MD12G1187000.v1.1

Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005968
Physical Location & Seq
Forward (+)
26910717 .. 26911515
799 bp
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UTR
Exon/CDS
Intron
MD12G1187000.v1.1.491

Sequence Viewer

Length: 351 bp
ATGGCTAGCCCTAAGGTGGTTTGTGTTTTCTTAATGTGCATAGTGCTGGCCGCACCCTTGATCACTGGAGCAGCACTCACATGTGGCCAGATTAAAGTCGGTCTTGCGCCTTGCCTGGCCTACCTCCAGAATGGTGGCTCCCCCACTCCAGGCTGCTGCAGGGGCATCAAAGGCCTGGTCAGCTCAGCCACGACCACAGCTGATCGACAGAATGCTTGCAACTGCTTGAAAACAGTTGCTGCCCAAATCAAAACTATTAAACAAGCCAACGCGGCTAAACTCCCTTCCTTATGTGGCGCCAACATTCCCTACAAGATCAGCACCTCTACTAACTGCGCCAGTGTGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

117

Amino Acids

11.74

Weight (kDa)

9.36

Isoelectric Point (pI)

26.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 21 - 104 2.2e-06 Probable lipid transfer
Tryp_alpha_amyl PF00234 28 - 112 1.1e-12 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 296
AccII CGCG 1 cut(s) 272
AciI CCGC 2 cut(s) 51, 272
AcoI YGGCCR 2 cut(s) 48, 85
AcyI GRCGYC 1 cut(s) 297
AfiI CCNNNNNNNGG 2 cut(s) 16, 149
AflIII ACRYGT 1 cut(s) 80
AgsI TTSAA 1 cut(s) 229
AjnI CCWGG 3 cut(s) 114, 148, 174
AluBI AGCT 2 cut(s) 183, 200
AluI AGCT 2 cut(s) 183, 200
AlwNI CAGNNNCTG 1 cut(s) 239
AoxI GGCC 4 cut(s) 48, 85, 117, 172
ApeKI GCWGC 4 cut(s) 71, 153, 156, 239
ArsI GACNNNNNNTTYG 2 cut(s) 162, 194
AspLEI GCGC 3 cut(s) 109, 299, 338
AsuNHI GCTAGC 1 cut(s) 5
AxyI CCTNAGG 1 cut(s) 12
BalI TGGCCA 1 cut(s) 87
BanI GGYRCC 1 cut(s) 296
BbvI GCAGC 4 cut(s) 83, 140, 143, 226
BciT130I CCWGG 3 cut(s) 116, 150, 176
BclI TGATCA 1 cut(s) 60
BfaI CTAG 1 cut(s) 6
BfmI CTRYAG 1 cut(s) 157
BfoI RGCGCY 1 cut(s) 300
BglI GCCNNNNNGGC 1 cut(s) 272
BisI GCNGC 6 cut(s) 51, 72, 154, 157, 240, 273
BlpI GCTNAGC 1 cut(s) 184
BlsI GCNGC 6 cut(s) 52, 73, 155, 158, 241, 274
Bme1390I CCNGG 3 cut(s) 116, 150, 176
BmiI GGNNCC 2 cut(s) 139, 298
BmrFI CCNGG 3 cut(s) 116, 150, 176
BmsI GCATC 1 cut(s) 174
BmtI GCTAGC 1 cut(s) 9
BplI GAGNNNNNCTC 2 cut(s) 60, 92
BpmI CTGGAG 3 cut(s) 87, 110, 132
Bpu1102I GCTNAGC 1 cut(s) 184
BsaHI GRCGYC 1 cut(s) 297
Bsc4I CCNNNNNNNGG 2 cut(s) 16, 149
Bse1I ACTGG 2 cut(s) 70, 339
Bse21I CCTNAGG 1 cut(s) 12
BseBI CCWGG 3 cut(s) 116, 150, 176
BseLI CCNNNNNNNGG 2 cut(s) 16, 149
BseMII CTCAG 1 cut(s) 198
BseNI ACTGG 2 cut(s) 70, 339
