MD14G1179100.v1.1

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009216
Physical Location & Seq
Reverse (-)
27171095 .. 27173640
2546 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1179100.v1.1.491

Sequence Viewer

Length: 732 bp
ATGATGAGGCCCGAGATTGTATTGTTCGGAGACTCAATCACCCAACAATCCTTCCGACCAGGTGGCTGGGGCGCCGCTCTAGCTGATTCTTACTCCCGCAAGGCCGATGTAAAAGTTCGTGGGTATGGCGGGTACAACACGAGATGGGCACTGTTCTTGCTGCAGCACCTCTTTCCTTTGGATTCTAAGAAACCTCCTGCTGCTGCCACAATTTTCTTTGGGGCCAATGACGCAGCTGTTTTAGGCAGAACAGGTGAACGGCAACATGTTCCCGTTGAAGAGTACAAGGAGAATCTCAGAAAAATTGTTCTTCATCTCAAGGAGTGTTCTCCCACCATTTTGATTGTGCTTATTACTCCACCACCTGTTGACGAGGATGGCCGTAATGAATTTGCGCAATCTTTATATGGTAAGGATGCTAGTGGACTTCCAGAAAGGACGAATGAAGTGACAGGAGTTTATGCGAAGAAGTGCATTGAGCTAGCTGAGGAAATGGGTCTACGCTCCATCAATCTTTGGTCCGCAATGCAGGAAACGGAGGGTTGGCAGAAGAAATTTCTAAGTGACGGGTTGCACCTAACACCAGAAGGCAATGCTGTTGTCCACCAAGAAGTCGTCAAAGTTTTCAGTGAAGCATGGCTTTCTGCCGCGGAAATGCCTTATGATTTCCCTCACCACTCAGAAATTGATGGGAAGAACCCTGAGAAAGCTTTCCAGCAACGATGCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

244

Amino Acids

27.19

Weight (kDa)

5.84

Isoelectric Point (pI)

