RchiOBHm_Chr7g0181461

Protein of unknown function (DUF3223)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0014579
Physical Location & Seq
Forward (+)
3109287 .. 3111319
2033 bp
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UTR
Exon/CDS
Intron
PRQ16184

Sequence Viewer

Length: 666 bp
ATGGCAGAGGCAACCGTCGCTGAAATCGCCGAGACCGAGACCGAGACCAACACCGCGGCGGCCGAGGACATGGACCTCGAAACCGCCCCGGCCCAAAACCCGGACGAGGCCCAGAACGGCGACGCAAACTCTAAGCGGGCCAGGGAAGAAGAAGAGAGCGGCGGCGACGGCGCGGCGAAGAAGACGAAGGTGGAGGAGGAGAAGTCAGCGGAAGAGCAACGATTGGAGAAGTTAGGGGAAGGAGAGAGCGAGGCGGGTCGGGTCATCTTGGGTCCGAAGAGTTTCGGGTCGGCCGTTGAGATGTTCGACTATTTCTATAAGCTACTCCATTACTGGCCAACCGATCTTAATATTAATAAGTATGAATATCTGGTTTTACTGGACTTGCTCAAGAAGGGTCACTCTGAGCCTGATAAAAAGATTGGTGGAGGAATCCAGGCTTTCCAAGTTCGGTTCCACCCATTGTATAGAAGTAGATGCTACTTCCTTGTTAGAGAAGATGGAGCCAGTGATGATTTTAGTTTCAGGAAGTGTGTGGATCATATACTTCCCTTGCCAGAGAACATGAAAGCACACGCTGATGCCAACAAAGCCTTAGGCGGTGGCAAGGGTGGTGGGGGGAGAGGAAGCTGGCGTGGCCGTGGAAGGGGCAGGTCAAGAAACTGA

Protein Analysis

221

Amino Acids

24.37

Weight (kDa)

