RchiOBHm_Chr7g0221181

Involved in protein precursor import into chloroplasts. May be part of an intermediate translocation complex acting as a protein-conducting channel at the inner envelope

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0011870
Physical Location & Seq
Forward (+)
42046215 .. 42046934
720 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19789

Sequence Viewer

Length: 720 bp
ATGATTTTAAAATCTTTTATATTAGGGAATCTAGTATCCTTATGCATGAAGATAATCAATTCGGTCGTTGTGGTCAGACTCTATTATGGATTTCTGACCACATTCTCTATAGGGCCCTCTTATCTCTTCCTTCTCCGAGCTCAGGTTATGGAAGAAGGAGAAGAAGGAACCGAGAAGAAAGTATCAGTAACAACCGGTTTTATTACGGGACAGCTCATGATGTTCATATCGATCTATTATGTACCTTTGCATCTAACATTGGGTAGACCTCATATAATAACTGTCCTAGCTCTACCGTATCTTTTGTTTCATTTCTTCTGGAACAATCACAAACACTTTTTTGATTATGGATCTACTACCAGAAATTCAATGCGTAATCTTAGCATTCAATGTTTATTCCTGAATAATCTCATTGTTCAATTATTCAACCATTTCATTTTACCAAGTTCAATGTTAGTCAGATTAGTCAATATTTCTATGTTTCGATGCAACAACAAGATGTTATTTGTAACAAGTAGTTTTGTTGGTTGGTTAATTGGTCACATTTTATTCATGAAATGGGTTGGATTGGTATTAGGCTGGATACAGCAAAATAATTCTATTAGATCTAATGTACTTATTAGATCGAATAAGTACCTTGTGTCAGAATTGAGAAATTCTATGGCTCGAATCTTTAGTATTCTCTTATTTATTGTTATTTTGGGTGGAGTTGAAATTTAG

Protein Analysis

239

Amino Acids

27.56

Weight (kDa)

9.92

Isoelectric Point (pI)

