RLG00000005192

Protein of unknown function (DUF3223)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0014579
Physical Location & Seq
Reverse (-)
65735328 .. 65737132
1805 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005192

Sequence Viewer

Length: 663 bp
ATGGCAGAGGCAACCGTCGCTGAAATCGCCGAGACCAACACCAACACCGCGGCGGCCGAGGACATGGACCTCGAAACCGCCCCGGCCCAAAACCCAGACGAGGCCCAGACCGGCGACGCAAACTCTAAGCGGGCCAGGGAAGAAGAAGAAGAGAGCGGTGGCGACGGCGCGGCGAAGAAGACGAAGGTGGAGGACGAGAAGTCAGCGGAAGAGCAAAGATTGGAGAAGTTAGGGGAAGGAGAGAGCGAGGCGGGTCGGGTCATCTTGGGTCCGAAGAGTTTCGGGTCGGCCGTTGAGATGTTCGACTATTTCTATAAGCTACTCCATTACTGGCCAACCGATCTTAATATTAATAAGTATGAATATCTGGTTTTGCTGGACTTGCTCAAGAAGGGTCACTCTGAGCCTGATAAAAAGATTGGTGGAGGAATCCAGGCTTTCCAAGTTCGGTTCCACCCATTGTATAGAAGTAGATGCTACTTCCTTGTTAGAGAAGATGGAGCCAGTGATGATTTTAGTTTCAGGAAGTGTGTGGATCATATACTTCCCTTGCCAGAGAACATGAAAGCACATGCTGATGCCAACAAAGCCTTAGGCGGTGGCAAGGGTGGTGGGGGGAGAGGAAGCTGGCGAGGCCGTGGAAGGGGCAGGTCAAGAAACTGA

Protein Analysis

221

Amino Acids

24.23

Weight (kDa)

5.4

Isoelectric Point (pI)

