Rorug03G0044800

Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005968
Physical Location & Seq
Forward (+)
3470567 .. 3472235
1669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0044800.1

Sequence Viewer

Length: 843 bp
ATGAAGGGTTACATTTACCTACTGGAGACACTAATGAGAGTGTCAAAGCACATTGGACCTCATGTGAAAGAAGATGCAAGAAAGCTGGCAGTGCAGTGGAAAGCTAGAATGAGAACCGATGCTGGCAACTCATTGGAGATTTTACTATTTTTGGAGTTTGTGGCTACATATGGATTGCTCTCTACCATAAATGGAGGTGACATTGTCAATCTTCTTGGGGTGATTTCTCGGCATAGACAGGCTCTAGAACTATGTCAGTCGGTTGGCTTTGCAGATAAGATTCCTGGTTTTATTTGGAATCTCATTGAGAGGAAGCAACTAATTGATGCTGTTAGATGCATTTGCATCTTCAAGTTAATAGACAAGTTCCCTGCTGAGAAGGTTGTAGATGGTCTAATAGGTGATCTAAGAGCTGTGCATCAATGCATCAAAGACTACAATCTTGAGTCCAAATATCCATCTGCAGACATCGAAGTACAAGTAATTCAGCTGGGAAGACTGAAGGAGCATTACAGAAGTTTGGCGCCATCTCTTGCCTCCAGGGTTGATCAACAAGATCGGAGAAAAAGGAAGAGACCAAGCACCAGCACTTCTGCCCCCATCATTCAACCACATGAACAGCTTGAACAAAGTAATCACGAGACAGCTGTATCCACTGCTATACCCTATGCATTGCCGATTTCCACTTCAATTTGTCCAGAGTCAAGTTCGTTATCTAGGCTGAATGCAAATTATGGACTCCCTGGACAGTTTGGTATGGCAGCCAATGACCACGCAACCTGTGCTAATTCTGAAGGTGGCTTGATGCTAACATTGACAGGCCAATATGGATCGAATGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

280

Amino Acids

31.05

Weight (kDa)

8.68

Isoelectric Point (pI)

