ATCG01000

BEST Arabidopsis thaliana protein match is Ycf1 protein (TAIR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
Pt
OG0011870
Physical Location & Seq
Forward (+)
109405 .. 110436
1032 bp
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UTR
Exon/CDS
Intron
ATCG01000.1

Sequence Viewer

Length: 1032 bp
GTGATGGTTTTTCAATCTTTTATACTAGGTAATCTAGTATCCTTATGCATGAAGATAATAAATTCGGTCGTTGTGGTCGGACTCTATTATGGATTTCTGACCACATTTTCCATAGGGCCCTCTTATCTCTTCCTTCTCCGAGCTCGGGTTATGGACGAAGGAGAAGAAGGAACCGAGAAGAAAGTATCAGCAACAACAGGTTTTATTGCGGGACAGCTCATGATGTTCATATCGATCTATTATGCGCCTCTGCATTTAGCATTGGGTAGACCTCATACAATAACTGTCCTAGCTCTACCGTATCTTTTGTTTCATTTCTTCTGGAACAATCACAAACACTTTTTTGATTATGGATCTACTACCAGAAATGAAATGCGTAATCTTCGCATTCAATGTGTATTCCTGAATAATCTCATTTTTCAATTATTCAACCATTTCATTTTACCAAGTTCAATGTTAGCCAGATTAGTCAACATTTATATGTTTCGATGCAACAACAAGATGTTATTTGTAACAAGTAGTTTTGTTGGTTGGTTAATTGGTCACATTTTATTCATGAAATGGGTTGGATTGGTATTAGTCTGGATACAGCAAAATAATTCTATTAGGTCTAATGTAGTTATTAGATCTAATAAGTATAAGTTCCTTGTGTCAGAATTGAGAAATTCTATGGCTCGAATCTTTAGTATTCTCTTATTTATTACCTGTGTCTACTATTTAGGCAGAATACCATCACCCATTTTTACTAAGAAACTTAAAGGAACCTCAGAAACGGGTGGGACTAAACAGGACCAAGAGGTATCCACCGAAGAAGCTCCTTTTCCTTCTCTTTTTTCGGAAGAAGGGGAAGATCTGGACAAAATCGATGAAATGGAAGAAATCCGAGTGAATGGAAAAGACAAAATTAATAAGGATGATGAATTCCACGTTCGAACATACTATAACTATAAAACTGTTTCTGAAAATCTATATGGAAATAAAGAAAATTCGAATTTAGAATTTTTCAAAATAAAAAAAAAAGAGGATCATTAA

Protein Analysis

343

Amino Acids

39.89

Weight (kDa)

8.96

Isoelectric Point (pI)

