pycom14g14920

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0009216
Physical Location & Seq
Reverse (-)
17572233 .. 17574441
2209 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g14920.6

Sequence Viewer

Length: 729 bp
ATGAGGCCTGAGATTGTGTTGTTCGGAGACTCAATCACCCAACAATCCTTCCGATCAGGTGGCTGGGGCGCCGCTCTAGCTGATTCTTACTCCCGCAAGGCCGATGTAAAAGTTCGTGGGTATGGCGGGTACAACACGAGATGGGCGCTGTTCTTGCTGCAGCACCTCTTTCCTTTGGATTCTAAGAAACCTCCTGCTGCTGCCACAATTTTCTTTGGGGCCAATGACGCGGCTGTTTTAGGCAGAACTGGCGAACGGCAACATGTTCCCGTTGAAGAGTACAAGGAGAATCTCAGAAAAATTGTTCTTCATCTCAAGGAGTGTTCTCCCACCATTTTGATTGTGCTTATTACTCCACCACCTGTTGATGAGGATGGCCGTAATGAATTTGCACGATCTTTATATGGTAAGGATGCTAGTGGACTTCCAGAAAGGATGAATGAAGTGACAGGAGTTTATGCGAAGAAGTGCATTGAGCTGGCTGAGGAAATGGGTCTACGCTCCATCAATCTTTGGTCCGCAATGCAGGAAACAGAGGGTTGGCAGAAGAAATTTCTAAGTGATGGGTTGCACCTAACACCAGAAGGCAATGCTGTTTTCCACCAAGAAGTCGTCAAAGTTTTCAGCGAAGCATGGCTTTCTGCCGCGGAAATGCCTTATGATTTCCCTCACCACTCAGAAATTGATGGGAAGAACCCTGAGAAAGCTTTCCAGCAACGATGCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

243

Amino Acids

27.15

Weight (kDa)

6.0

Isoelectric Point (pI)

