AT5G62930

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009216
Physical Location & Seq
Forward (+)
25254821 .. 25256619
1799 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G62930.2

Sequence Viewer

Length: 729 bp
ATGAGGCCGGAGATAGTTCTGTTCGGCGACTCGATCACGGCGCAGTCTTTTAGGTCCGGCGGTTGGGGATCTGCTCTTGCCGACGCTTACTCTCGCAAGGCTGATGTTGTGGTTCGAGGCTACGGCGGCTACAACACCCGATGGGCTCTCTTCTTGCTTCATCACATCTTCCCTCTCGGCTCTTCGTCTCCTCCTGTTGCTACGACGATATTCTTCGGTGCAAACGATGCAGCTCTCAAAGGAAGAACCAGTGATAGACAACATGTGCCGGTGGAAGAGTACACAGATAATGTCAGAAAGATTGTTCAGCATTTGAAGAAATGTTCACCTACAATGCTAATTGTGCTTATAACTCCACCACCAATTGATGAAGCTGGACGTCAAAGTTATGCAGAATCAATCTACGGTGAGAAAGCTATGAAAGAGCCTGAGAGAACAAACGAAACAACAGGGGTATATGCACAACATTGTGTTGCATTGGCCGAGGAACTCGGTCTGCGATGTGTCAACTTATGGTCTAAGATGCAGGAAACCAATGATTGGCAGAAAAAGTACCTAAGTGATGGGCTCCATCTCACGCCTGAAGGCAATGGGGTAGTTTTTGATGAAGTCTCGAGAGTTTTTAGAGAAGCTTGGCTCTCTCCCGAAGAAATGCCGTTTGATTTCCCCCATCATTCGCATATCGATGGTAAAAACCCATCGAAAGCTTTTGAAGAGCGTTGCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

242

Amino Acids

27.16

Weight (kDa)

5.99

Isoelectric Point (pI)

