Rorug07G0009400

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0009216
Physical Location & Seq
Forward (+)
776966 .. 778243
1278 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0009400.1

Sequence Viewer

Length: 966 bp
ATGGCTCTCATCCGGGAACGCCGCCAGCTCAACCTCTGCAGCCTCCCCTTGCCGGAACCCTCCGAGCGTCGCCCACGTTTCACTCTACCTCTCCCTCCCACCGCCGCCGTGGCAACCACCACCAACTGCTCCTCCGCCGCCGCCGCCTCCGACCTCGAAAGACTAGAGGTACTAGGCCACGGTAACGGCGGCACAGTCTACAAAGTCCGCCACAAGCGCACTGGAGCTAACTACGCCCTAAAAATAGTGAAAGGCGACTCCGACCCCACCGTCCGCCGCCAGCTCTACCGCGAAATAGAAATCCTCCGCCGCACGGACTCCCCCCACGTGGTTCACTGCAACGCCATAATCGAGAAGCCCACCGGAGACATCTGGATTCTCATGGAGTACATGGACTCGGGAACATTAGAGACCTCGCTCAAAGAAAACGGCACGTTCTCCGAGGCCAAGGTCGCTCATGTAGCTCGTCAAGTCCTCAACGGTCTCAATTACCTCCACACTCAGAAGATCATCCACCGTGACATCAAGCCAGCCAATCTGTTAGTCAACAGCAACATGGAAGTCAAGATTGCCGACTTCGGCGTCAGCAAAATGATGTGCCGAACTCTCGATAACTGTAACTCATACGTCGGGACTTGTGCTTACATGAGCCCGGAGAGATTTGACCCTGACACTTACGGTGGAAACTACAATGGATACGCCAGTGATATATGGAGCTTGGGTCTGACCCTCATGGAGCTTTACATGGGCCACTTTCCGTTCTTGCCTCCGGGTCAGAGACCCGACTGGGCGACCCTTATGTGCGCCATATGTTTCGGGGAGCCACCGGCCTTGCCGGAGGGGGTGTCGGAGGAGTTTAGGAGTTTTATGGAGTGTTGCTTGCAAAAGGAGTCGGGGAAGAGGTGGACGGCGGCTCAGCTTTTGACGCACCCGTTTGTTTGTAAAGATCCGAAAGCCATTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

321

Amino Acids

35.95

Weight (kDa)

7.97

Isoelectric Point (pI)