42.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ycf1 PF05758 22 - 343 9.2e-189 Ycf1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 268, 711
AciI CCGC 1 cut(s) 209
AclWI GGATC 1 cut(s) 361
AcsI RAATTY 6 cut(s) 61, 664, 920, 985, 991, 998
AfiI CCNNNNNNNGG 1 cut(s) 145
AgsI TTSAA 6 cut(s) 14, 392, 422, 430, 453, 1006
AluBI AGCT 4 cut(s) 143, 217, 293, 815
AluI AGCT 4 cut(s) 143, 217, 293, 815
Alw21I GWGCWC 1 cut(s) 145
AlwI GGATC 1 cut(s) 361
Ama87I CYCGRG 1 cut(s) 144
AoxI GGCC 1 cut(s) 116
ApaI GGGCCC 1 cut(s) 120
ApoI RAATTY 6 cut(s) 61, 664, 920, 985, 991, 998
AseI ATTAAT 1 cut(s) 906
AspLEI GCGC 1 cut(s) 247
AspS9I GGNCC 3 cut(s) 116, 117, 790
AsuHPI GGTGA 1 cut(s) 726
AsuII TTCGAA 2 cut(s) 931, 989
AvaI CYCGRG 1 cut(s) 144
AvaII GGWCC 1 cut(s) 790
BaeGI GKGCMC 1 cut(s) 120
BanII GRGCYC 2 cut(s) 120, 145
Bbv12I GWGCWC 1 cut(s) 145
BccI CCATC 1 cut(s) 739
BciVI GTATCC 3 cut(s) 49, 579, 811
BfaI CTAG 3 cut(s) 26, 35, 290
BfuI GTATCC 3 cut(s) 49, 579, 811
BglII AGATCT 2 cut(s) 626, 850
Bme18I GGWCC 1 cut(s) 790
BmeT110I CYCGRG 1 cut(s) 144
BmgT120I GGNCC 3 cut(s) 116, 117, 790
BmiI GGNNCC 3 cut(s) 118, 172, 763
BmsI GCATC 1 cut(s) 479
Bpu14I TTCGAA 2 cut(s) 931, 989
Bsa29I ATCGAT 2 cut(s) 233, 864
Bsc4I CCNNNNNNNGG 1 cut(s) 145
BseCI ATCGAT 2 cut(s) 233, 864
BseGI GGATG 1 cut(s) 919
BseLI CCNNNNNNNGG 1 cut(s) 145
BseMII CTCAG 1 cut(s) 780
BseSI GKGCMC 1 cut(s) 120
Bsh1285I CGRYCG 1 cut(s) 69
BshFI GGCC 1 cut(s) 118
BshVI ATCGAT 2 cut(s) 233, 864
BsiEI CGRYCG 1 cut(s) 69
BsiHKAI GWGCWC 1 cut(s) 145
BsiHKCI CYCGRG 1 cut(s) 144
BslFI GGGAC 2 cut(s) 225, 793
BslI CCNNNNNNNGG 1 cut(s) 145
BsmFI GGGAC 2 cut(s) 225, 793
BsmI GAATGC 1 cut(s) 387
BsnI GGCC 1 cut(s) 118
BsoBI CYCGRG 1 cut(s) 144
Bsp119I TTCGAA 2 cut(s) 931, 989
Bsp120I GGGCCC 1 cut(s) 116
Bsp1286I GDGCHC 2 cut(s) 120, 145
Bsp143I GATC 5 cut(s) 234, 353, 626, 850, 1024
BspACI CCGC 1 cut(s) 209
BspANI GGCC 1 cut(s) 118
BspCNI CTCAG 1 cut(s) 779
BspDI ATCGAT 2 cut(s) 233, 864
BspHI TCATGA 2 cut(s) 219, 555
BspLI GGNNCC 3 cut(s) 118, 172, 763
BspPI GGATC 1 cut(s) 361
BspT104I TTCGAA 2 cut(s) 931, 989
BssMI GATC 5 cut(s) 234, 353, 626, 850, 1024
Bst4CI ACNGT 3 cut(s) 286, 300, 955
Bst6I CTCTTC 1 cut(s) 134
BstBI TTCGAA 2 cut(s) 931, 989
BstDEI CTNAG 2 cut(s) 747, 766
BstF5I GGATG 1 cut(s) 919
BstHHI GCGC 1 cut(s) 247
BstKTI GATC 5 cut(s) 237, 356, 629, 853, 1027
BstMBI GATC 5 cut(s) 234, 353, 626, 850, 1024
BstMCI CGRYCG 1 cut(s) 69
BstSLI GKGCMC 1 cut(s) 120
BstX2I RGATCY 3 cut(s) 353, 626, 850
BstYI RGATCY 3 cut(s) 353, 626, 850
Bsu15I ATCGAT 2 cut(s) 233, 864
BsuI GTATCC 3 cut(s) 49, 579, 811
BsuRI GGCC 1 cut(s) 118
BsuTUI ATCGAT 2 cut(s) 233, 864
BtsCI GGATG 1 cut(s) 919
CciI TCATGA 2 cut(s) 219, 555
CfoI GCGC 1 cut(s) 247
Cfr13I GGNCC 3 cut(s) 116, 117, 790
ClaI ATCGAT 2 cut(s) 233, 864
CviAII CATG 3 cut(s) 49, 220, 556
CviJI RGCY 7 cut(s) 118, 143, 217, 293, 461, 674, 815
CviKI_1 RGCY 7 cut(s) 118, 143, 217, 293, 461, 674, 815
DdeI CTNAG 2 cut(s) 747, 766
DpnI GATC 5 cut(s) 236, 355, 628, 852, 1026
DpnII GATC 5 cut(s) 234, 353, 626, 850, 1024
Eam1104I CTCTTC 1 cut(s) 134
EarI CTCTTC 1 cut(s) 134
Ecl136II GAGCTC 1 cut(s) 143
Eco24I GRGCYC 2 cut(s) 120, 145
Eco47I GGWCC 1 cut(s) 790
Eco53kI GAGCTC 1 cut(s) 143
