Rh7AG043300

Protein of unknown function (DUF3223)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0014579
Physical Location & Seq
Forward (+)
2962771 .. 2965097
2327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG043300.1

Sequence Viewer

Length: 675 bp
ATGGCAGAGGCAACCGTCGCTGAAATCGCCGAGACCGAGACCACCAACACCGCGGCGGCCGAGGACATGGACCTCGAAACCGCCCCGGGCCAAAACCCAGACGAGGCCCAGAACGGCGACGCAAACTCTAAGCGGGCCAGGGAAGAAGAAGAAGAAGAAGAAGAGAGCGGCGGCGACAGCGCGGCGAAGAAGACGAAGGTGGAGGAGGAGAAGTCAGCGGAAGAGCAACGATTGGAAAAGTTAGGGGAAGGAGAGAGCGAGGCGGGTCGGGTCAGCTTGGGTCCGAAGAGTTTCGGGTCGGCCGTTGAGATGTTCGACTATTTCTATAAGCTACTCCATTGCTGGCCAACCGATCTTAATATTAATAAGTATGAATATCTGGTTTTGCTGGACTTGCTCAAGAAGGGTCACTCTGAGCCTGATAAAAAGATTGGTGGAGGAATCCAGGCTTTCCAAGTTCGGTTCCACCCATTGTATAGAAGTAGATGCTACTTCCTTGTTAGAGAAGATGGAGCCAGTGATGATTTTAGTTTCAGGAAGTGTGTGGATCATATACTTCCCTTGCCAGAGAACATGAAAGCACACGCTGATGCCAACAAAGCCTTAGGCGGTGGCAAGGGTGGTGGGGGGAGAGGAAGCTGGCGTGGCCGTGGAAGGGGCAGGTCAAGAAACTGA

Protein Analysis

224

Amino Acids

24.69

Weight (kDa)

5.09

Isoelectric Point (pI)

