MD06G1187500.v1.1

HPP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007927
Physical Location & Seq
Forward (+)
32488864 .. 32501795
12932 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1187500.v1.1.491

Sequence Viewer

Length: 687 bp
ATGGGCATGCAGCTGATGAGGGCTTCTAGTTTTCATCACCATCTTCTTCATCCCAAAAGCACTTCTACCCCTCCCGCAAAAGCACTTTTTGGTTTAGAACATGTCAACAGCAGAAGCATAGTAAACATCAAAAGAAGAAATCAATATTATGGGATTGTGGCTTCAAGCAATTTTGCTTCATCACTTTGGGACAGTTGGAGGCCTGAGAAGGGTTCGGCTGCTCCTTCTCTCAGTGATATTATCTGGCCTTCTGCAGGGGCTTTTGCAGCAATGGCGTTATTGGGAAGGATGGATCAGATATTAGCACCAAAAGGAATTTCAATGACAATTGCACCATTAGGAGCTGTTTCTGCAGTCCTCTTTGCCACTCCTTCTGCTCCTGGTGCTCGGAAGTACAATATGTTCATGGCACAAATAGGTTGTGCAGCCATTGGGGTGCTTGCATTTTTCACATTTGGCCCTGGATGGCCTGCTAGAAGCTTTGCCCTAGCTGCATCCGTTGCTTTCATGATTTGCACACGTTCCACACATCCACCTGCTGCAAGCTTGCCTATACTCTTCATTGATGGAGCTAAGTTCCATAACCTGAGTTTTTGGTATGCTTTGTTCCCCGGCGCCGCCGGATGCCTTCTTCTTTGCTTGATTCAAGAGATAGTGTTATACTTGAAGGACAACTTCAAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HPP

Protein Analysis

229

Amino Acids

24.58

Weight (kDa)

9.81

Isoelectric Point (pI)

