC 3 cut(s) 338, 343, 669
BshNI GGYRCC 1 cut(s) 368
BsiSI CCGG 5 cut(s) 121, 435, 484, 662, 691
BslFI GGGAC 1 cut(s) 165
BslI CCNNNNNNNGG 5 cut(s) 64, 365, 378, 434, 449
BsmAI GTCTC 2 cut(s) 59, 680
BsmBI CGTCTC 1 cut(s) 59
BsmFI GGGAC 1 cut(s) 165
BsnI GGCC 3 cut(s) 338, 343, 669
Bso31I GGTCTC 1 cut(s) 680
Bsp143I GATC 4 cut(s) 75, 220, 290, 588
Bsp19I CCATGG 2 cut(s) 18, 448
BspACI CCGC 1 cut(s) 463
BspANI GGCC 3 cut(s) 338, 343, 669
BspCNI CTCAG 3 cut(s) 123, 187, 309
BspLI GGNNCC 1 cut(s) 370
BspMAI CTGCAG 2 cut(s) 550, 636
BspMI ACCTGC 1 cut(s) 625
BspPI GGATC 1 cut(s) 83
BspQI GCTCTTC 1 cut(s) 19
BspT107I GGYRCC 1 cut(s) 368
BspTNI GGTCTC 1 cut(s) 680
BsrI ACTGG 1 cut(s) 204
BssECI CCNNGG 2 cut(s) 18, 448
BssMI GATC 4 cut(s) 75, 220, 290, 588
BssNI GRCGYC 1 cut(s) 145
BssSI CACGAG 1 cut(s) 683
BssT1I CCWWGG 2 cut(s) 18, 448
Bst2BI CACGAG 1 cut(s) 683
Bst2UI CCWGG 2 cut(s) 340, 373
Bst4CI ACNGT 3 cut(s) 91, 156, 184
Bst6I CTCTTC 2 cut(s) 19, 54
BstACI GRCGYC 1 cut(s) 145
BstC8I GCNNGC 3 cut(s) 523, 527, 671
BstDEI CTNAG 4 cut(s) 131, 174, 296, 495
BstDSI CCRYGG 2 cut(s) 18, 448
BstEII GGTNACC 1 cut(s) 178
BstF5I GGATG 3 cut(s) 33, 76, 493
BstKTI GATC 4 cut(s) 78, 223, 293, 591
BstMAI GTCTC 2 cut(s) 59, 680
BstMBI GATC 4 cut(s) 75, 220, 290, 588
BstMWI GCNNNNNNNGC 1 cut(s) 618
BstNI CCWGG 2 cut(s) 340, 373
BstPI GGTNACC 1 cut(s) 178
BstSCI CCNGG 5 cut(s) 338, 371, 483, 661, 689
BstSFI CTRYAG 2 cut(s) 546, 632
BstV1I GCAGC 2 cut(s) 18, 618
BstV2I GAAGAC 1 cut(s) 445
BstX2I RGATCY 1 cut(s) 290
BstYI RGATCY 1 cut(s) 290
BsuRI GGCC 3 cut(s) 338, 343, 669
BtgI CCRYGG 2 cut(s) 18, 448
BtsCI GGATG 3 cut(s) 33, 76, 493
BtsIMutI CAGTG 1 cut(s) 87
BveI ACCTGC 1 cut(s) 625
Cac8I GCNNGC 3 cut(s) 523, 527, 671
Cfr13I GGNCC 2 cut(s) 123, 226
CseI GACGC 2 cut(s) 527, 703
Csp6I GTAC 1 cut(s) 197
CviAII CATG 6 cut(s) 19, 260, 347, 406, 449, 617
CviQI GTAC 1 cut(s) 197
DdeI CTNAG 4 cut(s) 131, 174, 296, 495
DpnI GATC 4 cut(s) 77, 222, 292, 590
DpnII GATC 4 cut(s) 75, 220, 290, 588
Eam1104I CTCTTC 2 cut(s) 19, 54
EarI CTCTTC 2 cut(s) 19, 54
