FvH4_5g14920

Protein of unknown function (DUF3223)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0014579
Physical Location & Seq
Reverse (-)
8441929 .. 8443946
2018 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14920.t1

Sequence Viewer

Length: 657 bp
ATGGCAGAGACCACCGTCGCTGAAATCGCCGATACCAACCCCGCGGCCGAGGACATGGACCTTGAAACCGCCCCTGCCCAAAATATCGACGAGGCCCAGAACGGCGATGCCACAGCTAAGCGGGCCCGGGAGGAAGAAGAGGCGGAGGAGAGCGGCGGCGACGGCGCGGCGAAGAAGGCGAAGGTGGAGGAGAAGTCAGCTGAGGAGCAAAGGTTGGAGAAGTTAGGGGAAGGAGAGAACGGGGCGGGTCGGGTCAGCTTGGGTCCGAAGAGTTTCGGTTCGGCGGTTGAGATGTTCGACTATTTCTATAAGCTCCTGCATTATTGGCCCACTGATCTTAATATCAATAAGTATGAATATCTGGTTTTGCTGGACTTGCTCAAGAAGGGTCACTCTGAGCCTGATAAAAAGATTGGTGGAGGGATCCAGGCTTTCCAAGTCCGGTTCCATCCATTGTATAGAAGTAGGTGCTACTTCCTTGTTAGGGAAGATGGAGCCAGTGATGATTTTAGTTTCAGAAAGTGTGTGGATCATATACTTCCCTTGCCAGAGAACATGAAAGCACACGGTGATGCGAACAAAGCCTTAGGCGGCGGCAAGGGTGGTGGGGGTAGAGGAAGTTGGCGTGGCCGTGGAAGGGGCAGGGGAAGAAACTGA

Protein Analysis

219

Amino Acids

23.89

Weight (kDa)

5.52

Isoelectric Point (pI)