42.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TM_HPP PF04982 74 - 219 1.8e-29 HPP, transmembrane region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 544
Acc36I ACCTGC 1 cut(s) 544
AccB1I GGYRCC 1 cut(s) 614
AciI CCGC 2 cut(s) 75, 618
AclWI GGATC 1 cut(s) 300
AcsI RAATTY 2 cut(s) 315, 680
AcyI GRCGYC 1 cut(s) 615
AfaI GTAC 1 cut(s) 395
AfiI CCNNNNNNNGG 2 cut(s) 209, 254
AflIII ACRYGT 2 cut(s) 100, 518
AgsI TTSAA 5 cut(s) 165, 321, 647, 667, 679
AjnI CCWGG 2 cut(s) 379, 460
AjuI GAANNNNNNNTTGG 2 cut(s) 301, 333
AluBI AGCT 6 cut(s) 13, 344, 480, 491, 546, 572
AluI AGCT 6 cut(s) 13, 344, 480, 491, 546, 572
Alw21I GWGCWC 1 cut(s) 388
AlwI GGATC 1 cut(s) 300
AoxI GGCC 4 cut(s) 200, 245, 457, 467
ApeKI GCWGC 6 cut(s) 10, 218, 266, 425, 491, 539
ApoI RAATTY 2 cut(s) 315, 680
AspLEI GCGC 1 cut(s) 617
AspS9I GGNCC 1 cut(s) 458
AsuC2I CCSGG 1 cut(s) 612
AsuHPI GGTGA 1 cut(s) 29
BanI GGYRCC 1 cut(s) 614
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 6 cut(s) 22, 205, 278, 437, 478, 526
BccI CCATC 4 cut(s) 48, 283, 459, 560
BciT130I CCWGG 2 cut(s) 381, 462
BcnI CCSGG 1 cut(s) 612
BfaI CTAG 3 cut(s) 27, 474, 488
BfmI CTRYAG 2 cut(s) 252, 351
BfoI RGCGCY 1 cut(s) 618
BfuAI ACCTGC 1 cut(s) 544
BisI GCNGC 7 cut(s) 11, 219, 267, 426, 492, 540, 618
BlsI GCNGC 7 cut(s) 12, 220, 268, 427, 493, 541, 619
Bme1390I CCNGG 3 cut(s) 381, 462, 612
BmgT120I GGNCC 1 cut(s) 458
BmiI GGNNCC 1 cut(s) 616
BmrFI CCNGG 3 cut(s) 381, 462, 612
BmsI GCATC 2 cut(s) 503, 614
BpuMI CCSGG 1 cut(s) 612
BsaHI GRCGYC 1 cut(s) 615
BsaJI CCNNGG 2 cut(s) 460, 610
Bsc4I CCNNNNNNNGG 2 cut(s) 209, 254
Bse3DI GCAATG 1 cut(s) 276
BseBI CCWGG 2 cut(s) 381, 462
BseDI CCNNGG 2 cut(s) 460, 610
BseGI GGATG 6 cut(s) 49, 294, 470, 494, 529, 629
BseLI CCNNNNNNNGG 2 cut(s) 209, 254
BseMI GCAATG 1 cut(s) 276
BseMII CTCAG 3 cut(s) 195, 244, 578
BseXI GCAGC 6 cut(s) 22, 205, 278, 437, 478, 526
BsgI GTGCAG 1 cut(s) 444
BshFI GGCC 4 cut(s) 202, 247, 459, 469
BshNI GGYRCC 1 cut(s) 614
BsiHKAI GWGCWC 1 cut(s) 388
BsiSI CCGG 2 cut(s) 612, 621
BslFI GGGAC 1 cut(s) 203
BslI CCNNNNNNNGG 2 cut(s) 209, 254
BsmFI GGGAC 1 cut(s) 203
BsnI GGCC 4 cut(s) 202, 247, 459, 469
Bsp1286I GDGCHC 1 cut(s) 388
Bsp143I GATC 1 cut(s) 292
BspACI CCGC 2 cut(s) 75, 618
BspANI GGCC 4 cut(s) 202, 247, 459, 469
BspCNI CTCAG 3 cut(s) 196, 243, 579
BspHI TCATGA 1 cut(s) 507
BspLI GGNNCC 1 cut(s) 616
BspMAI CTGCAG 2 cut(s) 256, 355
BspMI ACCTGC 1 cut(s) 544
BspPI GGATC 1 cut(s) 300
BspT107I GGYRCC 1 cut(s) 614
BsrDI GCAATG 1 cut(s) 276
BssECI CCNNGG 2 cut(s) 460, 610