BseXI GCAGC 4 cut(s) 83, 140, 143, 226
Bsh1236I CGCG 1 cut(s) 272
BshFI GGCC 4 cut(s) 50, 87, 119, 174
BshNI GGYRCC 1 cut(s) 296
BslI CCNNNNNNNGG 2 cut(s) 16, 149
BsmI GAATGC 1 cut(s) 217
BsnI GGCC 4 cut(s) 50, 87, 119, 174
Bsp143I GATC 3 cut(s) 60, 202, 315
Bsp1720I GCTNAGC 1 cut(s) 184
BspACI CCGC 2 cut(s) 51, 272
BspANI GGCC 4 cut(s) 50, 87, 119, 174
BspCNI CTCAG 1 cut(s) 197
BspFNI CGCG 1 cut(s) 272
BspLI GGNNCC 2 cut(s) 139, 298
BspMAI CTGCAG 1 cut(s) 161
BspOI GCTAGC 1 cut(s) 9
BspT107I GGYRCC 1 cut(s) 296
BsrI ACTGG 2 cut(s) 70, 339
BssMI GATC 3 cut(s) 60, 202, 315
BssNI GRCGYC 1 cut(s) 297
Bst2UI CCWGG 3 cut(s) 116, 150, 176
Bst4CI ACNGT 1 cut(s) 235
BstACI GRCGYC 1 cut(s) 297
BstC8I GCNNGC 3 cut(s) 7, 48, 217
BstDEI CTNAG 2 cut(s) 12, 184
BstFNI CGCG 1 cut(s) 272
BstH2I RGCGCY 1 cut(s) 300
BstHHI GCGC 3 cut(s) 109, 299, 338
BstKTI GATC 3 cut(s) 63, 205, 318
BstMBI GATC 3 cut(s) 60, 202, 315
BstMWI GCNNNNNNNGC 4 cut(s) 162, 171, 180, 272
BstNI CCWGG 3 cut(s) 116, 150, 176
BstNSI RCATGY 1 cut(s) 84
BstSCI CCNGG 3 cut(s) 114, 148, 174
BstSFI CTRYAG 1 cut(s) 157
BstUI CGCG 1 cut(s) 272
BstV1I GCAGC 4 cut(s) 83, 140, 143, 226
BstXI CCANNNNNNTGG 1 cut(s) 134
Bsu36I CCTNAGG 1 cut(s) 12
BsuRI GGCC 4 cut(s) 50, 87, 119, 174
BtsIMutI CAGTG 2 cut(s) 63, 346
Cac8I GCNNGC 3 cut(s) 7, 48, 217
CaiI CAGNNNCTG 1 cut(s) 239
CfoI GCGC 3 cut(s) 109, 299, 338
CviAII CATG 1 cut(s) 81
DdeI CTNAG 2 cut(s) 12, 184
DinI GGCGCC 1 cut(s) 298
DpnI GATC 3 cut(s) 62, 204, 317
DpnII GATC 3 cut(s) 60, 202, 315
EaeI YGGCCR 2 cut(s) 48, 85
Eco147I AGGCCT 1 cut(s) 174
Eco81I CCTNAGG 1 cut(s) 12
EcoRII CCWGG 3 cut(s) 114, 148, 174
EgeI GGCGCC 1 cut(s) 298
EheI GGCGCC 1 cut(s) 298
FaeI CATG 1 cut(s) 84
FaiI YATR 3 cut(s) 41, 82, 292
FalI AAGNNNNNCTT 2 cut(s) 87, 119
FatI CATG 1 cut(s) 80
FbaI TGATCA 1 cut(s) 60
Fnu4HI GCNGC 6 cut(s) 51, 72, 154, 157, 240, 273
Fsp4HI GCNGC 6 cut(s) 51, 72, 154, 157, 240, 273
FspBI CTAG 1 cut(s) 6
GlaI GCGC 3 cut(s) 108, 298, 337
GluI GCNGC 6 cut(s) 51, 72, 154, 157, 240, 273
GsuI CTGGAG 3 cut(s) 87, 110, 132
HaeII RGCGCY 1 cut(s) 300
HaeIII GGCC 4 cut(s) 50, 87, 119, 174
HhaI GCGC 3 cut(s) 109, 299, 338
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 1 cut(s) 84
Hin6I GCGC 3 cut(s) 107, 297, 336
HinP1I GCGC 3 cut(s) 107, 297, 336
Hpy188III TCNNGA 1 cut(s) 127
