Rorug03G0044700

Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005968
Physical Location & Seq
Forward (+)
3468249 .. 3470446
2198 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0044700.1

Sequence Viewer

Length: 1200 bp
ATGGAAGGGATTCAGAAATCGATGGTGGCGTATTCGGATAGGATTAAGTCGAAAGAGGGGGTTATTAGGGAGATGGAGTTGAAGGTGAAGGAGTTTGGGATGCATAAGAAGGCAATGGAGGAGTGGTGTTGTAAGGTTGAAGTGAAGAAGAGGGAACTTGAAGTGTGGGTGGAGAAGGTTGAGCCGAGGGAGAGAGAGTTTGAGCCGAGAGTTGAGGAACTTGATTTGATTGGTAAGAGGGTTAATGAGTGTCTCAATGAGGCTCAGTTGACATTGGAAAGGGATTTTCGTTTGCTTGAGGAAATGAGACAGGAGAATGTGAAGCATTTTGAGTTAGTTGAGAAGTCGGTGCAAGAACGTTCTCATGAACTTGAAATGAAAGAGAGGAGACTTGAAGAAAAGGCAAAAGAGCTTGACTTGAAACAAAAACTATTGGAATCTATTCCGAAAGCTGTTGGAGAACAAATGAAATCGAAGGAGAAGGCTAGTATTGTTCATCCTTTAGTGAAGATTCAACATATGTTCCCTGCAAACAATGTTGCCGTTCCTTCAACAGCAAGTAATGCTAGAGGTTTGCAGTTGATCATGAGTGAGCATTTGAAGAGAATTGATTTAATGAGTAGAGAAATATCAGCTCTTCTTCAAGCCTCAAGAGACCCAGCAGGATTGGTTTTGGATGCAATGCAGGGGTTTTACCCTACAAATTCAACTGTGGACAACAGGGAGCTTGATTCGGGTCTAAGAGTGATTAGAAGGAGTTGTATTGTATTGCTACAGGAGTTAAAGAGATTCTCGCCACAAATTAATGCTCAGGTCAGAGAAAAGGCAATGAAGTTAGCAGCTGAGTGGAAGGCTAAGTTGGCGGTGACCACTGAGAATAGGTTGGAGGTGTTGGGCTTCTTGCGGCTTGTTACTACGTATGAATTGACCTCCATTTATGATCCAAAGGAGCTTCACAGTCTTCTCTCTATAGTTGTTAAGCCCGAACAAACAACTGAATTCCAGGCCCTTGGTGTCTCAGATAAGGCATTTGCGAGCAGCACAATTTCTTTCCCAGTTAGAATTGAGGAACCGGAATCTTCAGTGGCCAAATGTGTAGCCCCCTTTTCATCTCCAAATCTTCAACTAAGTGCAACCAGAGAACCAACAAATTTTCAGGGGTTCATAGTTGAGCGTTTGAGTGAGAATAATTCAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

399

Amino Acids

45.76

Weight (kDa)

6.47

Isoelectric Point (pI)

