MD14G1193900.v1.1

HPP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007927
Physical Location & Seq
Forward (+)
28500814 .. 28503574
2761 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1193900.v1.1.491

Sequence Viewer

Length: 687 bp
ATGGGCATGCAGCTGATGAGGACTTCTAGATTTCATCACCATCTTCTTCATCCCGAAAGCACTTCTACCCCTCCCACAAAAGCACTTTTTGGTTTAGGACATGTCAACAGCAGAAGCATAGTAAACATCAAAAGAAGAAATCAAATTTATGGGATTGTGGCTTCAAGCAATTTGGCTTCACCACTATGGGACAGCTGGAAGCCTGAGAAGGGTTCCGCTGCTCCTTCTCTCAGTGACATTACCTGGCCTTCTGCAGGGGCTTTTGCAGCAATGGCAATATTGGGGAGGATGGATCAGATATTAGCACAAAAAGGAATGTCAATGACAATTGCACCTTTAGGGGCAGTTTCTGCAGTCCTCTTTGCCACTCCATCCTCTCCTGGTGCTCGGAAGTACAATATGTTCATGGCACAAATAGGTTGTGCAGCAATTGGTGTACTGACATTTTCCACATTTGGCCCAGGATGGCTTGCTAGGAGCGTTGCCCTAGCTGCATCCGTTGCTTTCATGATTTGCACACGTTCCGCACATCCACCTGCTGCAAGCTTGCCTTTACTCTTCATCGATGGAGCGAAGTTCCATAACTTGAGTTTCTGGTATGCTTTGTTCCCGGGCACCGCTGGATGCGTTCTTCTTTGCTTGATTCAAGAAATAGTGTTATACTTGAAGGAAAACTTCAAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HPP

Protein Analysis

229

Amino Acids

24.53

Weight (kDa)

9.86

Isoelectric Point (pI)