48.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 6 - 204 3.3e-33 GDSL-like Lipase/Acylhydrolase
Lipase_GDSL_2 PF13472 8 - 199 3.4e-25 GDSL-like Lipase/Acylhydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 396
AccB1I GGYRCC 1 cut(s) 71
AccBSI CCGCTC 1 cut(s) 77
AccI GTMKAC 1 cut(s) 499
AccII CGCG 1 cut(s) 650
AciI CCGC 6 cut(s) 75, 97, 129, 522, 648, 650
AcoI YGGCCR 1 cut(s) 379
AcsI RAATTY 2 cut(s) 389, 554
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 2 cut(s) 134, 284
AflIII ACRYGT 1 cut(s) 265
AgsI TTSAA 1 cut(s) 278
AjnI CCWGG 1 cut(s) 58
AluBI AGCT 5 cut(s) 83, 236, 481, 485, 710
AluI AGCT 5 cut(s) 83, 236, 481, 485, 710
Alw26I GTCTC 1 cut(s) 24
Ama87I CYCGRG 1 cut(s) 11
AoxI GGCC 4 cut(s) 8, 102, 222, 379
ApeKI GCWGC 5 cut(s) 160, 163, 200, 203, 233
ApoI RAATTY 2 cut(s) 389, 554
Asp700I GAANNNNTTC 1 cut(s) 710
AspLEI GCGC 2 cut(s) 74, 397
AspS9I GGNCC 3 cut(s) 9, 222, 519
AsuHPI GGTGA 3 cut(s) 31, 266, 665
AsuNHI GCTAGC 1 cut(s) 481
AvaI CYCGRG 1 cut(s) 11
AvaII GGWCC 1 cut(s) 519
BaeGI GKGCMC 1 cut(s) 151
BanI GGYRCC 1 cut(s) 71
BauI CACGAG 1 cut(s) 139
BbvCI CCTCAGC 1 cut(s) 486
BbvI GCAGC 5 cut(s) 147, 175, 187, 190, 245
BccI CCATC 4 cut(s) 138, 371, 515, 683
BceAI ACGGC 2 cut(s) 275, 366
BciT130I CCWGG 1 cut(s) 60
BcoDI GTCTC 1 cut(s) 24
BfaI CTAG 3 cut(s) 80, 420, 482
BfmI CTRYAG 1 cut(s) 161
BfoI RGCGCY 1 cut(s) 75
BisI GCNGC 7 cut(s) 75, 161, 164, 201, 204, 234, 648
BlsI GCNGC 7 cut(s) 76, 162, 165, 202, 205, 235, 649
Bme1390I CCNGG 1 cut(s) 60
Bme18I GGWCC 1 cut(s) 519
BmeT110I CYCGRG 1 cut(s) 11
BmgT120I GGNCC 3 cut(s) 9, 222, 519
BmiI GGNNCC 2 cut(s) 73, 223
BmrFI CCNGG 1 cut(s) 60
BmsI GCATC 2 cut(s) 406, 713
BmtI GCTAGC 1 cut(s) 485
Bpu10I CCTNAGC 1 cut(s) 486
BpuEI CTTGAG 1 cut(s) 302
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 1 cut(s) 648
Bse3DI GCAATG 2 cut(s) 531, 598
BseBI CCWGG 1 cut(s) 60
BseDI CCNNGG 1 cut(s) 648
BseGI GGATG 2 cut(s) 382, 421
BseMI GCAATG 2 cut(s) 531, 598
BseMII CTCAG 4 cut(s) 310, 477, 693, 693
BseSI GKGCMC 1 cut(s) 151
BseXI GCAGC 5 cut(s) 147, 175, 187, 190, 245
BseYI CCCAGC 1 cut(s) 66
Bsh1236I CGCG 1 cut(s) 650
BshFI GGCC 4 cut(s) 10, 104, 224, 381
BshNI GGYRCC 1 cut(s) 71
BsiHKCI CYCGRG 1 cut(s) 11
BsmAI GTCTC 1 cut(s) 24
BsnI GGCC 4 cut(s) 10, 104, 224, 381
BsoBI CYCGRG 1 cut(s) 11
Bsp1286I GDGCHC 1 cut(s) 151
BspACI CCGC 6 cut(s) 75, 97, 129, 522, 648, 650
BspANI GGCC 4 cut(s) 10, 104, 224, 381
BspCNI CTCAG 4 cut(s) 309, 478, 692, 694
BspFNI CGCG 1 cut(s) 650
BspLI GGNNCC 2 cut(s) 73, 223
BspMAI CTGCAG 1 cut(s) 165
BspOI GCTAGC 1 cut(s) 485
BspT107I GGYRCC 1 cut(s) 71
BsrBI CCGCTC 1 cut(s) 77
BsrDI GCAATG 2 cut(s) 531, 598
BssECI CCNNGG 1 cut(s) 648
BssNI GRCGYC 1 cut(s) 72
