Rroxscaffold_4G00323440

Involved in protein precursor import into chloroplasts. May be part of an intermediate translocation complex acting as a protein-conducting channel at the inner envelope

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0011870
Physical Location & Seq
Reverse (-)
54172561 .. 54194706
22146 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00323440.1

Sequence Viewer

Length: 930 bp
ATGAAAGAAGGAGAAGAAGGAACCGAGAAGAAAGTATCAGCAACAACCGGTTTTATTACGGGACAGCTCATGATGTTCATATCAATCTATTATGCGCCTCTGCATCTAGCATTGGAGAAAACCACAGAAGAAAGTGAGAAAAAAACAGAAGAGGATAAAAAAGAGGAAGCCAAAAGAAAGGAGAAAGTACGTATAGAAATAGTGGAAGCCTGGGATACTCAAAGGAGTTCAGGTTGTGGTTTTAGTGCTGAGGTGGCTTTAGATATTTTGTTTCTGAATTCCTTGAAGCTAGAAAGTAAAACCGACGGTCTGATCTCCGGAAATCATAAACCGAAGATAAAACATAGGGAAAGCTTTTTTGAAGCAGCTGAAAGTGTTCCTGGGAATAAAACTTCTGGATTCGCCTTACATTTCAAAAGCGATGTACTTAATTTGCTTGCTAAGATTGAACTTCTTCACCGATTTCTATCAAATCCAGTCGATTCACTAGCTAGAAGATCTACGAACCCAGACGTTCATCTAGAAAGAAATTTGATGAAACACAAAGGAAAAGGGTGGAGATCATGTGCAGTGACCACGCCGGGTAGAGATGGTAGTGACTTGACAGCTACAACCATTGGTAAAGCACGTTGGCAGGAACTTGACCATGTTGTGGAAGCACCGGAGAAAATTGTTGTTGTCAATCTTCAGACCGTACCGATTAATAATATCTCTCATTGGAAATGTGCAGAAATCATCACTAATATTAGAGAAGGTGTTGTACCGTGTTTTGCCAAGCGACGGCAATGTCGCCGATGTCGTTGGTGGCGTCGCCATTGCTATTTGCGATGGCGTAAATACCGTCGCCAAAGTTCATGCGACGGCCAAATTGCCGTCGCAAATAGCAATGGCGACGCCATCAACGACATCGGCGGCATTGTCGTCGTCTAG

Protein Analysis

309

Amino Acids

35.0

Weight (kDa)

9.19

Isoelectric Point (pI)