47.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 110 - 182 5.3e-25 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0014579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62440
fragaria_vesca FvH4_5g14920
malus_domestica MD06G1193500.v1.1 MD14G1200600.v1.1
prunus_persica Prupe.5G193800_v2.0.a1
pyrus_communis pycom06g17160 pycom14g16630
rosa_chinensis RchiOBHm_Chr7g0181461
rosa_laevigata RLG00000005192
rosa_multiflora Rmu_sc0004621.1_g000022
rosa_roxburghii Rroxscaffold_3G00271980
rosa_rugosa Rorug06G0441500
rosa_samantha Rh7AG043300 Rh7BG042700 Rh7CG044500 Rh7DG043100
rosa_wichuraiana Rw7G003600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 651
AccBSI CCGCTC 1 cut(s) 168
AccII CGCG 2 cut(s) 53, 182
AclWI GGATC 1 cut(s) 555
AcoI YGGCCR 4 cut(s) 57, 300, 344, 646
AfiI CCNNNNNNNGG 2 cut(s) 103, 655
AjnI CCWGG 2 cut(s) 137, 444
AluBI AGCT 3 cut(s) 276, 331, 639
AluI AGCT 3 cut(s) 276, 331, 639
Alw26I GTCTC 2 cut(s) 26, 32
AlwI GGATC 1 cut(s) 555
Ama87I CYCGRG 1 cut(s) 85
AoxI GGCC 7 cut(s) 57, 88, 105, 135, 300, 344, 646
AseI ATTAAT 1 cut(s) 363
Asp700I GAANNNNTTC 1 cut(s) 290
AspLEI GCGC 1 cut(s) 182
AspS9I GGNCC 5 cut(s) 70, 88, 106, 135, 281
AsuC2I CCSGG 2 cut(s) 86, 87
AvaI CYCGRG 1 cut(s) 85
AvaII GGWCC 2 cut(s) 70, 281
AxyI CCTNAGG 1 cut(s) 604
BalI TGGCCA 1 cut(s) 346
BbsI GAAGAC 1 cut(s) 197
BccI CCATC 1 cut(s) 503
BceAI ACGGC 3 cut(s) 130, 287, 633
BciT130I CCWGG 2 cut(s) 139, 446
BcnI CCSGG 2 cut(s) 86, 87
BcoDI GTCTC 2 cut(s) 26, 32
BfuAI ACCTGC 1 cut(s) 651
BisI GCNGC 5 cut(s) 54, 57, 169, 172, 183
BlsI GCNGC 5 cut(s) 55, 58, 170, 173, 184
Bme1390I CCNGG 4 cut(s) 86, 87, 139, 446
Bme18I GGWCC 2 cut(s) 70, 281
BmeT110I CYCGRG 1 cut(s) 85
BmgT120I GGNCC 5 cut(s) 70, 88, 106, 135, 281
BmiI GGNNCC 3 cut(s) 282, 464, 514
BmrFI CCNGG 4 cut(s) 86, 87, 139, 446
BmsI GCATC 2 cut(s) 476, 580
BpiI GAAGAC 1 cut(s) 197
BpuEI CTTGAG 1 cut(s) 383
BpuMI CCSGG 2 cut(s) 86, 87
BsaI GGTCTC 2 cut(s) 26, 32
BsaJI CCNNGG 6 cut(s) 51, 60, 84, 85, 138, 649
BsaXI ACNNNNNCTCC 2 cut(s) 197, 227
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 655
Bse1I ACTGG 1 cut(s) 516
Bse21I CCTNAGG 1 cut(s) 604
Bse3DI GCAATG 1 cut(s) 337
BseBI CCWGG 2 cut(s) 139, 446
BseDI CCNNGG 6 cut(s) 51, 60, 84, 85, 138, 649
BseLI CCNNNNNNNGG 2 cut(s) 103, 655
BseMI GCAATG 1 cut(s) 337
BseMII CTCAG 1 cut(s) 405
BseNI ACTGG 1 cut(s) 516
BseRI GAGGAG 2 cut(s) 218, 221
BseX3I CGGCCG 2 cut(s) 57, 300
Bsh1236I CGCG 2 cut(s) 53, 182
Bsh1285I CGRYCG 2 cut(s) 60, 303
BshFI GGCC 7 cut(s) 59, 90, 107, 137, 302, 346, 648
BsiEI CGRYCG 2 cut(s) 60, 303
BsiHKCI CYCGRG 1 cut(s) 85
BsiSI CCGG 1 cut(s) 86
