Rorug03G0044900

Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005968
Physical Location & Seq
Forward (+)
3473704 .. 3476415
2712 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0044900.1

Sequence Viewer

Length: 1098 bp
ATGGACAAGATAGCCGCGGAGTTACAGGACAGCGTGGTTGACCTGACCCGGCAGCTGACGGAGCTCCAGGCCCGGTATGAGTCGACCCGGAACCAGCAGCGGAGCAGAATGCAAGGTCTGGATCAGAGGGAGCTGCAATTGCAGGCCAGAGAGCTGAGGGTGAAGTCGGAGGAGGAAGCGATCCGGGAGCGGTGGAAGTGTCTGGAGATGAGAGAGAGGAATGCGGAAACACATGAGTTGAAGGAATTGGAAAGATTAGAGCATTTGGGTCAGCTTGAAAAATCGGTGGAAGAGAGGGAGAGGTCTTTGGATGAGCTACATGAGTCGTTGGAGGAGCAGAGGAAGCATTCCAGGGCACTACAGGAGGTGTTGGAAGAGCATTTGGGGTCGGTGGGCCGGGGGCTGAGGCAGTTTGAAGAGAAGGCTAGAGAAATTGGATTGGAGCTGAGTGATGGTGGTGGAGGATTGAAGTTGAAAAGAGGCGAGTTGGTGGGTTGCGAAATGGGAGGTGGTTTGGATGCAGAGAAGGTTGTCAAGCTTATTGGGGTGATTTGTCAGCGTGGAGAGATTATGGAGTTATGTCAGGGACTTGATTCTGGAGATAAGATCCACGATGTTATTCGAATTCTCATTGAAAGAAAGCAACTGATAGAGGCTGTTAGATTTATATGCACCTTCAAGTTAACTGACAAGTTCCCCCCTGTACCACTCTTAAACGAGTCTGTGGAGGATGCAGAGAAGTGGTGGAGTGAAACTTTCAGTCAAAAGAAATCACTTGATAAAAAGGGAAAAGCTGTAGATGACCGAATAACTAATCTAAGAGCTGTGGTTCAATGTGTTGAAGAATACAACCTACAGTCTGAGTATTCACTAGCAGACATTATCAATGACATTTTGGAGCTTGAAAAACTAAAGGAGAATCTGCATTCGGTGGCATCTCTTGACATTATGATGAAAACTAAAGGGCTCGGAAAACTAAAGGAGAGAAGGGAGGTGGTGCTATGTCTTCCATCCTTTGATGATAAACAAGGGAAGGAATTCTGGAGGAAACGAAGATTTCGCACATATCGCCGTTGCAGAAAAAAATGTAATCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

365

Amino Acids

42.54

Weight (kDa)

6.28

Isoelectric Point (pI)