55.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ycf1 PF05758 1 - 40 6.1e-20 Ycf1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 786
AccB7I CCANNNNNTGG 1 cut(s) 652
AccIII TCCGGA 1 cut(s) 317
AciI CCGC 1 cut(s) 912
AcoI YGGCCR 1 cut(s) 862
AcsI RAATTY 2 cut(s) 277, 529
AcuI CTGAAG 1 cut(s) 671
AcyI GRCGYC 2 cut(s) 808, 894
AfaI GTAC 4 cut(s) 189, 426, 696, 762
AfiI CCNNNNNNNGG 2 cut(s) 652, 780
AgeI ACCGGT 1 cut(s) 47
AgsI TTSAA 4 cut(s) 286, 362, 415, 449
AjnI CCWGG 2 cut(s) 209, 379
AluBI AGCT 6 cut(s) 67, 289, 354, 368, 491, 608
AluI AGCT 6 cut(s) 67, 289, 354, 368, 491, 608
Aor13HI TCCGGA 1 cut(s) 317
AoxI GGCC 1 cut(s) 862
ApeKI GCWGC 1 cut(s) 365
ApoI RAATTY 2 cut(s) 277, 529
AseI ATTAAT 1 cut(s) 702
AsiGI ACCGGT 1 cut(s) 47
Asp700I GAANNNNTTC 2 cut(s) 375, 453
AspLEI GCGC 1 cut(s) 97
AsuC2I CCSGG 1 cut(s) 582
AsuHPI GGTGA 1 cut(s) 449
BarI GAAGNNNNNNTAC 2 cut(s) 744, 776
BbvCI CCTCAGC 1 cut(s) 249
BbvI GCAGC 1 cut(s) 377
BccI CCATC 3 cut(s) 584, 822, 905
BceAI ACGGC 3 cut(s) 797, 857, 877
BcgI CGANNNNNNTGC 1 cut(s) 904
BciT130I CCWGG 2 cut(s) 211, 381
BciVI GTATCC 1 cut(s) 208
BcnI CCSGG 1 cut(s) 582
BfaI CTAG 6 cut(s) 107, 290, 488, 492, 521, 928
BfuI GTATCC 1 cut(s) 208
BglII AGATCT 1 cut(s) 497
BisI GCNGC 2 cut(s) 366, 913
BlsI GCNGC 2 cut(s) 367, 914
Bme1390I CCNGG 3 cut(s) 211, 381, 582
BmiI GGNNCC 1 cut(s) 22
BmrFI CCNGG 3 cut(s) 211, 381, 582
BmsI GCATC 1 cut(s) 112
Bpu10I CCTNAGC 1 cut(s) 249
BpuMI CCSGG 1 cut(s) 582
BsaAI YACGTR 1 cut(s) 191
BsaBI GATNNNNATC 1 cut(s) 466
BsaHI GRCGYC 2 cut(s) 808, 894
BsaJI CCNNGG 2 cut(s) 210, 380
BsaWI WCCGGW 3 cut(s) 47, 317, 661
BsaXI ACNNNNNCTCC 4 cut(s) 217, 247, 550, 580
Bsc4I CCNNNNNNNGG 2 cut(s) 652, 780
Bse118I RCCGGY 1 cut(s) 47
Bse1I ACTGG 1 cut(s) 476
Bse3DI GCAATG 3 cut(s) 791, 814, 892
Bse8I GATNNNNATC 1 cut(s) 466
BseAI TCCGGA 1 cut(s) 317
BseBI CCWGG 2 cut(s) 211, 381
BseDI CCNNGG 2 cut(s) 210, 380
BseJI GATNNNNATC 1 cut(s) 466
BseLI CCNNNNNNNGG 2 cut(s) 652, 780
BseMI GCAATG 3 cut(s) 791, 814, 892
BseMII CTCAG 1 cut(s) 240
BseNI ACTGG 1 cut(s) 476
BseXI GCAGC 1 cut(s) 377
BsgI GTGCAG 2 cut(s) 588, 747
BshFI GGCC 1 cut(s) 864
BshTI ACCGGT 1 cut(s) 47
BsiSI CCGG 4 cut(s) 48, 318, 581, 662
BslFI GGGAC 1 cut(s) 75
BslI CCNNNNNNNGG 2 cut(s) 652, 780
BsmFI GGGAC 1 cut(s) 75
BsnI GGCC 1 cut(s) 864
Bsp13I TCCGGA 1 cut(s) 317
Bsp143I GATC 3 cut(s) 312, 497, 560
BspACI CCGC 1 cut(s) 912
BspANI GGCC 1 cut(s) 864
BspCNI CTCAG 1 cut(s) 241
BspEI TCCGGA 1 cut(s) 317
BspHI TCATGA 1 cut(s) 69
BspLI GGNNCC 1 cut(s) 22
BsrDI GCAATG 3 cut(s) 791, 814, 892
BsrFI RCCGGY 1 cut(s) 47
BsrI ACTGG 1 cut(s) 476
BssAI RCCGGY 1 cut(s) 47
BssECI CCNNGG 2 cut(s) 210, 380
BssMI GATC 3 cut(s) 312, 497, 560
BssNI GRCGYC 2 cut(s) 808, 894
Bst2UI CCWGG 2 cut(s) 211, 381
Bst4CI ACNGT 4 cut(s) 308, 694, 765, 842
Bst6I CTCTTC 1 cut(s) 144
BstACI GRCGYC 2 cut(s) 808, 894
BstBAI YACGTR 1 cut(s) 191
BstC8I GCNNGC 1 cut(s) 438
BstDEI CTNAG 2 cut(s) 249, 441
BstHHI GCGC 1 cut(s) 97
BstKTI GATC 3 cut(s) 315, 500, 563
BstMBI GATC 3 cut(s) 312, 497, 560
