AT5G62720

integral membrane HPP family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007927
Physical Location & Seq
Forward (+)
25191402 .. 25195071
3670 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G62720.1

Sequence Viewer

Length: 732 bp
ATGGCTTCTGTTCCGGTGAGACCTCTACCTCTTCTTCGCCGGAACATCACCTCGACGACGGCTTCGAAATCATCTCCAATGCTCGCCAACGTTTCGAGCCGGCATTCTCTCGGGATCTCTACGTATGATGAATTTTTGAAGCAAATAAAAACTCCGGCAACTGTGAACCACCGGCGGCGAGTAAGTACGGTGGTGGCGTCGGCGGGAAACTTGACGGCGCCGTCGTGGGATTCGTGGAAGCCTGATAAGACGGCGGCGGCTACGGCTCTTTTGCTGAGTGACGTCATATGGCCTGCTGCTGGAGCGTTTGCGGCAATGGCATTATTGGGAAGAATGGATCAAATGCTATCTCCGAAAGGCATTTCAATGTCAGTTGCACCACTTGGCGCAGTCTCCGCCATTCTTTTCATCACCCCTTCTGCTCCTGCTGCTCGGAAATACAATATATTTTTGGCTCAAATAGGTTGTGCTGCGATTGGGGTGGTAGCTTTCTCCGTCTTCGGCCCAGGCTGGCTCGCCCGGAGTGTCGCCCTCGCCGCTTCCATCGCTTTTATGGTCATTACTCGTGCCAATCACCCTCCTGCGGCGAGCTTACCACTAATGTTCATAGATGGAGCAAAGTTCCATCACTTGAATTTCTGGTACGCATTGTTCCCAGGTGCAGCTGCTTGTGTCATCCTCTGCCTTCTCCAATCGATCGTATGTTACTTGAAGGAAAACATGAAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HPP

Protein Analysis

243

Amino Acids

25.83

Weight (kDa)

10.14

Isoelectric Point (pI)