BssMI GATC 1 cut(s) 292
BssNI GRCGYC 1 cut(s) 615
Bst2UI CCWGG 2 cut(s) 381, 462
Bst4CI ACNGT 1 cut(s) 194
Bst6I CTCTTC 1 cut(s) 563
BstACI GRCGYC 1 cut(s) 615
BstAPI GCANNNNNTGC 1 cut(s) 500
BstC8I GCNNGC 5 cut(s) 8, 441, 471, 544, 548
BstDEI CTNAG 4 cut(s) 204, 230, 573, 587
BstENI CCTNNNNNAGG 1 cut(s) 252
BstF5I GGATG 6 cut(s) 49, 294, 470, 494, 529, 629
BstH2I RGCGCY 1 cut(s) 618
BstHHI GCGC 1 cut(s) 617
BstKTI GATC 1 cut(s) 295
BstMBI GATC 1 cut(s) 292
BstMWI GCNNNNNNNGC 6 cut(s) 266, 272, 350, 383, 491, 500
BstNI CCWGG 2 cut(s) 381, 462
BstNSI RCATGY 2 cut(s) 10, 104
BstSCI CCNGG 3 cut(s) 379, 460, 610
BstSFI CTRYAG 2 cut(s) 252, 351
BstV1I GCAGC 6 cut(s) 22, 205, 278, 437, 478, 526
BsuRI GGCC 4 cut(s) 202, 247, 459, 469
BtsCI GGATG 6 cut(s) 49, 294, 470, 494, 529, 629
BtsIMutI CAGTG 1 cut(s) 238
BveI ACCTGC 1 cut(s) 544
Cac8I GCNNGC 5 cut(s) 8, 441, 471, 544, 548
CciI TCATGA 1 cut(s) 507
CfoI GCGC 1 cut(s) 617
Cfr13I GGNCC 1 cut(s) 458
Csp6I GTAC 1 cut(s) 394
CviAII CATG 4 cut(s) 7, 101, 406, 508
CviQI GTAC 1 cut(s) 394
DdeI CTNAG 4 cut(s) 204, 230, 573, 587
DinI GGCGCC 1 cut(s) 616
DpnI GATC 1 cut(s) 294
DpnII GATC 1 cut(s) 292
Eam1104I CTCTTC 1 cut(s) 563
EarI CTCTTC 1 cut(s) 563
Eco147I AGGCCT 1 cut(s) 202
EcoNI CCTNNNNNAGG 1 cut(s) 252
EcoRII CCWGG 2 cut(s) 379, 460
EgeI GGCGCC 1 cut(s) 616
EheI GGCGCC 1 cut(s) 616
FaeI CATG 4 cut(s) 10, 104, 409, 511
FalI AAGNNNNNCTT 1 cut(s) 659
FaqI GGGAC 1 cut(s) 203
FatI CATG 4 cut(s) 6, 100, 405, 507
FauI CCCGC 1 cut(s) 82
Fnu4HI GCNGC 7 cut(s) 11, 219, 267, 426, 492, 540, 618
FokI GGATG 6 cut(s) 36, 301, 477, 481, 516, 636
Fsp4HI GCNGC 7 cut(s) 11, 219, 267, 426, 492, 540, 618
FspBI CTAG 3 cut(s) 27, 474, 488
GlaI GCGC 1 cut(s) 616
GluI GCNGC 7 cut(s) 11, 219, 267, 426, 492, 540, 618
HaeII RGCGCY 1 cut(s) 618
HaeIII GGCC 4 cut(s) 202, 247, 459, 469
HapII CCGG 2 cut(s) 612, 621
HhaI GCGC 1 cut(s) 617
Hin1I GRCGYC 1 cut(s) 615
Hin1II CATG 4 cut(s) 10, 104, 409, 511
Hin6I GCGC 1 cut(s) 615
HinP1I GCGC 1 cut(s) 615
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HindIII AAGCTT 2 cut(s) 478, 544
HinfI GANTC 1 cut(s) 643
HpaII CCGG 2 cut(s) 612, 621
HphI GGTGA 1 cut(s) 29
Hpy166II GTNNAC 2 cut(s) 106, 124
Hpy188I TCNGA 2 cut(s) 297, 390
Hpy188III TCNNGA 2 cut(s) 508, 647
Hpy8I GTNNAC 2 cut(s) 106, 124
HpyAV CCTTC 7 cut(s) 202, 234, 258, 279, 381, 638, 661
HpyCH4III ACNGT 1 cut(s) 194
HpyCH4IV ACGT 1 cut(s) 520
HpyF10VI GCNNNNNNNGC 6 cut(s) 266, 272, 350, 383, 491, 500
HpyF3I CTNAG 4 cut(s) 204, 230, 573, 587
HpySE526I ACGT 1 cut(s) 520
Hsp92I GRCGYC 1 cut(s) 615