BslI CCNNNNNNNGG 2 cut(s) 103, 655
BsmAI GTCTC 2 cut(s) 26, 32
BsnI GGCC 7 cut(s) 59, 90, 107, 137, 302, 346, 648
Bso31I GGTCTC 2 cut(s) 26, 32
BsoBI CYCGRG 1 cut(s) 85
Bsp143I GATC 2 cut(s) 352, 547
BspANI GGCC 7 cut(s) 59, 90, 107, 137, 302, 346, 648
BspCNI CTCAG 1 cut(s) 406
BspFNI CGCG 2 cut(s) 53, 182
BspLI GGNNCC 3 cut(s) 282, 464, 514
BspMI ACCTGC 1 cut(s) 651
BspPI GGATC 1 cut(s) 555
BspQI GCTCTTC 1 cut(s) 216
BspTNI GGTCTC 2 cut(s) 26, 32
BsrBI CCGCTC 1 cut(s) 168
BsrDI GCAATG 1 cut(s) 337
BsrI ACTGG 1 cut(s) 516
BssECI CCNNGG 6 cut(s) 51, 60, 84, 85, 138, 649
BssMI GATC 2 cut(s) 352, 547
Bst2UI CCWGG 2 cut(s) 139, 446
Bst4CI ACNGT 1 cut(s) 16
Bst6I CTCTTC 3 cut(s) 156, 216, 281
BstC8I GCNNGC 3 cut(s) 135, 344, 641
BstDEI CTNAG 3 cut(s) 129, 414, 604
BstDSI CCRYGG 2 cut(s) 51, 649
BstFNI CGCG 2 cut(s) 53, 182
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 2 cut(s) 355, 550
BstMAI GTCTC 2 cut(s) 26, 32
BstMBI GATC 2 cut(s) 352, 547
BstMCI CGRYCG 2 cut(s) 60, 303
BstMWI GCNNNNNNNGC 6 cut(s) 17, 26, 177, 394, 599, 645
BstNI CCWGG 2 cut(s) 139, 446
BstSCI CCNGG 4 cut(s) 84, 85, 137, 444
BstUI CGCG 2 cut(s) 53, 182
BstV2I GAAGAC 1 cut(s) 197
BstZI CGGCCG 2 cut(s) 57, 300
Bsu36I CCTNAGG 1 cut(s) 604
BsuRI GGCC 7 cut(s) 59, 90, 107, 137, 302, 346, 648
BtgI CCRYGG 2 cut(s) 51, 649
BtsIMutI CAGTG 1 cut(s) 523
BveI ACCTGC 1 cut(s) 651
Cac8I GCNNGC 3 cut(s) 135, 344, 641
CfoI GCGC 1 cut(s) 182
Cfr13I GGNCC 5 cut(s) 70, 88, 106, 135, 281
Cfr42I CCGCGG 1 cut(s) 54
Cfr9I CCCGGG 1 cut(s) 85
CseI GACGC 1 cut(s) 128
CspCI CAANNNNNGTGG 2 cut(s) 604, 639
CviAII CATG 2 cut(s) 67, 574
DdeI CTNAG 3 cut(s) 129, 414, 604
DpnI GATC 2 cut(s) 354, 549
DpnII GATC 2 cut(s) 352, 547
EaeI YGGCCR 4 cut(s) 57, 300, 344, 646
EagI CGGCCG 2 cut(s) 57, 300
Eam1104I CTCTTC 3 cut(s) 156, 216, 281
EarI CTCTTC 3 cut(s) 156, 216, 281
EclXI CGGCCG 2 cut(s) 57, 300
Eco31I GGTCTC 2 cut(s) 26, 32
Eco47I GGWCC 2 cut(s) 70, 281
Eco52I CGGCCG 2 cut(s) 57, 300
Eco81I CCTNAGG 1 cut(s) 604
Eco88I CYCGRG 1 cut(s) 85
EcoRII CCWGG 2 cut(s) 137, 444
FaeI CATG 2 cut(s) 70, 577
FaiI YATR 7 cut(s) 68, 327, 372, 477, 552, 554, 575
FatI CATG 2 cut(s) 66, 573
FauI CCCGC 2 cut(s) 126, 256
Fnu4HI GCNGC 5 cut(s) 54, 57, 169, 172, 183
Fsp4HI GCNGC 5 cut(s) 54, 57, 169, 172, 183
GlaI GCGC 1 cut(s) 181
GluI GCNGC 5 cut(s) 54, 57, 169, 172, 183
HaeIII GGCC 7 cut(s) 59, 90, 107, 137, 302, 346, 648
HapII CCGG 1 cut(s) 86
HgaI GACGC 1 cut(s) 128
HhaI GCGC 1 cut(s) 182
Hin1II CATG 2 cut(s) 70, 577
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HinfI GANTC 1 cut(s) 441
HpaII CCGG 1 cut(s) 86
Hpy188I TCNGA 2 cut(s) 285, 415