47.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 172 - 309 5.2e-32 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 190
AccI GTMKAC 1 cut(s) 83
AccII CGCG 1 cut(s) 17
AciI CCGC 5 cut(s) 15, 17, 100, 190, 224
AclWI GGATC 3 cut(s) 129, 175, 601
AcsI RAATTY 2 cut(s) 624, 1037
AfaI GTAC 1 cut(s) 705
AjnI CCWGG 2 cut(s) 66, 350
Alw21I GWGCWC 1 cut(s) 66
AlwI GGATC 3 cut(s) 129, 175, 601
AoxI GGCC 3 cut(s) 69, 144, 394
ApeKI GCWGC 3 cut(s) 52, 97, 133
ApoI RAATTY 2 cut(s) 624, 1037
Asp700I GAANNNNTTC 1 cut(s) 1037
AspS9I GGNCC 2 cut(s) 70, 394
AsuC2I CCSGG 5 cut(s) 49, 73, 88, 185, 398
AsuHPI GGTGA 2 cut(s) 172, 559
AsuII TTCGAA 1 cut(s) 622
BaeGI GKGCMC 1 cut(s) 358
BanII GRGCYC 2 cut(s) 66, 969
BbsI GAAGAC 1 cut(s) 998
Bbv12I GWGCWC 1 cut(s) 66
BbvCI CCTCAGC 2 cut(s) 155, 404
BbvI GCAGC 3 cut(s) 64, 109, 120
BccI CCATC 2 cut(s) 446, 1018
BceAI ACGGC 1 cut(s) 1056
BciT130I CCWGG 2 cut(s) 68, 352
BcnI CCSGG 5 cut(s) 49, 73, 88, 185, 398
BfaI CTAG 2 cut(s) 426, 872
BfmI CTRYAG 3 cut(s) 359, 795, 854
BisI GCNGC 4 cut(s) 15, 53, 98, 134
BlsI GCNGC 4 cut(s) 16, 54, 99, 135
Bme1390I CCNGG 7 cut(s) 49, 68, 73, 88, 185, 352, 398
BmgT120I GGNCC 2 cut(s) 70, 394
BmiI GGNNCC 1 cut(s) 92
BmrFI CCNGG 7 cut(s) 49, 68, 73, 88, 185, 352, 398
BmsI GCATC 3 cut(s) 508, 721, 944
BpiI GAAGAC 1 cut(s) 998
BpmI CTGGAG 4 cut(s) 50, 224, 618, 1063
Bpu10I CCTNAGC 2 cut(s) 155, 404
Bpu14I TTCGAA 1 cut(s) 622
BpuMI CCSGG 5 cut(s) 49, 73, 88, 185, 398
BsaJI CCNNGG 3 cut(s) 15, 351, 397
BseBI CCWGG 2 cut(s) 68, 352
BseDI CCNNGG 3 cut(s) 15, 351, 397
BseGI GGATG 4 cut(s) 316, 523, 736, 1010
BseMII CTCAG 4 cut(s) 146, 395, 437, 852
BseRI GAGGAG 2 cut(s) 185, 347
BseSI GKGCMC 1 cut(s) 358
BseXI GCAGC 3 cut(s) 64, 109, 120
Bsh1236I CGCG 1 cut(s) 17
BshFI GGCC 3 cut(s) 71, 146, 396
BsiHKAI GWGCWC 1 cut(s) 66
BsiSI CCGG 5 cut(s) 49, 73, 88, 184, 397
BslFI GGGAC 1 cut(s) 600
BsmFI GGGAC 1 cut(s) 600
BsmI GAATGC 4 cut(s) 114, 226, 346, 925
BsnI GGCC 3 cut(s) 71, 146, 396
Bsp119I TTCGAA 1 cut(s) 622
Bsp1286I GDGCHC 3 cut(s) 66, 358, 969
Bsp143I GATC 3 cut(s) 121, 180, 606
BspACI CCGC 5 cut(s) 15, 17, 100, 190, 224
BspANI GGCC 3 cut(s) 71, 146, 396
BspCNI CTCAG 4 cut(s) 147, 396, 438, 853
BspFNI CGCG 1 cut(s) 17
BspLI GGNNCC 1 cut(s) 92
BspPI GGATC 3 cut(s) 129, 175, 601
BspQI GCTCTTC 1 cut(s) 369
BspT104I TTCGAA 1 cut(s) 622
BsrBI CCGCTC 1 cut(s) 190
BssECI CCNNGG 3 cut(s) 15, 351, 397
BssMI GATC 3 cut(s) 121, 180, 606
Bst2UI CCWGG 2 cut(s) 68, 352
Bst4CI ACNGT 1 cut(s) 858
Bst6I CTCTTC 3 cut(s) 285, 369, 411
BstBI TTCGAA 1 cut(s) 622
BstC8I GCNNGC 1 cut(s) 144
BstDEI CTNAG 5 cut(s) 155, 404, 446, 818, 861