45.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 188 - 334 1.1e-29 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 863, 904
AclI AACGTT 1 cut(s) 358
AclWI GGATC 1 cut(s) 935
AcoI YGGCCR 1 cut(s) 1086
AcsI RAATTY 3 cut(s) 703, 998, 1150
AcuI CTGAAG 1 cut(s) 1065
AjnI CCWGG 1 cut(s) 1002
AjuI GAANNNNNNNTTGG 2 cut(s) 842, 874
AluBI AGCT 6 cut(s) 412, 452, 635, 727, 842, 952
AluI AGCT 6 cut(s) 412, 452, 635, 727, 842, 952
Alw26I GTCTC 5 cut(s) 257, 301, 382, 648, 1021
AlwI GGATC 1 cut(s) 935
AoxI GGCC 2 cut(s) 1005, 1086
ApeKI GCWGC 2 cut(s) 839, 1038
ApoI RAATTY 3 cut(s) 703, 998, 1150
AseI ATTAAT 1 cut(s) 804
Asp700I GAANNNNTTC 2 cut(s) 9, 441
AspS9I GGNCC 1 cut(s) 1006
AsuHPI GGTGA 2 cut(s) 97, 877
BalI TGGCCA 1 cut(s) 1088
BbsI GAAGAC 1 cut(s) 953
BbvI GCAGC 2 cut(s) 851, 1050
BccI CCATC 2 cut(s) 16, 67
BceAI ACGGC 1 cut(s) 527
BciT130I CCWGG 1 cut(s) 1004
BclI TGATCA 1 cut(s) 582
BcoDI GTCTC 5 cut(s) 257, 301, 382, 648, 1021
BfaI CTAG 2 cut(s) 486, 567
BfmI CTRYAG 2 cut(s) 773, 969
BisI GCNGC 3 cut(s) 840, 905, 1039
BlsI GCNGC 3 cut(s) 841, 906, 1040
Bme1390I CCNGG 1 cut(s) 1004
BmgT120I GGNCC 1 cut(s) 1006
BmiI GGNNCC 1 cut(s) 1071
BmrFI CCNGG 1 cut(s) 1004
BmrI ACTGGG 1 cut(s) 1049
BmsI GCATC 2 cut(s) 90, 667
BmuI ACTGGG 1 cut(s) 1049
BpiI GAAGAC 1 cut(s) 953
Bpu10I CCTNAGC 1 cut(s) 810
BpuEI CTTGAG 2 cut(s) 317, 634
Bsa29I ATCGAT 1 cut(s) 20
BsaAI YACGTR 1 cut(s) 918
BsaI GGTCTC 1 cut(s) 648
BsaJI CCNNGG 2 cut(s) 185, 1009
BsaWI WCCGGW 1 cut(s) 1072
BsaXI ACNNNNNCTCC 6 cut(s) 62, 92, 110, 140, 182, 212
Bse1I ACTGG 1 cut(s) 1055
Bse3DI GCAATG 3 cut(s) 120, 687, 834
BseBI CCWGG 1 cut(s) 1004
BseCI ATCGAT 1 cut(s) 20
BseDI CCNNGG 2 cut(s) 185, 1009
BseGI GGATG 3 cut(s) 105, 496, 682
BseMI GCAATG 3 cut(s) 120, 687, 834
BseMII CTCAG 5 cut(s) 278, 824, 834, 864, 1032
BseNI ACTGG 1 cut(s) 1055
BseRI GAGGAG 2 cut(s) 134, 400
BseXI GCAGC 2 cut(s) 851, 1050
BseYI CCCAGC 1 cut(s) 658
BshFI GGCC 2 cut(s) 1007, 1088
BshVI ATCGAT 1 cut(s) 20
BsiSI CCGG 1 cut(s) 1073
BsmAI GTCTC 5 cut(s) 257, 301, 382, 648, 1021
BsnI GGCC 2 cut(s) 1007, 1088
Bso31I GGTCTC 1 cut(s) 648
Bsp143I GATC 2 cut(s) 582, 940
BspACI CCGC 2 cut(s) 863, 904
BspANI GGCC 2 cut(s) 1007, 1088
BspCNI CTCAG 5 cut(s) 277, 823, 835, 865, 1031
BspDI ATCGAT 1 cut(s) 20
BspHI TCATGA 2 cut(s) 364, 585
BspLI GGNNCC 1 cut(s) 1071
BspPI GGATC 1 cut(s) 935
BspQI GCTCTTC 1 cut(s) 642
BspTNI GGTCTC 1 cut(s) 648
BsrDI GCAATG 3 cut(s) 120, 687, 834
BsrI ACTGG 1 cut(s) 1055
BssECI CCNNGG 2 cut(s) 185, 1009
BssMI GATC 2 cut(s) 582, 940
BssT1I CCWWGG 1 cut(s) 1009
Bst2UI CCWGG 1 cut(s) 1004
Bst4CI ACNGT 2 cut(s) 712, 959