5.31

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 107 - 179 2.3e-25 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62440
fragaria_vesca FvH4_5g14920
malus_domestica MD06G1193500.v1.1 MD14G1200600.v1.1
prunus_persica Prupe.5G193800_v2.0.a1
pyrus_communis pycom06g17160 pycom14g16630
rosa_chinensis RchiOBHm_Chr7g0181461
rosa_laevigata RLG00000005192
rosa_multiflora Rmu_sc0004621.1_g000022
rosa_roxburghii Rroxscaffold_3G00271980
rosa_rugosa Rorug06G0441500
rosa_samantha Rh7AG043300 Rh7BG042700 Rh7CG044500 Rh7DG043100
rosa_wichuraiana Rw7G003600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 642
AccBSI CCGCTC 1 cut(s) 159
AccII CGCG 2 cut(s) 56, 173
AclWI GGATC 1 cut(s) 546
AcoI YGGCCR 4 cut(s) 60, 291, 335, 637
AfiI CCNNNNNNNGG 3 cut(s) 100, 106, 646
AjnI CCWGG 2 cut(s) 140, 435
AluBI AGCT 2 cut(s) 322, 630
AluI AGCT 2 cut(s) 322, 630
Alw26I GTCTC 3 cut(s) 26, 32, 38
AlwI GGATC 1 cut(s) 546
AoxI GGCC 7 cut(s) 60, 90, 108, 138, 291, 335, 637
AseI ATTAAT 1 cut(s) 354
Asp700I GAANNNNTTC 1 cut(s) 281
AspLEI GCGC 1 cut(s) 173
AspS9I GGNCC 5 cut(s) 73, 91, 109, 138, 272
AsuC2I CCSGG 2 cut(s) 89, 101
AvaII GGWCC 2 cut(s) 73, 272
AxyI CCTNAGG 1 cut(s) 595
BalI TGGCCA 1 cut(s) 337
BbsI GAAGAC 1 cut(s) 188
BccI CCATC 1 cut(s) 494
BceAI ACGGC 4 cut(s) 133, 184, 278, 624
BciT130I CCWGG 2 cut(s) 142, 437
BcnI CCSGG 2 cut(s) 89, 101
BcoDI GTCTC 3 cut(s) 26, 32, 38
BfuAI ACCTGC 1 cut(s) 642
BisI GCNGC 5 cut(s) 57, 60, 160, 163, 174
BlsI GCNGC 5 cut(s) 58, 61, 161, 164, 175
Bme1390I CCNGG 4 cut(s) 89, 101, 142, 437
Bme18I GGWCC 2 cut(s) 73, 272
BmgT120I GGNCC 5 cut(s) 73, 91, 109, 138, 272
BmiI GGNNCC 3 cut(s) 273, 455, 505
BmrFI CCNGG 4 cut(s) 89, 101, 142, 437
BmsI GCATC 2 cut(s) 467, 571
BpiI GAAGAC 1 cut(s) 188
BpuEI CTTGAG 1 cut(s) 374
BpuMI CCSGG 2 cut(s) 89, 101
BsaI GGTCTC 3 cut(s) 26, 32, 38
BsaJI CCNNGG 5 cut(s) 54, 63, 87, 141, 640
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 106, 646
Bse1I ACTGG 3 cut(s) 338, 384, 507
Bse21I CCTNAGG 1 cut(s) 595
BseBI CCWGG 2 cut(s) 142, 437
BseDI CCNNGG 5 cut(s) 54, 63, 87, 141, 640
BseLI CCNNNNNNNGG 3 cut(s) 100, 106, 646
BseMII CTCAG 1 cut(s) 396
BseNI ACTGG 3 cut(s) 338, 384, 507
BseRI GAGGAG 2 cut(s) 209, 212
BseX3I CGGCCG 2 cut(s) 60, 291
Bsh1236I CGCG 2 cut(s) 56, 173
Bsh1285I CGRYCG 2 cut(s) 63, 294
BshFI GGCC 7 cut(s) 62, 92, 110, 140, 293, 337, 639
BsiEI CGRYCG 2 cut(s) 63, 294
BsiSI CCGG 2 cut(s) 89, 101
BslI CCNNNNNNNGG 3 cut(s) 100, 106, 646
BsmAI GTCTC 3 cut(s) 26, 32, 38
BsnI GGCC 7 cut(s) 62, 92, 110, 140, 293, 337, 639