BssSI CACGAG 1 cut(s) 139
Bst2BI CACGAG 1 cut(s) 139
Bst2UI CCWGG 1 cut(s) 60
Bst4CI ACNGT 1 cut(s) 153
Bst6I CTCTTC 1 cut(s) 273
BstACI GRCGYC 1 cut(s) 72
BstC8I GCNNGC 1 cut(s) 483
BstDEI CTNAG 6 cut(s) 186, 296, 486, 560, 679, 702
BstDSI CCRYGG 1 cut(s) 648
BstF5I GGATG 2 cut(s) 382, 421
BstFNI CGCG 1 cut(s) 650
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 2 cut(s) 74, 397
BstMAI GTCTC 1 cut(s) 24
BstMWI GCNNNNNNNGC 2 cut(s) 80, 230
BstNI CCWGG 1 cut(s) 60
BstNSI RCATGY 1 cut(s) 269
BstSCI CCNGG 1 cut(s) 58
BstSFI CTRYAG 1 cut(s) 161
BstSLI GKGCMC 1 cut(s) 151
BstUI CGCG 1 cut(s) 650
BstV1I GCAGC 5 cut(s) 147, 175, 187, 190, 245
BstXI CCANNNNNNTGG 1 cut(s) 66
BsuRI GGCC 4 cut(s) 10, 104, 224, 381
BtgI CCRYGG 1 cut(s) 648
BtsCI GGATG 2 cut(s) 382, 421
BtsIMutI CAGTG 2 cut(s) 149, 634
Cac8I GCNNGC 1 cut(s) 483
CfoI GCGC 2 cut(s) 74, 397
Cfr13I GGNCC 3 cut(s) 9, 222, 519
Cfr42I CCGCGG 1 cut(s) 651
CseI GACGC 1 cut(s) 239
CsiI ACCWGGT 1 cut(s) 58
Csp6I GTAC 2 cut(s) 133, 283
CviAII CATG 2 cut(s) 266, 636
CviQI GTAC 2 cut(s) 133, 283
DdeI CTNAG 6 cut(s) 186, 296, 486, 560, 679, 702
DinI GGCGCC 1 cut(s) 73
EaeI YGGCCR 1 cut(s) 379
Eam1104I CTCTTC 1 cut(s) 273
EarI CTCTTC 1 cut(s) 273
Eco47I GGWCC 1 cut(s) 519
Eco88I CYCGRG 1 cut(s) 11
EcoRII CCWGG 1 cut(s) 58
EcoT22I ATGCAT 1 cut(s) 728
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
FaeI CATG 2 cut(s) 269, 639
FaiI YATR 9 cut(s) 126, 267, 406, 408, 462, 637, 663, 728, 730
FalI AAGNNNNNCTT 2 cut(s) 624, 656
FatI CATG 2 cut(s) 265, 635
FauI CCCGC 2 cut(s) 104, 122
FblI GTMKAC 1 cut(s) 499
Fnu4HI GCNGC 7 cut(s) 75, 161, 164, 201, 204, 234, 648
FokI GGATG 2 cut(s) 389, 428
Fsp4HI GCNGC 7 cut(s) 75, 161, 164, 201, 204, 234, 648
FspBI CTAG 3 cut(s) 80, 420, 482
FspI TGCGCA 1 cut(s) 396
GlaI GCGC 2 cut(s) 73, 396
GluI GCNGC 7 cut(s) 75, 161, 164, 201, 204, 234, 648
GsaI CCCAGC 1 cut(s) 70
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 4 cut(s) 10, 104, 224, 381
HgaI GACGC 1 cut(s) 239
HhaI GCGC 2 cut(s) 74, 397
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 2 cut(s) 269, 639
Hin6I GCGC 2 cut(s) 72, 395
HinP1I GCGC 2 cut(s) 72, 395
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HindIII AAGCTT 1 cut(s) 708
HinfI GANTC 4 cut(s) 32, 86, 182, 292
HphI GGTGA 3 cut(s) 31, 266, 665
Hpy166II GTNNAC 5 cut(s) 257, 370, 425, 500, 604
Hpy188I TCNGA 4 cut(s) 29, 56, 299, 682
Hpy188III TCNNGA 1 cut(s) 431
Hpy8I GTNNAC 5 cut(s) 257, 370, 425, 500, 604
HpyAV CCTTC 2 cut(s) 61, 581
HpyCH4III ACNGT 1 cut(s) 153
HpyCH4V TGCA 5 cut(s) 163, 474, 529, 574, 726
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 230
HpyF3I CTNAG 6 cut(s) 186, 296, 486, 560, 679, 702
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 2 cut(s) 269, 639