Bst6I CTCTTC 3 cut(s) 143, 596, 642
BstAPI GCANNNNNTGC 1 cut(s) 563
BstBAI YACGTR 1 cut(s) 918
BstC8I GCNNGC 1 cut(s) 1036
BstDEI CTNAG 8 cut(s) 264, 740, 810, 843, 855, 873, 1018, 1127
BstEII GGTNACC 1 cut(s) 865
BstF5I GGATG 3 cut(s) 105, 496, 682
BstKTI GATC 2 cut(s) 585, 943
BstMAI GTCTC 5 cut(s) 257, 301, 382, 648, 1021
BstMBI GATC 2 cut(s) 582, 940
BstMWI GCNNNNNNNGC 2 cut(s) 563, 860
BstNI CCWGG 1 cut(s) 1004
BstPI GGTNACC 1 cut(s) 865
BstSCI CCNGG 1 cut(s) 1002
BstSFI CTRYAG 2 cut(s) 773, 969
BstSNI TACGTA 1 cut(s) 918
BstV1I GCAGC 2 cut(s) 851, 1050
BstV2I GAAGAC 1 cut(s) 953
BstXI CCANNNNNNTGG 1 cut(s) 1010
Bsu15I ATCGAT 1 cut(s) 20
BsuRI GGCC 2 cut(s) 1007, 1088
BsuTUI ATCGAT 1 cut(s) 20
BtsCI GGATG 3 cut(s) 105, 496, 682
BtsIMutI CAGTG 2 cut(s) 870, 1089
Cac8I GCNNGC 1 cut(s) 1036
CciI TCATGA 2 cut(s) 364, 585
Cfr13I GGNCC 1 cut(s) 1006
ClaI ATCGAT 1 cut(s) 20
CviAII CATG 2 cut(s) 365, 586
DdeI CTNAG 8 cut(s) 264, 740, 810, 843, 855, 873, 1018, 1127
DpnI GATC 2 cut(s) 584, 942
DpnII GATC 2 cut(s) 582, 940
EaeI YGGCCR 1 cut(s) 1086
Eam1104I CTCTTC 3 cut(s) 143, 596, 642
EarI CTCTTC 3 cut(s) 143, 596, 642
Eco105I TACGTA 1 cut(s) 918
Eco130I CCWWGG 1 cut(s) 1009
Eco31I GGTCTC 1 cut(s) 648
Eco57I CTGAAG 1 cut(s) 1065
Eco91I GGTNACC 1 cut(s) 865
EcoO109I RGGNCCY 1 cut(s) 1006
EcoO65I GGTNACC 1 cut(s) 865
EcoRI GAATTC 1 cut(s) 998
EcoRII CCWGG 1 cut(s) 1002
EcoT14I CCWWGG 1 cut(s) 1009
EcoT22I ATGCAT 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 1009
FaeI CATG 2 cut(s) 368, 589
FatI CATG 2 cut(s) 364, 585
FauNDI CATATG 1 cut(s) 519
FbaI TGATCA 1 cut(s) 582
Fnu4HI GCNGC 3 cut(s) 840, 905, 1039
FokI GGATG 3 cut(s) 112, 483, 689
Fsp4HI GCNGC 3 cut(s) 840, 905, 1039
FspBI CTAG 2 cut(s) 486, 567
GluI GCNGC 3 cut(s) 840, 905, 1039
GsaI CCCAGC 1 cut(s) 662
HaeIII GGCC 2 cut(s) 1007, 1088
HapII CCGG 1 cut(s) 1073
Hin1II CATG 2 cut(s) 368, 589
HincII GTYRAC 1 cut(s) 270
HindII GTYRAC 1 cut(s) 270
HinfI GANTC 6 cut(s) 10, 437, 511, 731, 789, 1076
HpaII CCGG 1 cut(s) 1073
HphI GGTGA 2 cut(s) 97, 877
Hpy166II GTNNAC 2 cut(s) 270, 715
Hpy188I TCNGA 5 cut(s) 15, 37, 447, 818, 1021
Hpy188III TCNNGA 3 cut(s) 365, 586, 651
Hpy8I GTNNAC 2 cut(s) 270, 715
HpyAV CCTTC 9 cut(s) 76, 82, 103, 169, 469, 475, 558, 747, 844
HpyCH4III ACNGT 2 cut(s) 712, 959
HpyCH4IV ACGT 2 cut(s) 358, 917
HpyCH4V TGCA 7 cut(s) 103, 352, 530, 577, 680, 685, 1133
HpyF10VI GCNNNNNNNGC 2 cut(s) 563, 860
HpyF3I CTNAG 8 cut(s) 264, 740, 810, 843, 855, 873, 1018, 1127
HpySE526I ACGT 2 cut(s) 358, 917
Hsp92II CATG 2 cut(s) 368, 589
Ksp22I TGATCA 1 cut(s) 582
Kzo9I GATC 2 cut(s) 582, 940
LguI GCTCTTC 1 cut(s) 642