45.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TM_HPP PF04982 74 - 221 1.7e-31 HPP, transmembrane region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 544
Acc36I ACCTGC 1 cut(s) 544
AccB1I GGYRCC 1 cut(s) 614
AciI CCGC 3 cut(s) 216, 525, 618
AclWI GGATC 1 cut(s) 300
AcsI RAATTY 2 cut(s) 144, 680
AfaI GTAC 2 cut(s) 395, 438
AfiI CCNNNNNNNGG 2 cut(s) 209, 254
AflIII ACRYGT 2 cut(s) 100, 518
AgsI TTSAA 4 cut(s) 165, 647, 667, 679
AjnI CCWGG 3 cut(s) 242, 379, 460
AluBI AGCT 4 cut(s) 13, 195, 491, 546
AluI AGCT 4 cut(s) 13, 195, 491, 546
Alw21I GWGCWC 1 cut(s) 388
AlwI GGATC 1 cut(s) 300
AlwNI CAGNNNCTG 1 cut(s) 350
Ama87I CYCGRG 1 cut(s) 610
AoxI GGCC 2 cut(s) 245, 457
ApeKI GCWGC 6 cut(s) 10, 218, 266, 425, 491, 539
ApoI RAATTY 2 cut(s) 144, 680
AspS9I GGNCC 1 cut(s) 458
AsuC2I CCSGG 2 cut(s) 611, 612
AsuHPI GGTGA 2 cut(s) 29, 171
AvaI CYCGRG 1 cut(s) 610
BaeGI GKGCMC 1 cut(s) 617
BanI GGYRCC 1 cut(s) 614
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 6 cut(s) 22, 205, 278, 437, 478, 526
BccI CCATC 5 cut(s) 48, 283, 379, 459, 560
BciT130I CCWGG 3 cut(s) 244, 381, 462
BcnI CCSGG 2 cut(s) 611, 612
BfaI CTAG 3 cut(s) 27, 474, 488
BfmI CTRYAG 2 cut(s) 252, 351
BfuAI ACCTGC 1 cut(s) 544
BisI GCNGC 6 cut(s) 11, 219, 267, 426, 492, 540
BlsI GCNGC 6 cut(s) 12, 220, 268, 427, 493, 541
Bme1390I CCNGG 5 cut(s) 244, 381, 462, 611, 612
BmeT110I CYCGRG 1 cut(s) 610
BmgT120I GGNCC 1 cut(s) 458
BmiI GGNNCC 2 cut(s) 214, 616
BmrFI CCNGG 5 cut(s) 244, 381, 462, 611, 612
BmsI GCATC 2 cut(s) 503, 614
BpuEI CTTGAG 1 cut(s) 607
BpuMI CCSGG 2 cut(s) 611, 612
Bsa29I ATCGAT 1 cut(s) 564
BsaJI CCNNGG 2 cut(s) 460, 610
Bsc4I CCNNNNNNNGG 2 cut(s) 209, 254
Bse3DI GCAATG 1 cut(s) 276
BseBI CCWGG 3 cut(s) 244, 381, 462
BseCI ATCGAT 1 cut(s) 564
BseDI CCNNGG 2 cut(s) 460, 610
BseGI GGATG 7 cut(s) 49, 294, 371, 470, 494, 529, 629
BseLI CCNNNNNNNGG 2 cut(s) 209, 254
BseMI GCAATG 1 cut(s) 276
BseMII CTCAG 2 cut(s) 195, 244
BseSI GKGCMC 1 cut(s) 617
BseXI GCAGC 6 cut(s) 22, 205, 278, 437, 478, 526
BsgI GTGCAG 1 cut(s) 444
BshFI GGCC 2 cut(s) 247, 459
BshNI GGYRCC 1 cut(s) 614
BshVI ATCGAT 1 cut(s) 564
BsiHKAI GWGCWC 1 cut(s) 388
BsiHKCI CYCGRG 1 cut(s) 610
BsiSI CCGG 1 cut(s) 611
BslFI GGGAC 1 cut(s) 203
BslI CCNNNNNNNGG 2 cut(s) 209, 254
BsmFI GGGAC 1 cut(s) 203
BsnI GGCC 2 cut(s) 247, 459
BsoBI CYCGRG 1 cut(s) 610
Bsp1286I GDGCHC 2 cut(s) 388, 617
Bsp143I GATC 1 cut(s) 292
BspACI CCGC 3 cut(s) 216, 525, 618
BspANI GGCC 2 cut(s) 247, 459
BspCNI CTCAG 2 cut(s) 196, 243
BspDI ATCGAT 1 cut(s) 564
BspHI TCATGA 1 cut(s) 507
BspLI GGNNCC 2 cut(s) 214, 616