51.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 53 - 313 3.7e-67 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 53 - 308 3e-41 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 115 - 199 3.8e-06 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 269, 581
AccI GTMKAC 1 cut(s) 198
AccII CGCG 1 cut(s) 291
AclWI GGATC 1 cut(s) 941
AcvI CACGTG 1 cut(s) 328
AcyI GRCGYC 1 cut(s) 582
AfaI GTAC 2 cut(s) 171, 389
AfiI CCNNNNNNNGG 3 cut(s) 52, 313, 328
AluBI AGCT 7 cut(s) 28, 227, 283, 464, 717, 739, 919
AluI AGCT 7 cut(s) 28, 227, 283, 464, 717, 739, 919
Alw26I GTCTC 4 cut(s) 360, 404, 488, 772
AlwI GGATC 1 cut(s) 941
Ama87I CYCGRG 1 cut(s) 397
AoxI GGCC 4 cut(s) 175, 444, 748, 828
ApeKI GCWGC 1 cut(s) 39
AspLEI GCGC 2 cut(s) 219, 806
AspS9I GGNCC 1 cut(s) 748
AsuC2I CCSGG 3 cut(s) 14, 653, 771
AvaI CYCGRG 1 cut(s) 397
BaeI ACNNNNGTAYC 2 cut(s) 688, 721
BanII GRGCYC 1 cut(s) 653
BbrPI CACGTG 1 cut(s) 328
BbvI GCAGC 1 cut(s) 51
BceAI ACGGC 4 cut(s) 92, 202, 445, 924
BciVI GTATCC 1 cut(s) 689
BcnI CCSGG 3 cut(s) 14, 653, 771
BcoDI GTCTC 4 cut(s) 360, 404, 488, 772
BfaI CTAG 2 cut(s) 164, 173
BfmI CTRYAG 1 cut(s) 37
BfuI GTATCC 1 cut(s) 689
BglI GCCNNNNNGGC 1 cut(s) 110
BlpI GCTNAGC 1 cut(s) 915
Bme1390I CCNGG 3 cut(s) 14, 653, 771
BmeT110I CYCGRG 1 cut(s) 397
BmgT120I GGNCC 1 cut(s) 748
BmiI GGNNCC 2 cut(s) 57, 822
BmrFI CCNGG 3 cut(s) 14, 653, 771
BmrI ACTGGG 1 cut(s) 796
BmuI ACTGGG 1 cut(s) 796
BpmI CTGGAG 1 cut(s) 243
Bpu1102I GCTNAGC 1 cut(s) 915
BpuMI CCSGG 3 cut(s) 14, 653, 771
BsaAI YACGTR 1 cut(s) 328
BsaHI GRCGYC 1 cut(s) 582
BsaI GGTCTC 3 cut(s) 404, 488, 772
BsaJI CCNNGG 4 cut(s) 108, 178, 441, 447
BsaWI WCCGGW 1 cut(s) 362
BsaXI ACNNNNNCTCC 6 cut(s) 116, 146, 706, 736, 830, 860
Bsc4I CCNNNNNNNGG 3 cut(s) 52, 313, 328
Bse118I RCCGGY 1 cut(s) 826
Bse1I ACTGG 3 cut(s) 226, 702, 791
BseDI CCNNGG 4 cut(s) 108, 178, 441, 447
BseGI GGATG 2 cut(s) 9, 510
BseLI CCNNNNNNNGG 3 cut(s) 52, 313, 328
BseMII CTCAG 2 cut(s) 515, 929
BseNI ACTGG 3 cut(s) 226, 702, 791
BseRI GAGGAG 2 cut(s) 121, 866
BseXI GCAGC 1 cut(s) 51
Bsh1236I CGCG 1 cut(s) 291
BshFI GGCC 4 cut(s) 177, 446, 750, 830
BsiHKCI CYCGRG 1 cut(s) 397
BsiSI CCGG 7 cut(s) 13, 53, 363, 653, 770, 827, 836
BslFI GGGAC 1 cut(s) 646
BslI CCNNNNNNNGG 3 cut(s) 52, 313, 328
BsmAI GTCTC 4 cut(s) 360, 404, 488, 772
BsmFI GGGAC 1 cut(s) 646
BsnI GGCC 4 cut(s) 177, 446, 750, 830
Bso31I GGTCTC 3 cut(s) 404, 488, 772
BsoBI CYCGRG 1 cut(s) 397
Bsp1286I GDGCHC 1 cut(s) 653
Bsp143I GATC 2 cut(s) 507, 946
Bsp1720I GCTNAGC 1 cut(s) 915
BspANI GGCC 4 cut(s) 177, 446, 750, 830
BspCNI CTCAG 2 cut(s) 514, 928
BspFNI CGCG 1 cut(s) 291
BspLI GGNNCC 2 cut(s) 57, 822
BspMAI CTGCAG 1 cut(s) 41
