Rw7G003600

Protein of unknown function (DUF3223)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0014579
Physical Location & Seq
Forward (+)
2954290 .. 2956403
2114 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G003600.1

Sequence Viewer

Length: 672 bp
ATGGCAGAGGCAACCGTCGCTGAAATCGCCGAGACCGAGACCGAGACCAACATCAACACCGCGGCGGCGGAGGACATGGACCTCGAAACCGCCCCGGCCCAAAACCCAGACGAGGCCCAGAACGGCGACGCAAACTCTAAGCGGGCCAGGGAAGAAGAAGAGAGCGGCGGCGACGGCGCGGCGAAGAAGACGAAGGTGGAGGAGGAGAAGTCAGCGGAAGAGCAACGATTGGAGAAGTTAGGGGAAGGAGAGAGCGAGGCGGGTCGGGTCATCTTGGGTCCGAAGAGTTTCGGGTCGGCCGTTGAGATGTTCGACTATTTCTATAAGCTACTCCATTACTGGCCAACCGATCTTAATATTAATAAGTATGAATATCTGGTTTTGCTGGACTTGCTCAAGAAGGGTCACTCTGAGCCTGATAAAAAGATTGGTGGAGGAATCCAGGCTTTCCAAGTTCGGTTCCACCCATTGTATAGAAGTAGATGCTACTTCCTTGTTAGAGAAGATGGAGCCAGTGATGATTTTAGTTTCAGGAAGTGTGTGGATCATATACTTCCCTTGCCAGAGAACATGAAAGCACACGCTGATGCCAACAAAGCCTTAGGCGGTGGCAAGGGTGGTGGGGGGAGAGGAAGCTGGCGTGGCCGTGGAAGGGGCAGGTCAAGAAACTGA

Protein Analysis

223

Amino Acids

24.6

Weight (kDa)

5.31

Isoelectric Point (pI)