Eco130I CCWWGG 2 cut(s) 18, 448
Eco147I AGGCCT 1 cut(s) 669
Eco31I GGTCTC 1 cut(s) 680
Eco47I GGWCC 2 cut(s) 123, 226
Eco57I CTGAAG 1 cut(s) 346
Eco91I GGTNACC 1 cut(s) 178
EcoO65I GGTNACC 1 cut(s) 178
EcoRII CCWGG 2 cut(s) 338, 371
EcoT14I CCWWGG 2 cut(s) 18, 448
ErhI CCWWGG 2 cut(s) 18, 448
Esp3I CGTCTC 1 cut(s) 59
FaeI CATG 6 cut(s) 22, 263, 350, 409, 452, 620
FaiI YATR 9 cut(s) 20, 261, 348, 379, 407, 450, 468, 594, 618
FalI AAGNNNNNCTT 4 cut(s) 344, 376, 518, 550
FaqI GGGAC 1 cut(s) 165
FatI CATG 6 cut(s) 18, 259, 346, 405, 448, 616
FbaI TGATCA 1 cut(s) 220
Fnu4HI GCNGC 3 cut(s) 7, 10, 632
FokI GGATG 3 cut(s) 20, 83, 500
Fsp4HI GCNGC 3 cut(s) 7, 10, 632
FspBI CTAG 1 cut(s) 653
GluI GCNGC 3 cut(s) 7, 10, 632
GsuI CTGGAG 2 cut(s) 221, 727
HaeIII GGCC 3 cut(s) 338, 343, 669
HapII CCGG 5 cut(s) 121, 435, 484, 662, 691
HgaI GACGC 2 cut(s) 527, 703
Hin1I GRCGYC 1 cut(s) 145
Hin1II CATG 6 cut(s) 22, 263, 350, 409, 452, 620
HindIII AAGCTT 1 cut(s) 527
HinfI GANTC 2 cut(s) 81, 397
HpaII CCGG 5 cut(s) 121, 435, 484, 662, 691
HphI GGTGA 2 cut(s) 172, 230
Hpy166II GTNNAC 3 cut(s) 256, 607, 713
Hpy188I TCNGA 5 cut(s) 80, 132, 266, 365, 391
Hpy188III TCNNGA 5 cut(s) 224, 323, 422, 458, 706
Hpy8I GTNNAC 3 cut(s) 256, 607, 713
Hpy99I CGWCG 2 cut(s) 518, 521
HpyAV CCTTC 2 cut(s) 38, 370
HpyCH4III ACNGT 3 cut(s) 91, 156, 184
HpyCH4IV ACGT 1 cut(s) 145
HpyCH4V TGCA 4 cut(s) 141, 479, 548, 634
HpyF10VI GCNNNNNNNGC 1 cut(s) 618
HpyF3I CTNAG 4 cut(s) 131, 174, 296, 495
HpySE526I ACGT 1 cut(s) 145
Hsp92I GRCGYC 1 cut(s) 145
Hsp92II CATG 6 cut(s) 22, 263, 350, 409, 452, 620
Ksp22I TGATCA 1 cut(s) 220
Kzo9I GATC 4 cut(s) 75, 220, 290, 588
LguI GCTCTTC 1 cut(s) 19
LmnI GCTCC 1 cut(s) 360
Lsp1109I GCAGC 2 cut(s) 18, 618
MaeI CTAG 1 cut(s) 653
MaeII ACGT 1 cut(s) 145
MaeIII GTNAC 3 cut(s) 163, 178, 458
MalI GATC 4 cut(s) 77, 222, 292, 590
MboI GATC 4 cut(s) 75, 220, 290, 588
MboII GAAGA 4 cut(s) 6, 41, 200, 450
MflI RGATCY 1 cut(s) 290
MluCI AATT 2 cut(s) 114, 192
MlyI GAGTC 1 cut(s) 90
MmeI TCCRAC 2 cut(s) 82, 244