HspAI GCGC 2 cut(s) 72, 395
KasI GGCGCC 1 cut(s) 71
KspI CCGCGG 1 cut(s) 651
LmnI GCTCC 1 cut(s) 509
Lsp1109I GCAGC 5 cut(s) 147, 175, 187, 190, 245
LweI GCATC 2 cut(s) 406, 713
MabI ACCWGGT 1 cut(s) 58
MaeI CTAG 3 cut(s) 80, 420, 482
MaeIII GTNAC 2 cut(s) 448, 563
MbiI CCGCTC 1 cut(s) 77
MboII GAAGA 5 cut(s) 290, 302, 478, 562, 706
MhlI GDGCHC 1 cut(s) 151
MluCI AATT 5 cut(s) 210, 303, 389, 554, 684
Mly113I GGCGCC 1 cut(s) 72
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 1 cut(s) 79
MnlI CCTC 6 cut(s) 179, 204, 367, 481, 532, 681
Mph1103I ATGCAT 1 cut(s) 728
MroXI GAANNNNTTC 1 cut(s) 710
MspA1I CMGCKG 2 cut(s) 236, 650
MspR9I CCNGG 1 cut(s) 60
MvaI CCWGG 1 cut(s) 60
MvnI CGCG 1 cut(s) 650
MwoI GCNNNNNNNGC 2 cut(s) 80, 230
NarI GGCGCC 1 cut(s) 72
NheI GCTAGC 1 cut(s) 481
NlaIII CATG 2 cut(s) 269, 639
NlaIV GGNNCC 2 cut(s) 73, 223
NmuCI GTSAC 2 cut(s) 448, 563
NsbI TGCGCA 1 cut(s) 396
NsiI ATGCAT 1 cut(s) 728
NspI RCATGY 1 cut(s) 269
PciI ACATGT 1 cut(s) 265
PdmI GAANNNNTTC 1 cut(s) 710
PfeI GAWTC 3 cut(s) 86, 182, 292
PkrI GCNGC 7 cut(s) 76, 162, 165, 202, 205, 235, 649
PleI GAGTC 1 cut(s) 26
PluTI GGCGCC 1 cut(s) 75
PpsI GAGTC 1 cut(s) 26
PscI ACATGT 1 cut(s) 265
Psp6I CCWGG 1 cut(s) 58
PspFI CCCAGC 1 cut(s) 66
PspGI CCWGG 1 cut(s) 58
PspN4I GGNNCC 2 cut(s) 73, 223
PspPI GGNCC 3 cut(s) 9, 222, 519
PstI CTGCAG 1 cut(s) 165
PvuII CAGCTG 1 cut(s) 236
RsaI GTAC 2 cut(s) 134, 284
RsaNI GTAC 2 cut(s) 133, 283
SacII CCGCGG 1 cut(s) 651
SatI GCNGC 7 cut(s) 75, 161, 164, 201, 204, 234, 648
Sau96I GGNCC 3 cut(s) 9, 222, 519
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 1 cut(s) 60
SduI GDGCHC 1 cut(s) 151
SexAI ACCWGGT 1 cut(s) 58
SfaNI GCATC 2 cut(s) 406, 713
SfcI CTRYAG 1 cut(s) 161
SfoI GGCGCC 1 cut(s) 73
Sfr303I CCGCGG 1 cut(s) 651
SgrBI CCGCGG 1 cut(s) 651
SinI GGWCC 1 cut(s) 519
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
Sse9I AATT 5 cut(s) 210, 303, 389, 554, 684
SsiI CCGC 6 cut(s) 75, 97, 129, 522, 648, 650
SspDI GGCGCC 1 cut(s) 71
SspMI CTAG 3 cut(s) 80, 420, 482
StyD4I CCNGG 1 cut(s) 58
TaaI ACNGT 1 cut(s) 153
TasI AATT 5 cut(s) 210, 303, 389, 554, 684
TatI WGTACW 1 cut(s) 282
TauI GCSGC 2 cut(s) 77, 650
TfiI GAWTC 3 cut(s) 86, 182, 292
TscAI CASTG 2 cut(s) 156, 634
TseFI GTSAC 2 cut(s) 448, 563
TseI GCWGC 5 cut(s) 160, 163, 200, 203, 233
Tsp45I GTSAC 2 cut(s) 448, 563
TspDTI ATGAA 3 cut(s) 302, 402, 459
TspGWI ACGGA 1 cut(s) 551
TspRI CASTG 2 cut(s) 156, 634
VpaK11BI GGWCC 1 cut(s) 519
XapI RAATTY 2 cut(s) 389, 554
XceI RCATGY 1 cut(s) 269
XmiI GTMKAC 1 cut(s) 499
XmnI GAANNNNTTC 1 cut(s) 710
XspI CTAG 3 cut(s) 80, 420, 482
Zsp2I ATGCAT 1 cut(s) 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.