42.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ycf1 PF05758 21 - 233 2.6e-141 Ycf1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 265
AclWI GGATC 1 cut(s) 358
AcsI RAATTY 3 cut(s) 364, 655, 714
AfaI GTAC 3 cut(s) 243, 615, 635
AfiI CCNNNNNNNGG 1 cut(s) 142
AgeI ACCGGT 1 cut(s) 194
AgsI TTSAA 6 cut(s) 369, 389, 419, 427, 450, 713
AluBI AGCT 3 cut(s) 140, 214, 290
AluI AGCT 3 cut(s) 140, 214, 290
Alw21I GWGCWC 1 cut(s) 142
AlwI GGATC 1 cut(s) 358
AoxI GGCC 1 cut(s) 113
ApaI GGGCCC 1 cut(s) 117
ApoI RAATTY 3 cut(s) 364, 655, 714
AsiGI ACCGGT 1 cut(s) 194
AspS9I GGNCC 2 cut(s) 113, 114
BaeGI GKGCMC 1 cut(s) 117
BanII GRGCYC 2 cut(s) 117, 142
Bbv12I GWGCWC 1 cut(s) 142
BciVI GTATCC 2 cut(s) 46, 576
BfaI CTAG 2 cut(s) 32, 287
BfmI CTRYAG 1 cut(s) 108
BfuI GTATCC 2 cut(s) 46, 576
BglII AGATCT 1 cut(s) 605
BmgT120I GGNCC 2 cut(s) 113, 114
BmiI GGNNCC 2 cut(s) 115, 169
BmsI GCATC 2 cut(s) 259, 476
Bpu10I CCTNAGC 1 cut(s) 141
Bsa29I ATCGAT 1 cut(s) 230
BsaWI WCCGGW 1 cut(s) 194
Bsc4I CCNNNNNNNGG 1 cut(s) 142
Bse118I RCCGGY 1 cut(s) 194
BseCI ATCGAT 1 cut(s) 230
BseLI CCNNNNNNNGG 1 cut(s) 142
BseMII CTCAG 1 cut(s) 155
BseSI GKGCMC 1 cut(s) 117
Bsh1285I CGRYCG 1 cut(s) 66
BshFI GGCC 1 cut(s) 115
BshTI ACCGGT 1 cut(s) 194
BshVI ATCGAT 1 cut(s) 230
BsiEI CGRYCG 1 cut(s) 66
BsiHKAI GWGCWC 1 cut(s) 142
BsiSI CCGG 1 cut(s) 195
BslFI GGGAC 1 cut(s) 222
BslI CCNNNNNNNGG 1 cut(s) 142
BsmFI GGGAC 1 cut(s) 222
BsmI GAATGC 1 cut(s) 384
BsnI GGCC 1 cut(s) 115
Bsp120I GGGCCC 1 cut(s) 113
Bsp1286I GDGCHC 2 cut(s) 117, 142
Bsp143I GATC 4 cut(s) 231, 350, 605, 623
BspANI GGCC 1 cut(s) 115
BspCNI CTCAG 1 cut(s) 154
BspDI ATCGAT 1 cut(s) 230
BspHI TCATGA 2 cut(s) 216, 552
BspLI GGNNCC 2 cut(s) 115, 169
BspPI GGATC 1 cut(s) 358
BsrFI RCCGGY 1 cut(s) 194
BssAI RCCGGY 1 cut(s) 194
BssMI GATC 4 cut(s) 231, 350, 605, 623
Bst4CI ACNGT 2 cut(s) 283, 297
Bst6I CTCTTC 1 cut(s) 131
BstDEI CTNAG 2 cut(s) 141, 380
BstKTI GATC 4 cut(s) 234, 353, 608, 626
BstMBI GATC 4 cut(s) 231, 350, 605, 623
BstMCI CGRYCG 1 cut(s) 66
BstSFI CTRYAG 1 cut(s) 108
BstSLI GKGCMC 1 cut(s) 117
BstX2I RGATCY 2 cut(s) 350, 605
BstYI RGATCY 2 cut(s) 350, 605
Bsu15I ATCGAT 1 cut(s) 230
BsuI GTATCC 2 cut(s) 46, 576
BsuRI GGCC 1 cut(s) 115
BsuTUI ATCGAT 1 cut(s) 230
CciI TCATGA 2 cut(s) 216, 552
Cfr10I RCCGGY 1 cut(s) 194
Cfr13I GGNCC 2 cut(s) 113, 114
ClaI ATCGAT 1 cut(s) 230
Csp6I GTAC 3 cut(s) 242, 614, 634
CspAI ACCGGT 1 cut(s) 194
CviAII CATG 3 cut(s) 46, 217, 553
CviJI RGCY 6 cut(s) 115, 140, 214, 290, 579, 665
CviKI_1 RGCY 6 cut(s) 115, 140, 214, 290, 579, 665
CviQI GTAC 3 cut(s) 242, 614, 634
DdeI CTNAG 2 cut(s) 141, 380
DpnI GATC 4 cut(s) 233, 352, 607, 625
DpnII GATC 4 cut(s) 231, 350, 605, 623
DraI TTTAAA 1 cut(s) 9
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
Ecl136II GAGCTC 1 cut(s) 140
Eco24I GRGCYC 2 cut(s) 117, 142
