Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0005968

Physical Location & Seq

Genomic Coordinates Forward (+)

26502648 .. 26504076

Gene Structure 1429 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1173500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 354 bp

ATGGCTACCTCTAATCAGGTGGTTTGTGTTTTCTTGATGTGCATAGTGGTGGCTGCACCCTTGATCACCGAAGCAGCACTCACGTGTGGCCAGATTAAGAGCGGTCTTGTGCCTTGCCTGGCCTACCTTCAGAATGGTGGCTTCCCCACTCCAGGCTGCTGCGGTGGGATCAAAGGCCTGATCCGCTCAGCCACGACCACAGCTGATCGACAGAATGCTTGCAACTGCTTGAAAACAGTTGCTGGCCAAATCAAAACTATTAAACCAGCAAACGCGGCTAAACTCCCATCCTTATGTAGCGCCAACATTCCCTACAAGATCAGCACCTCCACTAACTGCGCCAGTGTGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Tryp_alpha_amyl

Protein Analysis

118

Amino Acids

11.97

Weight (kDa)

9.14

Isoelectric Point (pI)

28.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LTP_2	PF14368	14 - 105	7.1e-06	Probable lipid transfer
Tryp_alpha_amyl	PF00234	29 - 113	1.2e-11	Protease inhibitor/seed storage/LTP family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005968)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G15050 AT2G15050 AT2G15050 AT2G38530 AT2G38540 AT3G51600 AT5G59310 AT5G59320
fragaria_vesca	FvH4_6g09990
malus_domestica	MD04G1173500.v1.1 MD12G1187000.v1.1
prunus_persica	Prupe.6G292500_v2.0.a1
pyrus_communis	pycom12g17530
rosa_chinensis	RchiOBHm_Chr3g0460661
rosa_multiflora	Rmu_co8170460.1_g000001 Rmu_sc0001256.1_g000011 Rmu_sc0011069.1_g000011
rosa_roxburghii	Rroxscaffold_6G00419320
rosa_rugosa	Rorug03G0044600 Rorug03G0044700 Rorug03G0044800 Rorug03G0044900
rosa_samantha	Rh3AG102500 Rh3BG106200 Rh3CG108100 Rh3DG107700
rosa_wichuraiana	Rw3G008720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	2 cut(s)	102, 186
AccII	CGCG	1 cut(s)	275
AciI	CCGC	4 cut(s)	102, 162, 184, 275
AclWI	GGATC	2 cut(s)	175, 176
AcoI	YGGCCR	2 cut(s)	88, 244
AcuI	CTGAAG	1 cut(s)	113
AcvI	CACGTG	1 cut(s)	84
AfiI	CCNNNNNNNGG	1 cut(s)	152
AflIII	ACRYGT	1 cut(s)	83
AgsI	TTSAA	1 cut(s)	232
AjnI	CCWGG	2 cut(s)	117, 151
AleI	CACNNNNGTG	1 cut(s)	82
AluBI	AGCT	1 cut(s)	203
AluI	AGCT	1 cut(s)	203
AlwI	GGATC	2 cut(s)	175, 176
AlwNI	CAGNNNCTG	1 cut(s)	242
AoxI	GGCC	4 cut(s)	88, 120, 175, 244
ApeKI	GCWGC	4 cut(s)	53, 74, 156, 159
AspLEI	GCGC	2 cut(s)	302, 341
AsuHPI	GGTGA	1 cut(s)	58
BalI	TGGCCA	2 cut(s)	90, 246