BspPI GGATC 1 cut(s) 941
BspTNI GGTCTC 3 cut(s) 404, 488, 772
BsrFI RCCGGY 1 cut(s) 826
BsrI ACTGG 3 cut(s) 226, 702, 791
BssAI RCCGGY 1 cut(s) 826
BssECI CCNNGG 4 cut(s) 108, 178, 441, 447
BssMI GATC 2 cut(s) 507, 946
BssNI GRCGYC 1 cut(s) 582
BssT1I CCWWGG 1 cut(s) 447
Bst4CI ACNGT 7 cut(s) 182, 196, 271, 482, 518, 617, 680
Bst6I CTCTTC 1 cut(s) 893
BstACI GRCGYC 1 cut(s) 582
BstBAI YACGTR 1 cut(s) 328
BstC8I GCNNGC 4 cut(s) 26, 281, 531, 881
BstDEI CTNAG 2 cut(s) 501, 915
BstDSI CCRYGG 2 cut(s) 108, 178
BstF5I GGATG 2 cut(s) 9, 510
BstFNI CGCG 1 cut(s) 291
BstHHI GCGC 2 cut(s) 219, 806
BstKTI GATC 2 cut(s) 510, 949
BstMAI GTCTC 4 cut(s) 360, 404, 488, 772
BstMBI GATC 2 cut(s) 507, 946
BstMWI GCNNNNNNNGC 7 cut(s) 110, 143, 216, 233, 452, 461, 925
BstSCI CCNGG 3 cut(s) 12, 651, 769
BstSFI CTRYAG 1 cut(s) 37
BstUI CGCG 1 cut(s) 291
BstV1I GCAGC 1 cut(s) 51
BstX2I RGATCY 1 cut(s) 946
BstYI RGATCY 1 cut(s) 946
BsuI GTATCC 1 cut(s) 689
BsuRI GGCC 4 cut(s) 177, 446, 750, 830
BtgI CCRYGG 2 cut(s) 108, 178
BtsCI GGATG 2 cut(s) 9, 510
BtsI GCAGTG 1 cut(s) 334
BtsIMutI CAGTG 3 cut(s) 219, 334, 709
Cac8I GCNNGC 4 cut(s) 26, 281, 531, 881
CfoI GCGC 2 cut(s) 219, 806
Cfr10I RCCGGY 1 cut(s) 826
Cfr13I GGNCC 1 cut(s) 748
CseI GACGC 3 cut(s) 56, 571, 934
Csp6I GTAC 2 cut(s) 170, 388
CspCI CAANNNNNGTGG 2 cut(s) 813, 848
CviAII CATG 7 cut(s) 382, 391, 458, 556, 646, 733, 745
CviQI GTAC 2 cut(s) 170, 388
DdeI CTNAG 2 cut(s) 501, 915
DpnI GATC 2 cut(s) 509, 948
DpnII GATC 2 cut(s) 507, 946
DrdI GACNNNNNNGTC 2 cut(s) 269, 581
DseDI GACNNNNNNGTC 2 cut(s) 269, 581
Eam1104I CTCTTC 1 cut(s) 893
EarI CTCTTC 1 cut(s) 893
EciI GGCGGA 4 cut(s) 124, 197, 263, 296
Eco130I CCWWGG 1 cut(s) 447
Eco24I GRGCYC 1 cut(s) 653
Eco31I GGTCTC 3 cut(s) 404, 488, 772
Eco72I CACGTG 1 cut(s) 328
Eco88I CYCGRG 1 cut(s) 397
EcoT14I CCWWGG 1 cut(s) 447
EcoT38I GRGCYC 1 cut(s) 653
ErhI CCWWGG 1 cut(s) 447
FaeI CATG 7 cut(s) 385, 394, 461, 559, 649, 736, 748
FaqI GGGAC 1 cut(s) 646
FatI CATG 7 cut(s) 381, 390, 457, 555, 645, 732, 744
FauNDI CATATG 1 cut(s) 809
FblI GTMKAC 1 cut(s) 198
FokI GGATG 1 cut(s) 497
FriOI GRGCYC 1 cut(s) 653
FspBI CTAG 2 cut(s) 164, 173
GlaI GCGC 2 cut(s) 218, 805
GsuI CTGGAG 1 cut(s) 243
HaeIII GGCC 4 cut(s) 177, 446, 750, 830
HapII CCGG 7 cut(s) 13, 53, 363, 653, 770, 827, 836
HgaI GACGC 3 cut(s) 56, 571, 934
HhaI GCGC 2 cut(s) 219, 806
Hin1I GRCGYC 1 cut(s) 582
Hin1II CATG 7 cut(s) 385, 394, 461, 559, 649, 736, 748
Hin6I GCGC 2 cut(s) 217, 804
HinP1I GCGC 2 cut(s) 217, 804
HincII GTYRAC 1 cut(s) 547
HindII GTYRAC 1 cut(s) 547
HinfI GANTC 5 cut(s) 257, 317, 376, 395, 890
HpaII CCGG 7 cut(s) 13, 53, 363, 653, 770, 827, 836
Hpy166II GTNNAC 4 cut(s) 199, 334, 547, 906
