AT5G62440

Encodes a protein DOMINO1 that belongs to a plant-specific gene family sharing a common motif present in the tomato DEFECTIVE CHLOROPLASTS AND LEAVES (LeDCL) protein. DOMINO1 is located in the nucleus. Arabidopsis embryos carrying the domino1 mutation grow slowly in comparison with wild type embryos and reach only the globular stage at desiccation. The primary defect of the mutation at the cellular level is the large size of the nucleolus that can be observed soon after fertilization in the nuclei of both the embryo and the endosperm. DOMINO1 might have a role in ribosome biogenesis and in determining the rate of cell division

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0014579
Physical Location & Seq
Reverse (-)
25072117 .. 25074069
1953 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G62440.1

Sequence Viewer

Length: 609 bp
ATGGCTGAAGAACAAGAGATCGTCGATTCCCTGTCAGCGGAAGTGAACCCAGACCAGAAAGTGGACATGGAAGTCGAAACCGCGACCCCGAAAGCCGAGACCGGCGATGAGAAACGTGAGAGAGAGGAGACAGAGGAAGAAGAGAACGGAGGTGAATCGAAGAAGCAGAAGGTTGGAGAGGAAGAGAAGTCAGGTCCGGTTAAACTTGGTCCCAAGGAGTTTGTTACCTCTGTGGCGATGTTCGATTACTTTGTCAAGTTTTTACACTTTTGGCCAACTGATCTCGATGTTAATAAGTATGAGCACATGGTGCTGCTAGATCTGATTAAGAAGGGCCATTCTGAGCCTGAAAAGAAGATTGGAGGAGGAATCAAAACCTTCCAAGTAAGAACCCACCCAATGTGGAAAAGCAGATGCTTCTTTCTGGTTAGGGAAGACGATACTGCAGACGATTTCAGCTTCAGGAAGTGTGTTGATCAAATCCTCCCATTGCCTGAAAACATGAAGACTCCTGGAGCTAACGGCAATGGACATGGTGGAGGTCGCGGTGGCGGTGGTGGAAGAAGAGGCGGTCGTGGTGGTGGTAGAGGAGGGAGATTCAGAAGATGA

Protein Analysis

202

Amino Acids

22.54

Weight (kDa)

5.68

Isoelectric Point (pI)

