Rroxscaffold_3G00262110

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009216
Physical Location & Seq
Forward (+)
56214929 .. 56217633
2705 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00262110.1

Sequence Viewer

Length: 912 bp
ATGAGACCTGAAATCGTGTTGTTTGGAGACTCGATAACAGAGCAGTCCTTCCAATTAGGAGGCTGGGGTGCCGCTCTTGCTGACACCTATTCTCGCAAGGCTGATGTCAAAGTTCGTGGCTATGGTGGGTACAACACCAGATGGGCGCTGTTCTTGTTGCATCAGATCTTTCCTCTTGTGCGTGTTCCTTCATTTGGTTGTGCTAATTCAATTGGGTTTTTGGTTTCTTTCTGTATTGTGTTGATTACATGTCTTATTCTGCATTTGGCATTAGAATCTCATGAAGCTCAACTTAAATTGGGTCTTGGATTAAAGAGTATTAACATAGGACTAGATGGTGTTATATTGATAGTTGAAGAGGATTCCAGAAAACCTCCTGCTGCTGCCACAATTTTCTTTGGGGCCAATGATGCAGCTATTTTGGGGAGAACTAGTGAACGGCAACATGTTCCTCTTGAAGAGTTCAAGGAGAATCTCAGAAAAATTGTTCTCCATTTGAAGGAGTGCAGTCCCACAATTCTGATTGTGCTTATCACTCCACCACCTGTGGATGAGGAAGGGCGTAATGAATTTGCACGATCTTTGTATGGTGAGGATGCTAGGAAACTGCCAGAAAGGACAAATGAAGCAGCGGAAGTTTATGCAAAAAAGTGCCTTGACCTAGCTGAGGAAATGGGTATCCGCTCCATCAACCTTTGGTCCAAGTTGCAGGAAACAGAGGGTTGGCAGAAGAAATTTCTAAGCGACGGGTTGCACCTAACACCAGAAGGCAATGCTGTAGTCCACCAAGAAGTTGTAAGAGTTTTCAAGGAAGCATGGTTTTCTGCTGACGAAATGCCTTATGATTTTCCTCACCACTCGGTAATTGATGGGAAGAACCCTGAGAAAGCTTTTGAGCAAAGATGCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

303

Amino Acids

33.94

Weight (kDa)

5.66

Isoelectric Point (pI)