HpyAV CCTTC 1 cut(s) 294
HpyCH4III ACNGT 1 cut(s) 235
HpyCH4V TGCA 3 cut(s) 39, 159, 219
HpyF10VI GCNNNNNNNGC 4 cut(s) 162, 171, 180, 272
HpyF3I CTNAG 2 cut(s) 12, 184
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 1 cut(s) 84
HspAI GCGC 3 cut(s) 107, 297, 336
KasI GGCGCC 1 cut(s) 296
Ksp22I TGATCA 1 cut(s) 60
Kzo9I GATC 3 cut(s) 60, 202, 315
LmnI GCTCC 2 cut(s) 68, 143
Lsp1109I GCAGC 4 cut(s) 83, 140, 143, 226
LweI GCATC 1 cut(s) 174
MaeI CTAG 1 cut(s) 6
MalI GATC 3 cut(s) 62, 204, 317
MboI GATC 3 cut(s) 60, 202, 315
MlsI TGGCCA 1 cut(s) 87
MluNI TGGCCA 1 cut(s) 87
Mly113I GGCGCC 1 cut(s) 297
MnlI CCTC 2 cut(s) 134, 334
Mox20I TGGCCA 1 cut(s) 87
MscI TGGCCA 1 cut(s) 87
MseI TTAA 3 cut(s) 32, 93, 258
MslI CAYNNNNRTG 1 cut(s) 79
Msp20I TGGCCA 1 cut(s) 87
MspA1I CMGCKG 1 cut(s) 200
MspR9I CCNGG 3 cut(s) 116, 150, 176
Mva1269I GAATGC 1 cut(s) 217
MvaI CCWGG 3 cut(s) 116, 150, 176
MvnI CGCG 1 cut(s) 272
MwoI GCNNNNNNNGC 4 cut(s) 162, 171, 180, 272
NarI GGCGCC 1 cut(s) 297
NdeII GATC 3 cut(s) 60, 202, 315
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 1 cut(s) 84
NlaIV GGNNCC 2 cut(s) 139, 298
NspI RCATGY 1 cut(s) 84
PceI AGGCCT 1 cut(s) 174
PciI ACATGT 1 cut(s) 80
PctI GAATGC 1 cut(s) 217
PkrI GCNGC 6 cut(s) 52, 73, 155, 158, 241, 274
PluTI GGCGCC 1 cut(s) 300
PscI ACATGT 1 cut(s) 80
Psp6I CCWGG 3 cut(s) 114, 148, 174
PspGI CCWGG 3 cut(s) 114, 148, 174
PspN4I GGNNCC 2 cut(s) 139, 298
PstI CTGCAG 1 cut(s) 161
PstNI CAGNNNCTG 1 cut(s) 239
PvuII CAGCTG 1 cut(s) 200
RseI CAYNNNNRTG 1 cut(s) 79
SaqAI TTAA 3 cut(s) 32, 93, 258
SatI GCNGC 6 cut(s) 51, 72, 154, 157, 240, 273
Sau3AI GATC 3 cut(s) 60, 202, 315
ScrFI CCNGG 3 cut(s) 116, 150, 176
SetI ASST 5 cut(s) 18, 126, 185, 202, 326
SfaNI GCATC 1 cut(s) 174
SfcI CTRYAG 1 cut(s) 157
SfoI GGCGCC 1 cut(s) 298
SmiMI CAYNNNNRTG 1 cut(s) 79
SseBI AGGCCT 1 cut(s) 174
SsiI CCGC 2 cut(s) 51, 272
SspDI GGCGCC 1 cut(s) 296
SspMI CTAG 1 cut(s) 6
StuI AGGCCT 1 cut(s) 174
StyD4I CCNGG 3 cut(s) 114, 148, 174
TaaI ACNGT 1 cut(s) 235
TaqI TCGA 1 cut(s) 205
TaqII GACCGA 1 cut(s) 89
TauI GCSGC 2 cut(s) 53, 275
Tru1I TTAA 3 cut(s) 32, 93, 258
Tru9I TTAA 3 cut(s) 32, 93, 258
TscAI CASTG 2 cut(s) 70, 346
TseI GCWGC 4 cut(s) 71, 153, 156, 239
TspRI CASTG 2 cut(s) 70, 346
XceI RCATGY 1 cut(s) 84
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.