Rh7DG137700

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0009216
Physical Location & Seq
Reverse (-)
11645090 .. 11647720
2631 bp
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UTR
Exon/CDS
Intron
Rh7DG137700.1

Sequence Viewer

Length: 648 bp
ATGAGACCTGAAATCGTGTTGTTTGGAGACTCGATAACAGAGCGGTCCTTCCAATCAGGAGGCTGGGGTGCCGCTCTTGCTGACACCTATTCTCGCAAGGCTGATGTCAAAGTTCGTGGCTATGGTGGGTACAACACCAGATGGGCGCTGTTCTTGTTGCATCAGATCTTTCCTCTGGATTCCAGAAAACCTCCTGCTGCTGCCACAATTTTCTTTGGGGCCAATGATGCAGCTATTTTGGGGAGAACAAGTGAACGGCAACATGTTCCTCTTGAAGAGTTCAAGGAGAATCTCAGAAAAATTGTTCTCCATTTGAAGGAGTGCAGTCCCACAATTCTGATTGTGCTTATCACTCCACCACCTGTGGATGAGGAAGGGCGTAATGAATATGCACGATCTTTGTATGGTGAGGATGCTAGGAAACTGCCAGAAAGGACAAATGAAGCAGCAGAAGTTTATGCAAAGAAGTGCCTTGAGCTAGCTGAGGAAATGGGTATCCGCTCCATCAATCTTTGGTCCAAGTTGCAGGAAACAGAGGGTTGGCAGAAGAAATTTCTAAGGTTTGTCATCTTTGAACCTTTAATTATCAAGTTTCTCGAACAATTCAACACTACTGTGATTCCATCCAGAATTTTACCCTTGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL Lipase_GDSL_2

Protein Analysis

215

Amino Acids

24.57

Weight (kDa)

7.75

Isoelectric Point (pI)

