Rh7CG044500

Protein of unknown function (DUF3223)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0014579
Physical Location & Seq
Forward (+)
2999556 .. 3001828
2273 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG044500.1

Sequence Viewer

Length: 669 bp
ATGGCAGAGGCAACCGTCGCTGAAATCGCCGAGACCGAGACCAACACCAACACCGCGGCGGCCGAGGACATGGACCTCGAAACCGCCCCGGCCCAAAACCCAGACGAGGCCCAGAATGGCGACGCAAACTCTAAGCGGGCCAGGGAAGAAGAAGAAGAGAGCGGCGGCGACGGCGCGGCGAAGAAGACGAAGGTGGAGGAGGAGAAGTCAGCGGAAGAGCAACGATTGGAGAAGTTAGGGGAAGGAGAGAGCGAGCCGGGTCGGGTCATCTTGGGTCCGAAGAGTTTCGGGTCGGCCGTTGAGATGTTCGACTATTTCTATAAGCTACTCCATTACTGGCCAACCGATCTTAATATTAATAAGTATGAATATCTGGTTTTGCTGGACTTGCTCAAGAAGGGTCACTCTGAGCCTGATAAAAAGATTGGTGGAGGAATCCAGGCTTTCCAAGTTCGGTTCCACCCATTGTATAGAAGTAGATGCTACTTCCTTGTTAGAGAAGATGGAGCCAGTGATGATTTTAGTTTCAGGAAGTGTGTGGATCATATACTTCCCTTGCCAGAGAACATGAAAGCACACGCTGATGCCAACAAAGCCTTAGGCGGTGGCAAGGGTGGTGGGGGGAGAGGAAGCTGGCGTGGCCGTGGAAGGGGCAGGTCAAGAAACTGA

Protein Analysis

222

Amino Acids

24.51

Weight (kDa)

5.31

Isoelectric Point (pI)

