RLG00000016445
ERF Family

Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008043
Physical Location & Seq
Reverse (-)
6690506 .. 6691808
1303 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016445

Sequence Viewer

Length: 777 bp
ATGAGCAGCAGCTCTTCCATGGCTTCCCTTCTCACCCCAACCCCAACCCCAACCCCAACCATGACACTCCAACACAAGAAAGATCATTTCAGGCTCAACACAAACCCACCAGCCAGAATTTCCAAGAGAAATTTTGGACGCCATGGGCTTTGTTTTGTGACAAGAGCTAAGGTTGGAGGAGATGTACTTGAGAAACAATCAGTTAGTGCTGAAGACAAGAAGGATTATGGCCTGGTTAGTCTCCACCATGTTGGAATCTTGTGTGAAAACTTGGAGAGGTCGCTCGACTTTTATCAGAACATTCTTGGTCTTGAAATAAATGAGGCTAGGCCAGATGACAAACTCCCCTATAGGGGGGCTTGGTTATGGGTGGGTTCTGAGATGATTCACCTTATGGAGCTTCCAAACCCCGACCCTCTAACCGGAAGGCCGGAGCATGGAGGCCGTGATAGGCACACTTGTGTTGAGATTCGGGATGTGTCTAAGCTGAAAGCAATCCTTGATGAAGCTGGCAAGCTTTTATTTCCTCATTTGAAGTTCATTTCATATGTGAATGATTTTGGAAGGAATGCTGATTGGTTTACTTTGCAATTATCTATACTGCAGGTATTCCATACACGCTTAGTAAGTCTGGGAGGCCAGCAATCTTTTTTCGAGATCCAGATGAAAATGCTCTGGAATTTGCACAGTTCTGATGGCTTACATGTTGCAGCACCCGTAGTGTTACATTGTGATATTGATAATGCCTTCAGGGGTTCCATTATGTTCAGGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

259

Amino Acids

29.2

Weight (kDa)

7.8

Isoelectric Point (pI)