51.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TM_HPP PF04982 92 - 238 2.7e-31 HPP, transmembrane region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 220
AatII GACGTC 1 cut(s) 285
AccB1I GGYRCC 1 cut(s) 217
AciI CCGC 8 cut(s) 175, 203, 254, 257, 311, 396, 537, 584
AclI AACGTT 1 cut(s) 90
AclWI GGATC 2 cut(s) 122, 345
AcsI RAATTY 3 cut(s) 131, 634, 725
AcyI GRCGYC 3 cut(s) 197, 218, 282
AfaI GTAC 2 cut(s) 187, 644
AfiI CCNNNNNNNGG 2 cut(s) 299, 583
AgsI TTSAA 4 cut(s) 139, 366, 634, 712
AjnI CCWGG 2 cut(s) 505, 655
AluBI AGCT 3 cut(s) 488, 591, 665
AluI AGCT 3 cut(s) 488, 591, 665
Alw26I GTCTC 2 cut(s) 13, 397
AlwI GGATC 2 cut(s) 122, 345
Ama87I CYCGRG 1 cut(s) 110
AoxI GGCC 2 cut(s) 290, 502
ApeKI GCWGC 5 cut(s) 296, 428, 470, 662, 665
ApoI RAATTY 3 cut(s) 131, 634, 725
ArsI GACNNNNNNTTYG 2 cut(s) 46, 78
AspLEI GCGC 2 cut(s) 220, 389
AspS9I GGNCC 1 cut(s) 503
AsuC2I CCSGG 1 cut(s) 520
AsuHPI GGTGA 4 cut(s) 28, 40, 403, 566
AsuII TTCGAA 1 cut(s) 65
AvaI CYCGRG 1 cut(s) 110
BanI GGYRCC 1 cut(s) 217
BauI CACGAG 1 cut(s) 564
BbsI GAAGAC 1 cut(s) 490
BbvI GCAGC 5 cut(s) 283, 415, 457, 652, 674
BccI CCATC 3 cut(s) 551, 605, 633
BceAI ACGGC 5 cut(s) 75, 205, 231, 267, 279
BciT130I CCWGG 2 cut(s) 507, 657
BcnI CCSGG 1 cut(s) 520
BcoDI GTCTC 2 cut(s) 13, 397
BfoI RGCGCY 1 cut(s) 221
Bme1390I CCNGG 3 cut(s) 507, 520, 657
BmeT110I CYCGRG 1 cut(s) 110
BmgT120I GGNCC 1 cut(s) 503
BmiI GGNNCC 1 cut(s) 219
BmrFI CCNGG 3 cut(s) 507, 520, 657
BpiI GAAGAC 1 cut(s) 490
BpmI CTGGAG 1 cut(s) 321
Bpu14I TTCGAA 1 cut(s) 65
BpuMI CCSGG 1 cut(s) 520
Bsa29I ATCGAT 1 cut(s) 695
BsaAI YACGTR 1 cut(s) 123
BsaHI GRCGYC 3 cut(s) 197, 218, 282
BsaI GGTCTC 1 cut(s) 13
BsaJI CCNNGG 2 cut(s) 505, 655
BsaWI WCCGGW 1 cut(s) 13
Bsc4I CCNNNNNNNGG 2 cut(s) 299, 583
Bse118I RCCGGY 2 cut(s) 99, 171
Bse3DI GCAATG 1 cut(s) 321
BseBI CCWGG 2 cut(s) 507, 657
BseCI ATCGAT 1 cut(s) 695
BseDI CCNNGG 2 cut(s) 505, 655
BseGI GGATG 1 cut(s) 675
BseLI CCNNNNNNNGG 2 cut(s) 299, 583
BseMI GCAATG 1 cut(s) 321
BseMII CTCAG 1 cut(s) 266
BseXI GCAGC 5 cut(s) 283, 415, 457, 652, 674
BsgI GTGCAG 1 cut(s) 681
Bsh1285I CGRYCG 1 cut(s) 699
BshFI GGCC 2 cut(s) 292, 504
BshNI GGYRCC 1 cut(s) 217
BshVI ATCGAT 1 cut(s) 695
BsiEI CGRYCG 1 cut(s) 699
BsiHKCI CYCGRG 1 cut(s) 110
BsiSI CCGG 6 cut(s) 14, 40, 100, 155, 172, 520
BslI CCNNNNNNNGG 2 cut(s) 299, 583
BsmAI GTCTC 2 cut(s) 13, 397
BsmI GAATGC 1 cut(s) 103
BsnI GGCC 2 cut(s) 292, 504
Bso31I GGTCTC 1 cut(s) 13
BsoBI CYCGRG 1 cut(s) 110
Bsp119I TTCGAA 1 cut(s) 65
Bsp143I GATC 3 cut(s) 114, 337, 696
BspACI CCGC 8 cut(s) 175, 203, 254, 257, 311, 396, 537, 584
BspANI GGCC 2 cut(s) 292, 504
BspCNI CTCAG 1 cut(s) 267
BspDI ATCGAT 1 cut(s) 695
BspLI GGNNCC 1 cut(s) 219
BspPI GGATC 2 cut(s) 122, 345
