AT5G59320

Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005968
Physical Location & Seq
Forward (+)
23928954 .. 23929816
863 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G59320.1

Sequence Viewer

Length: 348 bp
ATGGCTTTCGCTTTGAGGTTCTTCACATGCCTTGTTTTAACGGTGTGCATAGTTGCATCAGTCGATGCTGCAATCTCATGTGGCACAGTGGCAGGTAGCTTGGCTCCATGTGCAACCTATCTATCAAAAGGTGGGTTGGTGCCACCTTCATGTTGTGCAGGAGTCAAAACTTTGAACAGTATGGCTAAAACCACACCAGACCGCCAACAAGCTTGCAGATGCATCCAGTCCACTGCGAAGAGCATTTCTGGTCTCAACCCAAGTCTAGCCTCTGGCCTTCCTGGAAAGTGCGGTGTTAGCATTCCATATCCAATCTCCATGAGCACTAACTGCAACAACATCAAGTGA

Protein Analysis

115

Amino Acids

11.69

Weight (kDa)

9.04

Isoelectric Point (pI)

42.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 27 - 111 2.3e-15 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 83
AccB1I GGYRCC 1 cut(s) 139
AciI CCGC 2 cut(s) 202, 291
AgsI TTSAA 1 cut(s) 175
AjnI CCWGG 1 cut(s) 280
AluBI AGCT 2 cut(s) 99, 212
AluI AGCT 2 cut(s) 99, 212
Alw21I GWGCWC 1 cut(s) 326
Alw26I GTCTC 1 cut(s) 257
AoxI GGCC 1 cut(s) 274
ApeKI GCWGC 1 cut(s) 68
BanI GGYRCC 1 cut(s) 139
Bbv12I GWGCWC 1 cut(s) 326
BbvI GCAGC 1 cut(s) 55
BciT130I CCWGG 1 cut(s) 282
BcoDI GTCTC 1 cut(s) 257
BfaI CTAG 1 cut(s) 266
BfuAI ACCTGC 1 cut(s) 83
BisI GCNGC 1 cut(s) 69
BlsI GCNGC 1 cut(s) 70
Bme1390I CCNGG 1 cut(s) 282
BmiI GGNNCC 2 cut(s) 105, 141
BmrFI CCNGG 1 cut(s) 282
BmsI GCATC 4 cut(s) 55, 65, 209, 231
BsaI GGTCTC 1 cut(s) 257
Bse1I ACTGG 1 cut(s) 226
BseBI CCWGG 1 cut(s) 282
BseGI GGATG 1 cut(s) 222
BseNI ACTGG 1 cut(s) 226
BseXI GCAGC 1 cut(s) 55
BsgI GTGCAG 1 cut(s) 177
BshFI GGCC 1 cut(s) 276
BshNI GGYRCC 1 cut(s) 139
BsiHKAI GWGCWC 1 cut(s) 326
BsmAI GTCTC 1 cut(s) 257
BsmI GAATGC 1 cut(s) 300
BsnI GGCC 1 cut(s) 276
Bso31I GGTCTC 1 cut(s) 257
Bsp1286I GDGCHC 1 cut(s) 326
BspACI CCGC 2 cut(s) 202, 291
BspANI GGCC 1 cut(s) 276
BspLI GGNNCC 2 cut(s) 105, 141
BspMI ACCTGC 1 cut(s) 83
BspQI GCTCTTC 1 cut(s) 233
BspT107I GGYRCC 1 cut(s) 139
BspTNI GGTCTC 1 cut(s) 257
BsrI ACTGG 1 cut(s) 226
Bst2UI CCWGG 1 cut(s) 282
Bst4CI ACNGT 3 cut(s) 43, 88, 179
Bst6I CTCTTC 1 cut(s) 233
BstAPI GCANNNNNTGC 1 cut(s) 330
BstC8I GCNNGC 1 cut(s) 214
BstF5I GGATG 1 cut(s) 222
BstMAI GTCTC 1 cut(s) 257
BstMWI GCNNNNNNNGC 3 cut(s) 110, 297, 330
BstNI CCWGG 1 cut(s) 282
BstNSI RCATGY 1 cut(s) 30
BstSCI CCNGG 1 cut(s) 280
BstV1I GCAGC 1 cut(s) 55
BsuRI GGCC 1 cut(s) 276
BtsCI GGATG 1 cut(s) 222
BtsI GCAGTG 1 cut(s) 231
BtsIMutI CAGTG 2 cut(s) 93, 231
BveI ACCTGC 1 cut(s) 83
Cac8I GCNNGC 1 cut(s) 214