40.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 106 - 178 2.3e-25 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0014579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62440
fragaria_vesca FvH4_5g14920
malus_domestica MD06G1193500.v1.1 MD14G1200600.v1.1
prunus_persica Prupe.5G193800_v2.0.a1
pyrus_communis pycom06g17160 pycom14g16630
rosa_chinensis RchiOBHm_Chr7g0181461
rosa_laevigata RLG00000005192
rosa_multiflora Rmu_sc0004621.1_g000022
rosa_roxburghii Rroxscaffold_3G00271980
rosa_rugosa Rorug06G0441500
rosa_samantha Rh7AG043300 Rh7BG042700 Rh7CG044500 Rh7DG043100
rosa_wichuraiana Rw7G003600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 639
AccBSI CCGCTC 1 cut(s) 156
AccII CGCG 2 cut(s) 50, 170
AclWI GGATC 1 cut(s) 543
AcoI YGGCCR 3 cut(s) 54, 288, 332
AfiI CCNNNNNNNGG 2 cut(s) 100, 643
AhdI GACNNNNNGTC 1 cut(s) 199
AjnI CCWGG 2 cut(s) 134, 432
AluBI AGCT 2 cut(s) 319, 627
AluI AGCT 2 cut(s) 319, 627
Alw26I GTCTC 1 cut(s) 26
AlwI GGATC 1 cut(s) 543
AoxI GGCC 7 cut(s) 54, 84, 102, 132, 288, 332, 634
AseI ATTAAT 1 cut(s) 351
Asp700I GAANNNNTTC 1 cut(s) 278
AspLEI GCGC 1 cut(s) 170
AspS9I GGNCC 5 cut(s) 67, 85, 103, 132, 269
AsuC2I CCSGG 1 cut(s) 83
AvaII GGWCC 2 cut(s) 67, 269
AxyI CCTNAGG 1 cut(s) 592
BalI TGGCCA 1 cut(s) 334
BbsI GAAGAC 1 cut(s) 185
BccI CCATC 1 cut(s) 491
BceAI ACGGC 3 cut(s) 181, 275, 621
BciT130I CCWGG 2 cut(s) 136, 434
BcnI CCSGG 1 cut(s) 83
BcoDI GTCTC 1 cut(s) 26
BfuAI ACCTGC 1 cut(s) 639
BisI GCNGC 3 cut(s) 51, 54, 171
BlsI GCNGC 3 cut(s) 52, 55, 172
Bme1390I CCNGG 3 cut(s) 83, 136, 434
Bme18I GGWCC 2 cut(s) 67, 269
BmeRI GACNNNNNGTC 1 cut(s) 199
BmgT120I GGNCC 5 cut(s) 67, 85, 103, 132, 269
BmiI GGNNCC 3 cut(s) 270, 452, 502
BmrFI CCNGG 3 cut(s) 83, 136, 434
BmsI GCATC 2 cut(s) 464, 568
BpiI GAAGAC 1 cut(s) 185
BpuEI CTTGAG 1 cut(s) 371
BpuMI CCSGG 1 cut(s) 83
BsaI GGTCTC 1 cut(s) 26
BsaJI CCNNGG 5 cut(s) 48, 57, 81, 135, 637
Bsc4I CCNNNNNNNGG 2 cut(s) 100, 643
Bse118I RCCGGY 1 cut(s) 110
Bse1I ACTGG 2 cut(s) 335, 504
Bse21I CCTNAGG 1 cut(s) 592
BseBI CCWGG 2 cut(s) 136, 434
BseDI CCNNGG 5 cut(s) 48, 57, 81, 135, 637
BseLI CCNNNNNNNGG 2 cut(s) 100, 643
BseMII CTCAG 1 cut(s) 393
BseNI ACTGG 2 cut(s) 335, 504
BseX3I CGGCCG 2 cut(s) 54, 288
Bsh1236I CGCG 2 cut(s) 50, 170
Bsh1285I CGRYCG 2 cut(s) 57, 291
BshFI GGCC 7 cut(s) 56, 86, 104, 134, 290, 334, 636
BsiEI CGRYCG 2 cut(s) 57, 291
BsiSI CCGG 2 cut(s) 83, 111
BslI CCNNNNNNNGG 2 cut(s) 100, 643
BsmAI GTCTC 1 cut(s) 26
BsnI GGCC 7 cut(s) 56, 86, 104, 134, 290, 334, 636
Bso31I GGTCTC 1 cut(s) 26