BspMAI CTGCAG 2 cut(s) 256, 355
BspMI ACCTGC 1 cut(s) 544
BspPI GGATC 1 cut(s) 300
BspT107I GGYRCC 1 cut(s) 614
BsrDI GCAATG 1 cut(s) 276
BssECI CCNNGG 2 cut(s) 460, 610
BssMI GATC 1 cut(s) 292
Bst2UI CCWGG 3 cut(s) 244, 381, 462
Bst6I CTCTTC 1 cut(s) 563
BstAPI GCANNNNNTGC 2 cut(s) 350, 500
BstC8I GCNNGC 4 cut(s) 8, 471, 544, 548
BstDEI CTNAG 2 cut(s) 204, 230
BstENI CCTNNNNNAGG 1 cut(s) 252
BstF5I GGATG 7 cut(s) 49, 294, 371, 470, 494, 529, 629
BstKTI GATC 1 cut(s) 295
BstMBI GATC 1 cut(s) 292
BstMWI GCNNNNNNNGC 5 cut(s) 266, 272, 350, 491, 500
BstNI CCWGG 3 cut(s) 244, 381, 462
BstNSI RCATGY 2 cut(s) 10, 104
BstSCI CCNGG 5 cut(s) 242, 379, 460, 609, 610
BstSFI CTRYAG 2 cut(s) 252, 351
BstSLI GKGCMC 1 cut(s) 617
BstV1I GCAGC 6 cut(s) 22, 205, 278, 437, 478, 526
Bsu15I ATCGAT 1 cut(s) 564
BsuRI GGCC 2 cut(s) 247, 459
BsuTUI ATCGAT 1 cut(s) 564
BtsCI GGATG 7 cut(s) 49, 294, 371, 470, 494, 529, 629
BtsIMutI CAGTG 1 cut(s) 238
BveI ACCTGC 1 cut(s) 544
Cac8I GCNNGC 4 cut(s) 8, 471, 544, 548
CaiI CAGNNNCTG 1 cut(s) 350
CciI TCATGA 1 cut(s) 507
Cfr13I GGNCC 1 cut(s) 458
Cfr9I CCCGGG 1 cut(s) 610
ClaI ATCGAT 1 cut(s) 564
Csp6I GTAC 2 cut(s) 394, 437
CviAII CATG 4 cut(s) 7, 101, 406, 508
CviQI GTAC 2 cut(s) 394, 437
DdeI CTNAG 2 cut(s) 204, 230
DpnI GATC 1 cut(s) 294
DpnII GATC 1 cut(s) 292
Eam1104I CTCTTC 1 cut(s) 563
EarI CTCTTC 1 cut(s) 563
Eco88I CYCGRG 1 cut(s) 610
EcoNI CCTNNNNNAGG 1 cut(s) 252
EcoRII CCWGG 3 cut(s) 242, 379, 460
FaeI CATG 4 cut(s) 10, 104, 409, 511
FalI AAGNNNNNCTT 3 cut(s) 535, 567, 659
FaqI GGGAC 1 cut(s) 203
FatI CATG 4 cut(s) 6, 100, 405, 507
Fnu4HI GCNGC 6 cut(s) 11, 219, 267, 426, 492, 540
FokI GGATG 7 cut(s) 36, 301, 358, 477, 481, 516, 636
Fsp4HI GCNGC 6 cut(s) 11, 219, 267, 426, 492, 540
FspBI CTAG 3 cut(s) 27, 474, 488
GluI GCNGC 6 cut(s) 11, 219, 267, 426, 492, 540
HaeIII GGCC 2 cut(s) 247, 459
HapII CCGG 1 cut(s) 611
Hin1II CATG 4 cut(s) 10, 104, 409, 511
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HindIII AAGCTT 1 cut(s) 544
HinfI GANTC 1 cut(s) 643
HpaII CCGG 1 cut(s) 611
HphI GGTGA 2 cut(s) 29, 171
Hpy166II GTNNAC 3 cut(s) 106, 124, 437
Hpy188I TCNGA 2 cut(s) 297, 390
Hpy188III TCNNGA 4 cut(s) 27, 53, 508, 647
Hpy8I GTNNAC 3 cut(s) 106, 124, 437
HpyAV CCTTC 4 cut(s) 202, 234, 258, 661
HpyCH4IV ACGT 1 cut(s) 520
HpyCH4V TGCA 9 cut(s) 10, 254, 266, 332, 353, 425, 494, 516, 542
HpyF10VI GCNNNNNNNGC 5 cut(s) 266, 272, 350, 491, 500
HpyF3I CTNAG 2 cut(s) 204, 230
HpySE526I ACGT 1 cut(s) 520
Hsp92II CATG 4 cut(s) 10, 104, 409, 511
Kzo9I GATC 1 cut(s) 292
LmnI GCTCC 3 cut(s) 226, 477, 569