39.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 5 - 126 6e-31 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 523
AclWI GGATC 1 cut(s) 838
AcuI CTGAAG 2 cut(s) 521, 813
AcyI GRCGYC 1 cut(s) 524
AfaI GTAC 1 cut(s) 477
AgsI TTSAA 4 cut(s) 352, 608, 626, 690
AjnI CCWGG 3 cut(s) 283, 539, 742
AluBI AGCT 6 cut(s) 85, 104, 413, 490, 622, 647
AluI AGCT 6 cut(s) 85, 104, 413, 490, 622, 647
Alw26I GTCTC 3 cut(s) 20, 568, 635
AlwI GGATC 1 cut(s) 838
AoxI GGCC 1 cut(s) 820
ApeKI GCWGC 1 cut(s) 761
AspLEI GCGC 1 cut(s) 526
AspS9I GGNCC 1 cut(s) 56
AsuHPI GGTGA 3 cut(s) 209, 232, 413
AvaII GGWCC 1 cut(s) 56
BaeI ACNNNNGTAYC 2 cut(s) 633, 666
BanI GGYRCC 1 cut(s) 523
BarI GAAGNNNNNNTAC 2 cut(s) 494, 526
BauI CACGAG 1 cut(s) 638
BbsI GAAGAC 1 cut(s) 502
BbvI GCAGC 1 cut(s) 773
BccI CCATC 4 cut(s) 383, 466, 535, 608
BciT130I CCWGG 3 cut(s) 285, 541, 744
BciVI GTATCC 1 cut(s) 661
BclI TGATCA 1 cut(s) 547
BcoDI GTCTC 3 cut(s) 20, 568, 635
BfaI CTAG 3 cut(s) 105, 245, 717
BfmI CTRYAG 1 cut(s) 462
BfoI RGCGCY 1 cut(s) 527
BfuI GTATCC 1 cut(s) 661
BisI GCNGC 1 cut(s) 762
BlsI GCNGC 1 cut(s) 763
Bme1390I CCNGG 3 cut(s) 285, 541, 744
Bme18I GGWCC 1 cut(s) 56
BmgT120I GGNCC 1 cut(s) 56
BmiI GGNNCC 1 cut(s) 525
BmrFI CCNGG 3 cut(s) 285, 541, 744
BmsI GCATC 8 cut(s) 64, 109, 316, 326, 354, 427, 435, 795
BpiI GAAGAC 1 cut(s) 502
BpmI CTGGAG 2 cut(s) 44, 523
BpuEI CTTGAG 1 cut(s) 464
BsaHI GRCGYC 1 cut(s) 524
BsaI GGTCTC 1 cut(s) 568
BsaJI CCNNGG 2 cut(s) 540, 742
Bse1I ACTGG 1 cut(s) 27
Bse3DI GCAATG 1 cut(s) 671
BseBI CCWGG 3 cut(s) 285, 541, 744
BseDI CCNNGG 2 cut(s) 540, 742
BseMI GCAATG 1 cut(s) 671
BseMII CTCAG 1 cut(s) 366
BseNI ACTGG 1 cut(s) 27
BseXI GCAGC 1 cut(s) 773
BseYI CCCAGC 1 cut(s) 490
BsgI GTGCAG 1 cut(s) 113
BshFI GGCC 1 cut(s) 822
BshNI GGYRCC 1 cut(s) 523
BsmAI GTCTC 3 cut(s) 20, 568, 635
BsmI GAATGC 1 cut(s) 730
BsnI GGCC 1 cut(s) 822
Bso31I GGTCTC 1 cut(s) 568
Bsp143I GATC 4 cut(s) 403, 547, 556, 830
BspANI GGCC 1 cut(s) 822
BspCNI CTCAG 1 cut(s) 367
BspLI GGNNCC 1 cut(s) 525
BspMAI CTGCAG 1 cut(s) 466
BspPI GGATC 1 cut(s) 838
BspT107I GGYRCC 1 cut(s) 523
BspTNI GGTCTC 1 cut(s) 568
BsrDI GCAATG 1 cut(s) 671
BsrI ACTGG 1 cut(s) 27
BssECI CCNNGG 2 cut(s) 540, 742
BssMI GATC 4 cut(s) 403, 547, 556, 830
BssNI GRCGYC 1 cut(s) 524
BssSI CACGAG 1 cut(s) 638
Bst2BI CACGAG 1 cut(s) 638
Bst2UI CCWGG 3 cut(s) 285, 541, 744
Bst4CI ACNGT 1 cut(s) 750
Bst6I CTCTTC 1 cut(s) 566
BstACI GRCGYC 1 cut(s) 524
BstAPI GCANNNNNTGC 1 cut(s) 782
BstC8I GCNNGC 2 cut(s) 87, 124
BstDEI CTNAG 2 cut(s) 375, 407
BstH2I RGCGCY 1 cut(s) 527
BstHHI GCGC 1 cut(s) 526
BstKTI GATC 4 cut(s) 406, 550, 559, 833
BstMAI GTCTC 3 cut(s) 20, 568, 635
BstMBI GATC 4 cut(s) 403, 547, 556, 830
BstMWI GCNNNNNNNGC 2 cut(s) 91, 782
BstNI CCWGG 3 cut(s) 285, 541, 744
BstSCI CCNGG 3 cut(s) 283, 539, 742
BstSFI CTRYAG 1 cut(s) 462
BstV1I GCAGC 1 cut(s) 773
BstV2I GAAGAC 1 cut(s) 502
BsuI GTATCC 1 cut(s) 661
BsuRI GGCC 1 cut(s) 822
BtsI GCAGTG 3 cut(s) 96, 101, 654
BtsIMutI CAGTG 3 cut(s) 96, 101, 654
Cac8I GCNNGC 2 cut(s) 87, 124
CfoI GCGC 1 cut(s) 526
Cfr13I GGNCC 1 cut(s) 56
Csp6I GTAC 1 cut(s) 476
CviAII CATG 2 cut(s) 62, 614
CviQI GTAC 1 cut(s) 476
DdeI CTNAG 2 cut(s) 375, 407
DinI GGCGCC 1 cut(s) 525
DpnI GATC 4 cut(s) 405, 549, 558, 832
DpnII GATC 4 cut(s) 403, 547, 556, 830
Eam1104I CTCTTC 1 cut(s) 566
EarI CTCTTC 1 cut(s) 566
Eco31I GGTCTC 1 cut(s) 568
Eco47I GGWCC 1 cut(s) 56
Eco57I CTGAAG 2 cut(s) 521, 813
EcoRII CCWGG 3 cut(s) 283, 539, 742