44.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 108 - 180 2.4e-25 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62440
fragaria_vesca FvH4_5g14920
malus_domestica MD06G1193500.v1.1 MD14G1200600.v1.1
prunus_persica Prupe.5G193800_v2.0.a1
pyrus_communis pycom06g17160 pycom14g16630
rosa_chinensis RchiOBHm_Chr7g0181461
rosa_laevigata RLG00000005192
rosa_multiflora Rmu_sc0004621.1_g000022
rosa_roxburghii Rroxscaffold_3G00271980
rosa_rugosa Rorug06G0441500
rosa_samantha Rh7AG043300 Rh7BG042700 Rh7CG044500 Rh7DG043100
rosa_wichuraiana Rw7G003600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 645
AccBSI CCGCTC 1 cut(s) 162
AccII CGCG 2 cut(s) 56, 176
AclWI GGATC 1 cut(s) 549
AcoI YGGCCR 4 cut(s) 60, 294, 338, 640
AfiI CCNNNNNNNGG 2 cut(s) 106, 649
AjnI CCWGG 2 cut(s) 140, 438
AluBI AGCT 2 cut(s) 325, 633
AluI AGCT 2 cut(s) 325, 633
Alw26I GTCTC 2 cut(s) 26, 32
AlwI GGATC 1 cut(s) 549
AoxI GGCC 7 cut(s) 60, 90, 108, 138, 294, 338, 640
AseI ATTAAT 1 cut(s) 357
Asp700I GAANNNNTTC 1 cut(s) 284
AspLEI GCGC 1 cut(s) 176
AspS9I GGNCC 5 cut(s) 73, 91, 109, 138, 275
AsuC2I CCSGG 2 cut(s) 89, 258
AvaII GGWCC 2 cut(s) 73, 275
AxyI CCTNAGG 1 cut(s) 598
BalI TGGCCA 1 cut(s) 340
BbsI GAAGAC 1 cut(s) 191
BccI CCATC 1 cut(s) 497
BceAI ACGGC 3 cut(s) 187, 281, 627
BciT130I CCWGG 2 cut(s) 142, 440
BcnI CCSGG 2 cut(s) 89, 258
BcoDI GTCTC 2 cut(s) 26, 32
BfuAI ACCTGC 1 cut(s) 645
BisI GCNGC 5 cut(s) 57, 60, 163, 166, 177
BlsI GCNGC 5 cut(s) 58, 61, 164, 167, 178
Bme1390I CCNGG 4 cut(s) 89, 142, 258, 440
Bme18I GGWCC 2 cut(s) 73, 275
BmgT120I GGNCC 5 cut(s) 73, 91, 109, 138, 275
BmiI GGNNCC 3 cut(s) 276, 458, 508
BmrFI CCNGG 4 cut(s) 89, 142, 258, 440
BmsI GCATC 2 cut(s) 470, 574
BpiI GAAGAC 1 cut(s) 191
BpuEI CTTGAG 1 cut(s) 377
BpuMI CCSGG 2 cut(s) 89, 258
BsaI GGTCTC 2 cut(s) 26, 32
BsaJI CCNNGG 5 cut(s) 54, 63, 87, 141, 643
BsaXI ACNNNNNCTCC 2 cut(s) 191, 221
Bsc4I CCNNNNNNNGG 2 cut(s) 106, 649
Bse1I ACTGG 2 cut(s) 341, 510
Bse21I CCTNAGG 1 cut(s) 598
BseBI CCWGG 2 cut(s) 142, 440
BseDI CCNNGG 5 cut(s) 54, 63, 87, 141, 643
BseLI CCNNNNNNNGG 2 cut(s) 106, 649
BseMII CTCAG 1 cut(s) 399
BseNI ACTGG 2 cut(s) 341, 510
BseRI GAGGAG 2 cut(s) 212, 215
BseX3I CGGCCG 2 cut(s) 60, 294
Bsh1236I CGCG 2 cut(s) 56, 176
Bsh1285I CGRYCG 2 cut(s) 63, 297
BshFI GGCC 7 cut(s) 62, 92, 110, 140, 296, 340, 642
BsiEI CGRYCG 2 cut(s) 63, 297
BsiSI CCGG 2 cut(s) 89, 257
BslI CCNNNNNNNGG 2 cut(s) 106, 649
BsmAI GTCTC 2 cut(s) 26, 32
BsnI GGCC 7 cut(s) 62, 92, 110, 140, 296, 340, 642