BstDSI CCRYGG 1 cut(s) 15
BstF5I GGATG 4 cut(s) 316, 523, 736, 1010
BstFNI CGCG 1 cut(s) 17
BstKTI GATC 3 cut(s) 124, 183, 609
BstMBI GATC 3 cut(s) 121, 180, 606
BstMWI GCNNNNNNNGC 4 cut(s) 61, 139, 343, 1068
BstNI CCWGG 2 cut(s) 68, 352
BstSCI CCNGG 7 cut(s) 47, 66, 71, 86, 183, 350, 396
BstSFI CTRYAG 3 cut(s) 359, 795, 854
BstSLI GKGCMC 1 cut(s) 358
BstUI CGCG 1 cut(s) 17
BstV1I GCAGC 3 cut(s) 64, 109, 120
BstV2I GAAGAC 1 cut(s) 998
BstX2I RGATCY 1 cut(s) 606
BstYI RGATCY 1 cut(s) 606
BsuRI GGCC 3 cut(s) 71, 146, 396
BtgI CCRYGG 1 cut(s) 15
BtsCI GGATG 4 cut(s) 316, 523, 736, 1010
Cac8I GCNNGC 1 cut(s) 144
Cfr13I GGNCC 2 cut(s) 70, 394
Cfr42I CCGCGG 1 cut(s) 18
Csp6I GTAC 1 cut(s) 704
CviAII CATG 2 cut(s) 233, 320
CviQI GTAC 1 cut(s) 704
DdeI CTNAG 5 cut(s) 155, 404, 446, 818, 861
DpnI GATC 3 cut(s) 123, 182, 608
DpnII GATC 3 cut(s) 121, 180, 606
Eam1104I CTCTTC 3 cut(s) 285, 369, 411
EarI CTCTTC 3 cut(s) 285, 369, 411
Ecl136II GAGCTC 1 cut(s) 64
Eco24I GRGCYC 2 cut(s) 66, 969
Eco53kI GAGCTC 1 cut(s) 64
EcoICRI GAGCTC 1 cut(s) 64
EcoRI GAATTC 2 cut(s) 624, 1037
EcoRII CCWGG 2 cut(s) 66, 350
EcoT38I GRGCYC 2 cut(s) 66, 969
FaeI CATG 2 cut(s) 236, 323
FaqI GGGAC 1 cut(s) 600
FatI CATG 2 cut(s) 232, 319
FblI GTMKAC 1 cut(s) 83
Fnu4HI GCNGC 4 cut(s) 15, 53, 98, 134
FokI GGATG 4 cut(s) 323, 530, 743, 997
FriOI GRGCYC 2 cut(s) 66, 969
Fsp4HI GCNGC 4 cut(s) 15, 53, 98, 134
FspBI CTAG 2 cut(s) 426, 872
GluI GCNGC 4 cut(s) 15, 53, 98, 134
GsuI CTGGAG 4 cut(s) 50, 224, 618, 1063
HaeIII GGCC 3 cut(s) 71, 146, 396
HapII CCGG 5 cut(s) 49, 73, 88, 184, 397
Hin1II CATG 2 cut(s) 236, 323
HincII GTYRAC 3 cut(s) 40, 84, 684
HindII GTYRAC 3 cut(s) 40, 84, 684
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 5 cut(s) 80, 323, 593, 719, 919
HpaI GTTAAC 1 cut(s) 684
HpaII CCGG 5 cut(s) 49, 73, 88, 184, 397
HphI GGTGA 2 cut(s) 172, 559
Hpy166II GTNNAC 3 cut(s) 40, 84, 684
Hpy188I TCNGA 4 cut(s) 126, 169, 862, 971
Hpy188III TCNNGA 5 cut(s) 119, 203, 597, 941, 1042
Hpy8I GTNNAC 3 cut(s) 40, 84, 684
HpyAV CCTTC 6 cut(s) 235, 415, 520, 685, 981, 1027
HpyCH4III ACNGT 1 cut(s) 858
HpyCH4V TGCA 8 cut(s) 112, 136, 142, 521, 672, 734, 925, 1077
HpyF10VI GCNNNNNNNGC 4 cut(s) 61, 139, 343, 1068
HpyF3I CTNAG 5 cut(s) 155, 404, 446, 818, 861
Hsp92II CATG 2 cut(s) 236, 323
KspAI GTTAAC 1 cut(s) 684
KspI CCGCGG 1 cut(s) 18
Kzo9I GATC 3 cut(s) 121, 180, 606
LguI GCTCTTC 1 cut(s) 369
LmnI GCTCC 8 cut(s) 61, 69, 102, 130, 187, 334, 442, 898
Lsp1109I GCAGC 3 cut(s) 64, 109, 120
LweI GCATC 3 cut(s) 508, 721, 944
MaeI CTAG 2 cut(s) 426, 872
MaeIII GTNAC 1 cut(s) 21