37.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 104 - 176 2.3e-25 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62440
fragaria_vesca FvH4_5g14920
malus_domestica MD06G1193500.v1.1 MD14G1200600.v1.1
prunus_persica Prupe.5G193800_v2.0.a1
pyrus_communis pycom06g17160 pycom14g16630
rosa_chinensis RchiOBHm_Chr7g0181461
rosa_laevigata RLG00000005192
rosa_multiflora Rmu_sc0004621.1_g000022
rosa_roxburghii Rroxscaffold_3G00271980
rosa_rugosa Rorug06G0441500
rosa_samantha Rh7AG043300 Rh7BG042700 Rh7CG044500 Rh7DG043100
rosa_wichuraiana Rw7G003600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 153
AccII CGCG 2 cut(s) 44, 167
AclWI GGATC 3 cut(s) 418, 431, 537
AcoI YGGCCR 2 cut(s) 45, 628
AdeI CACNNNGTG 1 cut(s) 569
AfiI CCNNNNNNNGG 2 cut(s) 484, 637
AgsI TTSAA 1 cut(s) 65
AjnI CCWGG 1 cut(s) 426
AluBI AGCT 4 cut(s) 116, 200, 258, 313
AluI AGCT 4 cut(s) 116, 200, 258, 313
Alw26I GTCTC 1 cut(s) 2
AlwI GGATC 3 cut(s) 418, 431, 537
Ama87I CYCGRG 1 cut(s) 126
AoxI GGCC 5 cut(s) 45, 93, 123, 326, 628
ApaI GGGCCC 1 cut(s) 127
Asp700I GAANNNNTTC 1 cut(s) 272
AspLEI GCGC 1 cut(s) 167
AspS9I GGNCC 6 cut(s) 58, 94, 123, 124, 263, 327
AsuC2I CCSGG 2 cut(s) 127, 128
AsuHPI GGTGA 1 cut(s) 581
AvaI CYCGRG 1 cut(s) 126
AvaII GGWCC 2 cut(s) 58, 263
AxyI CCTNAGG 1 cut(s) 586
BaeGI GKGCMC 1 cut(s) 127
BamHI GGATCC 1 cut(s) 423
BanII GRGCYC 1 cut(s) 127
BbvCI CCTCAGC 1 cut(s) 201
BccI CCATC 2 cut(s) 456, 485
BceAI ACGGC 3 cut(s) 118, 178, 615
BciT130I CCWGG 1 cut(s) 428
BcnI CCSGG 2 cut(s) 127, 128
BcoDI GTCTC 1 cut(s) 2
BisI GCNGC 6 cut(s) 45, 154, 157, 168, 592, 595
BlpI GCTNAGC 1 cut(s) 117
BlsI GCNGC 6 cut(s) 46, 155, 158, 169, 593, 596
Bme1390I CCNGG 3 cut(s) 127, 128, 428
Bme18I GGWCC 2 cut(s) 58, 263
BmeT110I CYCGRG 1 cut(s) 126
BmgT120I GGNCC 6 cut(s) 58, 94, 123, 124, 263, 327
BmiI GGNNCC 5 cut(s) 125, 264, 425, 446, 496
BmrFI CCNGG 3 cut(s) 127, 128, 428
BmsI GCATC 2 cut(s) 97, 562
BoxI GACNNNNGTC 1 cut(s) 14
Bpu10I CCTNAGC 1 cut(s) 201
Bpu1102I GCTNAGC 1 cut(s) 117
BpuEI CTTGAG 1 cut(s) 365
BpuMI CCSGG 2 cut(s) 127, 128
BsaI GGTCTC 1 cut(s) 2
BsaJI CCNNGG 4 cut(s) 42, 48, 126, 631
BsaWI WCCGGW 1 cut(s) 441
BsaXI ACNNNNNCTCC 4 cut(s) 179, 197, 209, 227
Bsc4I CCNNNNNNNGG 2 cut(s) 484, 637
Bse1I ACTGG 1 cut(s) 498
Bse21I CCTNAGG 1 cut(s) 586
BseBI CCWGG 1 cut(s) 428
BseDI CCNNGG 4 cut(s) 42, 48, 126, 631
BseGI GGATG 1 cut(s) 448
BseLI CCNNNNNNNGG 2 cut(s) 484, 637
BseMII CTCAG 2 cut(s) 192, 387
BseNI ACTGG 1 cut(s) 498
BseRI GAGGAG 3 cut(s) 161, 203, 218
BseSI GKGCMC 1 cut(s) 127
BseX3I CGGCCG 1 cut(s) 45
Bsh1236I CGCG 2 cut(s) 44, 167
Bsh1285I CGRYCG 1 cut(s) 48
BshFI GGCC 5 cut(s) 47, 95, 125, 328, 630
BsiEI CGRYCG 1 cut(s) 48
BsiHKCI CYCGRG 1 cut(s) 126