42.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 81 - 170 3.7e-09 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 595
AclWI GGATC 1 cut(s) 652
AcsI RAATTY 3 cut(s) 117, 130, 679
AcuI CTGAAG 2 cut(s) 231, 733
AcyI GRCGYC 1 cut(s) 139
AfaI GTAC 1 cut(s) 186
AfiI CCNNNNNNNGG 5 cut(s) 352, 353, 354, 422, 437
AflIII ACRYGT 1 cut(s) 703
AgsI TTSAA 2 cut(s) 314, 535
AjnI CCWGG 1 cut(s) 231
AleI CACNNNNGTG 1 cut(s) 459
AluBI AGCT 6 cut(s) 12, 167, 400, 487, 509, 517
AluI AGCT 6 cut(s) 12, 167, 400, 487, 509, 517
Alw26I GTCTC 1 cut(s) 245
AlwI GGATC 1 cut(s) 652
AoxI GGCC 5 cut(s) 229, 329, 428, 442, 637
ApeKI GCWGC 3 cut(s) 6, 9, 710
ApoI RAATTY 3 cut(s) 117, 130, 679
AsuHPI GGTGA 2 cut(s) 25, 380
BbsI GAAGAC 1 cut(s) 219
BbvI GCAGC 3 cut(s) 18, 21, 722
BccI CCATC 1 cut(s) 689
BceAI ACGGC 1 cut(s) 429
BciT130I CCWGG 1 cut(s) 233
BcoDI GTCTC 1 cut(s) 245
BfaI CTAG 1 cut(s) 327
BfmI CTRYAG 2 cut(s) 349, 602
BfuAI ACCTGC 1 cut(s) 595
BisI GCNGC 3 cut(s) 7, 10, 711
BlsI GCNGC 3 cut(s) 8, 11, 712
Bme1390I CCNGG 1 cut(s) 233
BmiI GGNNCC 1 cut(s) 757
BmrFI CCNGG 1 cut(s) 233
BpiI GAAGAC 1 cut(s) 219
Bpu10I CCTNAGC 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 209
BsaHI GRCGYC 1 cut(s) 139
BsaJI CCNNGG 2 cut(s) 18, 142
BsaWI WCCGGW 1 cut(s) 422
BsaXI ACNNNNNCTCC 2 cut(s) 168, 198
Bsc4I CCNNNNNNNGG 5 cut(s) 352, 353, 354, 422, 437
BseBI CCWGG 1 cut(s) 233
BseDI CCNNGG 2 cut(s) 18, 142
BseGI GGATG 1 cut(s) 481
BseLI CCNNNNNNNGG 5 cut(s) 352, 353, 354, 422, 437
BseMII CTCAG 1 cut(s) 369
BseRI GAGGAG 1 cut(s) 192
BseXI GCAGC 3 cut(s) 18, 21, 722
BshFI GGCC 5 cut(s) 231, 331, 430, 444, 639
BsiSI CCGG 2 cut(s) 423, 431
BslI CCNNNNNNNGG 5 cut(s) 352, 353, 354, 422, 437
BsmAI GTCTC 1 cut(s) 245
BsmI GAATGC 1 cut(s) 574
BsnI GGCC 5 cut(s) 231, 331, 430, 444, 639
Bsp143I GATC 2 cut(s) 82, 657
Bsp19I CCATGG 2 cut(s) 18, 142
BspANI GGCC 5 cut(s) 231, 331, 430, 444, 639
BspCNI CTCAG 1 cut(s) 370
BspLI GGNNCC 1 cut(s) 757
BspMAI CTGCAG 1 cut(s) 606
BspMI ACCTGC 1 cut(s) 595
BspPI GGATC 1 cut(s) 652
BspQI GCTCTTC 1 cut(s) 19
BssECI CCNNGG 2 cut(s) 18, 142
BssMI GATC 2 cut(s) 82, 657
BssNI GRCGYC 1 cut(s) 139
BssT1I CCWWGG 2 cut(s) 18, 142
Bst2UI CCWGG 1 cut(s) 233
Bst4CI ACNGT 1 cut(s) 689
Bst6I CTCTTC 1 cut(s) 19
BstACI GRCGYC 1 cut(s) 139
BstC8I GCNNGC 3 cut(s) 511, 515, 641
BstDEI CTNAG 4 cut(s) 168, 378, 483, 622
BstDSI CCRYGG 2 cut(s) 18, 142
BstF5I GGATG 1 cut(s) 481
BstKTI GATC 2 cut(s) 85, 660
BstMAI GTCTC 1 cut(s) 245
BstMBI GATC 2 cut(s) 82, 657
BstNI CCWGG 1 cut(s) 233
BstNSI RCATGY 1 cut(s) 707
BstSCI CCNGG 1 cut(s) 231
BstSFI CTRYAG 2 cut(s) 349, 602
BstV1I GCAGC 3 cut(s) 18, 21, 722
BstV2I GAAGAC 1 cut(s) 219
BstX2I RGATCY 1 cut(s) 657
BstXI CCANNNNNNTGG 1 cut(s) 251
BstYI RGATCY 1 cut(s) 657
BsuRI GGCC 5 cut(s) 231, 331, 430, 444, 639
BtgI CCRYGG 2 cut(s) 18, 142
BtsCI GGATG 1 cut(s) 481
BveI ACCTGC 1 cut(s) 595
Cac8I GCNNGC 3 cut(s) 511, 515, 641
CseI GACGC 1 cut(s) 147
Csp6I GTAC 1 cut(s) 185
CviAII CATG 6 cut(s) 19, 61, 143, 248, 437, 704
CviQI GTAC 1 cut(s) 185
DdeI CTNAG 4 cut(s) 168, 378, 483, 622
DpnI GATC 2 cut(s) 84, 659