Bsp143I GATC 2 cut(s) 340, 535
BspANI GGCC 7 cut(s) 56, 86, 104, 134, 290, 334, 636
BspCNI CTCAG 1 cut(s) 394
BspFNI CGCG 2 cut(s) 50, 170
BspLI GGNNCC 3 cut(s) 270, 452, 502
BspMI ACCTGC 1 cut(s) 639
BspPI GGATC 1 cut(s) 543
BspQI GCTCTTC 1 cut(s) 204
BspTNI GGTCTC 1 cut(s) 26
BsrBI CCGCTC 1 cut(s) 156
BsrFI RCCGGY 1 cut(s) 110
BsrI ACTGG 2 cut(s) 335, 504
BssAI RCCGGY 1 cut(s) 110
BssECI CCNNGG 5 cut(s) 48, 57, 81, 135, 637
BssMI GATC 2 cut(s) 340, 535
Bst2UI CCWGG 2 cut(s) 136, 434
Bst4CI ACNGT 1 cut(s) 16
Bst6I CTCTTC 3 cut(s) 144, 204, 269
BstC8I GCNNGC 2 cut(s) 132, 629
BstDEI CTNAG 3 cut(s) 126, 402, 592
BstDSI CCRYGG 2 cut(s) 48, 637
BstFNI CGCG 2 cut(s) 50, 170
BstHHI GCGC 1 cut(s) 170
BstKTI GATC 2 cut(s) 343, 538
BstMAI GTCTC 1 cut(s) 26
BstMBI GATC 2 cut(s) 340, 535
BstMCI CGRYCG 2 cut(s) 57, 291
BstMWI GCNNNNNNNGC 5 cut(s) 17, 26, 382, 587, 633
BstNI CCWGG 2 cut(s) 136, 434
BstNSI RCATGY 1 cut(s) 575
BstSCI CCNGG 3 cut(s) 81, 134, 432
BstUI CGCG 2 cut(s) 50, 170
BstV2I GAAGAC 1 cut(s) 185
BstZI CGGCCG 2 cut(s) 54, 288
Bsu36I CCTNAGG 1 cut(s) 592
BsuRI GGCC 7 cut(s) 56, 86, 104, 134, 290, 334, 636
BtgI CCRYGG 2 cut(s) 48, 637
BtsIMutI CAGTG 1 cut(s) 511
BveI ACCTGC 1 cut(s) 639
Cac8I GCNNGC 2 cut(s) 132, 629
CfoI GCGC 1 cut(s) 170
Cfr10I RCCGGY 1 cut(s) 110
Cfr13I GGNCC 5 cut(s) 67, 85, 103, 132, 269
Cfr42I CCGCGG 1 cut(s) 51
CseI GACGC 1 cut(s) 125
CspCI CAANNNNNGTGG 2 cut(s) 592, 627
CviAII CATG 3 cut(s) 64, 562, 572
DdeI CTNAG 3 cut(s) 126, 402, 592
DpnI GATC 2 cut(s) 342, 537
DpnII GATC 2 cut(s) 340, 535
DriI GACNNNNNGTC 1 cut(s) 199
EaeI YGGCCR 3 cut(s) 54, 288, 332
EagI CGGCCG 2 cut(s) 54, 288
Eam1104I CTCTTC 3 cut(s) 144, 204, 269
Eam1105I GACNNNNNGTC 1 cut(s) 199
EarI CTCTTC 3 cut(s) 144, 204, 269
EclXI CGGCCG 2 cut(s) 54, 288
Eco31I GGTCTC 1 cut(s) 26
Eco47I GGWCC 2 cut(s) 67, 269
Eco52I CGGCCG 2 cut(s) 54, 288
Eco81I CCTNAGG 1 cut(s) 592
EcoRII CCWGG 2 cut(s) 134, 432
FaeI CATG 3 cut(s) 67, 565, 575
FaiI YATR 8 cut(s) 65, 315, 360, 465, 540, 542, 563, 573
FatI CATG 3 cut(s) 63, 561, 571
FauI CCCGC 2 cut(s) 123, 244
Fnu4HI GCNGC 3 cut(s) 51, 54, 171
Fsp4HI GCNGC 3 cut(s) 51, 54, 171
GlaI GCGC 1 cut(s) 169
GluI GCNGC 3 cut(s) 51, 54, 171
HaeIII GGCC 7 cut(s) 56, 86, 104, 134, 290, 334, 636
HapII CCGG 2 cut(s) 83, 111
HgaI GACGC 1 cut(s) 125
HhaI GCGC 1 cut(s) 170
Hin1II CATG 3 cut(s) 67, 565, 575
Hin6I GCGC 1 cut(s) 168
HinP1I GCGC 1 cut(s) 168
HinfI GANTC 1 cut(s) 429
HpaII CCGG 2 cut(s) 83, 111
Hpy188I TCNGA 2 cut(s) 273, 403
Hpy188III TCNNGA 3 cut(s) 388, 523, 654