BbrPI	CACGTG	1 cut(s)	84
BbvI	GCAGC	4 cut(s)	40, 86, 143, 146
BccI	CCATC	1 cut(s)	295
BciT130I	CCWGG	2 cut(s)	119, 153
BclI	TGATCA	1 cut(s)	63
BfoI	RGCGCY	1 cut(s)	303
BisI	GCNGC	5 cut(s)	54, 75, 157, 160, 276
BlpI	GCTNAGC	1 cut(s)	187
BlsI	GCNGC	5 cut(s)	55, 76, 158, 161, 277
Bme1390I	CCNGG	2 cut(s)	119, 153
BmrFI	CCNGG	2 cut(s)	119, 153
BpmI	CTGGAG	1 cut(s)	135
Bpu1102I	GCTNAGC	1 cut(s)	187
BsaAI	YACGTR	1 cut(s)	84
Bsc4I	CCNNNNNNNGG	1 cut(s)	152
Bse1I	ACTGG	1 cut(s)	342
BseBI	CCWGG	2 cut(s)	119, 153
BseGI	GGATG	1 cut(s)	287
BseLI	CCNNNNNNNGG	1 cut(s)	152
BseMII	CTCAG	1 cut(s)	201
BseNI	ACTGG	1 cut(s)	342
BseXI	GCAGC	4 cut(s)	40, 86, 143, 146
BsgI	GTGCAG	1 cut(s)	39
Bsh1236I	CGCG	1 cut(s)	275
BshFI	GGCC	4 cut(s)	90, 122, 177, 246
BslI	CCNNNNNNNGG	1 cut(s)	152
BsmI	GAATGC	1 cut(s)	220
BsnI	GGCC	4 cut(s)	90, 122, 177, 246
Bsp143I	GATC	5 cut(s)	63, 168, 180, 205, 318
Bsp1720I	GCTNAGC	1 cut(s)	187
BspACI	CCGC	4 cut(s)	102, 162, 184, 275
BspANI	GGCC	4 cut(s)	90, 122, 177, 246
BspCNI	CTCAG	1 cut(s)	200
BspFNI	CGCG	1 cut(s)	275
BspPI	GGATC	2 cut(s)	175, 176
BsrBI	CCGCTC	2 cut(s)	102, 186
BsrI	ACTGG	1 cut(s)	342
BssMI	GATC	5 cut(s)	63, 168, 180, 205, 318
Bst2UI	CCWGG	2 cut(s)	119, 153
Bst4CI	ACNGT	1 cut(s)	238
BstBAI	YACGTR	1 cut(s)	84
BstC8I	GCNNGC	2 cut(s)	220, 244
BstDEI	CTNAG	1 cut(s)	187
BstF5I	GGATG	1 cut(s)	287
BstFNI	CGCG	1 cut(s)	275
BstH2I	RGCGCY	1 cut(s)	303
BstHHI	GCGC	2 cut(s)	302, 341
BstKTI	GATC	5 cut(s)	66, 171, 183, 208, 321
BstMBI	GATC	5 cut(s)	63, 168, 180, 205, 318
BstMWI	GCNNNNNNNGC	2 cut(s)	183, 275
BstNI	CCWGG	2 cut(s)	119, 153
BstSCI	CCNGG	2 cut(s)	117, 151
BstUI	CGCG	1 cut(s)	275
BstV1I	GCAGC	4 cut(s)	40, 86, 143, 146
BsuRI	GGCC	4 cut(s)	90, 122, 177, 246
BtsCI	GGATG	1 cut(s)	287
BtsIMutI	CAGTG	1 cut(s)	349
Cac8I	GCNNGC	2 cut(s)	220, 244
CaiI	CAGNNNCTG	1 cut(s)	242
CfoI	GCGC	2 cut(s)	302, 341
DdeI	CTNAG	1 cut(s)	187
DpnI	GATC	5 cut(s)	65, 170, 182, 207, 320
DpnII	GATC	5 cut(s)	63, 168, 180, 205, 318
EaeI	YGGCCR	2 cut(s)	88, 244
Eco147I	AGGCCT	1 cut(s)	177
Eco57I	CTGAAG	1 cut(s)	113
Eco72I	CACGTG	1 cut(s)	84
EcoRII	CCWGG	2 cut(s)	117, 151
FaiI	YATR	2 cut(s)	44, 295
FbaI	TGATCA	1 cut(s)	63
Fnu4HI	GCNGC	5 cut(s)	54, 75, 157, 160, 276
FokI	GGATG	1 cut(s)	274
Fsp4HI	GCNGC	5 cut(s)	54, 75, 157, 160, 276
GlaI	GCGC	2 cut(s)	301, 340