37.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 86 - 158 2.1e-28 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62440
fragaria_vesca FvH4_5g14920
malus_domestica MD06G1193500.v1.1 MD14G1200600.v1.1
prunus_persica Prupe.5G193800_v2.0.a1
pyrus_communis pycom06g17160 pycom14g16630
rosa_chinensis RchiOBHm_Chr7g0181461
rosa_laevigata RLG00000005192
rosa_multiflora Rmu_sc0004621.1_g000022
rosa_roxburghii Rroxscaffold_3G00271980
rosa_rugosa Rorug06G0441500
rosa_samantha Rh7AG043300 Rh7BG042700 Rh7CG044500 Rh7DG043100
rosa_wichuraiana Rw7G003600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 71
AccB7I CCANNNNNTGG 1 cut(s) 61
AccII CGCG 2 cut(s) 83, 546
AciI CCGC 5 cut(s) 38, 81, 546, 552, 570
AcoI YGGCCR 1 cut(s) 272
AcuI CTGAAG 2 cut(s) 27, 445
AdeI CACNNNGTG 1 cut(s) 310
AfiI CCNNNNNNNGG 2 cut(s) 37, 61
AjnI CCWGG 1 cut(s) 511
AjuI GAANNNNNNNTTGG 2 cut(s) 342, 374
AluBI AGCT 2 cut(s) 459, 518
AluI AGCT 2 cut(s) 459, 518
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 2 cut(s) 92, 122
AoxI GGCC 2 cut(s) 272, 334
ApeKI GCWGC 1 cut(s) 313
AspS9I GGNCC 3 cut(s) 194, 209, 334
AsuHPI GGTGA 1 cut(s) 164
AvaII GGWCC 2 cut(s) 194, 209
BalI TGGCCA 1 cut(s) 274
BbsI GAAGAC 2 cut(s) 441, 512
Bbv12I GWGCWC 1 cut(s) 306
BbvI GCAGC 1 cut(s) 300
BceAI ACGGC 1 cut(s) 538
BciT130I CCWGG 1 cut(s) 513
BclI TGATCA 1 cut(s) 475
BcoDI GTCTC 2 cut(s) 92, 122
BfaI CTAG 1 cut(s) 317
BfmI CTRYAG 1 cut(s) 444
BglII AGATCT 1 cut(s) 319
BisI GCNGC 1 cut(s) 314
BlsI GCNGC 1 cut(s) 315
Bme1390I CCNGG 1 cut(s) 513
Bme18I GGWCC 2 cut(s) 194, 209
BmgT120I GGNCC 3 cut(s) 194, 209, 334
BmiI GGNNCC 1 cut(s) 211
BmrFI CCNGG 1 cut(s) 513
BmsI GCATC 1 cut(s) 404
BpiI GAAGAC 2 cut(s) 441, 512
BpmI CTGGAG 1 cut(s) 534
BsaI GGTCTC 1 cut(s) 92
BsaJI CCNNGG 1 cut(s) 213
BsaWI WCCGGW 1 cut(s) 196
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 61
Bse118I RCCGGY 1 cut(s) 101
Bse3DI GCAATG 2 cut(s) 488, 532
BseBI CCWGG 1 cut(s) 513
BseDI CCNNGG 1 cut(s) 213
BseLI CCNNNNNNNGG 2 cut(s) 37, 61
BseMI GCAATG 2 cut(s) 488, 532
BseMII CTCAG 1 cut(s) 333
BseRI GAGGAG 3 cut(s) 140, 378, 603
BseXI GCAGC 1 cut(s) 300
Bsh1236I CGCG 2 cut(s) 83, 546
Bsh1285I CGRYCG 1 cut(s) 574
BshFI GGCC 2 cut(s) 274, 336
BsiEI CGRYCG 1 cut(s) 574
BsiHKAI GWGCWC 1 cut(s) 306
BsiSI CCGG 2 cut(s) 102, 197
BslFI GGGAC 1 cut(s) 195
BslI CCNNNNNNNGG 2 cut(s) 37, 61
BsmAI GTCTC 2 cut(s) 92, 122
BsmFI GGGAC 1 cut(s) 195
BsnI GGCC 2 cut(s) 274, 336
Bso31I GGTCTC 1 cut(s) 92
Bsp1286I GDGCHC 1 cut(s) 306
Bsp143I GATC 4 cut(s) 18, 280, 319, 475
BspACI CCGC 5 cut(s) 38, 81, 546, 552, 570
BspANI GGCC 2 cut(s) 274, 336
BspCNI CTCAG 1 cut(s) 334
BspFNI CGCG 2 cut(s) 83, 546
BspLI GGNNCC 1 cut(s) 211
BspMAI CTGCAG 1 cut(s) 448
BspTNI GGTCTC 1 cut(s) 92
BsrDI GCAATG 2 cut(s) 488, 532
BsrFI RCCGGY 1 cut(s) 101
BssAI RCCGGY 1 cut(s) 101
BssECI CCNNGG 1 cut(s) 213
BssMI GATC 4 cut(s) 18, 280, 319, 475
BssT1I CCWWGG 1 cut(s) 213
Bst2UI CCWGG 1 cut(s) 513
Bst6I CTCTTC 3 cut(s) 135, 177, 559
BstAPI GCANNNNNTGC 1 cut(s) 310
BstDEI CTNAG 1 cut(s) 342
BstFNI CGCG 2 cut(s) 83, 546
BstKTI GATC 4 cut(s) 21, 283, 322, 478
BstMAI GTCTC 2 cut(s) 92, 122
BstMBI GATC 4 cut(s) 18, 280, 319, 475
BstMCI CGRYCG 1 cut(s) 574
BstMWI GCNNNNNNNGC 1 cut(s) 310
BstNI CCWGG 1 cut(s) 513
BstSCI CCNGG 1 cut(s) 511
BstSFI CTRYAG 1 cut(s) 444
BstUI CGCG 2 cut(s) 83, 546
BstV1I GCAGC 1 cut(s) 300
BstV2I GAAGAC 2 cut(s) 441, 512
BstX2I RGATCY 1 cut(s) 319
BstYI RGATCY 1 cut(s) 319
BsuRI GGCC 2 cut(s) 274, 336
BtgZI GCGATG 2 cut(s) 120, 251
Cfr10I RCCGGY 1 cut(s) 101