MalI GATC 3 cut(s) 123, 182, 608
MbiI CCGCTC 1 cut(s) 190
MboI GATC 3 cut(s) 121, 180, 606
MboII GAAGA 6 cut(s) 302, 386, 428, 854, 998, 1065
MfeI CAATTG 1 cut(s) 137
MflI RGATCY 1 cut(s) 606
MhlI GDGCHC 3 cut(s) 66, 358, 969
MluCI AATT 5 cut(s) 137, 245, 432, 624, 1037
MlyI GAGTC 3 cut(s) 89, 332, 728
MmeI TCCRAC 3 cut(s) 147, 309, 351
MroXI GAANNNNTTC 1 cut(s) 1037
MseI TTAA 2 cut(s) 683, 713
MslI CAYNNNNRTG 1 cut(s) 950
MspA1I CMGCKG 3 cut(s) 17, 55, 100
MspI CCGG 5 cut(s) 49, 73, 88, 184, 397
MspR9I CCNGG 7 cut(s) 49, 68, 73, 88, 185, 352, 398
MunI CAATTG 1 cut(s) 137
Mva1269I GAATGC 4 cut(s) 114, 226, 346, 925
MvaI CCWGG 2 cut(s) 68, 352
MvnI CGCG 1 cut(s) 17
MwoI GCNNNNNNNGC 4 cut(s) 61, 139, 343, 1068
NciI CCSGG 5 cut(s) 49, 73, 88, 185, 398
NdeII GATC 3 cut(s) 121, 180, 606
NlaIII CATG 2 cut(s) 236, 323
NlaIV GGNNCC 1 cut(s) 92
NspV TTCGAA 1 cut(s) 622
PciSI GCTCTTC 1 cut(s) 369
PctI GAATGC 4 cut(s) 114, 226, 346, 925
PdmI GAANNNNTTC 1 cut(s) 1037
PfeI GAWTC 2 cut(s) 593, 919
PfoI TCCNGGA 1 cut(s) 183
PkrI GCNGC 4 cut(s) 16, 54, 99, 135
PleI GAGTC 3 cut(s) 88, 331, 727
PpsI GAGTC 3 cut(s) 88, 331, 727
Psp124BI GAGCTC 1 cut(s) 66
Psp6I CCWGG 2 cut(s) 66, 350
PspGI CCWGG 2 cut(s) 66, 350
PspN4I GGNNCC 1 cut(s) 92
PspPI GGNCC 2 cut(s) 70, 394
PsuI RGATCY 1 cut(s) 606
PvuII CAGCTG 1 cut(s) 55
RsaI GTAC 1 cut(s) 705
RsaNI GTAC 1 cut(s) 704
RseI CAYNNNNRTG 1 cut(s) 950
SacI GAGCTC 1 cut(s) 66
SacII CCGCGG 1 cut(s) 18
SalI GTCGAC 1 cut(s) 82
SapI GCTCTTC 1 cut(s) 369
SaqAI TTAA 2 cut(s) 683, 713
SatI GCNGC 4 cut(s) 15, 53, 98, 134
Sau3AI GATC 3 cut(s) 121, 180, 606
Sau96I GGNCC 2 cut(s) 70, 394
SchI GAGTC 3 cut(s) 89, 332, 728
ScrFI CCNGG 7 cut(s) 49, 68, 73, 88, 185, 352, 398
SduI GDGCHC 3 cut(s) 66, 358, 969
SfaNI GCATC 3 cut(s) 508, 721, 944
SfcI CTRYAG 3 cut(s) 359, 795, 854
Sfr303I CCGCGG 1 cut(s) 18
SfuI TTCGAA 1 cut(s) 622
SgrBI CCGCGG 1 cut(s) 18
SmiMI CAYNNNNRTG 1 cut(s) 950
Sse9I AATT 5 cut(s) 137, 245, 432, 624, 1037
SsiI CCGC 5 cut(s) 15, 17, 100, 190, 224
SspMI CTAG 2 cut(s) 426, 872
SstI GAGCTC 1 cut(s) 66
StyD4I CCNGG 7 cut(s) 47, 66, 71, 86, 183, 350, 396
TaaI ACNGT 1 cut(s) 858
TaqI TCGA 2 cut(s) 83, 622
TaqII GACCGA 1 cut(s) 819
TasI AATT 5 cut(s) 137, 245, 432, 624, 1037
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 2 cut(s) 593, 919
Tru1I TTAA 2 cut(s) 683, 713
Tru9I TTAA 2 cut(s) 683, 713
TseI GCWGC 3 cut(s) 52, 97, 133
TspDTI ATGAA 1 cut(s) 968
TspGWI ACGGA 2 cut(s) 74, 1083
XapI RAATTY 2 cut(s) 624, 1037
XmiI GTMKAC 1 cut(s) 83
XmnI GAANNNNTTC 1 cut(s) 1037
XspI CTAG 2 cut(s) 426, 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.