Hsp92II CATG 4 cut(s) 10, 104, 409, 511
HspAI GCGC 1 cut(s) 615
KasI GGCGCC 1 cut(s) 614
Kzo9I GATC 1 cut(s) 292
LmnI GCTCC 4 cut(s) 226, 341, 382, 569
Lsp1109I GCAGC 6 cut(s) 22, 205, 278, 437, 478, 526
LweI GCATC 2 cut(s) 503, 614
MaeI CTAG 3 cut(s) 27, 474, 488
MaeII ACGT 1 cut(s) 520
MalI GATC 1 cut(s) 294
MboI GATC 1 cut(s) 292
MboII GAAGA 5 cut(s) 35, 38, 147, 550, 623
MfeI CAATTG 1 cut(s) 327
MhlI GDGCHC 1 cut(s) 388
MluCI AATT 4 cut(s) 169, 315, 327, 680
Mly113I GGCGCC 1 cut(s) 615
MmeI TCCRAC 1 cut(s) 176
MnlI CCTC 4 cut(s) 12, 81, 192, 368
MslI CAYNNNNRTG 1 cut(s) 434
MspA1I CMGCKG 1 cut(s) 13
MspI CCGG 2 cut(s) 612, 621
MspR9I CCNGG 3 cut(s) 381, 462, 612
MunI CAATTG 1 cut(s) 327
MvaI CCWGG 2 cut(s) 381, 462
MwoI GCNNNNNNNGC 6 cut(s) 266, 272, 350, 383, 491, 500
NarI GGCGCC 1 cut(s) 615
NciI CCSGG 1 cut(s) 612
NdeII GATC 1 cut(s) 292
NlaIII CATG 4 cut(s) 10, 104, 409, 511
NlaIV GGNNCC 1 cut(s) 616
NspI RCATGY 2 cut(s) 10, 104
PaeI GCATGC 1 cut(s) 10
PagI TCATGA 1 cut(s) 507
PaqCI CACCTGC 1 cut(s) 544
PceI AGGCCT 1 cut(s) 202
PciI ACATGT 1 cut(s) 100
PfeI GAWTC 1 cut(s) 643
PkrI GCNGC 7 cut(s) 12, 220, 268, 427, 493, 541, 619
PluTI GGCGCC 1 cut(s) 618
PscI ACATGT 1 cut(s) 100
Psp6I CCWGG 2 cut(s) 379, 460
PspGI CCWGG 2 cut(s) 379, 460
PspN4I GGNNCC 1 cut(s) 616
PspPI GGNCC 1 cut(s) 458
PsrI GAACNNNNNNTAC 4 cut(s) 386, 418, 590, 622
PstI CTGCAG 2 cut(s) 256, 355
PvuII CAGCTG 1 cut(s) 13
RsaI GTAC 1 cut(s) 395
RsaNI GTAC 1 cut(s) 394
RseI CAYNNNNRTG 1 cut(s) 434
SatI GCNGC 7 cut(s) 11, 219, 267, 426, 492, 540, 618
Sau3AI GATC 1 cut(s) 292
Sau96I GGNCC 1 cut(s) 458
ScrFI CCNGG 3 cut(s) 381, 462, 612
SduI GDGCHC 1 cut(s) 388
SfaNI GCATC 2 cut(s) 503, 614
SfcI CTRYAG 2 cut(s) 252, 351
SfoI GGCGCC 1 cut(s) 616
SmiMI CAYNNNNRTG 1 cut(s) 434
SphI GCATGC 1 cut(s) 10
Sse9I AATT 4 cut(s) 169, 315, 327, 680
SseBI AGGCCT 1 cut(s) 202
SsiI CCGC 2 cut(s) 75, 618
SspDI GGCGCC 1 cut(s) 614
SspI AATATT 1 cut(s) 146
SspMI CTAG 3 cut(s) 27, 474, 488
StuI AGGCCT 1 cut(s) 202
StyD4I CCNGG 3 cut(s) 379, 460, 610
TaaI ACNGT 1 cut(s) 194
TaiI ACGT 1 cut(s) 523
TasI AATT 4 cut(s) 169, 315, 327, 680
TatI WGTACW 1 cut(s) 393
TauI GCSGC 1 cut(s) 620
TfiI GAWTC 1 cut(s) 643
TscAI CASTG 1 cut(s) 238
TseI GCWGC 6 cut(s) 10, 218, 266, 425, 491, 539
TspDTI ATGAA 6 cut(s) 23, 38, 168, 394, 496, 550
TspGWI ACGGA 1 cut(s) 487
TspRI CASTG 1 cut(s) 238
XagI CCTNNNNNAGG 1 cut(s) 252
XapI RAATTY 2 cut(s) 315, 680
XceI RCATGY 2 cut(s) 10, 104
XspI CTAG 3 cut(s) 27, 474, 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.