43.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 5 - 264 4.9e-31 GDSL-like Lipase/Acylhydrolase
Lipase_GDSL_2 PF13472 119 - 259 2.8e-14 GDSL-like Lipase/Acylhydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 68
AccBSI CCGCTC 2 cut(s) 74, 684
AciI CCGC 3 cut(s) 72, 632, 682
AcsI RAATTY 2 cut(s) 569, 734
AfaI GTAC 1 cut(s) 131
AfiI CCNNNNNNNGG 3 cut(s) 194, 499, 667
AflIII ACRYGT 2 cut(s) 248, 445
AgsI TTSAA 6 cut(s) 210, 356, 458, 466, 499, 808
AhlI ACTAGT 1 cut(s) 431
AluBI AGCT 4 cut(s) 287, 416, 665, 890
AluI AGCT 4 cut(s) 287, 416, 665, 890
Alw26I GTCTC 1 cut(s) 21
AoxI GGCC 1 cut(s) 402
ApeKI GCWGC 4 cut(s) 380, 383, 413, 629
ApoI RAATTY 2 cut(s) 569, 734
AspLEI GCGC 1 cut(s) 148
AspS9I GGNCC 2 cut(s) 402, 699
AsuHPI GGTGA 2 cut(s) 602, 845
AvaII GGWCC 1 cut(s) 699
BanI GGYRCC 1 cut(s) 68
BbvCI CCTCAGC 1 cut(s) 666
BbvI GCAGC 4 cut(s) 367, 370, 425, 641
BccI CCATC 4 cut(s) 135, 329, 695, 863
BceAI ACGGC 1 cut(s) 455
BciVI GTATCC 1 cut(s) 689
BcoDI GTCTC 1 cut(s) 21
BcuI ACTAGT 1 cut(s) 431
BfaI CTAG 4 cut(s) 332, 432, 600, 662
BfmI CTRYAG 1 cut(s) 777
BfoI RGCGCY 1 cut(s) 149
BfuI GTATCC 1 cut(s) 689
BglII AGATCT 1 cut(s) 165
BisI GCNGC 5 cut(s) 72, 381, 384, 414, 630
BlsI GCNGC 5 cut(s) 73, 382, 385, 415, 631
Bme18I GGWCC 1 cut(s) 699
BmgT120I GGNCC 2 cut(s) 402, 699
BmiI GGNNCC 2 cut(s) 70, 403
BmsI GCATC 4 cut(s) 169, 400, 586, 893
Bpu10I CCTNAGC 1 cut(s) 666
BsaXI ACNNNNNCTCC 2 cut(s) 418, 448
Bsc4I CCNNNNNNNGG 3 cut(s) 194, 499, 667
Bse3DI GCAATG 1 cut(s) 778
BseGI GGATG 2 cut(s) 556, 601
BseLI CCNNNNNNNGG 3 cut(s) 194, 499, 667
BseMI GCAATG 1 cut(s) 778
BseMII CTCAG 3 cut(s) 490, 657, 873
BseXI GCAGC 4 cut(s) 367, 370, 425, 641
BseYI CCCAGC 1 cut(s) 63
BsgI GTGCAG 1 cut(s) 526
BshFI GGCC 1 cut(s) 404
BshNI GGYRCC 1 cut(s) 68
BslFI GGGAC 1 cut(s) 495
BslI CCNNNNNNNGG 3 cut(s) 194, 499, 667
BsmAI GTCTC 1 cut(s) 21
BsmFI GGGAC 1 cut(s) 495
BsnI GGCC 1 cut(s) 404
Bsp143I GATC 2 cut(s) 165, 578
BspACI CCGC 3 cut(s) 72, 632, 682
BspANI GGCC 1 cut(s) 404
BspCNI CTCAG 3 cut(s) 489, 658, 874
BspHI TCATGA 1 cut(s) 280
BspLI GGNNCC 2 cut(s) 70, 403
BspT107I GGYRCC 1 cut(s) 68
BsrBI CCGCTC 2 cut(s) 74, 684
BsrDI GCAATG 1 cut(s) 778
BssMI GATC 2 cut(s) 165, 578
Bst6I CTCTTC 2 cut(s) 351, 453
BstDEI CTNAG 4 cut(s) 476, 666, 740, 882
BstENI CCTNNNNNAGG 1 cut(s) 665
BstF5I GGATG 2 cut(s) 556, 601
BstH2I RGCGCY 1 cut(s) 149
BstHHI GCGC 1 cut(s) 148
BstKTI GATC 2 cut(s) 168, 581
BstMAI GTCTC 1 cut(s) 21
BstMBI GATC 2 cut(s) 165, 578
BstMWI GCNNNNNNNGC 2 cut(s) 77, 410
BstNSI RCATGY 2 cut(s) 252, 449
BstSFI CTRYAG 1 cut(s) 777
BstV1I GCAGC 4 cut(s) 367, 370, 425, 641
BstX2I RGATCY 1 cut(s) 165
BstYI RGATCY 1 cut(s) 165
BsuI GTATCC 1 cut(s) 689
BsuRI GGCC 1 cut(s) 404
BtsCI GGATG 2 cut(s) 556, 601
CciI TCATGA 1 cut(s) 280
CfoI GCGC 1 cut(s) 148
Cfr13I GGNCC 2 cut(s) 402, 699
Csp6I GTAC 1 cut(s) 130
CspCI CAANNNNNGTGG 2 cut(s) 97, 132
CviAII CATG 4 cut(s) 249, 281, 446, 816
CviJI RGCY 8 cut(s) 63, 101, 120, 287, 404, 416, 665, 890
CviKI_1 RGCY 8 cut(s) 63, 101, 120, 287, 404, 416, 665, 890
CviQI GTAC 1 cut(s) 130
DdeI CTNAG 4 cut(s) 476, 666, 740, 882