Hpy188I TCNGA 9 cut(s) 64, 151, 262, 442, 504, 726, 777, 850, 951
Hpy188III TCNNGA 6 cut(s) 352, 373, 399, 565, 608, 631
Hpy8I GTNNAC 4 cut(s) 199, 334, 547, 906
Hpy99I CGWCG 2 cut(s) 72, 632
HpyCH4III ACNGT 7 cut(s) 182, 196, 271, 482, 518, 617, 680
HpyCH4IV ACGT 4 cut(s) 76, 327, 434, 627
HpyCH4V TGCA 3 cut(s) 39, 339, 883
HpyF10VI GCNNNNNNNGC 7 cut(s) 110, 143, 216, 233, 452, 461, 925
HpyF3I CTNAG 2 cut(s) 501, 915
HpySE526I ACGT 4 cut(s) 76, 327, 434, 627
Hsp92I GRCGYC 1 cut(s) 582
Hsp92II CATG 7 cut(s) 385, 394, 461, 559, 649, 736, 748
HspAI GCGC 2 cut(s) 217, 804
Kzo9I GATC 2 cut(s) 507, 946
LmnI GCTCC 5 cut(s) 134, 224, 714, 736, 820
Lsp1109I GCAGC 1 cut(s) 51
MaeI CTAG 2 cut(s) 164, 173
MaeII ACGT 4 cut(s) 76, 327, 434, 627
MaeIII GTNAC 3 cut(s) 182, 518, 617
MalI GATC 2 cut(s) 509, 948
MboI GATC 2 cut(s) 507, 946
MboII GAAGA 2 cut(s) 517, 910
MflI RGATCY 1 cut(s) 946
MhlI GDGCHC 1 cut(s) 653
MluCI AATT 1 cut(s) 487
MlyI GAGTC 4 cut(s) 251, 311, 389, 899
MmeI TCCRAC 3 cut(s) 174, 285, 828
MspI CCGG 7 cut(s) 13, 53, 363, 653, 770, 827, 836
MspR9I CCNGG 3 cut(s) 14, 653, 771
MvnI CGCG 1 cut(s) 291
MwoI GCNNNNNNNGC 7 cut(s) 110, 143, 216, 233, 452, 461, 925
NciI CCSGG 3 cut(s) 14, 653, 771
NdeI CATATG 1 cut(s) 809
NdeII GATC 2 cut(s) 507, 946
NlaIII CATG 7 cut(s) 385, 394, 461, 559, 649, 736, 748
NlaIV GGNNCC 2 cut(s) 57, 822
NmuCI GTSAC 1 cut(s) 518
PcsI WCGNNNNNNNCGW 1 cut(s) 348
PfeI GAWTC 1 cut(s) 376
PfoI TCCNGGA 1 cut(s) 12
PleI GAGTC 4 cut(s) 251, 311, 389, 898
PmaCI CACGTG 1 cut(s) 328
PmlI CACGTG 1 cut(s) 328
PpsI GAGTC 4 cut(s) 251, 311, 389, 898
Ppu21I YACGTR 1 cut(s) 328
PspCI CACGTG 1 cut(s) 328
PspN4I GGNNCC 2 cut(s) 57, 822
PspPI GGNCC 1 cut(s) 748
PstI CTGCAG 1 cut(s) 41
PsuI RGATCY 1 cut(s) 946
RsaI GTAC 2 cut(s) 171, 389
RsaNI GTAC 2 cut(s) 170, 388
Sau3AI GATC 2 cut(s) 507, 946
Sau96I GGNCC 1 cut(s) 748
SchI GAGTC 4 cut(s) 251, 311, 389, 899
ScrFI CCNGG 3 cut(s) 14, 653, 771
SduI GDGCHC 1 cut(s) 653
SfcI CTRYAG 1 cut(s) 37
Sse9I AATT 1 cut(s) 487
SspMI CTAG 2 cut(s) 164, 173
StyD4I CCNGG 3 cut(s) 12, 651, 769
StyI CCWWGG 1 cut(s) 447
TaaI ACNGT 7 cut(s) 182, 196, 271, 482, 518, 617, 680
TaiI ACGT 4 cut(s) 79, 330, 437, 630
TaqI TCGA 3 cut(s) 156, 351, 609
TasI AATT 1 cut(s) 487
TatI WGTACW 1 cut(s) 387
TauI GCSGC 9 cut(s) 24, 107, 140, 143, 146, 192, 279, 312, 914
TfiI GAWTC 1 cut(s) 376
TscAI CASTG 3 cut(s) 226, 341, 709
TseFI GTSAC 1 cut(s) 518
TseI GCWGC 1 cut(s) 39
Tsp45I GTSAC 1 cut(s) 518
TspGWI ACGGA 2 cut(s) 329, 747
TspRI CASTG 3 cut(s) 226, 341, 709
XcmI CCANNNNNNNNNTGG 2 cut(s) 106, 218
XmiI GTMKAC 1 cut(s) 198
XspI CTAG 2 cut(s) 164, 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.