53.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 5 - 183 1.3e-27 GDSL-like Lipase/Acylhydrolase
Lipase_GDSL_2 PF13472 7 - 185 1.5e-19 GDSL-like Lipase/Acylhydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 68
AccBSI CCGCTC 3 cut(s) 43, 74, 501
AciI CCGC 3 cut(s) 43, 72, 499
AcsI RAATTY 2 cut(s) 551, 630
AfaI GTAC 1 cut(s) 131
AfiI CCNNNNNNNGG 1 cut(s) 316
AflIII ACRYGT 1 cut(s) 262
AgsI TTSAA 5 cut(s) 275, 283, 316, 575, 607
AleI CACNNNNGTG 1 cut(s) 614
AluBI AGCT 3 cut(s) 233, 478, 482
AluI AGCT 3 cut(s) 233, 478, 482
Alw26I GTCTC 1 cut(s) 21
AoxI GGCC 1 cut(s) 219
ApeKI GCWGC 4 cut(s) 197, 200, 230, 446
ApoI RAATTY 2 cut(s) 551, 630
AspLEI GCGC 1 cut(s) 148
AspS9I GGNCC 3 cut(s) 45, 219, 516
AsuHPI GGTGA 1 cut(s) 419
AsuNHI GCTAGC 1 cut(s) 478
AvaII GGWCC 2 cut(s) 45, 516
BanI GGYRCC 1 cut(s) 68
BbvCI CCTCAGC 1 cut(s) 483
BbvI GCAGC 4 cut(s) 184, 187, 242, 458
BccI CCATC 3 cut(s) 135, 512, 631
BceAI ACGGC 1 cut(s) 272
BciVI GTATCC 1 cut(s) 506
BcoDI GTCTC 1 cut(s) 21
BfaI CTAG 2 cut(s) 417, 479
BfoI RGCGCY 1 cut(s) 149
BfuI GTATCC 1 cut(s) 506
BglII AGATCT 1 cut(s) 165
BisI GCNGC 5 cut(s) 72, 198, 201, 231, 447
BlsI GCNGC 5 cut(s) 73, 199, 202, 232, 448
Bme18I GGWCC 2 cut(s) 45, 516
BmgT120I GGNCC 3 cut(s) 45, 219, 516
BmiI GGNNCC 2 cut(s) 70, 220
BmsI GCATC 3 cut(s) 169, 217, 403
BmtI GCTAGC 1 cut(s) 482
Bpu10I CCTNAGC 1 cut(s) 483
BpuEI CTTGAG 1 cut(s) 494
BsaXI ACNNNNNCTCC 2 cut(s) 235, 265
Bsc4I CCNNNNNNNGG 1 cut(s) 316
BseGI GGATG 3 cut(s) 373, 418, 623
BseLI CCNNNNNNNGG 1 cut(s) 316
BseMII CTCAG 2 cut(s) 307, 474
BseXI GCAGC 4 cut(s) 184, 187, 242, 458
BseYI CCCAGC 1 cut(s) 63
BsgI GTGCAG 1 cut(s) 343
BshFI GGCC 1 cut(s) 221
BshNI GGYRCC 1 cut(s) 68
BslFI GGGAC 1 cut(s) 312
BslI CCNNNNNNNGG 1 cut(s) 316
BsmAI GTCTC 1 cut(s) 21
BsmFI GGGAC 1 cut(s) 312
BsnI GGCC 1 cut(s) 221
Bsp143I GATC 2 cut(s) 165, 395
BspACI CCGC 3 cut(s) 43, 72, 499
BspANI GGCC 1 cut(s) 221
BspCNI CTCAG 2 cut(s) 306, 475
BspLI GGNNCC 2 cut(s) 70, 220
BspOI GCTAGC 1 cut(s) 482
BspT107I GGYRCC 1 cut(s) 68
BsrBI CCGCTC 3 cut(s) 43, 74, 501
BssMI GATC 2 cut(s) 165, 395
Bst4CI ACNGT 1 cut(s) 616
Bst6I CTCTTC 1 cut(s) 270
BstC8I GCNNGC 1 cut(s) 480
BstDEI CTNAG 3 cut(s) 293, 483, 557
BstF5I GGATG 3 cut(s) 373, 418, 623
BstH2I RGCGCY 1 cut(s) 149
BstHHI GCGC 1 cut(s) 148
BstKTI GATC 2 cut(s) 168, 398
BstMAI GTCTC 1 cut(s) 21
BstMBI GATC 2 cut(s) 165, 395
BstMWI GCNNNNNNNGC 2 cut(s) 77, 227
BstNSI RCATGY 1 cut(s) 266
BstV1I GCAGC 4 cut(s) 184, 187, 242, 458
BstX2I RGATCY 1 cut(s) 165
BstYI RGATCY 1 cut(s) 165
BsuI GTATCC 1 cut(s) 506
BsuRI GGCC 1 cut(s) 221
BtsCI GGATG 3 cut(s) 373, 418, 623
Cac8I GCNNGC 1 cut(s) 480
CfoI GCGC 1 cut(s) 148
Cfr13I GGNCC 3 cut(s) 45, 219, 516
Csp6I GTAC 1 cut(s) 130
CspCI CAANNNNNGTGG 2 cut(s) 97, 132
CviAII CATG 1 cut(s) 263
CviJI RGCY 7 cut(s) 63, 101, 120, 221, 233, 478, 482
CviKI_1 RGCY 7 cut(s) 63, 101, 120, 221, 233, 478, 482
CviQI GTAC 1 cut(s) 130
DdeI CTNAG 3 cut(s) 293, 483, 557
DpnI GATC 2 cut(s) 167, 397
DpnII GATC 2 cut(s) 165, 395