45.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 109 - 181 2.4e-25 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62440
fragaria_vesca FvH4_5g14920
malus_domestica MD06G1193500.v1.1 MD14G1200600.v1.1
prunus_persica Prupe.5G193800_v2.0.a1
pyrus_communis pycom06g17160 pycom14g16630
rosa_chinensis RchiOBHm_Chr7g0181461
rosa_laevigata RLG00000005192
rosa_multiflora Rmu_sc0004621.1_g000022
rosa_roxburghii Rroxscaffold_3G00271980
rosa_rugosa Rorug06G0441500
rosa_samantha Rh7AG043300 Rh7BG042700 Rh7CG044500 Rh7DG043100
rosa_wichuraiana Rw7G003600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 648
AccBSI CCGCTC 1 cut(s) 165
AccII CGCG 2 cut(s) 62, 179
AclWI GGATC 1 cut(s) 552
AcoI YGGCCR 3 cut(s) 297, 341, 643
AfiI CCNNNNNNNGG 2 cut(s) 112, 652
AjnI CCWGG 2 cut(s) 146, 441
AluBI AGCT 2 cut(s) 328, 636
AluI AGCT 2 cut(s) 328, 636
Alw26I GTCTC 3 cut(s) 26, 32, 38
AlwI GGATC 1 cut(s) 552
AoxI GGCC 6 cut(s) 96, 114, 144, 297, 341, 643
AseI ATTAAT 1 cut(s) 360
Asp700I GAANNNNTTC 1 cut(s) 287
AspLEI GCGC 1 cut(s) 179
AspS9I GGNCC 5 cut(s) 79, 97, 115, 144, 278
AsuC2I CCSGG 1 cut(s) 95
AvaII GGWCC 2 cut(s) 79, 278
AxyI CCTNAGG 1 cut(s) 601
BalI TGGCCA 1 cut(s) 343
BbsI GAAGAC 1 cut(s) 194
BccI CCATC 1 cut(s) 500
BceAI ACGGC 4 cut(s) 139, 190, 284, 630
BciT130I CCWGG 2 cut(s) 148, 443
BcnI CCSGG 1 cut(s) 95
BcoDI GTCTC 3 cut(s) 26, 32, 38
BfuAI ACCTGC 1 cut(s) 648
BisI GCNGC 5 cut(s) 63, 66, 166, 169, 180
BlsI GCNGC 5 cut(s) 64, 67, 167, 170, 181
Bme1390I CCNGG 3 cut(s) 95, 148, 443
Bme18I GGWCC 2 cut(s) 79, 278
BmgT120I GGNCC 5 cut(s) 79, 97, 115, 144, 278
BmiI GGNNCC 3 cut(s) 279, 461, 511
BmrFI CCNGG 3 cut(s) 95, 148, 443
BmsI GCATC 2 cut(s) 473, 577
BpiI GAAGAC 1 cut(s) 194
BpuEI CTTGAG 1 cut(s) 380
BpuMI CCSGG 1 cut(s) 95
BsaI GGTCTC 3 cut(s) 26, 32, 38
BsaJI CCNNGG 4 cut(s) 60, 93, 147, 646
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 2 cut(s) 112, 652
Bse1I ACTGG 2 cut(s) 344, 513
Bse21I CCTNAGG 1 cut(s) 601
BseBI CCWGG 2 cut(s) 148, 443
BseDI CCNNGG 4 cut(s) 60, 93, 147, 646
BseLI CCNNNNNNNGG 2 cut(s) 112, 652
BseMII CTCAG 1 cut(s) 402
BseNI ACTGG 2 cut(s) 344, 513
BseRI GAGGAG 2 cut(s) 215, 218
BseX3I CGGCCG 1 cut(s) 297
Bsh1236I CGCG 2 cut(s) 62, 179
Bsh1285I CGRYCG 1 cut(s) 300
BshFI GGCC 6 cut(s) 98, 116, 146, 299, 343, 645
BsiEI CGRYCG 1 cut(s) 300
BsiSI CCGG 1 cut(s) 95
BslI CCNNNNNNNGG 2 cut(s) 112, 652
BsmAI GTCTC 3 cut(s) 26, 32, 38
BsnI GGCC 6 cut(s) 98, 116, 146, 299, 343, 645
Bso31I GGTCTC 3 cut(s) 26, 32, 38