50.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 230 1.6e-33 GDSL-like Lipase/Acylhydrolase
Lipase_GDSL_2 PF13472 33 - 223 1e-24 GDSL-like Lipase/Acylhydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 350, 727
AatII GACGTC 1 cut(s) 382
AccB7I CCANNNNNTGG 1 cut(s) 540
AciI CCGC 2 cut(s) 60, 126
AclWI GGATC 1 cut(s) 76
AcoI YGGCCR 1 cut(s) 480
AcuI CTGAAG 1 cut(s) 603
AcyI GRCGYC 1 cut(s) 379
AfaI GTAC 2 cut(s) 281, 554
AfiI CCNNNNNNNGG 2 cut(s) 63, 540
AflIII ACRYGT 1 cut(s) 262
AgsI TTSAA 2 cut(s) 316, 713
AluBI AGCT 5 cut(s) 233, 374, 416, 632, 707
AluI AGCT 5 cut(s) 233, 374, 416, 632, 707
Alw26I GTCTC 2 cut(s) 192, 616
AlwI GGATC 1 cut(s) 76
Ama87I CYCGRG 1 cut(s) 613
AoxI GGCC 2 cut(s) 5, 480
ApeKI GCWGC 1 cut(s) 230
AspLEI GCGC 1 cut(s) 43
AspS9I GGNCC 1 cut(s) 54
AsuHPI GGTGA 2 cut(s) 318, 419
AvaI CYCGRG 1 cut(s) 613
AvaII GGWCC 1 cut(s) 54
BanII GRGCYC 2 cut(s) 148, 570
BbvI GCAGC 1 cut(s) 242
BccI CCATC 6 cut(s) 135, 557, 579, 678, 680, 706
BceAI ACGGC 3 cut(s) 54, 139, 640
BcoDI GTCTC 2 cut(s) 192, 616
BisI GCNGC 2 cut(s) 127, 231
BlsI GCNGC 2 cut(s) 128, 232
Bme18I GGWCC 1 cut(s) 54
BmeT110I CYCGRG 1 cut(s) 613
BmgT120I GGNCC 1 cut(s) 54
BmiI GGNNCC 1 cut(s) 569
BmsI GCATC 2 cut(s) 217, 513
Bsa29I ATCGAT 1 cut(s) 684
BsaHI GRCGYC 1 cut(s) 379
BsaJI CCNNGG 1 cut(s) 483
Bsc4I CCNNNNNNNGG 2 cut(s) 63, 540
Bse118I RCCGGY 1 cut(s) 268
Bse1I ACTGG 1 cut(s) 249
Bse3DI GCAATG 1 cut(s) 595
BseCI ATCGAT 1 cut(s) 684
BseDI CCNNGG 1 cut(s) 483
BseLI CCNNNNNNNGG 2 cut(s) 63, 540
BseMI GCAATG 1 cut(s) 595
BseMII CTCAG 1 cut(s) 420
BseNI ACTGG 1 cut(s) 249
BseRI GAGGAG 1 cut(s) 180
BseXI GCAGC 1 cut(s) 242
BshFI GGCC 2 cut(s) 7, 482
BshVI ATCGAT 1 cut(s) 684
BsiHKCI CYCGRG 1 cut(s) 613
BsiSI CCGG 3 cut(s) 8, 57, 269
BslI CCNNNNNNNGG 2 cut(s) 63, 540
BsmAI GTCTC 2 cut(s) 192, 616
BsmBI CGTCTC 1 cut(s) 192
BsnI GGCC 2 cut(s) 7, 482
BsoBI CYCGRG 1 cut(s) 613
Bsp1286I GDGCHC 2 cut(s) 148, 570
Bsp143I GATC 2 cut(s) 33, 68
BspACI CCGC 2 cut(s) 60, 126
BspANI GGCC 2 cut(s) 7, 482
BspCNI CTCAG 1 cut(s) 421
BspDI ATCGAT 1 cut(s) 684
BspLI GGNNCC 1 cut(s) 569
BspPI GGATC 1 cut(s) 76
BspQI GCTCTTC 2 cut(s) 187, 708
BsrDI GCAATG 1 cut(s) 595
BsrFI RCCGGY 1 cut(s) 268
BsrI ACTGG 1 cut(s) 249
BssAI RCCGGY 1 cut(s) 268
BssECI CCNNGG 1 cut(s) 483
BssMI GATC 2 cut(s) 33, 68
BssNI GRCGYC 1 cut(s) 379
Bst4CI ACNGT 1 cut(s) 407
Bst6I CTCTTC 4 cut(s) 155, 187, 270, 708
BstACI GRCGYC 1 cut(s) 379
BstAPI GCANNNNNTGC 1 cut(s) 227
BstDEI CTNAG 3 cut(s) 429, 519, 557
BstHHI GCGC 1 cut(s) 43
BstKTI GATC 2 cut(s) 36, 71
BstMAI GTCTC 2 cut(s) 192, 616
BstMBI GATC 2 cut(s) 33, 68
BstMWI GCNNNNNNNGC 3 cut(s) 126, 227, 343
BstNSI RCATGY 1 cut(s) 266
BstV1I GCAGC 1 cut(s) 242
BstX2I RGATCY 1 cut(s) 68
BstYI RGATCY 1 cut(s) 68
Bsu15I ATCGAT 1 cut(s) 684
BsuRI GGCC 2 cut(s) 7, 482
BsuTUI ATCGAT 1 cut(s) 684
BtgZI GCGATG 1 cut(s) 514
BtsIMutI CAGTG 1 cut(s) 256
CfoI GCGC 1 cut(s) 43
Cfr10I RCCGGY 1 cut(s) 268
Cfr13I GGNCC 1 cut(s) 54
ClaI ATCGAT 1 cut(s) 684
CseI GACGC 1 cut(s) 92
Csp6I GTAC 2 cut(s) 280, 553
CviAII CATG 1 cut(s) 263
CviQI GTAC 2 cut(s) 280, 553
DdeI CTNAG 3 cut(s) 429, 519, 557
DpnI GATC 2 cut(s) 35, 70
DpnII GATC 2 cut(s) 33, 68
EaeI YGGCCR 1 cut(s) 480
Eam1104I CTCTTC 4 cut(s) 155, 187, 270, 708
EarI CTCTTC 4 cut(s) 155, 187, 270, 708
Eco24I GRGCYC 2 cut(s) 148, 570
Eco47I GGWCC 1 cut(s) 54
Eco57I CTGAAG 1 cut(s) 603