Eco53kI GAGCTC 1 cut(s) 140
EcoICRI GAGCTC 1 cut(s) 140
EcoO109I RGGNCCY 2 cut(s) 113, 114
EcoT22I ATGCAT 1 cut(s) 47
EcoT38I GRGCYC 2 cut(s) 117, 142
FaeI CATG 3 cut(s) 49, 220, 556
FaqI GGGAC 1 cut(s) 222
FatI CATG 3 cut(s) 45, 216, 552
FblI GTMKAC 1 cut(s) 265
FriOI GRGCYC 2 cut(s) 117, 142
FspBI CTAG 2 cut(s) 32, 287
HaeIII GGCC 1 cut(s) 115
HapII CCGG 1 cut(s) 195
Hin1II CATG 3 cut(s) 49, 220, 556
HinfI GANTC 3 cut(s) 28, 78, 669
HpaII CCGG 1 cut(s) 195
Hpy166II GTNNAC 1 cut(s) 266
Hpy188I TCNGA 5 cut(s) 77, 96, 137, 461, 646
Hpy188III TCNNGA 4 cut(s) 217, 319, 400, 553
Hpy8I GTNNAC 1 cut(s) 266
HpyAV CCTTC 3 cut(s) 140, 149, 158
HpyCH4III ACNGT 2 cut(s) 283, 297
HpyCH4V TGCA 3 cut(s) 45, 250, 489
HpyF3I CTNAG 2 cut(s) 141, 380
Hsp92II CATG 3 cut(s) 49, 220, 556
Kzo9I GATC 4 cut(s) 231, 350, 605, 623
LpnPI CCDG 6 cut(s) 128, 208, 304, 373, 413, 565
LweI GCATC 2 cut(s) 259, 476
MaeI CTAG 2 cut(s) 32, 287
MaeIII GTNAC 3 cut(s) 187, 508, 539
MalI GATC 4 cut(s) 233, 352, 607, 625
MboI GATC 4 cut(s) 231, 350, 605, 623
MboII GAAGA 6 cut(s) 61, 118, 164, 173, 187, 307
MflI RGATCY 2 cut(s) 350, 605
MhlI GDGCHC 2 cut(s) 117, 142
MluCI AATT 8 cut(s) 58, 364, 419, 534, 595, 647, 655, 714
MlyI GAGTC 1 cut(s) 72
MmeI TCCRAC 1 cut(s) 544
MnlI CCTC 2 cut(s) 127, 279
Mph1103I ATGCAT 1 cut(s) 47
MseI TTAA 2 cut(s) 8, 533
MspI CCGG 1 cut(s) 195
Mva1269I GAATGC 1 cut(s) 384
NdeII GATC 4 cut(s) 231, 350, 605, 623
NlaIII CATG 3 cut(s) 49, 220, 556
NlaIV GGNNCC 2 cut(s) 115, 169
NmuCI GTSAC 1 cut(s) 539
NsiI ATGCAT 1 cut(s) 47
PagI TCATGA 2 cut(s) 216, 552
PctI GAATGC 1 cut(s) 384
PfeI GAWTC 2 cut(s) 28, 669
PinAI ACCGGT 1 cut(s) 194
PleI GAGTC 1 cut(s) 72
PpsI GAGTC 1 cut(s) 72
Psp124BI GAGCTC 1 cut(s) 142
PspN4I GGNNCC 2 cut(s) 115, 169
PspOMI GGGCCC 1 cut(s) 113
PspPI GGNCC 2 cut(s) 113, 114
PsuI RGATCY 2 cut(s) 350, 605
RsaI GTAC 3 cut(s) 243, 615, 635
RsaNI GTAC 3 cut(s) 242, 614, 634
SacI GAGCTC 1 cut(s) 142
SaqAI TTAA 2 cut(s) 8, 533
Sau3AI GATC 4 cut(s) 231, 350, 605, 623
Sau96I GGNCC 2 cut(s) 113, 114
SchI GAGTC 1 cut(s) 72
SduI GDGCHC 2 cut(s) 117, 142
SetI ASST 7 cut(s) 142, 147, 216, 247, 271, 292, 639
SfaNI GCATC 2 cut(s) 259, 476
SfcI CTRYAG 1 cut(s) 108
Sse9I AATT 8 cut(s) 58, 364, 419, 534, 595, 647, 655, 714
SspI AATATT 1 cut(s) 472
SspMI CTAG 2 cut(s) 32, 287
SstI GAGCTC 1 cut(s) 142
TaaI ACNGT 2 cut(s) 283, 297
TaqI TCGA 4 cut(s) 230, 484, 626, 667
TaqII GACCGA 1 cut(s) 52
TasI AATT 8 cut(s) 58, 364, 419, 534, 595, 647, 655, 714
TatI WGTACW 1 cut(s) 613
TfiI GAWTC 2 cut(s) 28, 669
Tru1I TTAA 2 cut(s) 8, 533
Tru9I TTAA 2 cut(s) 8, 533
TseFI GTSAC 1 cut(s) 539
Tsp45I GTSAC 1 cut(s) 539
TspDTI ATGAA 6 cut(s) 62, 214, 299, 424, 541, 569
XapI RAATTY 3 cut(s) 364, 655, 714
XmiI GTMKAC 1 cut(s) 265
XspI CTAG 2 cut(s) 32, 287
Zsp2I ATGCAT 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.