BspT104I TTCGAA 1 cut(s) 65
BspT107I GGYRCC 1 cut(s) 217
BspTNI GGTCTC 1 cut(s) 13
BsrDI GCAATG 1 cut(s) 321
BsrFI RCCGGY 2 cut(s) 99, 171
BssAI RCCGGY 2 cut(s) 99, 171
BssECI CCNNGG 2 cut(s) 505, 655
BssMI GATC 3 cut(s) 114, 337, 696
BssNI GRCGYC 3 cut(s) 197, 218, 282
BssSI CACGAG 1 cut(s) 564
Bst2BI CACGAG 1 cut(s) 564
Bst2UI CCWGG 2 cut(s) 507, 657
Bst4CI ACNGT 2 cut(s) 163, 190
Bst6I CTCTTC 1 cut(s) 36
BstACI GRCGYC 3 cut(s) 197, 218, 282
BstBAI YACGTR 1 cut(s) 123
BstBI TTCGAA 1 cut(s) 65
BstC8I GCNNGC 6 cut(s) 84, 101, 294, 512, 516, 589
BstDEI CTNAG 1 cut(s) 275
BstF5I GGATG 1 cut(s) 675
BstH2I RGCGCY 1 cut(s) 221
BstHHI GCGC 2 cut(s) 220, 389
BstKTI GATC 3 cut(s) 117, 340, 699
BstMAI GTCTC 2 cut(s) 13, 397
BstMBI GATC 3 cut(s) 114, 337, 696
BstMCI CGRYCG 1 cut(s) 699
BstMWI GCNNNNNNNGC 8 cut(s) 263, 302, 311, 317, 395, 428, 536, 545
BstNI CCWGG 2 cut(s) 507, 657
BstSCI CCNGG 3 cut(s) 505, 518, 655
BstSNI TACGTA 1 cut(s) 123
BstV1I GCAGC 5 cut(s) 283, 415, 457, 652, 674
BstV2I GAAGAC 1 cut(s) 490
BstX2I RGATCY 1 cut(s) 114
BstYI RGATCY 1 cut(s) 114
Bsu15I ATCGAT 1 cut(s) 695
BsuRI GGCC 2 cut(s) 292, 504
BsuTUI ATCGAT 1 cut(s) 695
BtgZI GCGATG 1 cut(s) 529
BtsCI GGATG 1 cut(s) 675
Cac8I GCNNGC 6 cut(s) 84, 101, 294, 512, 516, 589
CfoI GCGC 2 cut(s) 220, 389
Cfr10I RCCGGY 2 cut(s) 99, 171
Cfr13I GGNCC 1 cut(s) 503
ClaI ATCGAT 1 cut(s) 695
CseI GACGC 1 cut(s) 186
Csp6I GTAC 2 cut(s) 186, 643
CviAII CATG 1 cut(s) 721
CviQI GTAC 2 cut(s) 186, 643
DdeI CTNAG 1 cut(s) 275
DinI GGCGCC 1 cut(s) 219
DpnI GATC 3 cut(s) 116, 339, 698
DpnII GATC 3 cut(s) 114, 337, 696
DrdI GACNNNNNNGTC 1 cut(s) 220
DseDI GACNNNNNNGTC 1 cut(s) 220
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
EciI GGCGGA 1 cut(s) 385
Eco105I TACGTA 1 cut(s) 123
Eco31I GGTCTC 1 cut(s) 13
Eco88I CYCGRG 1 cut(s) 110
EcoRII CCWGG 2 cut(s) 505, 655
EgeI GGCGCC 1 cut(s) 219
EheI GGCGCC 1 cut(s) 219
FaeI CATG 1 cut(s) 724
FaiI YATR 8 cut(s) 126, 287, 289, 446, 554, 608, 703, 722
FatI CATG 1 cut(s) 720
FauI CCCGC 1 cut(s) 196
FauNDI CATATG 1 cut(s) 287
FokI GGATG 1 cut(s) 662
GlaI GCGC 2 cut(s) 219, 388
GsuI CTGGAG 1 cut(s) 321
HaeII RGCGCY 1 cut(s) 221
HaeIII GGCC 2 cut(s) 292, 504
HapII CCGG 6 cut(s) 14, 40, 100, 155, 172, 520
HgaI GACGC 1 cut(s) 186
HhaI GCGC 2 cut(s) 220, 389
Hin1I GRCGYC 3 cut(s) 197, 218, 282
Hin1II CATG 1 cut(s) 724
Hin6I GCGC 2 cut(s) 218, 387
HinP1I GCGC 2 cut(s) 218, 387
HinfI GANTC 1 cut(s) 230
HpaII CCGG 6 cut(s) 14, 40, 100, 155, 172, 520
HphI GGTGA 4 cut(s) 28, 40, 403, 566
Hpy166II GTNNAC 1 cut(s) 166
Hpy188I TCNGA 2 cut(s) 354, 435
Hpy188III TCNNGA 1 cut(s) 112
Hpy8I GTNNAC 1 cut(s) 166
Hpy99I CGWCG 4 cut(s) 58, 61, 202, 226
HpyAV CCTTC 3 cut(s) 426, 695, 706
HpyCH4III ACNGT 2 cut(s) 163, 190