Bsp143I GATC 2 cut(s) 349, 544
BspANI GGCC 6 cut(s) 98, 116, 146, 299, 343, 645
BspCNI CTCAG 1 cut(s) 403
BspFNI CGCG 2 cut(s) 62, 179
BspLI GGNNCC 3 cut(s) 279, 461, 511
BspMI ACCTGC 1 cut(s) 648
BspPI GGATC 1 cut(s) 552
BspQI GCTCTTC 1 cut(s) 213
BspTNI GGTCTC 3 cut(s) 26, 32, 38
BsrBI CCGCTC 1 cut(s) 165
BsrI ACTGG 2 cut(s) 344, 513
BssECI CCNNGG 4 cut(s) 60, 93, 147, 646
BssMI GATC 2 cut(s) 349, 544
Bst2UI CCWGG 2 cut(s) 148, 443
Bst4CI ACNGT 1 cut(s) 16
Bst6I CTCTTC 3 cut(s) 153, 213, 278
BstC8I GCNNGC 2 cut(s) 144, 638
BstDEI CTNAG 3 cut(s) 138, 411, 601
BstDSI CCRYGG 2 cut(s) 60, 646
BstFNI CGCG 2 cut(s) 62, 179
BstHHI GCGC 1 cut(s) 179
BstKTI GATC 2 cut(s) 352, 547
BstMAI GTCTC 3 cut(s) 26, 32, 38
BstMBI GATC 2 cut(s) 349, 544
BstMCI CGRYCG 1 cut(s) 300
BstMWI GCNNNNNNNGC 6 cut(s) 17, 26, 174, 391, 596, 642
BstNI CCWGG 2 cut(s) 148, 443
BstSCI CCNGG 3 cut(s) 93, 146, 441
BstUI CGCG 2 cut(s) 62, 179
BstV2I GAAGAC 1 cut(s) 194
BstZI CGGCCG 1 cut(s) 297
Bsu36I CCTNAGG 1 cut(s) 601
BsuRI GGCC 6 cut(s) 98, 116, 146, 299, 343, 645
BtgI CCRYGG 2 cut(s) 60, 646
BtsIMutI CAGTG 1 cut(s) 520
BveI ACCTGC 1 cut(s) 648
Cac8I GCNNGC 2 cut(s) 144, 638
CfoI GCGC 1 cut(s) 179
Cfr13I GGNCC 5 cut(s) 79, 97, 115, 144, 278
Cfr42I CCGCGG 1 cut(s) 63
CseI GACGC 1 cut(s) 137
CspCI CAANNNNNGTGG 2 cut(s) 601, 636
CviAII CATG 2 cut(s) 76, 571
DdeI CTNAG 3 cut(s) 138, 411, 601
DpnI GATC 2 cut(s) 351, 546
DpnII GATC 2 cut(s) 349, 544
EaeI YGGCCR 3 cut(s) 297, 341, 643
EagI CGGCCG 1 cut(s) 297
Eam1104I CTCTTC 3 cut(s) 153, 213, 278
EarI CTCTTC 3 cut(s) 153, 213, 278
EciI GGCGGA 1 cut(s) 83
EclXI CGGCCG 1 cut(s) 297
Eco31I GGTCTC 3 cut(s) 26, 32, 38
Eco47I GGWCC 2 cut(s) 79, 278
Eco52I CGGCCG 1 cut(s) 297
Eco81I CCTNAGG 1 cut(s) 601
EcoRII CCWGG 2 cut(s) 146, 441
FaeI CATG 2 cut(s) 79, 574
FaiI YATR 7 cut(s) 77, 324, 369, 474, 549, 551, 572
FatI CATG 2 cut(s) 75, 570
FauI CCCGC 2 cut(s) 135, 253
Fnu4HI GCNGC 5 cut(s) 63, 66, 166, 169, 180
Fsp4HI GCNGC 5 cut(s) 63, 66, 166, 169, 180
GlaI GCGC 1 cut(s) 178
GluI GCNGC 5 cut(s) 63, 66, 166, 169, 180
HaeIII GGCC 6 cut(s) 98, 116, 146, 299, 343, 645
HapII CCGG 1 cut(s) 95
HgaI GACGC 1 cut(s) 137
HhaI GCGC 1 cut(s) 179
Hin1II CATG 2 cut(s) 79, 574
Hin6I GCGC 1 cut(s) 177
HinP1I GCGC 1 cut(s) 177
HinfI GANTC 1 cut(s) 438
HpaII CCGG 1 cut(s) 95
Hpy188I TCNGA 2 cut(s) 282, 412
Hpy188III TCNNGA 3 cut(s) 397, 532, 663
Hpy99I CGWCG 3 cut(s) 20, 131, 176
HpyAV CCTTC 4 cut(s) 187, 239, 394, 645
HpyCH4III ACNGT 1 cut(s) 16