Eco88I CYCGRG 1 cut(s) 144
EcoICRI GAGCTC 1 cut(s) 143
EcoO109I RGGNCCY 2 cut(s) 116, 117
EcoRI GAATTC 1 cut(s) 920
EcoT22I ATGCAT 1 cut(s) 50
EcoT38I GRGCYC 2 cut(s) 120, 145
FaeI CATG 3 cut(s) 52, 223, 559
FaqI GGGAC 2 cut(s) 225, 793
FatI CATG 3 cut(s) 48, 219, 555
FauI CCCGC 1 cut(s) 202
FblI GTMKAC 2 cut(s) 268, 711
FokI GGATG 1 cut(s) 926
FriOI GRGCYC 2 cut(s) 120, 145
FspBI CTAG 3 cut(s) 26, 35, 290
GlaI GCGC 1 cut(s) 246
HaeIII GGCC 1 cut(s) 118
HhaI GCGC 1 cut(s) 247
Hin1II CATG 3 cut(s) 52, 223, 559
Hin6I GCGC 1 cut(s) 245
HinP1I GCGC 1 cut(s) 245
HincII GTYRAC 1 cut(s) 472
HindII GTYRAC 1 cut(s) 472
HinfI GANTC 2 cut(s) 81, 678
HphI GGTGA 1 cut(s) 726
Hpy166II GTNNAC 3 cut(s) 269, 472, 712
Hpy188I TCNGA 8 cut(s) 80, 99, 140, 655, 769, 838, 884, 961
Hpy188III TCNNGA 6 cut(s) 220, 322, 403, 556, 583, 854
Hpy8I GTNNAC 3 cut(s) 269, 472, 712
HpyAV CCTTC 5 cut(s) 143, 152, 161, 834, 836
HpyCH4III ACNGT 3 cut(s) 286, 300, 955
HpyCH4IV ACGT 1 cut(s) 927
HpyCH4V TGCA 3 cut(s) 48, 253, 492
HpyF3I CTNAG 2 cut(s) 747, 766
HpySE526I ACGT 1 cut(s) 927
Hsp92II CATG 3 cut(s) 52, 223, 559
HspAI GCGC 1 cut(s) 245
Kzo9I GATC 5 cut(s) 234, 353, 626, 850, 1024
LmnI GCTCC 1 cut(s) 820
LpnPI CCDG 9 cut(s) 183, 307, 376, 416, 475, 568, 718, 773, 839
LweI GCATC 1 cut(s) 479
MaeI CTAG 3 cut(s) 26, 35, 290
MaeII ACGT 1 cut(s) 927
MaeIII GTNAC 2 cut(s) 511, 542
MalI GATC 5 cut(s) 236, 355, 628, 852, 1026
MboI GATC 5 cut(s) 234, 353, 626, 850, 1024
MflI RGATCY 3 cut(s) 353, 626, 850
MhlI GDGCHC 2 cut(s) 120, 145
MlyI GAGTC 1 cut(s) 75
MmeI TCCRAC 2 cut(s) 58, 547
MnlI CCTC 6 cut(s) 130, 258, 282, 775, 790, 1015
Mph1103I ATGCAT 1 cut(s) 50
MseI TTAA 4 cut(s) 536, 756, 906, 1030
MslI CAYNNNNRTG 1 cut(s) 479
Mva1269I GAATGC 1 cut(s) 387
NdeII GATC 5 cut(s) 234, 353, 626, 850, 1024
NlaIII CATG 3 cut(s) 52, 223, 559
NlaIV GGNNCC 3 cut(s) 118, 172, 763
NmuCI GTSAC 1 cut(s) 542
NsiI ATGCAT 1 cut(s) 50
NspV TTCGAA 2 cut(s) 931, 989
PagI TCATGA 2 cut(s) 219, 555
PctI GAATGC 1 cut(s) 387
PfeI GAWTC 1 cut(s) 678
PleI GAGTC 1 cut(s) 75
PpsI GAGTC 1 cut(s) 75
PshBI ATTAAT 1 cut(s) 906
Psp124BI GAGCTC 1 cut(s) 145
PspN4I GGNNCC 3 cut(s) 118, 172, 763
PspOMI GGGCCC 1 cut(s) 116
PspPI GGNCC 3 cut(s) 116, 117, 790
PsuI RGATCY 3 cut(s) 353, 626, 850
RseI CAYNNNNRTG 1 cut(s) 479
SacI GAGCTC 1 cut(s) 145
SaqAI TTAA 4 cut(s) 536, 756, 906, 1030
Sau3AI GATC 5 cut(s) 234, 353, 626, 850, 1024
Sau96I GGNCC 3 cut(s) 116, 117, 790
SchI GAGTC 1 cut(s) 75
SduI GDGCHC 2 cut(s) 120, 145
SfaNI GCATC 1 cut(s) 479
SfuI TTCGAA 2 cut(s) 931, 989
SinI GGWCC 1 cut(s) 790
SmiMI CAYNNNNRTG 1 cut(s) 479
SsiI CCGC 1 cut(s) 209
SspMI CTAG 3 cut(s) 26, 35, 290
SstI GAGCTC 1 cut(s) 145
TaaI ACNGT 3 cut(s) 286, 300, 955
TaiI ACGT 1 cut(s) 930
TaqI TCGA 6 cut(s) 233, 487, 676, 864, 931, 989
TaqII GACCGA 1 cut(s) 55
TfiI GAWTC 1 cut(s) 678
Tru1I TTAA 4 cut(s) 536, 756, 906, 1030
Tru9I TTAA 4 cut(s) 536, 756, 906, 1030
TseFI GTSAC 1 cut(s) 542
Tsp45I GTSAC 1 cut(s) 542
TspDTI ATGAA 9 cut(s) 65, 217, 302, 384, 427, 544, 572, 882, 933
VpaK11BI GGWCC 1 cut(s) 790
VspI ATTAAT 1 cut(s) 906
XapI RAATTY 6 cut(s) 61, 664, 920, 985, 991, 998
XmiI GTMKAC 2 cut(s) 268, 711
XspI CTAG 3 cut(s) 26, 35, 290
Zsp2I ATGCAT 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.