EcoT22I ATGCAT 3 cut(s) 341, 428, 673
EgeI GGCGCC 1 cut(s) 525
EheI GGCGCC 1 cut(s) 525
FaeI CATG 2 cut(s) 65, 617
FatI CATG 2 cut(s) 61, 613
FauNDI CATATG 1 cut(s) 169
FbaI TGATCA 1 cut(s) 547
Fnu4HI GCNGC 1 cut(s) 762
Fsp4HI GCNGC 1 cut(s) 762
FspBI CTAG 3 cut(s) 105, 245, 717
GlaI GCGC 1 cut(s) 525
GluI GCNGC 1 cut(s) 762
GsaI CCCAGC 1 cut(s) 494
GsuI CTGGAG 2 cut(s) 44, 523
HaeII RGCGCY 1 cut(s) 527
HaeIII GGCC 1 cut(s) 822
HhaI GCGC 1 cut(s) 526
Hin1I GRCGYC 1 cut(s) 524
Hin1II CATG 2 cut(s) 65, 617
Hin6I GCGC 1 cut(s) 524
HinP1I GCGC 1 cut(s) 524
HinfI GANTC 5 cut(s) 280, 298, 446, 701, 738
HphI GGTGA 3 cut(s) 209, 232, 413
Hpy188I TCNGA 3 cut(s) 561, 793, 842
Hpy188III TCNNGA 4 cut(s) 245, 443, 638, 698
HpyAV CCTTC 3 cut(s) 373, 496, 788
HpyCH4III ACNGT 1 cut(s) 750
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 782
HpyF3I CTNAG 2 cut(s) 375, 407
Hsp92I GRCGYC 1 cut(s) 524
Hsp92II CATG 2 cut(s) 65, 617
HspAI GCGC 1 cut(s) 524
KasI GGCGCC 1 cut(s) 523
Ksp22I TGATCA 1 cut(s) 547
Kzo9I GATC 4 cut(s) 403, 547, 556, 830
LmnI GCTCC 1 cut(s) 505
Lsp1109I GCAGC 1 cut(s) 773
LweI GCATC 8 cut(s) 64, 109, 316, 326, 354, 427, 435, 795
MaeI CTAG 3 cut(s) 105, 245, 717
MaeIII GTNAC 2 cut(s) 8, 197
MalI GATC 4 cut(s) 405, 549, 558, 832
MboI GATC 4 cut(s) 403, 547, 556, 830
MboII GAAGA 5 cut(s) 83, 203, 340, 507, 583
MluCI AATT 5 cut(s) 321, 483, 690, 730, 787
Mly113I GGCGCC 1 cut(s) 524
MlyI GAGTC 3 cut(s) 455, 710, 732
MnlI CCTC 4 cut(s) 69, 188, 303, 547
Mph1103I ATGCAT 3 cut(s) 341, 428, 673
MseI TTAA 1 cut(s) 356
MspA1I CMGCKG 2 cut(s) 490, 647
MspR9I CCNGG 3 cut(s) 285, 541, 744
Mva1269I GAATGC 1 cut(s) 730
MvaI CCWGG 3 cut(s) 285, 541, 744
MwoI GCNNNNNNNGC 2 cut(s) 91, 782
NarI GGCGCC 1 cut(s) 524
NdeI CATATG 1 cut(s) 169
NdeII GATC 4 cut(s) 403, 547, 556, 830
NlaIII CATG 2 cut(s) 65, 617
NlaIV GGNNCC 1 cut(s) 525
NmeAIII GCCGAG 1 cut(s) 208
NmuCI GTSAC 1 cut(s) 197
NsiI ATGCAT 3 cut(s) 341, 428, 673
PctI GAATGC 1 cut(s) 730
PfeI GAWTC 2 cut(s) 280, 298
PflFI GACNNNGTC 1 cut(s) 203
PkrI GCNGC 1 cut(s) 763
PleI GAGTC 3 cut(s) 454, 709, 732
PluTI GGCGCC 1 cut(s) 527
PpsI GAGTC 3 cut(s) 454, 709, 732
Psp6I CCWGG 3 cut(s) 283, 539, 742
PspFI CCCAGC 1 cut(s) 490
PspGI CCWGG 3 cut(s) 283, 539, 742
PspN4I GGNNCC 1 cut(s) 525
PspPI GGNCC 1 cut(s) 56
PstI CTGCAG 1 cut(s) 466
PsyI GACNNNGTC 1 cut(s) 203
PvuII CAGCTG 2 cut(s) 490, 647
RsaI GTAC 1 cut(s) 477
RsaNI GTAC 1 cut(s) 476
SaqAI TTAA 1 cut(s) 356
SatI GCNGC 1 cut(s) 762
Sau3AI GATC 4 cut(s) 403, 547, 556, 830
Sau96I GGNCC 1 cut(s) 56
SchI GAGTC 3 cut(s) 455, 710, 732
ScrFI CCNGG 3 cut(s) 285, 541, 744
SfaNI GCATC 8 cut(s) 64, 109, 316, 326, 354, 427, 435, 795
SfcI CTRYAG 1 cut(s) 462
SfoI GGCGCC 1 cut(s) 525
SinI GGWCC 1 cut(s) 56
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
Sse9I AATT 5 cut(s) 321, 483, 690, 730, 787
SspDI GGCGCC 1 cut(s) 523
SspMI CTAG 3 cut(s) 105, 245, 717
StyD4I CCNGG 3 cut(s) 283, 539, 742
TaaI ACNGT 1 cut(s) 750
TaqI TCGA 2 cut(s) 471, 833
TasI AATT 5 cut(s) 321, 483, 690, 730, 787
TatI WGTACW 1 cut(s) 475
TfiI GAWTC 2 cut(s) 280, 298
Tru1I TTAA 1 cut(s) 356
Tru9I TTAA 1 cut(s) 356
TscAI CASTG 3 cut(s) 96, 101, 661
TseFI GTSAC 1 cut(s) 197
TseI GCWGC 1 cut(s) 761
Tsp45I GTSAC 1 cut(s) 197
TspDTI ATGAA 2 cut(s) 17, 630
TspRI CASTG 3 cut(s) 96, 101, 661
Tth111I GACNNNGTC 1 cut(s) 203
VpaK11BI GGWCC 1 cut(s) 56
XbaI TCTAGA 1 cut(s) 244
XspI CTAG 3 cut(s) 105, 245, 717
Zsp2I ATGCAT 3 cut(s) 341, 428, 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.