50.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 68
AccBSI CCGCTC 1 cut(s) 74
AccI GTMKAC 1 cut(s) 496
AccII CGCG 2 cut(s) 230, 647
AciI CCGC 7 cut(s) 72, 94, 126, 230, 519, 645, 647
AcoI YGGCCR 1 cut(s) 376
AcsI RAATTY 2 cut(s) 386, 551
AcyI GRCGYC 1 cut(s) 69
AfaI GTAC 2 cut(s) 131, 281
AflIII ACRYGT 1 cut(s) 262
AgsI TTSAA 1 cut(s) 275
AluBI AGCT 3 cut(s) 80, 478, 707
AluI AGCT 3 cut(s) 80, 478, 707
Alw26I GTCTC 1 cut(s) 21
AoxI GGCC 4 cut(s) 5, 99, 219, 376
ApeKI GCWGC 4 cut(s) 157, 160, 197, 200
ApoI RAATTY 2 cut(s) 386, 551
Asp700I GAANNNNTTC 1 cut(s) 707
AspLEI GCGC 2 cut(s) 71, 148
AspS9I GGNCC 2 cut(s) 219, 516
AsuHPI GGTGA 2 cut(s) 28, 662
AvaII GGWCC 1 cut(s) 516
BanI GGYRCC 1 cut(s) 68
BauI CACGAG 1 cut(s) 136
BbvCI CCTCAGC 1 cut(s) 483
BbvI GCAGC 4 cut(s) 144, 172, 184, 187
BccI CCATC 5 cut(s) 135, 368, 512, 557, 680
BceAI ACGGC 2 cut(s) 272, 363
BcoDI GTCTC 1 cut(s) 21
BfaI CTAG 2 cut(s) 77, 417
BfmI CTRYAG 1 cut(s) 158
BfoI RGCGCY 2 cut(s) 72, 149
BisI GCNGC 7 cut(s) 72, 158, 161, 198, 201, 231, 645
BlsI GCNGC 7 cut(s) 73, 159, 162, 199, 202, 232, 646
Bme18I GGWCC 1 cut(s) 516
BmgT120I GGNCC 2 cut(s) 219, 516
BmiI GGNNCC 2 cut(s) 70, 220
BmsI GCATC 2 cut(s) 403, 710
Bpu10I CCTNAGC 1 cut(s) 483
BpuEI CTTGAG 1 cut(s) 299
BsaHI GRCGYC 1 cut(s) 69
BsaJI CCNNGG 1 cut(s) 645
Bse1I ACTGG 1 cut(s) 253
Bse3DI GCAATG 2 cut(s) 528, 595
BseDI CCNNGG 1 cut(s) 645
BseGI GGATG 3 cut(s) 379, 418, 441
BseMI GCAATG 2 cut(s) 528, 595
BseMII CTCAG 4 cut(s) 307, 474, 690, 690
BseNI ACTGG 1 cut(s) 253
BseXI GCAGC 4 cut(s) 144, 172, 184, 187
BseYI CCCAGC 1 cut(s) 63
Bsh1236I CGCG 2 cut(s) 230, 647
BshFI GGCC 4 cut(s) 7, 101, 221, 378
BshNI GGYRCC 1 cut(s) 68
BsmAI GTCTC 1 cut(s) 21
BsnI GGCC 4 cut(s) 7, 101, 221, 378
Bsp143I GATC 2 cut(s) 53, 395
BspACI CCGC 7 cut(s) 72, 94, 126, 230, 519, 645, 647
BspANI GGCC 4 cut(s) 7, 101, 221, 378
BspCNI CTCAG 4 cut(s) 306, 475, 689, 691
BspFNI CGCG 2 cut(s) 230, 647
BspLI GGNNCC 2 cut(s) 70, 220
BspMAI CTGCAG 1 cut(s) 162
BspT107I GGYRCC 1 cut(s) 68
BsrBI CCGCTC 1 cut(s) 74
BsrDI GCAATG 2 cut(s) 528, 595
BsrI ACTGG 1 cut(s) 253
BssECI CCNNGG 1 cut(s) 645
BssMI GATC 2 cut(s) 53, 395
BssNI GRCGYC 1 cut(s) 69
BssSI CACGAG 1 cut(s) 136
Bst2BI CACGAG 1 cut(s) 136
Bst6I CTCTTC 1 cut(s) 270
BstACI GRCGYC 1 cut(s) 69
BstC8I GCNNGC 1 cut(s) 480
BstDEI CTNAG 7 cut(s) 9, 183, 293, 483, 557, 676, 699
BstDSI CCRYGG 1 cut(s) 645
BstF5I GGATG 3 cut(s) 379, 418, 441
BstFNI CGCG 2 cut(s) 230, 647
BstH2I RGCGCY 2 cut(s) 72, 149
BstHHI GCGC 2 cut(s) 71, 148
BstKTI GATC 2 cut(s) 56, 398
BstMAI GTCTC 1 cut(s) 21
BstMBI GATC 2 cut(s) 53, 395
BstMWI GCNNNNNNNGC 4 cut(s) 77, 154, 227, 249
BstNSI RCATGY 1 cut(s) 266
BstSFI CTRYAG 1 cut(s) 158
BstUI CGCG 2 cut(s) 230, 647
BstV1I GCAGC 4 cut(s) 144, 172, 184, 187
BsuRI GGCC 4 cut(s) 7, 101, 221, 378
BtgI CCRYGG 1 cut(s) 645
BtsCI GGATG 3 cut(s) 379, 418, 441
Cac8I GCNNGC 1 cut(s) 480
CfoI GCGC 2 cut(s) 71, 148
Cfr13I GGNCC 2 cut(s) 219, 516
Cfr42I CCGCGG 1 cut(s) 648
CseI GACGC 1 cut(s) 236
Csp6I GTAC 2 cut(s) 130, 280
CviAII CATG 2 cut(s) 263, 633
CviQI GTAC 2 cut(s) 130, 280
DdeI CTNAG 7 cut(s) 9, 183, 293, 483, 557, 676, 699
DinI GGCGCC 1 cut(s) 70
DpnI GATC 2 cut(s) 55, 397
DpnII GATC 2 cut(s) 53, 395
EaeI YGGCCR 1 cut(s) 376
Eam1104I CTCTTC 1 cut(s) 270
EarI CTCTTC 1 cut(s) 270
Eco147I AGGCCT 1 cut(s) 7
Eco47I GGWCC 1 cut(s) 516
EcoT22I ATGCAT 1 cut(s) 725
EgeI GGCGCC 1 cut(s) 70
EheI GGCGCC 1 cut(s) 70
FaeI CATG 2 cut(s) 266, 636
FaiI YATR 9 cut(s) 123, 264, 403, 405, 459, 634, 660, 725, 727
FalI AAGNNNNNCTT 2 cut(s) 621, 653