Eco88I CYCGRG 1 cut(s) 613
EcoT38I GRGCYC 2 cut(s) 148, 570
Esp3I CGTCTC 1 cut(s) 192
FaeI CATG 1 cut(s) 266
FaiI YATR 9 cut(s) 264, 350, 390, 419, 457, 459, 514, 681, 727
FatI CATG 1 cut(s) 262
Fnu4HI GCNGC 2 cut(s) 127, 231
FriOI GRGCYC 2 cut(s) 148, 570
Fsp4HI GCNGC 2 cut(s) 127, 231
GlaI GCGC 1 cut(s) 42
GluI GCNGC 2 cut(s) 127, 231
HaeIII GGCC 2 cut(s) 7, 482
HapII CCGG 3 cut(s) 8, 57, 269
HgaI GACGC 1 cut(s) 92
HhaI GCGC 1 cut(s) 43
Hin1I GRCGYC 1 cut(s) 379
Hin1II CATG 1 cut(s) 266
Hin6I GCGC 1 cut(s) 41
HinP1I GCGC 1 cut(s) 41
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HindIII AAGCTT 2 cut(s) 630, 705
HinfI GANTC 2 cut(s) 29, 395
HpaII CCGG 3 cut(s) 8, 57, 269
HphI GGTGA 2 cut(s) 318, 419
Hpy166II GTNNAC 3 cut(s) 282, 326, 508
Hpy188I TCNGA 1 cut(s) 296
Hpy188III TCNNGA 3 cut(s) 613, 615, 644
Hpy8I GTNNAC 3 cut(s) 282, 326, 508
Hpy99I CGWCG 2 cut(s) 86, 208
HpyAV CCTTC 1 cut(s) 578
HpyCH4III ACNGT 1 cut(s) 407
HpyCH4IV ACGT 1 cut(s) 379
HpyCH4V TGCA 6 cut(s) 221, 230, 392, 461, 476, 526
HpyF10VI GCNNNNNNNGC 3 cut(s) 126, 227, 343
HpyF3I CTNAG 3 cut(s) 429, 519, 557
HpySE526I ACGT 1 cut(s) 379
Hsp92I GRCGYC 1 cut(s) 379
Hsp92II CATG 1 cut(s) 266
HspAI GCGC 1 cut(s) 41
Kzo9I GATC 2 cut(s) 33, 68
LguI GCTCTTC 2 cut(s) 187, 708
LmnI GCTCC 1 cut(s) 573
Lsp1109I GCAGC 1 cut(s) 242
LweI GCATC 2 cut(s) 217, 513
MaeII ACGT 1 cut(s) 379
MalI GATC 2 cut(s) 35, 70
MboI GATC 2 cut(s) 33, 68
MboII GAAGA 9 cut(s) 142, 160, 174, 205, 255, 287, 328, 659, 725
MfeI CAATTG 1 cut(s) 363
MflI RGATCY 1 cut(s) 68
MhlI GDGCHC 2 cut(s) 148, 570
MluCI AATT 2 cut(s) 339, 363
MlyI GAGTC 1 cut(s) 23
MnlI CCTC 4 cut(s) 110, 183, 201, 478
MslI CAYNNNNRTG 1 cut(s) 684
MspI CCGG 3 cut(s) 8, 57, 269
MunI CAATTG 1 cut(s) 363
MwoI GCNNNNNNNGC 3 cut(s) 126, 227, 343
NdeII GATC 2 cut(s) 33, 68
NlaIII CATG 1 cut(s) 266
NlaIV GGNNCC 1 cut(s) 569
NmeAIII GCCGAG 2 cut(s) 156, 508
NspI RCATGY 1 cut(s) 266
PaeR7I CTCGAG 1 cut(s) 613
PciI ACATGT 1 cut(s) 262
PciSI GCTCTTC 2 cut(s) 187, 708
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PfeI GAWTC 1 cut(s) 395
PflMI CCANNNNNTGG 1 cut(s) 540
PkrI GCNGC 2 cut(s) 128, 232
PleI GAGTC 1 cut(s) 23
PpsI GAGTC 1 cut(s) 23
PscI ACATGT 1 cut(s) 262
PsiI TTATAA 2 cut(s) 350, 727
PspN4I GGNNCC 1 cut(s) 569
PspPI GGNCC 1 cut(s) 54
PsuI RGATCY 1 cut(s) 68
RsaI GTAC 2 cut(s) 281, 554
RsaNI GTAC 2 cut(s) 280, 553
RseI CAYNNNNRTG 1 cut(s) 684
SapI GCTCTTC 2 cut(s) 187, 708
SatI GCNGC 2 cut(s) 127, 231
Sau3AI GATC 2 cut(s) 33, 68
Sau96I GGNCC 1 cut(s) 54
SchI GAGTC 1 cut(s) 23
SduI GDGCHC 2 cut(s) 148, 570
SetI ASST 9 cut(s) 56, 235, 331, 376, 382, 418, 558, 634, 709
SfaNI GCATC 2 cut(s) 217, 513
Sfr274I CTCGAG 1 cut(s) 613
SinI GGWCC 1 cut(s) 54
SlaI CTCGAG 1 cut(s) 613
SmiMI CAYNNNNRTG 1 cut(s) 684
SmlI CTYRAG 1 cut(s) 613
SmoI CTYRAG 1 cut(s) 613
Sse9I AATT 2 cut(s) 339, 363
SsiI CCGC 2 cut(s) 60, 126
TaaI ACNGT 1 cut(s) 407
TaiI ACGT 1 cut(s) 382
TaqI TCGA 5 cut(s) 32, 115, 614, 684, 701
TaqII GACCGA 1 cut(s) 482
TasI AATT 2 cut(s) 339, 363
TatI WGTACW 1 cut(s) 279
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 1 cut(s) 395
TscAI CASTG 1 cut(s) 256
TseI GCWGC 1 cut(s) 230
TspDTI ATGAA 4 cut(s) 149, 384, 434, 621
TspRI CASTG 1 cut(s) 256
Van91I CCANNNNNTGG 1 cut(s) 540
VpaK11BI GGWCC 1 cut(s) 54
XceI RCATGY 1 cut(s) 266
XhoI CTCGAG 1 cut(s) 613
ZraI GACGTC 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.