Bso31I GGTCTC 2 cut(s) 26, 32
Bsp143I GATC 2 cut(s) 346, 541
BspANI GGCC 7 cut(s) 62, 92, 110, 140, 296, 340, 642
BspCNI CTCAG 1 cut(s) 400
BspFNI CGCG 2 cut(s) 56, 176
BspLI GGNNCC 3 cut(s) 276, 458, 508
BspMI ACCTGC 1 cut(s) 645
BspPI GGATC 1 cut(s) 549
BspQI GCTCTTC 1 cut(s) 210
BspTNI GGTCTC 2 cut(s) 26, 32
BsrBI CCGCTC 1 cut(s) 162
BsrI ACTGG 2 cut(s) 341, 510
BssECI CCNNGG 5 cut(s) 54, 63, 87, 141, 643
BssMI GATC 2 cut(s) 346, 541
Bst2UI CCWGG 2 cut(s) 142, 440
Bst4CI ACNGT 1 cut(s) 16
Bst6I CTCTTC 3 cut(s) 150, 210, 275
BstC8I GCNNGC 3 cut(s) 138, 254, 635
BstDEI CTNAG 3 cut(s) 132, 408, 598
BstDSI CCRYGG 2 cut(s) 54, 643
BstFNI CGCG 2 cut(s) 56, 176
BstHHI GCGC 1 cut(s) 176
BstKTI GATC 2 cut(s) 349, 544
BstMAI GTCTC 2 cut(s) 26, 32
BstMBI GATC 2 cut(s) 346, 541
BstMCI CGRYCG 2 cut(s) 63, 297
BstMWI GCNNNNNNNGC 6 cut(s) 17, 26, 171, 388, 593, 639
BstNI CCWGG 2 cut(s) 142, 440
BstSCI CCNGG 4 cut(s) 87, 140, 256, 438
BstUI CGCG 2 cut(s) 56, 176
BstV2I GAAGAC 1 cut(s) 191
BstZI CGGCCG 2 cut(s) 60, 294
Bsu36I CCTNAGG 1 cut(s) 598
BsuRI GGCC 7 cut(s) 62, 92, 110, 140, 296, 340, 642
BtgI CCRYGG 2 cut(s) 54, 643
BtsIMutI CAGTG 1 cut(s) 517
BveI ACCTGC 1 cut(s) 645
Cac8I GCNNGC 3 cut(s) 138, 254, 635
CfoI GCGC 1 cut(s) 176
Cfr13I GGNCC 5 cut(s) 73, 91, 109, 138, 275
Cfr42I CCGCGG 1 cut(s) 57
CseI GACGC 1 cut(s) 131
CspCI CAANNNNNGTGG 2 cut(s) 598, 633
CviAII CATG 2 cut(s) 70, 568
DdeI CTNAG 3 cut(s) 132, 408, 598
DpnI GATC 2 cut(s) 348, 543
DpnII GATC 2 cut(s) 346, 541
EaeI YGGCCR 4 cut(s) 60, 294, 338, 640
EagI CGGCCG 2 cut(s) 60, 294
Eam1104I CTCTTC 3 cut(s) 150, 210, 275
EarI CTCTTC 3 cut(s) 150, 210, 275
EclXI CGGCCG 2 cut(s) 60, 294
Eco31I GGTCTC 2 cut(s) 26, 32
Eco47I GGWCC 2 cut(s) 73, 275
Eco52I CGGCCG 2 cut(s) 60, 294
Eco81I CCTNAGG 1 cut(s) 598
EcoRII CCWGG 2 cut(s) 140, 438
FaeI CATG 2 cut(s) 73, 571
FaiI YATR 7 cut(s) 71, 321, 366, 471, 546, 548, 569
FatI CATG 2 cut(s) 69, 567
FauI CCCGC 1 cut(s) 129
Fnu4HI GCNGC 5 cut(s) 57, 60, 163, 166, 177
Fsp4HI GCNGC 5 cut(s) 57, 60, 163, 166, 177
GlaI GCGC 1 cut(s) 175
GluI GCNGC 5 cut(s) 57, 60, 163, 166, 177
HaeIII GGCC 7 cut(s) 62, 92, 110, 140, 296, 340, 642
HapII CCGG 2 cut(s) 89, 257
HgaI GACGC 1 cut(s) 131
HhaI GCGC 1 cut(s) 176
Hin1II CATG 2 cut(s) 73, 571
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HinfI GANTC 1 cut(s) 435
HpaII CCGG 2 cut(s) 89, 257
Hpy188I TCNGA 2 cut(s) 279, 409
Hpy188III TCNNGA 3 cut(s) 394, 529, 660
Hpy99I CGWCG 3 cut(s) 20, 125, 173
HpyAV CCTTC 4 cut(s) 184, 236, 391, 642