MnlI CCTC 8 cut(s) 61, 293, 328, 359, 446, 521, 550, 659
MseI TTAA 1 cut(s) 431
MspA1I CMGCKG 1 cut(s) 9
MspI CCGG 5 cut(s) 121, 435, 484, 662, 691
MspR9I CCNGG 5 cut(s) 340, 373, 485, 663, 691
MvaI CCWGG 2 cut(s) 340, 373
MwoI GCNNNNNNNGC 1 cut(s) 618
NciI CCSGG 3 cut(s) 485, 663, 691
NcoI CCATGG 2 cut(s) 18, 448
NdeII GATC 4 cut(s) 75, 220, 290, 588
NlaIII CATG 6 cut(s) 22, 263, 350, 409, 452, 620
NlaIV GGNNCC 1 cut(s) 370
NmuCI GTSAC 3 cut(s) 163, 178, 458
PceI AGGCCT 1 cut(s) 669
PciSI GCTCTTC 1 cut(s) 19
PfeI GAWTC 1 cut(s) 397
PflMI CCANNNNNTGG 1 cut(s) 449
PfoI TCCNGGA 2 cut(s) 483, 661
PkrI GCNGC 3 cut(s) 8, 11, 633
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
Psp6I CCWGG 2 cut(s) 338, 371
PspEI GGTNACC 1 cut(s) 178
PspGI CCWGG 2 cut(s) 338, 371
PspN4I GGNNCC 1 cut(s) 370
PspPI GGNCC 2 cut(s) 123, 226
PstI CTGCAG 2 cut(s) 550, 636
PsuI RGATCY 1 cut(s) 290
PvuII CAGCTG 1 cut(s) 9
RsaI GTAC 1 cut(s) 198
RsaNI GTAC 1 cut(s) 197
SapI GCTCTTC 1 cut(s) 19
SaqAI TTAA 1 cut(s) 431
SatI GCNGC 3 cut(s) 7, 10, 632
Sau3AI GATC 4 cut(s) 75, 220, 290, 588
Sau96I GGNCC 2 cut(s) 123, 226
SchI GAGTC 1 cut(s) 90
ScrFI CCNGG 5 cut(s) 340, 373, 485, 663, 691
SfcI CTRYAG 2 cut(s) 546, 632
SinI GGWCC 2 cut(s) 123, 226
SmlI CTYRAG 2 cut(s) 62, 716
SmoI CTYRAG 2 cut(s) 62, 716
Sse9I AATT 2 cut(s) 114, 192
SseBI AGGCCT 1 cut(s) 669
SsiI CCGC 1 cut(s) 463
SspMI CTAG 1 cut(s) 653
StuI AGGCCT 1 cut(s) 669
StyD4I CCNGG 5 cut(s) 338, 371, 483, 661, 689
StyI CCWWGG 2 cut(s) 18, 448
TaaI ACNGT 3 cut(s) 91, 156, 184
TaiI ACGT 1 cut(s) 148
TaqI TCGA 3 cut(s) 285, 513, 564
TasI AATT 2 cut(s) 114, 192
TatI WGTACW 1 cut(s) 196
TfiI GAWTC 1 cut(s) 397
Tru1I TTAA 1 cut(s) 431
Tru9I TTAA 1 cut(s) 431
TscAI CASTG 1 cut(s) 94
TseFI GTSAC 3 cut(s) 163, 178, 458
TseI GCWGC 3 cut(s) 6, 9, 631
Tsp45I GTSAC 3 cut(s) 163, 178, 458
TspDTI ATGAA 4 cut(s) 389, 542, 547, 609
TspRI CASTG 1 cut(s) 94
Van91I CCANNNNNTGG 1 cut(s) 449
VpaK11BI GGWCC 2 cut(s) 123, 226
XcmI CCANNNNNNNNNTGG 1 cut(s) 236
XspI CTAG 1 cut(s) 653
ZraI GACGTC 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.