GluI	GCNGC	5 cut(s)	54, 75, 157, 160, 276
GsuI	CTGGAG	1 cut(s)	135
HaeII	RGCGCY	1 cut(s)	303
HaeIII	GGCC	4 cut(s)	90, 122, 177, 246
HhaI	GCGC	2 cut(s)	302, 341
Hin6I	GCGC	2 cut(s)	300, 339
HinP1I	GCGC	2 cut(s)	300, 339
HphI	GGTGA	1 cut(s)	58
Hpy188I	TCNGA	1 cut(s)	132
Hpy188III	TCNNGA	1 cut(s)	34
HpyAV	CCTTC	1 cut(s)	137
HpyCH4III	ACNGT	1 cut(s)	238
HpyCH4IV	ACGT	1 cut(s)	83
HpyCH4V	TGCA	3 cut(s)	42, 56, 222
HpyF10VI	GCNNNNNNNGC	2 cut(s)	183, 275
HpyF3I	CTNAG	1 cut(s)	187
HpySE526I	ACGT	1 cut(s)	83
HspAI	GCGC	2 cut(s)	300, 339
Ksp22I	TGATCA	1 cut(s)	63
Kzo9I	GATC	5 cut(s)	63, 168, 180, 205, 318
LpnPI	CCDG	9 cut(s)	2, 104, 104, 131, 138, 165, 191, 228, 279
Lsp1109I	GCAGC	4 cut(s)	40, 86, 143, 146
MaeII	ACGT	1 cut(s)	83
MalI	GATC	5 cut(s)	65, 170, 182, 207, 320
MbiI	CCGCTC	2 cut(s)	102, 186
MboI	GATC	5 cut(s)	63, 168, 180, 205, 318
MlsI	TGGCCA	2 cut(s)	90, 246
MluNI	TGGCCA	2 cut(s)	90, 246
MnlI	CCTC	2 cut(s)	19, 337
Mox20I	TGGCCA	2 cut(s)	90, 246
MscI	TGGCCA	2 cut(s)	90, 246
MseI	TTAA	2 cut(s)	96, 261
MslI	CAYNNNNRTG	3 cut(s)	47, 82, 292
Msp20I	TGGCCA	2 cut(s)	90, 246
MspA1I	CMGCKG	1 cut(s)	203
MspR9I	CCNGG	2 cut(s)	119, 153
Mva1269I	GAATGC	1 cut(s)	220
MvaI	CCWGG	2 cut(s)	119, 153
MvnI	CGCG	1 cut(s)	275
MwoI	GCNNNNNNNGC	2 cut(s)	183, 275
NdeII	GATC	5 cut(s)	63, 168, 180, 205, 318
OliI	CACNNNNGTG	1 cut(s)	82
PceI	AGGCCT	1 cut(s)	177
PctI	GAATGC	1 cut(s)	220
PkrI	GCNGC	5 cut(s)	55, 76, 158, 161, 277
PmaCI	CACGTG	1 cut(s)	84
PmlI	CACGTG	1 cut(s)	84
Ppu21I	YACGTR	1 cut(s)	84
Psp6I	CCWGG	2 cut(s)	117, 151
PspCI	CACGTG	1 cut(s)	84
PspGI	CCWGG	2 cut(s)	117, 151
PstNI	CAGNNNCTG	1 cut(s)	242
PvuII	CAGCTG	1 cut(s)	203
RseI	CAYNNNNRTG	3 cut(s)	47, 82, 292
SaqAI	TTAA	2 cut(s)	96, 261
SatI	GCNGC	5 cut(s)	54, 75, 157, 160, 276
Sau3AI	GATC	5 cut(s)	63, 168, 180, 205, 318
ScrFI	CCNGG	2 cut(s)	119, 153
SetI	ASST	6 cut(s)	11, 21, 86, 129, 205, 329
SmiMI	CAYNNNNRTG	3 cut(s)	47, 82, 292
SseBI	AGGCCT	1 cut(s)	177
SsiI	CCGC	4 cut(s)	102, 162, 184, 275
StuI	AGGCCT	1 cut(s)	177
StyD4I	CCNGG	2 cut(s)	117, 151
TaaI	ACNGT	1 cut(s)	238
TaiI	ACGT	1 cut(s)	86
TaqI	TCGA	1 cut(s)	208
TauI	GCSGC	1 cut(s)	278
Tru1I	TTAA	2 cut(s)	96, 261
Tru9I	TTAA	2 cut(s)	96, 261
TscAI	CASTG	1 cut(s)	349
TseI	GCWGC	4 cut(s)	53, 74, 156, 159
TspRI	CASTG	1 cut(s)	349

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.