RchiOBHm_Chr4g0392781

Involved in protein precursor import into chloroplasts. May be part of an intermediate translocation complex acting as a protein-conducting channel at the inner envelope

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0011870
Physical Location & Seq
Forward (+)
8281289 .. 8281777
489 bp
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UTR
Exon/CDS
Intron
PRQ36545

Sequence Viewer

Length: 489 bp
ATGCATGAAGATAATCAATTCGGTCGTTGTGGTCGGACTCTATTATGGATTTCTGACCACATTCCCATAGGGCCCTCTTATCTCTTCCTTCTCAGAGCTCGGGTTATGGAAGAAGGAGAAGAAGGAACTGAGAAGAAAGTATCAGCAACAACTGGTTTTATTACGGGACAGCTCATGATGTTCATATTGATCTATTATGCGCCTCTGCATCTAGCATTGGGTAGACCTCATACAATAACTATCCAAGCTCTACCGTATCTTTTGTTTCATTTCTTCTGGAACAATCACAAACACTTTTTTGATTATGGATCTACTACCAGAAATTCAATGCATAATCTTAGCATTCAATGTTTATTCCTGAATAATCTCATTTTTCAATTATTCAACCATTTCATTTTACCAAGTTCAATGTTAGTCAGATTAGTCAACATTTATATGTTTCGATGCAACAACAAGATGTTATTTGTAACAAGTAGTTTTGTTGGTTAG

Protein Analysis

162

Amino Acids

18.93

Weight (kDa)

8.89

Isoelectric Point (pI)