Hpy99I CGWCG 3 cut(s) 20, 119, 167
HpyAV CCTTC 4 cut(s) 178, 230, 385, 636
HpyCH4III ACNGT 1 cut(s) 16
HpyF10VI GCNNNNNNNGC 5 cut(s) 17, 26, 382, 587, 633
HpyF3I CTNAG 3 cut(s) 126, 402, 592
Hsp92II CATG 3 cut(s) 67, 565, 575
HspAI GCGC 1 cut(s) 168
KspI CCGCGG 1 cut(s) 51
Kzo9I GATC 2 cut(s) 340, 535
LguI GCTCTTC 1 cut(s) 204
LmnI GCTCC 1 cut(s) 500
LweI GCATC 2 cut(s) 464, 568
MaeIII GTNAC 1 cut(s) 395
MalI GATC 2 cut(s) 342, 537
MbiI CCGCTC 1 cut(s) 156
MboI GATC 2 cut(s) 340, 535
MboII GAAGA 9 cut(s) 152, 155, 158, 161, 187, 190, 221, 286, 506
MlsI TGGCCA 1 cut(s) 334
MluNI TGGCCA 1 cut(s) 334
MnlI CCTC 8 cut(s) 52, 80, 94, 184, 241, 419, 614, 626
Mox20I TGGCCA 1 cut(s) 334
MroXI GAANNNNTTC 1 cut(s) 278
MscI TGGCCA 1 cut(s) 334
MseI TTAA 2 cut(s) 345, 351
MslI CAYNNNNRTG 1 cut(s) 576
Msp20I TGGCCA 1 cut(s) 334
MspA1I CMGCKG 2 cut(s) 50, 206
MspI CCGG 2 cut(s) 83, 111
MspR9I CCNGG 3 cut(s) 83, 136, 434
MvaI CCWGG 2 cut(s) 136, 434
MvnI CGCG 2 cut(s) 50, 170
MwoI GCNNNNNNNGC 5 cut(s) 17, 26, 382, 587, 633
NciI CCSGG 1 cut(s) 83
NdeII GATC 2 cut(s) 340, 535
NlaIII CATG 3 cut(s) 67, 565, 575
NlaIV GGNNCC 3 cut(s) 270, 452, 502
NmeAIII GCCGAG 2 cut(s) 55, 82
NmuCI GTSAC 1 cut(s) 395
NspI RCATGY 1 cut(s) 575
PciSI GCTCTTC 1 cut(s) 204
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PdmI GAANNNNTTC 1 cut(s) 278
PfeI GAWTC 1 cut(s) 429
PkrI GCNGC 3 cut(s) 52, 55, 172
PshBI ATTAAT 1 cut(s) 351
Psp6I CCWGG 2 cut(s) 134, 432
PspGI CCWGG 2 cut(s) 134, 432
PspN4I GGNNCC 3 cut(s) 270, 452, 502
PspPI GGNCC 5 cut(s) 67, 85, 103, 132, 269
RseI CAYNNNNRTG 1 cut(s) 576
SacII CCGCGG 1 cut(s) 51
SapI GCTCTTC 1 cut(s) 204
SaqAI TTAA 2 cut(s) 345, 351
SatI GCNGC 3 cut(s) 51, 54, 171
Sau3AI GATC 2 cut(s) 340, 535
Sau96I GGNCC 5 cut(s) 67, 85, 103, 132, 269
ScrFI CCNGG 3 cut(s) 83, 136, 434
SetI ASST 5 cut(s) 72, 189, 321, 629, 653
SfaNI GCATC 2 cut(s) 464, 568
Sfr303I CCGCGG 1 cut(s) 51
SgrBI CCGCGG 1 cut(s) 51
SinI GGWCC 2 cut(s) 67, 269
SmiMI CAYNNNNRTG 1 cut(s) 576
SmlI CTYRAG 1 cut(s) 386
SmoI CTYRAG 1 cut(s) 386
SspI AATATT 1 cut(s) 349
StyD4I CCNGG 3 cut(s) 81, 134, 432
TaaI ACNGT 1 cut(s) 16
TaqI TCGA 2 cut(s) 72, 303
TauI GCSGC 3 cut(s) 53, 56, 173
TfiI GAWTC 1 cut(s) 429
Tru1I TTAA 2 cut(s) 345, 351
Tru9I TTAA 2 cut(s) 345, 351
TscAI CASTG 1 cut(s) 511
TseFI GTSAC 1 cut(s) 395
Tsp45I GTSAC 1 cut(s) 395
TspDTI ATGAA 2 cut(s) 375, 578
TspRI CASTG 1 cut(s) 511
VpaK11BI GGWCC 2 cut(s) 67, 269
VspI ATTAAT 1 cut(s) 351
XceI RCATGY 1 cut(s) 575
XmnI GAANNNNTTC 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.