BsiSI CCGG 2 cut(s) 127, 442
BslI CCNNNNNNNGG 2 cut(s) 484, 637
BsmAI GTCTC 1 cut(s) 2
BsnI GGCC 5 cut(s) 47, 95, 125, 328, 630
Bso31I GGTCTC 1 cut(s) 2
BsoBI CYCGRG 1 cut(s) 126
Bsp120I GGGCCC 1 cut(s) 123
Bsp1286I GDGCHC 1 cut(s) 127
Bsp143I GATC 3 cut(s) 334, 423, 529
Bsp1720I GCTNAGC 1 cut(s) 117
BspANI GGCC 5 cut(s) 47, 95, 125, 328, 630
BspCNI CTCAG 2 cut(s) 193, 388
BspFNI CGCG 2 cut(s) 44, 167
BspLI GGNNCC 5 cut(s) 125, 264, 425, 446, 496
BspPI GGATC 3 cut(s) 418, 431, 537
BspTNI GGTCTC 1 cut(s) 2
BsrBI CCGCTC 1 cut(s) 153
BsrI ACTGG 1 cut(s) 498
BssECI CCNNGG 4 cut(s) 42, 48, 126, 631
BssMI GATC 3 cut(s) 334, 423, 529
Bst2UI CCWGG 1 cut(s) 428
Bst4CI ACNGT 2 cut(s) 16, 569
Bst6I CTCTTC 2 cut(s) 132, 263
BstC8I GCNNGC 1 cut(s) 123
BstDEI CTNAG 4 cut(s) 117, 201, 396, 586
BstDSI CCRYGG 2 cut(s) 42, 631
BstF5I GGATG 1 cut(s) 448
BstFNI CGCG 2 cut(s) 44, 167
BstHHI GCGC 1 cut(s) 167
BstKTI GATC 3 cut(s) 337, 426, 532
BstMAI GTCTC 1 cut(s) 2
BstMBI GATC 3 cut(s) 334, 423, 529
BstMCI CGRYCG 1 cut(s) 48
BstMWI GCNNNNNNNGC 7 cut(s) 26, 122, 162, 176, 325, 376, 581
BstNI CCWGG 1 cut(s) 428
BstPAI GACNNNNGTC 1 cut(s) 14
BstSCI CCNGG 3 cut(s) 125, 126, 426
BstSLI GKGCMC 1 cut(s) 127
BstUI CGCG 2 cut(s) 44, 167
BstX2I RGATCY 1 cut(s) 423
BstYI RGATCY 1 cut(s) 423
BstZI CGGCCG 1 cut(s) 45
Bsu36I CCTNAGG 1 cut(s) 586
BsuRI GGCC 5 cut(s) 47, 95, 125, 328, 630
BtgI CCRYGG 2 cut(s) 42, 631
BtgZI GCGATG 1 cut(s) 120
BtsCI GGATG 1 cut(s) 448
BtsIMutI CAGTG 2 cut(s) 330, 505
Cac8I GCNNGC 1 cut(s) 123
CfoI GCGC 1 cut(s) 167
Cfr13I GGNCC 6 cut(s) 58, 94, 123, 124, 263, 327
Cfr42I CCGCGG 1 cut(s) 45
Cfr9I CCCGGG 1 cut(s) 126
CspCI CAANNNNNGTGG 2 cut(s) 586, 621
CviAII CATG 2 cut(s) 55, 556
DdeI CTNAG 4 cut(s) 117, 201, 396, 586
DpnI GATC 3 cut(s) 336, 425, 531
DpnII GATC 3 cut(s) 334, 423, 529
DraIII CACNNNGTG 1 cut(s) 569
EaeI YGGCCR 2 cut(s) 45, 628
EagI CGGCCG 1 cut(s) 45
Eam1104I CTCTTC 2 cut(s) 132, 263
EarI CTCTTC 2 cut(s) 132, 263
EciI GGCGGA 1 cut(s) 158
EclXI CGGCCG 1 cut(s) 45
Eco24I GRGCYC 1 cut(s) 127
Eco31I GGTCTC 1 cut(s) 2
Eco47I GGWCC 2 cut(s) 58, 263
Eco52I CGGCCG 1 cut(s) 45
Eco81I CCTNAGG 1 cut(s) 586
Eco88I CYCGRG 1 cut(s) 126
EcoRII CCWGG 1 cut(s) 426
EcoT38I GRGCYC 1 cut(s) 127
FaeI CATG 2 cut(s) 58, 559
FaiI YATR 7 cut(s) 56, 309, 354, 459, 534, 536, 557
FatI CATG 2 cut(s) 54, 555
FauI CCCGC 3 cut(s) 49, 114, 238
Fnu4HI GCNGC 6 cut(s) 45, 154, 157, 168, 592, 595
FokI GGATG 1 cut(s) 435
FriOI GRGCYC 1 cut(s) 127
Fsp4HI GCNGC 6 cut(s) 45, 154, 157, 168, 592, 595
GlaI GCGC 1 cut(s) 166
GluI GCNGC 6 cut(s) 45, 154, 157, 168, 592, 595
HaeIII GGCC 5 cut(s) 47, 95, 125, 328, 630
HapII CCGG 2 cut(s) 127, 442
HhaI GCGC 1 cut(s) 167
Hin1II CATG 2 cut(s) 58, 559