Bso31I GGTCTC 3 cut(s) 26, 32, 38
Bsp143I GATC 2 cut(s) 343, 538
BspANI GGCC 7 cut(s) 62, 92, 110, 140, 293, 337, 639
BspCNI CTCAG 1 cut(s) 397
BspFNI CGCG 2 cut(s) 56, 173
BspLI GGNNCC 3 cut(s) 273, 455, 505
BspMI ACCTGC 1 cut(s) 642
BspPI GGATC 1 cut(s) 546
BspQI GCTCTTC 1 cut(s) 207
BspTNI GGTCTC 3 cut(s) 26, 32, 38
BsrBI CCGCTC 1 cut(s) 159
BsrI ACTGG 3 cut(s) 338, 384, 507
BssECI CCNNGG 5 cut(s) 54, 63, 87, 141, 640
BssMI GATC 2 cut(s) 343, 538
Bst2UI CCWGG 2 cut(s) 142, 437
Bst4CI ACNGT 1 cut(s) 16
Bst6I CTCTTC 3 cut(s) 147, 207, 272
BstC8I GCNNGC 2 cut(s) 138, 632
BstDEI CTNAG 3 cut(s) 132, 405, 595
BstDSI CCRYGG 2 cut(s) 54, 640
BstFNI CGCG 2 cut(s) 56, 173
BstHHI GCGC 1 cut(s) 173
BstKTI GATC 2 cut(s) 346, 541
BstMAI GTCTC 3 cut(s) 26, 32, 38
BstMBI GATC 2 cut(s) 343, 538
BstMCI CGRYCG 2 cut(s) 63, 294
BstMWI GCNNNNNNNGC 5 cut(s) 17, 26, 168, 590, 636
BstNI CCWGG 2 cut(s) 142, 437
BstSCI CCNGG 4 cut(s) 87, 99, 140, 435
BstUI CGCG 2 cut(s) 56, 173
BstV2I GAAGAC 1 cut(s) 188
BstZI CGGCCG 2 cut(s) 60, 291
Bsu36I CCTNAGG 1 cut(s) 595
BsuRI GGCC 7 cut(s) 62, 92, 110, 140, 293, 337, 639
BtgI CCRYGG 2 cut(s) 54, 640
BtsIMutI CAGTG 1 cut(s) 514
BveI ACCTGC 1 cut(s) 642
Cac8I GCNNGC 2 cut(s) 138, 632
CfoI GCGC 1 cut(s) 173
Cfr13I GGNCC 5 cut(s) 73, 91, 109, 138, 272
Cfr42I CCGCGG 1 cut(s) 57
CseI GACGC 1 cut(s) 131
CspCI CAANNNNNGTGG 2 cut(s) 595, 630
CviAII CATG 2 cut(s) 70, 565
DdeI CTNAG 3 cut(s) 132, 405, 595
DpnI GATC 2 cut(s) 345, 540
DpnII GATC 2 cut(s) 343, 538
EaeI YGGCCR 4 cut(s) 60, 291, 335, 637
EagI CGGCCG 2 cut(s) 60, 291
Eam1104I CTCTTC 3 cut(s) 147, 207, 272
EarI CTCTTC 3 cut(s) 147, 207, 272
EclXI CGGCCG 2 cut(s) 60, 291
Eco31I GGTCTC 3 cut(s) 26, 32, 38
Eco47I GGWCC 2 cut(s) 73, 272
Eco52I CGGCCG 2 cut(s) 60, 291
Eco81I CCTNAGG 1 cut(s) 595
EcoRII CCWGG 2 cut(s) 140, 435
FaeI CATG 2 cut(s) 73, 568
FaiI YATR 7 cut(s) 71, 318, 363, 468, 543, 545, 566
FatI CATG 2 cut(s) 69, 564
FauI CCCGC 2 cut(s) 129, 247
Fnu4HI GCNGC 5 cut(s) 57, 60, 160, 163, 174
Fsp4HI GCNGC 5 cut(s) 57, 60, 160, 163, 174
GlaI GCGC 1 cut(s) 172
GluI GCNGC 5 cut(s) 57, 60, 160, 163, 174
HaeIII GGCC 7 cut(s) 62, 92, 110, 140, 293, 337, 639
HapII CCGG 2 cut(s) 89, 101
HgaI GACGC 1 cut(s) 131
HhaI GCGC 1 cut(s) 173
Hin1II CATG 2 cut(s) 73, 568
Hin6I GCGC 1 cut(s) 171
HinP1I GCGC 1 cut(s) 171
HinfI GANTC 1 cut(s) 432
HpaII CCGG 2 cut(s) 89, 101
Hpy188I TCNGA 2 cut(s) 276, 406
Hpy188III TCNNGA 3 cut(s) 391, 526, 657
Hpy99I CGWCG 3 cut(s) 20, 125, 170