Cfr13I GGNCC 3 cut(s) 194, 209, 334
CviAII CATG 4 cut(s) 67, 307, 502, 533
CviJI RGCY 7 cut(s) 5, 95, 274, 336, 346, 459, 518
CviKI_1 RGCY 7 cut(s) 5, 95, 274, 336, 346, 459, 518
DdeI CTNAG 1 cut(s) 342
DpnI GATC 4 cut(s) 20, 282, 321, 477
DpnII GATC 4 cut(s) 18, 280, 319, 475
DraIII CACNNNGTG 1 cut(s) 310
DrdI GACNNNNNNGTC 1 cut(s) 71
DseDI GACNNNNNNGTC 1 cut(s) 71
EaeI YGGCCR 1 cut(s) 272
Eam1104I CTCTTC 3 cut(s) 135, 177, 559
EarI CTCTTC 3 cut(s) 135, 177, 559
Eco130I CCWWGG 1 cut(s) 213
Eco31I GGTCTC 1 cut(s) 92
Eco47I GGWCC 2 cut(s) 194, 209
Eco57I CTGAAG 2 cut(s) 27, 445
EcoRII CCWGG 1 cut(s) 511
EcoT14I CCWWGG 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 213
FaeI CATG 4 cut(s) 70, 310, 505, 536
FaiI YATR 5 cut(s) 68, 300, 308, 503, 534
FaqI GGGAC 1 cut(s) 195
FatI CATG 4 cut(s) 66, 306, 501, 532
FbaI TGATCA 1 cut(s) 475
Fnu4HI GCNGC 1 cut(s) 314
Fsp4HI GCNGC 1 cut(s) 314
FspBI CTAG 1 cut(s) 317
GluI GCNGC 1 cut(s) 314
GsuI CTGGAG 1 cut(s) 534
HaeIII GGCC 2 cut(s) 274, 336
HapII CCGG 2 cut(s) 102, 197
Hin1II CATG 4 cut(s) 70, 310, 505, 536
HinfI GANTC 5 cut(s) 26, 155, 369, 508, 597
HpaII CCGG 2 cut(s) 102, 197
HphI GGTGA 1 cut(s) 164
Hpy166II GTNNAC 2 cut(s) 46, 64
Hpy188I TCNGA 3 cut(s) 324, 343, 602
Hpy188III TCNNGA 2 cut(s) 284, 463
Hpy8I GTNNAC 2 cut(s) 46, 64
Hpy99I CGWCG 1 cut(s) 26
HpyAV CCTTC 3 cut(s) 163, 325, 388
HpyCH4IV ACGT 1 cut(s) 115
HpyCH4V TGCA 1 cut(s) 446
HpyF10VI GCNNNNNNNGC 1 cut(s) 310
HpyF3I CTNAG 1 cut(s) 342
HpySE526I ACGT 1 cut(s) 115
Hsp92II CATG 4 cut(s) 70, 310, 505, 536
Ksp22I TGATCA 1 cut(s) 475
Kzo9I GATC 4 cut(s) 18, 280, 319, 475
LmnI GCTCC 1 cut(s) 515
Lsp1109I GCAGC 1 cut(s) 300
LweI GCATC 1 cut(s) 404
MaeI CTAG 1 cut(s) 317
MaeII ACGT 1 cut(s) 115
MaeIII GTNAC 1 cut(s) 223
MalI GATC 4 cut(s) 20, 282, 321, 477
MboI GATC 4 cut(s) 18, 280, 319, 475
MflI RGATCY 1 cut(s) 319
MhlI GDGCHC 1 cut(s) 306
MlsI TGGCCA 1 cut(s) 274
MluNI TGGCCA 1 cut(s) 274
MlyI GAGTC 1 cut(s) 502
MmeI TCCRAC 1 cut(s) 154
Mox20I TGGCCA 1 cut(s) 274
MscI TGGCCA 1 cut(s) 274
MseI TTAA 3 cut(s) 201, 291, 327
Msp20I TGGCCA 1 cut(s) 274
MspA1I CMGCKG 1 cut(s) 38
MspI CCGG 2 cut(s) 102, 197
MspR9I CCNGG 1 cut(s) 513
MvaI CCWGG 1 cut(s) 513
MvnI CGCG 2 cut(s) 83, 546
MwoI GCNNNNNNNGC 1 cut(s) 310
NdeII GATC 4 cut(s) 18, 280, 319, 475
NlaIII CATG 4 cut(s) 70, 310, 505, 536
NlaIV GGNNCC 1 cut(s) 211
NmeAIII GCCGAG 1 cut(s) 121
PfeI GAWTC 4 cut(s) 26, 155, 369, 597
PflMI CCANNNNNTGG 1 cut(s) 61
PfoI TCCNGGA 1 cut(s) 511
PkrI GCNGC 1 cut(s) 315
PleI GAGTC 1 cut(s) 502
PpsI GAGTC 1 cut(s) 502
Psp6I CCWGG 1 cut(s) 511
PspGI CCWGG 1 cut(s) 511
PspN4I GGNNCC 1 cut(s) 211
PspPI GGNCC 3 cut(s) 194, 209, 334
PstI CTGCAG 1 cut(s) 448
PsuI RGATCY 1 cut(s) 319
SaqAI TTAA 3 cut(s) 201, 291, 327
SatI GCNGC 1 cut(s) 314
Sau3AI GATC 4 cut(s) 18, 280, 319, 475
Sau96I GGNCC 3 cut(s) 194, 209, 334
SchI GAGTC 1 cut(s) 502
ScrFI CCNGG 1 cut(s) 513
SduI GDGCHC 1 cut(s) 306
SetI ASST 9 cut(s) 118, 154, 174, 196, 230, 380, 461, 520, 544
SfaNI GCATC 1 cut(s) 404
SfcI CTRYAG 1 cut(s) 444
SinI GGWCC 2 cut(s) 194, 209
SsiI CCGC 5 cut(s) 38, 81, 546, 552, 570
SspMI CTAG 1 cut(s) 317
StyD4I CCNGG 1 cut(s) 511
StyI CCWWGG 1 cut(s) 213
TaiI ACGT 1 cut(s) 118
TaqI TCGA 5 cut(s) 24, 75, 158, 243, 285
TfiI GAWTC 4 cut(s) 26, 155, 369, 597
Tru1I TTAA 3 cut(s) 201, 291, 327
Tru9I TTAA 3 cut(s) 201, 291, 327
TseI GCWGC 1 cut(s) 313
TspDTI ATGAA 1 cut(s) 518
TspGWI ACGGA 1 cut(s) 162
Van91I CCANNNNNTGG 1 cut(s) 61
VpaK11BI GGWCC 2 cut(s) 194, 209
XspI CTAG 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.