HpyCH4III ACNGT 1 cut(s) 16
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 26, 171, 388, 593, 639
HpyF3I CTNAG 3 cut(s) 132, 408, 598
Hsp92II CATG 2 cut(s) 73, 571
HspAI GCGC 1 cut(s) 174
KspI CCGCGG 1 cut(s) 57
Kzo9I GATC 2 cut(s) 346, 541
LguI GCTCTTC 1 cut(s) 210
LmnI GCTCC 1 cut(s) 506
LweI GCATC 2 cut(s) 470, 574
MaeIII GTNAC 1 cut(s) 401
MalI GATC 2 cut(s) 348, 543
MbiI CCGCTC 1 cut(s) 162
MboI GATC 2 cut(s) 346, 541
MboII GAAGA 9 cut(s) 158, 161, 164, 167, 193, 196, 227, 292, 512
MlsI TGGCCA 1 cut(s) 340
MluNI TGGCCA 1 cut(s) 340
MnlI CCTC 7 cut(s) 58, 86, 100, 190, 193, 425, 620
Mox20I TGGCCA 1 cut(s) 340
MroXI GAANNNNTTC 1 cut(s) 284
MscI TGGCCA 1 cut(s) 340
MseI TTAA 2 cut(s) 351, 357
MslI CAYNNNNRTG 1 cut(s) 582
Msp20I TGGCCA 1 cut(s) 340
MspA1I CMGCKG 2 cut(s) 56, 212
MspI CCGG 2 cut(s) 89, 257
MspR9I CCNGG 4 cut(s) 89, 142, 258, 440
MvaI CCWGG 2 cut(s) 142, 440
MvnI CGCG 2 cut(s) 56, 176
MwoI GCNNNNNNNGC 6 cut(s) 17, 26, 171, 388, 593, 639
NciI CCSGG 2 cut(s) 89, 258
NdeII GATC 2 cut(s) 346, 541
NlaIII CATG 2 cut(s) 73, 571
NlaIV GGNNCC 3 cut(s) 276, 458, 508
NmeAIII GCCGAG 2 cut(s) 55, 88
NmuCI GTSAC 1 cut(s) 401
PciSI GCTCTTC 1 cut(s) 210
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 294
PdmI GAANNNNTTC 1 cut(s) 284
PfeI GAWTC 1 cut(s) 435
PkrI GCNGC 5 cut(s) 58, 61, 164, 167, 178
PshBI ATTAAT 1 cut(s) 357
Psp6I CCWGG 2 cut(s) 140, 438
PspGI CCWGG 2 cut(s) 140, 438
PspN4I GGNNCC 3 cut(s) 276, 458, 508
PspPI GGNCC 5 cut(s) 73, 91, 109, 138, 275
RseI CAYNNNNRTG 1 cut(s) 582
SacII CCGCGG 1 cut(s) 57
SapI GCTCTTC 1 cut(s) 210
SaqAI TTAA 2 cut(s) 351, 357
SatI GCNGC 5 cut(s) 57, 60, 163, 166, 177
Sau3AI GATC 2 cut(s) 346, 541
Sau96I GGNCC 5 cut(s) 73, 91, 109, 138, 275
ScrFI CCNGG 4 cut(s) 89, 142, 258, 440
SetI ASST 5 cut(s) 78, 195, 327, 635, 659
SfaNI GCATC 2 cut(s) 470, 574
Sfr303I CCGCGG 1 cut(s) 57
SgrBI CCGCGG 1 cut(s) 57
SinI GGWCC 2 cut(s) 73, 275
SmiMI CAYNNNNRTG 1 cut(s) 582
SmlI CTYRAG 1 cut(s) 392
SmoI CTYRAG 1 cut(s) 392
SspI AATATT 1 cut(s) 355
StyD4I CCNGG 4 cut(s) 87, 140, 256, 438
TaaI ACNGT 1 cut(s) 16
TaqI TCGA 2 cut(s) 78, 309
TaqII GACCGA 1 cut(s) 50
TauI GCSGC 5 cut(s) 59, 62, 165, 168, 179
TfiI GAWTC 1 cut(s) 435
Tru1I TTAA 2 cut(s) 351, 357
Tru9I TTAA 2 cut(s) 351, 357
TscAI CASTG 1 cut(s) 517
TseFI GTSAC 1 cut(s) 401
Tsp45I GTSAC 1 cut(s) 401
TspDTI ATGAA 2 cut(s) 381, 584
TspRI CASTG 1 cut(s) 517
VpaK11BI GGWCC 2 cut(s) 73, 275
VspI ATTAAT 1 cut(s) 357
XmnI GAANNNNTTC 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.