HpyCH4IV ACGT 3 cut(s) 90, 122, 282
HpyCH4V TGCA 2 cut(s) 377, 662
HpyF10VI GCNNNNNNNGC 8 cut(s) 263, 302, 311, 317, 395, 428, 536, 545
HpyF3I CTNAG 1 cut(s) 275
HpySE526I ACGT 3 cut(s) 90, 122, 282
Hsp92I GRCGYC 3 cut(s) 197, 218, 282
Hsp92II CATG 1 cut(s) 724
HspAI GCGC 2 cut(s) 218, 387
KasI GGCGCC 1 cut(s) 217
KroI GCCGGC 1 cut(s) 99
KroNI GCCGGC 1 cut(s) 101
Kzo9I GATC 3 cut(s) 114, 337, 696
LmnI GCTCC 3 cut(s) 302, 427, 614
Lsp1109I GCAGC 5 cut(s) 283, 415, 457, 652, 674
MaeII ACGT 3 cut(s) 90, 122, 282
MaeIII GTNAC 2 cut(s) 278, 704
MalI GATC 3 cut(s) 116, 339, 698
MboI GATC 3 cut(s) 114, 337, 696
MboII GAAGA 4 cut(s) 23, 26, 342, 490
MflI RGATCY 1 cut(s) 114
MluCI AATT 3 cut(s) 131, 634, 725
Mly113I GGCGCC 1 cut(s) 218
MnlI CCTC 6 cut(s) 33, 39, 61, 542, 588, 689
MroNI GCCGGC 1 cut(s) 99
MslI CAYNNNNRTG 1 cut(s) 365
MspA1I CMGCKG 1 cut(s) 665
MspI CCGG 6 cut(s) 14, 40, 100, 155, 172, 520
MspR9I CCNGG 3 cut(s) 507, 520, 657
Mva1269I GAATGC 1 cut(s) 103
MvaI CCWGG 2 cut(s) 507, 657
MwoI GCNNNNNNNGC 8 cut(s) 263, 302, 311, 317, 395, 428, 536, 545
NaeI GCCGGC 1 cut(s) 101
NarI GGCGCC 1 cut(s) 218
NciI CCSGG 1 cut(s) 520
NdeI CATATG 1 cut(s) 287
NdeII GATC 3 cut(s) 114, 337, 696
NgoMIV GCCGGC 1 cut(s) 99
NlaIII CATG 1 cut(s) 724
NlaIV GGNNCC 1 cut(s) 219
NmuCI GTSAC 1 cut(s) 278
NspV TTCGAA 1 cut(s) 65
PcsI WCGNNNNNNNCGW 4 cut(s) 62, 194, 221, 230
PctI GAATGC 1 cut(s) 103
PdiI GCCGGC 1 cut(s) 101
PfeI GAWTC 1 cut(s) 230
Ple19I CGATCG 1 cut(s) 699
PluTI GGCGCC 1 cut(s) 221
Ppu21I YACGTR 1 cut(s) 123
Psp1406I AACGTT 1 cut(s) 90
Psp6I CCWGG 2 cut(s) 505, 655
PspGI CCWGG 2 cut(s) 505, 655
PspN4I GGNNCC 1 cut(s) 219
PspPI GGNCC 1 cut(s) 503
PsrI GAACNNNNNNTAC 2 cut(s) 635, 667
PsuI RGATCY 1 cut(s) 114
PvuI CGATCG 1 cut(s) 699
PvuII CAGCTG 1 cut(s) 665
RsaI GTAC 2 cut(s) 187, 644
RsaNI GTAC 2 cut(s) 186, 643
RseI CAYNNNNRTG 1 cut(s) 365
Sau3AI GATC 3 cut(s) 114, 337, 696
Sau96I GGNCC 1 cut(s) 503
ScrFI CCNGG 3 cut(s) 507, 520, 657
SfoI GGCGCC 1 cut(s) 219
SfuI TTCGAA 1 cut(s) 65
SgrAI CRCCGGYG 1 cut(s) 171
SmiMI CAYNNNNRTG 1 cut(s) 365
SnaBI TACGTA 1 cut(s) 123
Sse9I AATT 3 cut(s) 131, 634, 725
SsiI CCGC 8 cut(s) 175, 203, 254, 257, 311, 396, 537, 584
SspDI GGCGCC 1 cut(s) 217
StyD4I CCNGG 3 cut(s) 505, 518, 655
TaaI ACNGT 2 cut(s) 163, 190
TaiI ACGT 3 cut(s) 93, 125, 285
TaqI TCGA 4 cut(s) 53, 65, 95, 695
TasI AATT 3 cut(s) 131, 634, 725
TauI GCSGC 6 cut(s) 178, 257, 260, 314, 539, 587
TfiI GAWTC 1 cut(s) 230
TseFI GTSAC 1 cut(s) 278
TseI GCWGC 5 cut(s) 296, 428, 470, 662, 665
Tsp45I GTSAC 1 cut(s) 278
TspDTI ATGAA 3 cut(s) 144, 397, 595
TspGWI ACGGA 1 cut(s) 484
XapI RAATTY 3 cut(s) 131, 634, 725
XcmI CCANNNNNNNNNTGG 1 cut(s) 550
ZraI GACGTC 1 cut(s) 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.