DpnI GATC 2 cut(s) 167, 580
DpnII GATC 2 cut(s) 165, 578
Eam1104I CTCTTC 2 cut(s) 351, 453
EarI CTCTTC 2 cut(s) 351, 453
Eco47I GGWCC 1 cut(s) 699
EcoNI CCTNNNNNAGG 1 cut(s) 665
EcoT22I ATGCAT 1 cut(s) 908
FaeI CATG 4 cut(s) 252, 284, 449, 819
FalI AAGNNNNNCTT 2 cut(s) 276, 308
FaqI GGGAC 1 cut(s) 495
FatI CATG 4 cut(s) 248, 280, 445, 815
Fnu4HI GCNGC 5 cut(s) 72, 381, 384, 414, 630
FokI GGATG 2 cut(s) 563, 608
Fsp4HI GCNGC 5 cut(s) 72, 381, 384, 414, 630
FspBI CTAG 4 cut(s) 332, 432, 600, 662
GlaI GCGC 1 cut(s) 147
GluI GCNGC 5 cut(s) 72, 381, 384, 414, 630
GsaI CCCAGC 1 cut(s) 67
HaeII RGCGCY 1 cut(s) 149
HaeIII GGCC 1 cut(s) 404
HhaI GCGC 1 cut(s) 148
Hin1II CATG 4 cut(s) 252, 284, 449, 819
Hin6I GCGC 1 cut(s) 146
HinP1I GCGC 1 cut(s) 146
HindIII AAGCTT 1 cut(s) 888
HinfI GANTC 4 cut(s) 29, 275, 362, 472
HphI GGTGA 2 cut(s) 602, 845
Hpy166II GTNNAC 2 cut(s) 437, 784
Hpy188I TCNGA 3 cut(s) 165, 479, 522
Hpy188III TCNNGA 3 cut(s) 281, 366, 455
Hpy8I GTNNAC 2 cut(s) 437, 784
Hpy99I CGWCG 1 cut(s) 749
HpyAV CCTTC 5 cut(s) 58, 198, 493, 551, 761
HpyCH4V TGCA 9 cut(s) 160, 262, 413, 507, 575, 644, 709, 754, 906
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 410
HpyF3I CTNAG 4 cut(s) 476, 666, 740, 882
Hsp92II CATG 4 cut(s) 252, 284, 449, 819
HspAI GCGC 1 cut(s) 146
Kzo9I GATC 2 cut(s) 165, 578
LmnI GCTCC 1 cut(s) 689
Lsp1109I GCAGC 4 cut(s) 367, 370, 425, 641
LweI GCATC 4 cut(s) 169, 400, 586, 893
MaeI CTAG 4 cut(s) 332, 432, 600, 662
MalI GATC 2 cut(s) 167, 580
MbiI CCGCTC 2 cut(s) 74, 684
MboI GATC 2 cut(s) 165, 578
MboII GAAGA 4 cut(s) 368, 470, 742, 886
MfeI CAATTG 1 cut(s) 210
MflI RGATCY 1 cut(s) 165
MlyI GAGTC 1 cut(s) 23
Mph1103I ATGCAT 1 cut(s) 908
MseI TTAA 3 cut(s) 294, 311, 321
MspA1I CMGCKG 1 cut(s) 632
MunI CAATTG 1 cut(s) 210
MwoI GCNNNNNNNGC 2 cut(s) 77, 410
NdeII GATC 2 cut(s) 165, 578
NlaIII CATG 4 cut(s) 252, 284, 449, 819
NlaIV GGNNCC 2 cut(s) 70, 403
NsiI ATGCAT 1 cut(s) 908
NspI RCATGY 2 cut(s) 252, 449
PagI TCATGA 1 cut(s) 280
PciI ACATGT 2 cut(s) 248, 445
PfeI GAWTC 3 cut(s) 275, 362, 472
PkrI GCNGC 5 cut(s) 73, 382, 385, 415, 631
PleI GAGTC 1 cut(s) 23
PpsI GAGTC 1 cut(s) 23
PscI ACATGT 2 cut(s) 248, 445
PspFI CCCAGC 1 cut(s) 63
PspN4I GGNNCC 2 cut(s) 70, 403
PspPI GGNCC 2 cut(s) 402, 699
PsuI RGATCY 1 cut(s) 165
RsaI GTAC 1 cut(s) 131
RsaNI GTAC 1 cut(s) 130
SaqAI TTAA 3 cut(s) 294, 311, 321
SatI GCNGC 5 cut(s) 72, 381, 384, 414, 630
Sau3AI GATC 2 cut(s) 165, 578
Sau96I GGNCC 2 cut(s) 402, 699
SchI GAGTC 1 cut(s) 23
SfaNI GCATC 4 cut(s) 169, 400, 586, 893
SfcI CTRYAG 1 cut(s) 777
SinI GGWCC 1 cut(s) 699
SpeI ACTAGT 1 cut(s) 431
SsiI CCGC 3 cut(s) 72, 632, 682
SspMI CTAG 4 cut(s) 332, 432, 600, 662
TaqI TCGA 1 cut(s) 32
TauI GCSGC 1 cut(s) 74
TfiI GAWTC 3 cut(s) 275, 362, 472
Tru1I TTAA 3 cut(s) 294, 311, 321
Tru9I TTAA 3 cut(s) 294, 311, 321
TseI GCWGC 4 cut(s) 380, 383, 413, 629
TspDTI ATGAA 4 cut(s) 180, 297, 582, 639
VpaK11BI GGWCC 1 cut(s) 699
XagI CCTNNNNNAGG 1 cut(s) 665
XapI RAATTY 2 cut(s) 569, 734
XceI RCATGY 2 cut(s) 252, 449
XspI CTAG 4 cut(s) 332, 432, 600, 662
Zsp2I ATGCAT 1 cut(s) 908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.