Eam1104I CTCTTC 1 cut(s) 270
EarI CTCTTC 1 cut(s) 270
Eco47I GGWCC 2 cut(s) 45, 516
FaeI CATG 1 cut(s) 266
FaiI YATR 5 cut(s) 123, 264, 390, 405, 459
FaqI GGGAC 1 cut(s) 312
FatI CATG 1 cut(s) 262
Fnu4HI GCNGC 5 cut(s) 72, 198, 201, 231, 447
FokI GGATG 3 cut(s) 380, 425, 610
Fsp4HI GCNGC 5 cut(s) 72, 198, 201, 231, 447
FspBI CTAG 2 cut(s) 417, 479
GlaI GCGC 1 cut(s) 147
GluI GCNGC 5 cut(s) 72, 198, 201, 231, 447
GsaI CCCAGC 1 cut(s) 67
HaeII RGCGCY 1 cut(s) 149
HaeIII GGCC 1 cut(s) 221
HhaI GCGC 1 cut(s) 148
Hin1II CATG 1 cut(s) 266
Hin6I GCGC 1 cut(s) 146
HinP1I GCGC 1 cut(s) 146
HinfI GANTC 4 cut(s) 29, 179, 289, 619
HphI GGTGA 1 cut(s) 419
Hpy166II GTNNAC 1 cut(s) 254
Hpy188I TCNGA 3 cut(s) 165, 296, 339
Hpy188III TCNNGA 6 cut(s) 57, 176, 183, 272, 596, 627
Hpy8I GTNNAC 1 cut(s) 254
HpyAV CCTTC 3 cut(s) 58, 310, 368
HpyCH4III ACNGT 1 cut(s) 616
HpyCH4V TGCA 7 cut(s) 160, 230, 324, 392, 461, 526, 643
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 227
HpyF3I CTNAG 3 cut(s) 293, 483, 557
Hsp92II CATG 1 cut(s) 266
HspAI GCGC 1 cut(s) 146
Kzo9I GATC 2 cut(s) 165, 395
LmnI GCTCC 1 cut(s) 506
Lsp1109I GCAGC 4 cut(s) 184, 187, 242, 458
LweI GCATC 3 cut(s) 169, 217, 403
MaeI CTAG 2 cut(s) 417, 479
MalI GATC 2 cut(s) 167, 397
MbiI CCGCTC 3 cut(s) 43, 74, 501
MboI GATC 2 cut(s) 165, 395
MboII GAAGA 2 cut(s) 287, 559
MflI RGATCY 1 cut(s) 165
MluCI AATT 7 cut(s) 207, 300, 333, 551, 582, 602, 630
MlyI GAGTC 1 cut(s) 23
MnlI CCTC 8 cut(s) 53, 183, 201, 279, 364, 403, 478, 529
MseI TTAA 1 cut(s) 581
MslI CAYNNNNRTG 1 cut(s) 614
MwoI GCNNNNNNNGC 2 cut(s) 77, 227
NdeII GATC 2 cut(s) 165, 395
NheI GCTAGC 1 cut(s) 478
NlaIII CATG 1 cut(s) 266
NlaIV GGNNCC 2 cut(s) 70, 220
NspI RCATGY 1 cut(s) 266
OliI CACNNNNGTG 1 cut(s) 614
PciI ACATGT 1 cut(s) 262
PfeI GAWTC 3 cut(s) 179, 289, 619
PkrI GCNGC 5 cut(s) 73, 199, 202, 232, 448
PleI GAGTC 1 cut(s) 23
PpsI GAGTC 1 cut(s) 23
PscI ACATGT 1 cut(s) 262
PspFI CCCAGC 1 cut(s) 63
PspN4I GGNNCC 2 cut(s) 70, 220
PspPI GGNCC 3 cut(s) 45, 219, 516
PsuI RGATCY 1 cut(s) 165
RsaI GTAC 1 cut(s) 131
RsaNI GTAC 1 cut(s) 130
RseI CAYNNNNRTG 1 cut(s) 614
SaqAI TTAA 1 cut(s) 581
SatI GCNGC 5 cut(s) 72, 198, 201, 231, 447
Sau3AI GATC 2 cut(s) 165, 395
Sau96I GGNCC 3 cut(s) 45, 219, 516
SchI GAGTC 1 cut(s) 23
SetI ASST 9 cut(s) 10, 89, 193, 235, 364, 480, 484, 563, 580
SfaNI GCATC 3 cut(s) 169, 217, 403
SinI GGWCC 2 cut(s) 45, 516
SmiMI CAYNNNNRTG 1 cut(s) 614
SmlI CTYRAG 1 cut(s) 473
SmoI CTYRAG 1 cut(s) 473
Sse9I AATT 7 cut(s) 207, 300, 333, 551, 582, 602, 630
SsiI CCGC 3 cut(s) 43, 72, 499
SspMI CTAG 2 cut(s) 417, 479
TaaI ACNGT 1 cut(s) 616
TaqI TCGA 2 cut(s) 32, 597
TasI AATT 7 cut(s) 207, 300, 333, 551, 582, 602, 630
TauI GCSGC 1 cut(s) 74
TfiI GAWTC 3 cut(s) 179, 289, 619
Tru1I TTAA 1 cut(s) 581
Tru9I TTAA 1 cut(s) 581
TseI GCWGC 4 cut(s) 197, 200, 230, 446
TspDTI ATGAA 2 cut(s) 399, 456
VpaK11BI GGWCC 2 cut(s) 45, 516
XapI RAATTY 2 cut(s) 551, 630
XceI RCATGY 1 cut(s) 266
XspI CTAG 2 cut(s) 417, 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.