Lsp1109I GCAGC 6 cut(s) 22, 205, 278, 437, 478, 526
LweI GCATC 2 cut(s) 503, 614
MaeI CTAG 3 cut(s) 27, 474, 488
MaeII ACGT 1 cut(s) 520
MaeIII GTNAC 1 cut(s) 233
MalI GATC 1 cut(s) 294
MboI GATC 1 cut(s) 292
MboII GAAGA 5 cut(s) 35, 38, 147, 550, 623
MfeI CAATTG 2 cut(s) 327, 429
MhlI GDGCHC 2 cut(s) 388, 617
MluCI AATT 5 cut(s) 144, 169, 327, 429, 680
MnlI CCTC 5 cut(s) 12, 81, 279, 368, 385
MslI CAYNNNNRTG 1 cut(s) 184
MspA1I CMGCKG 4 cut(s) 13, 195, 218, 620
MspI CCGG 1 cut(s) 611
MspR9I CCNGG 5 cut(s) 244, 381, 462, 611, 612
MunI CAATTG 2 cut(s) 327, 429
MvaI CCWGG 3 cut(s) 244, 381, 462
MwoI GCNNNNNNNGC 5 cut(s) 266, 272, 350, 491, 500
NciI CCSGG 2 cut(s) 611, 612
NdeII GATC 1 cut(s) 292
NlaIII CATG 4 cut(s) 10, 104, 409, 511
NlaIV GGNNCC 2 cut(s) 214, 616
NmuCI GTSAC 1 cut(s) 233
NspI RCATGY 2 cut(s) 10, 104
PaeI GCATGC 1 cut(s) 10
PagI TCATGA 1 cut(s) 507
PaqCI CACCTGC 1 cut(s) 544
PciI ACATGT 1 cut(s) 100
PfeI GAWTC 1 cut(s) 643
PkrI GCNGC 6 cut(s) 12, 220, 268, 427, 493, 541
PscI ACATGT 1 cut(s) 100
Psp6I CCWGG 3 cut(s) 242, 379, 460
PspGI CCWGG 3 cut(s) 242, 379, 460
PspN4I GGNNCC 2 cut(s) 214, 616
PspPI GGNCC 1 cut(s) 458
PsrI GAACNNNNNNTAC 4 cut(s) 386, 418, 590, 622
PstI CTGCAG 2 cut(s) 256, 355
PstNI CAGNNNCTG 1 cut(s) 350
PvuII CAGCTG 2 cut(s) 13, 195
RsaI GTAC 2 cut(s) 395, 438
RsaNI GTAC 2 cut(s) 394, 437
RseI CAYNNNNRTG 1 cut(s) 184
SatI GCNGC 6 cut(s) 11, 219, 267, 426, 492, 540
Sau3AI GATC 1 cut(s) 292
Sau96I GGNCC 1 cut(s) 458
ScrFI CCNGG 5 cut(s) 244, 381, 462, 611, 612
SduI GDGCHC 2 cut(s) 388, 617
SetI ASST 9 cut(s) 15, 197, 245, 337, 421, 493, 523, 538, 548
SfaNI GCATC 2 cut(s) 503, 614
SfcI CTRYAG 2 cut(s) 252, 351
SmaI CCCGGG 1 cut(s) 612
SmiMI CAYNNNNRTG 1 cut(s) 184
SmlI CTYRAG 1 cut(s) 586
SmoI CTYRAG 1 cut(s) 586
SphI GCATGC 1 cut(s) 10
Sse9I AATT 5 cut(s) 144, 169, 327, 429, 680
SsiI CCGC 3 cut(s) 216, 525, 618
SspI AATATT 1 cut(s) 279
SspMI CTAG 3 cut(s) 27, 474, 488
StyD4I CCNGG 5 cut(s) 242, 379, 460, 609, 610
TaiI ACGT 1 cut(s) 523
TaqI TCGA 1 cut(s) 564
TasI AATT 5 cut(s) 144, 169, 327, 429, 680
TatI WGTACW 2 cut(s) 393, 436
TfiI GAWTC 1 cut(s) 643
TscAI CASTG 1 cut(s) 238
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 6 cut(s) 10, 218, 266, 425, 491, 539
Tsp45I GTSAC 1 cut(s) 233
TspDTI ATGAA 5 cut(s) 23, 38, 394, 496, 550
TspGWI ACGGA 1 cut(s) 487
TspMI CCCGGG 1 cut(s) 610
TspRI CASTG 1 cut(s) 238
XagI CCTNNNNNAGG 1 cut(s) 252
XapI RAATTY 2 cut(s) 144, 680
XbaI TCTAGA 1 cut(s) 26
XceI RCATGY 2 cut(s) 10, 104
XmaI CCCGGG 1 cut(s) 610
XspI CTAG 3 cut(s) 27, 474, 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.