LmnI GCTCC 2 cut(s) 724, 949
Lsp1109I GCAGC 2 cut(s) 851, 1050
LweI GCATC 2 cut(s) 90, 667
MaeI CTAG 2 cut(s) 486, 567
MaeII ACGT 2 cut(s) 358, 917
MaeIII GTNAC 2 cut(s) 865, 910
MalI GATC 2 cut(s) 584, 942
MboI GATC 2 cut(s) 582, 940
MlsI TGGCCA 1 cut(s) 1088
MluCI AATT 9 cut(s) 606, 703, 801, 923, 998, 1044, 1062, 1150, 1189
MluNI TGGCCA 1 cut(s) 1088
MmeI TCCRAC 2 cut(s) 436, 864
Mox20I TGGCCA 1 cut(s) 1088
Mph1103I ATGCAT 1 cut(s) 105
MroXI GAANNNNTTC 2 cut(s) 9, 441
MscI TGGCCA 1 cut(s) 1088
MseI TTAA 6 cut(s) 45, 243, 614, 782, 804, 978
Msp20I TGGCCA 1 cut(s) 1088
MspA1I CMGCKG 1 cut(s) 842
MspI CCGG 1 cut(s) 1073
MspR9I CCNGG 1 cut(s) 1004
MvaI CCWGG 1 cut(s) 1004
MwoI GCNNNNNNNGC 2 cut(s) 563, 860
NdeI CATATG 1 cut(s) 519
NdeII GATC 2 cut(s) 582, 940
NlaIII CATG 2 cut(s) 368, 589
NlaIV GGNNCC 1 cut(s) 1071
NmeAIII GCCGAG 2 cut(s) 210, 231
NmuCI GTSAC 1 cut(s) 865
NsiI ATGCAT 1 cut(s) 105
PagI TCATGA 2 cut(s) 364, 585
PciSI GCTCTTC 1 cut(s) 642
PcsI WCGNNNNNNNCGW 1 cut(s) 26
PdmI GAANNNNTTC 2 cut(s) 9, 441
PfeI GAWTC 6 cut(s) 10, 437, 511, 731, 789, 1076
PkrI GCNGC 3 cut(s) 841, 906, 1040
Ppu21I YACGTR 1 cut(s) 918
PshBI ATTAAT 1 cut(s) 804
Psp1406I AACGTT 1 cut(s) 358
Psp6I CCWGG 1 cut(s) 1002
PspEI GGTNACC 1 cut(s) 865
PspFI CCCAGC 1 cut(s) 658
PspGI CCWGG 1 cut(s) 1002
PspN4I GGNNCC 1 cut(s) 1071
PspPI GGNCC 1 cut(s) 1006
PvuII CAGCTG 1 cut(s) 842
SapI GCTCTTC 1 cut(s) 642
SaqAI TTAA 6 cut(s) 45, 243, 614, 782, 804, 978
SatI GCNGC 3 cut(s) 840, 905, 1039
Sau3AI GATC 2 cut(s) 582, 940
Sau96I GGNCC 1 cut(s) 1006
ScrFI CCNGG 1 cut(s) 1004
SfaNI GCATC 2 cut(s) 90, 667
SfcI CTRYAG 2 cut(s) 773, 969
SmlI CTYRAG 2 cut(s) 296, 649
SmoI CTYRAG 2 cut(s) 296, 649
SnaBI TACGTA 1 cut(s) 918
Sse9I AATT 9 cut(s) 606, 703, 801, 923, 998, 1044, 1062, 1150, 1189
SsiI CCGC 2 cut(s) 863, 904
SspMI CTAG 2 cut(s) 486, 567
StyD4I CCNGG 1 cut(s) 1002
StyI CCWWGG 1 cut(s) 1009
TaaI ACNGT 2 cut(s) 712, 959
TaiI ACGT 2 cut(s) 361, 920
TaqI TCGA 3 cut(s) 20, 50, 473
TasI AATT 9 cut(s) 606, 703, 801, 923, 998, 1044, 1062, 1150, 1189
TauI GCSGC 1 cut(s) 907
TfiI GAWTC 6 cut(s) 10, 437, 511, 731, 789, 1076
Tru1I TTAA 6 cut(s) 45, 243, 614, 782, 804, 978
Tru9I TTAA 6 cut(s) 45, 243, 614, 782, 804, 978
TscAI CASTG 2 cut(s) 877, 1089
TseFI GTSAC 1 cut(s) 865
TseI GCWGC 2 cut(s) 839, 1038
Tsp45I GTSAC 1 cut(s) 865
TspDTI ATGAA 8 cut(s) 381, 392, 482, 485, 845, 936, 1098, 1153
TspRI CASTG 2 cut(s) 877, 1089
VspI ATTAAT 1 cut(s) 804
XapI RAATTY 3 cut(s) 703, 998, 1150
XmnI GAANNNNTTC 2 cut(s) 9, 441
XspI CTAG 2 cut(s) 486, 567
Zsp2I ATGCAT 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.