49.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ycf1 PF05758 22 - 162 3.9e-95 Ycf1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 223
AclWI GGATC 1 cut(s) 316
AcsI RAATTY 1 cut(s) 322
AgsI TTSAA 5 cut(s) 327, 347, 377, 385, 408
AluBI AGCT 3 cut(s) 98, 172, 248
AluI AGCT 3 cut(s) 98, 172, 248
Alw21I GWGCWC 1 cut(s) 100
AlwI GGATC 1 cut(s) 316
Ama87I CYCGRG 1 cut(s) 99
AoxI GGCC 1 cut(s) 71
ApaI GGGCCC 1 cut(s) 75
ApoI RAATTY 1 cut(s) 322
AspLEI GCGC 1 cut(s) 202
AspS9I GGNCC 2 cut(s) 71, 72
AvaI CYCGRG 1 cut(s) 99
BaeGI GKGCMC 1 cut(s) 75
BanII GRGCYC 2 cut(s) 75, 100
Bbv12I GWGCWC 1 cut(s) 100
BfaI CTAG 1 cut(s) 212
BmeT110I CYCGRG 1 cut(s) 99
BmgT120I GGNCC 2 cut(s) 71, 72
BmiI GGNNCC 1 cut(s) 73
BmsI GCATC 2 cut(s) 217, 434
Bse1I ACTGG 1 cut(s) 157
BseMII CTCAG 2 cut(s) 106, 120
BseNI ACTGG 1 cut(s) 157
BseSI GKGCMC 1 cut(s) 75
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 1 cut(s) 73
BsiEI CGRYCG 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 100
BsiHKCI CYCGRG 1 cut(s) 99
BslFI GGGAC 1 cut(s) 180
BsmFI GGGAC 1 cut(s) 180
BsmI GAATGC 1 cut(s) 342
BsnI GGCC 1 cut(s) 73
BsoBI CYCGRG 1 cut(s) 99
Bsp120I GGGCCC 1 cut(s) 71
Bsp1286I GDGCHC 2 cut(s) 75, 100
Bsp143I GATC 2 cut(s) 189, 308
BspANI GGCC 1 cut(s) 73
BspCNI CTCAG 2 cut(s) 105, 121
BspHI TCATGA 1 cut(s) 174
BspLI GGNNCC 1 cut(s) 73
BspPI GGATC 1 cut(s) 316
BsrI ACTGG 1 cut(s) 157
BssMI GATC 2 cut(s) 189, 308
Bst4CI ACNGT 1 cut(s) 255
Bst6I CTCTTC 1 cut(s) 89
BstDEI CTNAG 3 cut(s) 92, 129, 338
BstHHI GCGC 1 cut(s) 202
BstKTI GATC 2 cut(s) 192, 311
BstMBI GATC 2 cut(s) 189, 308
BstMCI CGRYCG 1 cut(s) 25
BstSLI GKGCMC 1 cut(s) 75
BstX2I RGATCY 1 cut(s) 308
BstYI RGATCY 1 cut(s) 308
BsuRI GGCC 1 cut(s) 73
CciI TCATGA 1 cut(s) 174
CfoI GCGC 1 cut(s) 202
Cfr13I GGNCC 2 cut(s) 71, 72
CviAII CATG 2 cut(s) 5, 175
CviJI RGCY 4 cut(s) 73, 98, 172, 248
CviKI_1 RGCY 4 cut(s) 73, 98, 172, 248
DdeI CTNAG 3 cut(s) 92, 129, 338
DpnI GATC 2 cut(s) 191, 310
DpnII GATC 2 cut(s) 189, 308
Eam1104I CTCTTC 1 cut(s) 89
EarI CTCTTC 1 cut(s) 89
Ecl136II GAGCTC 1 cut(s) 98
Eco24I GRGCYC 2 cut(s) 75, 100
Eco53kI GAGCTC 1 cut(s) 98
Eco88I CYCGRG 1 cut(s) 99
EcoICRI GAGCTC 1 cut(s) 98
EcoO109I RGGNCCY 2 cut(s) 71, 72
EcoT22I ATGCAT 2 cut(s) 6, 333
EcoT38I GRGCYC 2 cut(s) 75, 100
FaeI CATG 2 cut(s) 8, 178
FaqI GGGAC 1 cut(s) 180
FatI CATG 2 cut(s) 4, 174
FblI GTMKAC 1 cut(s) 223
FriOI GRGCYC 2 cut(s) 75, 100
FspBI CTAG 1 cut(s) 212
GlaI GCGC 1 cut(s) 201
HaeIII GGCC 1 cut(s) 73
HhaI GCGC 1 cut(s) 202
Hin1II CATG 2 cut(s) 8, 178
Hin6I GCGC 1 cut(s) 200
HinP1I GCGC 1 cut(s) 200
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HinfI GANTC 1 cut(s) 37
Hpy166II GTNNAC 2 cut(s) 224, 427
Hpy188I TCNGA 4 cut(s) 36, 55, 95, 419
Hpy188III TCNNGA 3 cut(s) 175, 277, 358
Hpy8I GTNNAC 2 cut(s) 224, 427
HpyAV CCTTC 3 cut(s) 98, 107, 116
HpyCH4III ACNGT 1 cut(s) 255
HpyCH4V TGCA 4 cut(s) 4, 208, 331, 447
HpyF3I CTNAG 3 cut(s) 92, 129, 338
Hsp92II CATG 2 cut(s) 8, 178
HspAI GCGC 1 cut(s) 200
Kzo9I GATC 2 cut(s) 189, 308
LpnPI CCDG 4 cut(s) 138, 262, 331, 371
LweI GCATC 2 cut(s) 217, 434
MaeI CTAG 1 cut(s) 212
MaeIII GTNAC 1 cut(s) 466
MalI GATC 2 cut(s) 191, 310
MboI GATC 2 cut(s) 189, 308
MboII GAAGA 6 cut(s) 20, 76, 122, 131, 145, 265
MflI RGATCY 1 cut(s) 308
MhlI GDGCHC 2 cut(s) 75, 100
MluCI AATT 3 cut(s) 17, 322, 377
MlyI GAGTC 1 cut(s) 31
MmeI TCCRAC 1 cut(s) 14
MnlI CCTC 3 cut(s) 85, 213, 237
Mph1103I ATGCAT 2 cut(s) 6, 333
MslI CAYNNNNRTG 1 cut(s) 434
Mva1269I GAATGC 1 cut(s) 342
NdeII GATC 2 cut(s) 189, 308
NlaIII CATG 2 cut(s) 8, 178
NlaIV GGNNCC 1 cut(s) 73
NsiI ATGCAT 2 cut(s) 6, 333
PagI TCATGA 1 cut(s) 174
PctI GAATGC 1 cut(s) 342
PleI GAGTC 1 cut(s) 31
PpsI GAGTC 1 cut(s) 31
Psp124BI GAGCTC 1 cut(s) 100
PspN4I GGNNCC 1 cut(s) 73
PspOMI GGGCCC 1 cut(s) 71
PspPI GGNCC 2 cut(s) 71, 72
PsuI RGATCY 1 cut(s) 308
RseI CAYNNNNRTG 1 cut(s) 434
SacI GAGCTC 1 cut(s) 100
Sau3AI GATC 2 cut(s) 189, 308
Sau96I GGNCC 2 cut(s) 71, 72
SchI GAGTC 1 cut(s) 31
SduI GDGCHC 2 cut(s) 75, 100
SetI ASST 4 cut(s) 100, 174, 229, 250
SfaNI GCATC 2 cut(s) 217, 434
SmiMI CAYNNNNRTG 1 cut(s) 434
Sse9I AATT 3 cut(s) 17, 322, 377
SspMI CTAG 1 cut(s) 212
SstI GAGCTC 1 cut(s) 100
TaaI ACNGT 1 cut(s) 255
TaqI TCGA 1 cut(s) 442
TaqII GACCGA 1 cut(s) 11
TasI AATT 3 cut(s) 17, 322, 377
TspDTI ATGAA 4 cut(s) 21, 172, 257, 382
XapI RAATTY 1 cut(s) 322
XmiI GTMKAC 1 cut(s) 223
XspI CTAG 1 cut(s) 212
Zsp2I ATGCAT 2 cut(s) 6, 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.