DpnII GATC 2 cut(s) 82, 657
Eam1104I CTCTTC 1 cut(s) 19
EarI CTCTTC 1 cut(s) 19
Eco130I CCWWGG 2 cut(s) 18, 142
Eco57I CTGAAG 2 cut(s) 231, 733
EcoRII CCWGG 1 cut(s) 231
EcoT14I CCWWGG 2 cut(s) 18, 142
ErhI CCWWGG 2 cut(s) 18, 142
FaeI CATG 6 cut(s) 22, 64, 146, 251, 440, 707
FalI AAGNNNNNCTT 2 cut(s) 483, 515
FatI CATG 6 cut(s) 18, 60, 142, 247, 436, 703
FauNDI CATATG 1 cut(s) 547
Fnu4HI GCNGC 3 cut(s) 7, 10, 711
FokI GGATG 1 cut(s) 488
Fsp4HI GCNGC 3 cut(s) 7, 10, 711
FspBI CTAG 1 cut(s) 327
GluI GCNGC 3 cut(s) 7, 10, 711
HaeIII GGCC 5 cut(s) 231, 331, 430, 444, 639
HapII CCGG 2 cut(s) 423, 431
HgaI GACGC 1 cut(s) 147
Hin1I GRCGYC 1 cut(s) 139
Hin1II CATG 6 cut(s) 22, 64, 146, 251, 440, 707
HindIII AAGCTT 1 cut(s) 515
HinfI GANTC 3 cut(s) 255, 385, 469
HpaII CCGG 2 cut(s) 423, 431
HphI GGTGA 2 cut(s) 25, 380
Hpy166II GTNNAC 1 cut(s) 582
Hpy188I TCNGA 3 cut(s) 297, 379, 694
Hpy188III TCNNGA 5 cut(s) 311, 473, 655, 661, 676
Hpy8I GTNNAC 1 cut(s) 582
HpyAV CCTTC 5 cut(s) 38, 214, 420, 558, 757
HpyCH4III ACNGT 1 cut(s) 689
HpyCH4V TGCA 4 cut(s) 589, 604, 685, 710
HpyF3I CTNAG 4 cut(s) 168, 378, 483, 622
Hsp92I GRCGYC 1 cut(s) 139
Hsp92II CATG 6 cut(s) 22, 64, 146, 251, 440, 707
Kzo9I GATC 2 cut(s) 82, 657
LguI GCTCTTC 1 cut(s) 19
LmnI GCTCC 2 cut(s) 397, 433
Lsp1109I GCAGC 3 cut(s) 18, 21, 722
MaeI CTAG 1 cut(s) 327
MaeIII GTNAC 2 cut(s) 157, 723
MalI GATC 2 cut(s) 84, 659
MboI GATC 2 cut(s) 82, 657
MboII GAAGA 2 cut(s) 6, 224
MflI RGATCY 1 cut(s) 657
MluCI AATT 4 cut(s) 117, 130, 590, 679
MmeI TCCRAC 3 cut(s) 94, 154, 232
MnlI CCTC 7 cut(s) 170, 270, 316, 426, 434, 537, 629
MslI CAYNNNNRTG 1 cut(s) 459
MspI CCGG 2 cut(s) 423, 431
MspR9I CCNGG 1 cut(s) 233
Mva1269I GAATGC 1 cut(s) 574
MvaI CCWGG 1 cut(s) 233
NcoI CCATGG 2 cut(s) 18, 142
NdeI CATATG 1 cut(s) 547
NdeII GATC 2 cut(s) 82, 657
NlaIII CATG 6 cut(s) 22, 64, 146, 251, 440, 707
NlaIV GGNNCC 1 cut(s) 757
NmuCI GTSAC 1 cut(s) 157
NspI RCATGY 1 cut(s) 707
OliI CACNNNNGTG 1 cut(s) 459
PciI ACATGT 1 cut(s) 703
PciSI GCTCTTC 1 cut(s) 19
PctI GAATGC 1 cut(s) 574
PfeI GAWTC 3 cut(s) 255, 385, 469
PkrI GCNGC 3 cut(s) 8, 11, 712
PscI ACATGT 1 cut(s) 703
Psp6I CCWGG 1 cut(s) 231
PspGI CCWGG 1 cut(s) 231
PspN4I GGNNCC 1 cut(s) 757
PstI CTGCAG 1 cut(s) 606
PsuI RGATCY 1 cut(s) 657
RsaI GTAC 1 cut(s) 186
RsaNI GTAC 1 cut(s) 185
RseI CAYNNNNRTG 1 cut(s) 459
SapI GCTCTTC 1 cut(s) 19
SatI GCNGC 3 cut(s) 7, 10, 711
Sau3AI GATC 2 cut(s) 82, 657
ScrFI CCNGG 1 cut(s) 233
SfcI CTRYAG 2 cut(s) 349, 602
SmiMI CAYNNNNRTG 1 cut(s) 459
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
Sse9I AATT 4 cut(s) 117, 130, 590, 679
SspMI CTAG 1 cut(s) 327
StyD4I CCNGG 1 cut(s) 231
StyI CCWWGG 2 cut(s) 18, 142
TaaI ACNGT 1 cut(s) 689
TaqI TCGA 2 cut(s) 285, 654
TasI AATT 4 cut(s) 117, 130, 590, 679
TatI WGTACW 1 cut(s) 184
TfiI GAWTC 3 cut(s) 255, 385, 469
TseFI GTSAC 1 cut(s) 157
TseI GCWGC 3 cut(s) 6, 9, 710
Tsp45I GTSAC 1 cut(s) 157
TspDTI ATGAA 4 cut(s) 519, 529, 534, 680
XapI RAATTY 3 cut(s) 117, 130, 679
XceI RCATGY 1 cut(s) 707
XspI CTAG 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.