FatI CATG 2 cut(s) 262, 632
FauI CCCGC 2 cut(s) 101, 119
FblI GTMKAC 1 cut(s) 496
Fnu4HI GCNGC 7 cut(s) 72, 158, 161, 198, 201, 231, 645
FokI GGATG 3 cut(s) 386, 425, 448
Fsp4HI GCNGC 7 cut(s) 72, 158, 161, 198, 201, 231, 645
FspBI CTAG 2 cut(s) 77, 417
GlaI GCGC 2 cut(s) 70, 147
GluI GCNGC 7 cut(s) 72, 158, 161, 198, 201, 231, 645
GsaI CCCAGC 1 cut(s) 67
HaeII RGCGCY 2 cut(s) 72, 149
HaeIII GGCC 4 cut(s) 7, 101, 221, 378
HgaI GACGC 1 cut(s) 236
HhaI GCGC 2 cut(s) 71, 148
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 2 cut(s) 266, 636
Hin6I GCGC 2 cut(s) 69, 146
HinP1I GCGC 2 cut(s) 69, 146
HindIII AAGCTT 1 cut(s) 705
HinfI GANTC 4 cut(s) 29, 83, 179, 289
HphI GGTGA 2 cut(s) 28, 662
Hpy166II GTNNAC 2 cut(s) 422, 497
Hpy188I TCNGA 4 cut(s) 26, 53, 296, 679
Hpy188III TCNNGA 1 cut(s) 428
Hpy8I GTNNAC 2 cut(s) 422, 497
HpyAV CCTTC 2 cut(s) 58, 578
HpyCH4V TGCA 6 cut(s) 160, 392, 471, 526, 571, 723
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 154, 227, 249
HpyF3I CTNAG 7 cut(s) 9, 183, 293, 483, 557, 676, 699
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 2 cut(s) 266, 636
HspAI GCGC 2 cut(s) 69, 146
KasI GGCGCC 1 cut(s) 68
KspI CCGCGG 1 cut(s) 648
Kzo9I GATC 2 cut(s) 53, 395
LmnI GCTCC 1 cut(s) 506
Lsp1109I GCAGC 4 cut(s) 144, 172, 184, 187
LweI GCATC 2 cut(s) 403, 710
MaeI CTAG 2 cut(s) 77, 417
MaeIII GTNAC 1 cut(s) 445
MalI GATC 2 cut(s) 55, 397
MbiI CCGCTC 1 cut(s) 74
MboI GATC 2 cut(s) 53, 395
MboII GAAGA 5 cut(s) 287, 299, 475, 559, 703
MluCI AATT 5 cut(s) 207, 300, 386, 551, 681
Mly113I GGCGCC 1 cut(s) 69
MlyI GAGTC 1 cut(s) 23
MnlI CCTC 6 cut(s) 176, 201, 364, 478, 529, 678
Mph1103I ATGCAT 1 cut(s) 725
MroXI GAANNNNTTC 1 cut(s) 707
MspA1I CMGCKG 1 cut(s) 647
MvnI CGCG 2 cut(s) 230, 647
MwoI GCNNNNNNNGC 4 cut(s) 77, 154, 227, 249
NarI GGCGCC 1 cut(s) 69
NdeII GATC 2 cut(s) 53, 395
NlaIII CATG 2 cut(s) 266, 636
NlaIV GGNNCC 2 cut(s) 70, 220
NmuCI GTSAC 1 cut(s) 445
NsiI ATGCAT 1 cut(s) 725
NspI RCATGY 1 cut(s) 266
PceI AGGCCT 1 cut(s) 7
PciI ACATGT 1 cut(s) 262
PdmI GAANNNNTTC 1 cut(s) 707
PfeI GAWTC 3 cut(s) 83, 179, 289
PkrI GCNGC 7 cut(s) 73, 159, 162, 199, 202, 232, 646
PleI GAGTC 1 cut(s) 23
PluTI GGCGCC 1 cut(s) 72
PpsI GAGTC 1 cut(s) 23
PscI ACATGT 1 cut(s) 262
PspFI CCCAGC 1 cut(s) 63
PspN4I GGNNCC 2 cut(s) 70, 220
PspPI GGNCC 2 cut(s) 219, 516
PstI CTGCAG 1 cut(s) 162
RsaI GTAC 2 cut(s) 131, 281
RsaNI GTAC 2 cut(s) 130, 280
SacII CCGCGG 1 cut(s) 648
SatI GCNGC 7 cut(s) 72, 158, 161, 198, 201, 231, 645
Sau3AI GATC 2 cut(s) 53, 395
Sau96I GGNCC 2 cut(s) 219, 516
SchI GAGTC 1 cut(s) 23
SetI ASST 8 cut(s) 61, 82, 168, 193, 364, 480, 576, 709
SfaNI GCATC 2 cut(s) 403, 710
SfcI CTRYAG 1 cut(s) 158
SfoI GGCGCC 1 cut(s) 70
Sfr303I CCGCGG 1 cut(s) 648
SgrBI CCGCGG 1 cut(s) 648
SinI GGWCC 1 cut(s) 516
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
Sse9I AATT 5 cut(s) 207, 300, 386, 551, 681
SseBI AGGCCT 1 cut(s) 7
SsiI CCGC 7 cut(s) 72, 94, 126, 230, 519, 645, 647
SspDI GGCGCC 1 cut(s) 68
SspMI CTAG 2 cut(s) 77, 417
StuI AGGCCT 1 cut(s) 7
TasI AATT 5 cut(s) 207, 300, 386, 551, 681
TatI WGTACW 1 cut(s) 279
TauI GCSGC 3 cut(s) 74, 233, 647
TfiI GAWTC 3 cut(s) 83, 179, 289
TseFI GTSAC 1 cut(s) 445
TseI GCWGC 4 cut(s) 157, 160, 197, 200
Tsp45I GTSAC 1 cut(s) 445
TspDTI ATGAA 4 cut(s) 299, 399, 452, 456
VpaK11BI GGWCC 1 cut(s) 516
XapI RAATTY 2 cut(s) 386, 551
XceI RCATGY 1 cut(s) 266
XmiI GTMKAC 1 cut(s) 496
XmnI GAANNNNTTC 1 cut(s) 707
XspI CTAG 2 cut(s) 77, 417
Zsp2I ATGCAT 1 cut(s) 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.