HpyAV CCTTC 4 cut(s) 181, 233, 388, 639
HpyCH4III ACNGT 1 cut(s) 16
HpyF10VI GCNNNNNNNGC 5 cut(s) 17, 26, 168, 590, 636
HpyF3I CTNAG 3 cut(s) 132, 405, 595
Hsp92II CATG 2 cut(s) 73, 568
HspAI GCGC 1 cut(s) 171
KspI CCGCGG 1 cut(s) 57
Kzo9I GATC 2 cut(s) 343, 538
LguI GCTCTTC 1 cut(s) 207
LmnI GCTCC 1 cut(s) 503
LweI GCATC 2 cut(s) 467, 571
MaeIII GTNAC 1 cut(s) 398
MalI GATC 2 cut(s) 345, 540
MbiI CCGCTC 1 cut(s) 159
MboI GATC 2 cut(s) 343, 538
MboII GAAGA 8 cut(s) 158, 161, 164, 190, 193, 224, 289, 509
MlsI TGGCCA 1 cut(s) 337
MluNI TGGCCA 1 cut(s) 337
MnlI CCTC 8 cut(s) 58, 86, 100, 187, 190, 244, 422, 617
Mox20I TGGCCA 1 cut(s) 337
MroXI GAANNNNTTC 1 cut(s) 281
MscI TGGCCA 1 cut(s) 337
MseI TTAA 2 cut(s) 348, 354
MslI CAYNNNNRTG 1 cut(s) 579
Msp20I TGGCCA 1 cut(s) 337
MspA1I CMGCKG 2 cut(s) 56, 209
MspI CCGG 2 cut(s) 89, 101
MspR9I CCNGG 4 cut(s) 89, 101, 142, 437
MvaI CCWGG 2 cut(s) 142, 437
MvnI CGCG 2 cut(s) 56, 173
MwoI GCNNNNNNNGC 5 cut(s) 17, 26, 168, 590, 636
NciI CCSGG 2 cut(s) 89, 101
NdeII GATC 2 cut(s) 343, 538
NlaIII CATG 2 cut(s) 73, 568
NlaIV GGNNCC 3 cut(s) 273, 455, 505
NmeAIII GCCGAG 2 cut(s) 55, 88
NmuCI GTSAC 1 cut(s) 398
PciSI GCTCTTC 1 cut(s) 207
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 291
PdmI GAANNNNTTC 1 cut(s) 281
PfeI GAWTC 1 cut(s) 432
PkrI GCNGC 5 cut(s) 58, 61, 161, 164, 175
PshBI ATTAAT 1 cut(s) 354
Psp6I CCWGG 2 cut(s) 140, 435
PspGI CCWGG 2 cut(s) 140, 435
PspN4I GGNNCC 3 cut(s) 273, 455, 505
PspPI GGNCC 5 cut(s) 73, 91, 109, 138, 272
RseI CAYNNNNRTG 1 cut(s) 579
SacII CCGCGG 1 cut(s) 57
SapI GCTCTTC 1 cut(s) 207
SaqAI TTAA 2 cut(s) 348, 354
SatI GCNGC 5 cut(s) 57, 60, 160, 163, 174
Sau3AI GATC 2 cut(s) 343, 538
Sau96I GGNCC 5 cut(s) 73, 91, 109, 138, 272
ScrFI CCNGG 4 cut(s) 89, 101, 142, 437
SetI ASST 5 cut(s) 78, 192, 324, 632, 656
SfaNI GCATC 2 cut(s) 467, 571
Sfr303I CCGCGG 1 cut(s) 57
SgrBI CCGCGG 1 cut(s) 57
SinI GGWCC 2 cut(s) 73, 272
SmiMI CAYNNNNRTG 1 cut(s) 579
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
SspI AATATT 1 cut(s) 352
StyD4I CCNGG 4 cut(s) 87, 99, 140, 435
TaaI ACNGT 1 cut(s) 16
TaqI TCGA 2 cut(s) 78, 306
TaqII GACCGA 2 cut(s) 50, 56
TauI GCSGC 5 cut(s) 59, 62, 162, 165, 176
TfiI GAWTC 1 cut(s) 432
Tru1I TTAA 2 cut(s) 348, 354
Tru9I TTAA 2 cut(s) 348, 354
TscAI CASTG 1 cut(s) 514
TseFI GTSAC 1 cut(s) 398
Tsp45I GTSAC 1 cut(s) 398
TspDTI ATGAA 2 cut(s) 378, 581
TspRI CASTG 1 cut(s) 514
VpaK11BI GGWCC 2 cut(s) 73, 272
VspI ATTAAT 1 cut(s) 354
XmnI GAANNNNTTC 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.