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HpaII CCGG 2 cut(s) 127, 442
HphI GGTGA 1 cut(s) 581
Hpy188I TCNGA 3 cut(s) 267, 397, 518
Hpy188III TCNNGA 1 cut(s) 382
Hpy99I CGWCG 3 cut(s) 20, 92, 164
HpyAV CCTTC 5 cut(s) 169, 175, 224, 379, 630
HpyCH4III ACNGT 2 cut(s) 16, 569
HpyCH4V TGCA 1 cut(s) 319
HpyF10VI GCNNNNNNNGC 7 cut(s) 26, 122, 162, 176, 325, 376, 581
HpyF3I CTNAG 4 cut(s) 117, 201, 396, 586
Hsp92II CATG 2 cut(s) 58, 559
HspAI GCGC 1 cut(s) 165
KspI CCGCGG 1 cut(s) 45
Kzo9I GATC 3 cut(s) 334, 423, 529
LmnI GCTCC 3 cut(s) 205, 318, 494
LweI GCATC 2 cut(s) 97, 562
MaeIII GTNAC 1 cut(s) 389
MalI GATC 3 cut(s) 336, 425, 531
MbiI CCGCTC 1 cut(s) 153
MboI GATC 3 cut(s) 334, 423, 529
MboII GAAGA 5 cut(s) 146, 149, 184, 280, 500
MflI RGATCY 1 cut(s) 423
MhlI GDGCHC 1 cut(s) 127
MmeI TCCRAC 1 cut(s) 195
MnlI CCTC 9 cut(s) 43, 85, 124, 133, 139, 181, 196, 413, 608
MroXI GAANNNNTTC 1 cut(s) 272
MseI TTAA 1 cut(s) 339
MslI CAYNNNNRTG 1 cut(s) 570
MspA1I CMGCKG 2 cut(s) 44, 200
MspI CCGG 2 cut(s) 127, 442
MspR9I CCNGG 3 cut(s) 127, 128, 428
MvaI CCWGG 1 cut(s) 428
MvnI CGCG 2 cut(s) 44, 167
MwoI GCNNNNNNNGC 7 cut(s) 26, 122, 162, 176, 325, 376, 581
NciI CCSGG 2 cut(s) 127, 128
NdeII GATC 3 cut(s) 334, 423, 529
NlaIII CATG 2 cut(s) 58, 559
NlaIV GGNNCC 5 cut(s) 125, 264, 425, 446, 496
NmeAIII GCCGAG 1 cut(s) 73
NmuCI GTSAC 1 cut(s) 389
PdmI GAANNNNTTC 1 cut(s) 272
PkrI GCNGC 6 cut(s) 46, 155, 158, 169, 593, 596
PshAI GACNNNNGTC 1 cut(s) 14
Psp6I CCWGG 1 cut(s) 426
PspGI CCWGG 1 cut(s) 426
PspN4I GGNNCC 5 cut(s) 125, 264, 425, 446, 496
PspOMI GGGCCC 1 cut(s) 123
PspPI GGNCC 6 cut(s) 58, 94, 123, 124, 263, 327
PsuI RGATCY 1 cut(s) 423
PvuII CAGCTG 1 cut(s) 200
RseI CAYNNNNRTG 1 cut(s) 570
SacII CCGCGG 1 cut(s) 45
SaqAI TTAA 1 cut(s) 339
SatI GCNGC 6 cut(s) 45, 154, 157, 168, 592, 595
Sau3AI GATC 3 cut(s) 334, 423, 529
Sau96I GGNCC 6 cut(s) 58, 94, 123, 124, 263, 327
ScrFI CCNGG 3 cut(s) 127, 128, 428
SduI GDGCHC 1 cut(s) 127
SetI ASST 8 cut(s) 63, 118, 186, 202, 215, 260, 315, 470
SfaNI GCATC 2 cut(s) 97, 562
Sfr303I CCGCGG 1 cut(s) 45
SgrBI CCGCGG 1 cut(s) 45
SinI GGWCC 2 cut(s) 58, 263
SmaI CCCGGG 1 cut(s) 128
SmiMI CAYNNNNRTG 1 cut(s) 570
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
StyD4I CCNGG 3 cut(s) 125, 126, 426
TaaI ACNGT 2 cut(s) 16, 569
TaqI TCGA 2 cut(s) 87, 297
TauI GCSGC 6 cut(s) 47, 156, 159, 170, 594, 597
Tru1I TTAA 1 cut(s) 339
Tru9I TTAA 1 cut(s) 339
TscAI CASTG 2 cut(s) 337, 505
TseFI GTSAC 1 cut(s) 389
Tsp45I GTSAC 1 cut(s) 389
TspDTI ATGAA 2 cut(s) 369, 572
TspMI CCCGGG 1 cut(s) 126
TspRI CASTG 2 cut(s) 337, 505
VpaK11BI GGWCC 2 cut(s) 58, 263
XmaI CCCGGG 1 cut(s) 126
XmnI GAANNNNTTC 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.