Hpy188III TCNNGA 3 cut(s) 400, 535, 666
Hpy99I CGWCG 2 cut(s) 20, 122
HpyAV CCTTC 4 cut(s) 190, 242, 397, 648
HpyCH4III ACNGT 1 cut(s) 16
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 26, 177, 394, 599, 645
HpyF3I CTNAG 3 cut(s) 129, 414, 604
Hsp92II CATG 2 cut(s) 70, 577
HspAI GCGC 1 cut(s) 180
KspI CCGCGG 1 cut(s) 54
Kzo9I GATC 2 cut(s) 352, 547
LguI GCTCTTC 1 cut(s) 216
LmnI GCTCC 1 cut(s) 512
LweI GCATC 2 cut(s) 476, 580
MaeIII GTNAC 1 cut(s) 407
MalI GATC 2 cut(s) 354, 549
MbiI CCGCTC 1 cut(s) 168
MboI GATC 2 cut(s) 352, 547
MlsI TGGCCA 1 cut(s) 346
MluNI TGGCCA 1 cut(s) 346
MnlI CCTC 8 cut(s) 55, 83, 97, 196, 199, 253, 431, 626
Mox20I TGGCCA 1 cut(s) 346
MroXI GAANNNNTTC 1 cut(s) 290
MscI TGGCCA 1 cut(s) 346
MseI TTAA 2 cut(s) 357, 363
MslI CAYNNNNRTG 1 cut(s) 588
Msp20I TGGCCA 1 cut(s) 346
MspA1I CMGCKG 2 cut(s) 53, 218
MspI CCGG 1 cut(s) 86
MspR9I CCNGG 4 cut(s) 86, 87, 139, 446
MvaI CCWGG 2 cut(s) 139, 446
MvnI CGCG 2 cut(s) 53, 182
MwoI GCNNNNNNNGC 6 cut(s) 17, 26, 177, 394, 599, 645
NciI CCSGG 2 cut(s) 86, 87
NdeII GATC 2 cut(s) 352, 547
NlaIII CATG 2 cut(s) 70, 577
NlaIV GGNNCC 3 cut(s) 282, 464, 514
NmeAIII GCCGAG 2 cut(s) 55, 85
NmuCI GTSAC 1 cut(s) 407
PciSI GCTCTTC 1 cut(s) 216
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 300
PdmI GAANNNNTTC 1 cut(s) 290
PfeI GAWTC 1 cut(s) 441
PkrI GCNGC 5 cut(s) 55, 58, 170, 173, 184
PshBI ATTAAT 1 cut(s) 363
Psp6I CCWGG 2 cut(s) 137, 444
PspGI CCWGG 2 cut(s) 137, 444
PspN4I GGNNCC 3 cut(s) 282, 464, 514
PspPI GGNCC 5 cut(s) 70, 88, 106, 135, 281
RseI CAYNNNNRTG 1 cut(s) 588
SacII CCGCGG 1 cut(s) 54
SapI GCTCTTC 1 cut(s) 216
SaqAI TTAA 2 cut(s) 357, 363
SatI GCNGC 5 cut(s) 54, 57, 169, 172, 183
Sau3AI GATC 2 cut(s) 352, 547
Sau96I GGNCC 5 cut(s) 70, 88, 106, 135, 281
ScrFI CCNGG 4 cut(s) 86, 87, 139, 446
SetI ASST 6 cut(s) 75, 201, 278, 333, 641, 665
SfaNI GCATC 2 cut(s) 476, 580
Sfr303I CCGCGG 1 cut(s) 54
SgrBI CCGCGG 1 cut(s) 54
SinI GGWCC 2 cut(s) 70, 281
SmaI CCCGGG 1 cut(s) 87
SmiMI CAYNNNNRTG 1 cut(s) 588
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
SspI AATATT 1 cut(s) 361
StyD4I CCNGG 4 cut(s) 84, 85, 137, 444
TaaI ACNGT 1 cut(s) 16
TaqI TCGA 2 cut(s) 75, 315
TaqII GACCGA 1 cut(s) 50
TauI GCSGC 5 cut(s) 56, 59, 171, 174, 185
TfiI GAWTC 1 cut(s) 441
Tru1I TTAA 2 cut(s) 357, 363
Tru9I TTAA 2 cut(s) 357, 363
TscAI CASTG 1 cut(s) 523
TseFI GTSAC 1 cut(s) 407
Tsp45I GTSAC 1 cut(s) 407
TspDTI ATGAA 2 cut(s) 387, 590
TspMI CCCGGG 1 cut(s) 85
TspRI CASTG 1 cut(s) 523
VpaK11BI GGWCC 2 cut(s) 70, 281
VspI ATTAAT 1 cut(s) 363
XmaI CCCGGG 1 cut(s) 85
XmnI GAANNNNTTC 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.