CviAII CATG 5 cut(s) 27, 78, 108, 150, 319
CviJI RGCY 7 cut(s) 5, 99, 104, 185, 212, 269, 276
CviKI_1 RGCY 7 cut(s) 5, 99, 104, 185, 212, 269, 276
Eam1104I CTCTTC 1 cut(s) 233
EarI CTCTTC 1 cut(s) 233
Eco31I GGTCTC 1 cut(s) 257
EcoRII CCWGG 1 cut(s) 280
EcoT22I ATGCAT 1 cut(s) 224
FaeI CATG 5 cut(s) 30, 81, 111, 153, 322
FaiI YATR 8 cut(s) 28, 50, 79, 109, 151, 182, 307, 320
FatI CATG 5 cut(s) 26, 77, 107, 149, 318
Fnu4HI GCNGC 1 cut(s) 69
FokI GGATG 1 cut(s) 209
Fsp4HI GCNGC 1 cut(s) 69
FspBI CTAG 1 cut(s) 266
GluI GCNGC 1 cut(s) 69
HaeIII GGCC 1 cut(s) 276
Hin1II CATG 5 cut(s) 30, 81, 111, 153, 322
HindIII AAGCTT 1 cut(s) 210
HinfI GANTC 1 cut(s) 162
Hpy166II GTNNAC 1 cut(s) 231
Hpy8I GTNNAC 1 cut(s) 231
HpyAV CCTTC 2 cut(s) 156, 287
HpyCH4III ACNGT 3 cut(s) 43, 88, 179
HpyCH4V TGCA 8 cut(s) 48, 56, 71, 113, 158, 216, 222, 333
HpyF10VI GCNNNNNNNGC 3 cut(s) 110, 297, 330
Hsp92II CATG 5 cut(s) 30, 81, 111, 153, 322
LguI GCTCTTC 1 cut(s) 233
LmnI GCTCC 1 cut(s) 109
LpnPI CCDG 8 cut(s) 78, 144, 210, 234, 239, 258, 267, 294
Lsp1109I GCAGC 1 cut(s) 55
LweI GCATC 4 cut(s) 55, 65, 209, 231
MaeI CTAG 1 cut(s) 266
MboII GAAGA 2 cut(s) 13, 250
MhlI GDGCHC 1 cut(s) 326
MlyI GAGTC 1 cut(s) 171
MnlI CCTC 2 cut(s) 9, 280
Mph1103I ATGCAT 1 cut(s) 224
MseI TTAA 1 cut(s) 38
MslI CAYNNNNRTG 1 cut(s) 148
MspR9I CCNGG 1 cut(s) 282
Mva1269I GAATGC 1 cut(s) 300
MvaI CCWGG 1 cut(s) 282
MwoI GCNNNNNNNGC 3 cut(s) 110, 297, 330
NlaIII CATG 5 cut(s) 30, 81, 111, 153, 322
NlaIV GGNNCC 2 cut(s) 105, 141
NsiI ATGCAT 1 cut(s) 224
NspI RCATGY 1 cut(s) 30
PciSI GCTCTTC 1 cut(s) 233
PctI GAATGC 1 cut(s) 300
PfoI TCCNGGA 1 cut(s) 280
PkrI GCNGC 1 cut(s) 70
PleI GAGTC 1 cut(s) 170
PpsI GAGTC 1 cut(s) 170
Psp6I CCWGG 1 cut(s) 280
PspGI CCWGG 1 cut(s) 280
PspN4I GGNNCC 2 cut(s) 105, 141
RseI CAYNNNNRTG 1 cut(s) 148
SapI GCTCTTC 1 cut(s) 233
SaqAI TTAA 1 cut(s) 38
SatI GCNGC 1 cut(s) 69
SchI GAGTC 1 cut(s) 171
ScrFI CCNGG 1 cut(s) 282
SduI GDGCHC 1 cut(s) 326
SetI ASST 7 cut(s) 20, 97, 101, 119, 133, 148, 214
SfaNI GCATC 4 cut(s) 55, 65, 209, 231
SmiMI CAYNNNNRTG 1 cut(s) 148
SsiI CCGC 2 cut(s) 202, 291
SspMI CTAG 1 cut(s) 266
StyD4I CCNGG 1 cut(s) 280
TaaI ACNGT 3 cut(s) 43, 88, 179
TaqI TCGA 1 cut(s) 63
Tru1I TTAA 1 cut(s) 38
Tru9I TTAA 1 cut(s) 38
TscAI CASTG 2 cut(s) 93, 238
TseI GCWGC 1 cut(s) 68
TspDTI ATGAA 1 cut(s) 138
TspRI CASTG 2 cut(s) 93, 238
XceI RCATGY 1 cut(s) 30
XspI CTAG 1 cut(s) 266
Zsp2I ATGCAT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.