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 26, 174, 391, 596, 642
HpyF3I CTNAG 3 cut(s) 138, 411, 601
Hsp92II CATG 2 cut(s) 79, 574
HspAI GCGC 1 cut(s) 177
KspI CCGCGG 1 cut(s) 63
Kzo9I GATC 2 cut(s) 349, 544
LguI GCTCTTC 1 cut(s) 213
LmnI GCTCC 1 cut(s) 509
LweI GCATC 2 cut(s) 473, 577
MaeIII GTNAC 1 cut(s) 404
MalI GATC 2 cut(s) 351, 546
MbiI CCGCTC 1 cut(s) 165
MboI GATC 2 cut(s) 349, 544
MboII GAAGA 8 cut(s) 164, 167, 170, 196, 199, 230, 295, 515
MlsI TGGCCA 1 cut(s) 343
MluNI TGGCCA 1 cut(s) 343
MnlI CCTC 8 cut(s) 64, 92, 106, 193, 196, 250, 428, 623
Mox20I TGGCCA 1 cut(s) 343
MroXI GAANNNNTTC 1 cut(s) 287
MscI TGGCCA 1 cut(s) 343
MseI TTAA 2 cut(s) 354, 360
MslI CAYNNNNRTG 1 cut(s) 585
Msp20I TGGCCA 1 cut(s) 343
MspA1I CMGCKG 2 cut(s) 62, 215
MspI CCGG 1 cut(s) 95
MspR9I CCNGG 3 cut(s) 95, 148, 443
MvaI CCWGG 2 cut(s) 148, 443
MvnI CGCG 2 cut(s) 62, 179
MwoI GCNNNNNNNGC 6 cut(s) 17, 26, 174, 391, 596, 642
NciI CCSGG 1 cut(s) 95
NdeII GATC 2 cut(s) 349, 544
NlaIII CATG 2 cut(s) 79, 574
NlaIV GGNNCC 3 cut(s) 279, 461, 511
NmeAIII GCCGAG 1 cut(s) 55
NmuCI GTSAC 1 cut(s) 404
PciSI GCTCTTC 1 cut(s) 213
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 297
PdmI GAANNNNTTC 1 cut(s) 287
PfeI GAWTC 1 cut(s) 438
PkrI GCNGC 5 cut(s) 64, 67, 167, 170, 181
PshBI ATTAAT 1 cut(s) 360
Psp6I CCWGG 2 cut(s) 146, 441
PspGI CCWGG 2 cut(s) 146, 441
PspN4I GGNNCC 3 cut(s) 279, 461, 511
PspPI GGNCC 5 cut(s) 79, 97, 115, 144, 278
RseI CAYNNNNRTG 1 cut(s) 585
SacII CCGCGG 1 cut(s) 63
SapI GCTCTTC 1 cut(s) 213
SaqAI TTAA 2 cut(s) 354, 360
SatI GCNGC 5 cut(s) 63, 66, 166, 169, 180
Sau3AI GATC 2 cut(s) 349, 544
Sau96I GGNCC 5 cut(s) 79, 97, 115, 144, 278
ScrFI CCNGG 3 cut(s) 95, 148, 443
SetI ASST 5 cut(s) 84, 198, 330, 638, 662
SfaNI GCATC 2 cut(s) 473, 577
Sfr303I CCGCGG 1 cut(s) 63
SgrBI CCGCGG 1 cut(s) 63
SinI GGWCC 2 cut(s) 79, 278
SmiMI CAYNNNNRTG 1 cut(s) 585
SmlI CTYRAG 1 cut(s) 395
SmoI CTYRAG 1 cut(s) 395
SspI AATATT 1 cut(s) 358
StyD4I CCNGG 3 cut(s) 93, 146, 441
TaaI ACNGT 1 cut(s) 16
TaqI TCGA 2 cut(s) 84, 312
TaqII GACCGA 2 cut(s) 50, 56
TauI GCSGC 5 cut(s) 65, 68, 168, 171, 182
TfiI GAWTC 1 cut(s) 438
Tru1I TTAA 2 cut(s) 354, 360
Tru9I TTAA 2 cut(s) 354, 360
TscAI CASTG 1 cut(s) 520
TseFI GTSAC 1 cut(s) 404
Tsp45I GTSAC 1 cut(s) 404
TspDTI ATGAA 2 cut(s) 384, 587
TspRI CASTG 1 cut(s) 520
VpaK11BI GGWCC 2 cut(s) 79, 278
VspI ATTAAT 1 cut(s) 360
XmnI GAANNNNTTC 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.