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstNI CCWGG 2 cut(s) 211, 381
BstSCI CCNGG 3 cut(s) 209, 379, 580
BstSNI TACGTA 1 cut(s) 191
BstV1I GCAGC 1 cut(s) 377
BstX2I RGATCY 1 cut(s) 497
BstYI RGATCY 1 cut(s) 497
BsuI GTATCC 1 cut(s) 208
BsuRI GGCC 1 cut(s) 864
BtgZI GCGATG 2 cut(s) 435, 841
BtsI GCAGTG 1 cut(s) 576
BtsIMutI CAGTG 1 cut(s) 576
Cac8I GCNNGC 1 cut(s) 438
CciI TCATGA 1 cut(s) 69
CfoI GCGC 1 cut(s) 97
Cfr10I RCCGGY 1 cut(s) 47
CseI GACGC 2 cut(s) 797, 902
Csp6I GTAC 4 cut(s) 188, 425, 695, 761
CspAI ACCGGT 1 cut(s) 47
CviAII CATG 4 cut(s) 70, 564, 647, 855
CviQI GTAC 4 cut(s) 188, 425, 695, 761
DdeI CTNAG 2 cut(s) 249, 441
DpnI GATC 3 cut(s) 314, 499, 562
DpnII GATC 3 cut(s) 312, 497, 560
DrdI GACNNNNNNGTC 1 cut(s) 786
DseDI GACNNNNNNGTC 1 cut(s) 786
EaeI YGGCCR 1 cut(s) 862
Eam1104I CTCTTC 1 cut(s) 144
EarI CTCTTC 1 cut(s) 144
Eco105I TACGTA 1 cut(s) 191
Eco57I CTGAAG 1 cut(s) 671
EcoRI GAATTC 1 cut(s) 277
EcoRII CCWGG 2 cut(s) 209, 379
FaeI CATG 4 cut(s) 73, 567, 650, 858
FaiI YATR 9 cut(s) 71, 80, 93, 194, 327, 345, 565, 648, 856
FaqI GGGAC 1 cut(s) 75
FatI CATG 4 cut(s) 69, 563, 646, 854
Fnu4HI GCNGC 2 cut(s) 366, 913
Fsp4HI GCNGC 2 cut(s) 366, 913
FspBI CTAG 6 cut(s) 107, 290, 488, 492, 521, 928
GlaI GCGC 1 cut(s) 96
GluI GCNGC 2 cut(s) 366, 913
HaeIII GGCC 1 cut(s) 864
HapII CCGG 4 cut(s) 48, 318, 581, 662
HgaI GACGC 2 cut(s) 797, 902
HhaI GCGC 1 cut(s) 97
Hin1I GRCGYC 2 cut(s) 808, 894
Hin1II CATG 4 cut(s) 73, 567, 650, 858
Hin6I GCGC 1 cut(s) 95
HinP1I GCGC 1 cut(s) 95
HindIII AAGCTT 1 cut(s) 352
HinfI GANTC 2 cut(s) 399, 482
HpaII CCGG 4 cut(s) 48, 318, 581, 662
HphI GGTGA 1 cut(s) 449
Hpy188I TCNGA 3 cut(s) 276, 312, 690
Hpy188III TCNNGA 4 cut(s) 70, 318, 396, 521
Hpy99I CGWCG 8 cut(s) 308, 783, 813, 846, 863, 878, 896, 926
HpyAV CCTTC 2 cut(s) 11, 746
HpyCH4III ACNGT 4 cut(s) 308, 694, 765, 842
HpyCH4IV ACGT 3 cut(s) 190, 513, 628
HpyCH4V TGCA 3 cut(s) 103, 569, 728
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpyF3I CTNAG 2 cut(s) 249, 441
HpySE526I ACGT 3 cut(s) 190, 513, 628
Hsp92I GRCGYC 2 cut(s) 808, 894
Hsp92II CATG 4 cut(s) 73, 567, 650, 858
HspAI GCGC 1 cut(s) 95
Kpn2I TCCGGA 1 cut(s) 317
Kzo9I GATC 3 cut(s) 312, 497, 560
Lsp1109I GCAGC 1 cut(s) 377
LweI GCATC 1 cut(s) 112
MaeI CTAG 6 cut(s) 107, 290, 488, 492, 521, 928
MaeII ACGT 3 cut(s) 190, 513, 628
MaeIII GTNAC 2 cut(s) 571, 596
MalI GATC 3 cut(s) 314, 499, 562
MboI GATC 3 cut(s) 312, 497, 560
MboII GAAGA 8 cut(s) 26, 40, 140, 161, 346, 446, 507, 677
MflI RGATCY 1 cut(s) 497
MluCI AATT 5 cut(s) 277, 430, 529, 669, 867
MnlI CCTC 4 cut(s) 108, 145, 157, 244
MroI TCCGGA 1 cut(s) 317
MroXI GAANNNNTTC 2 cut(s) 375, 453
MseI TTAA 2 cut(s) 429, 702
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 4 cut(s) 48, 318, 581, 662
MspR9I CCNGG 3 cut(s) 211, 381, 582
MvaI CCWGG 2 cut(s) 211, 381
MwoI GCNNNNNNNGC 1 cut(s) 254
NciI CCSGG 1 cut(s) 582
NdeII GATC 3 cut(s) 312, 497, 560
NlaIII CATG 4 cut(s) 73, 567, 650, 858
NlaIV GGNNCC 1 cut(s) 22
NmuCI GTSAC 2 cut(s) 571, 596
PagI TCATGA 1 cut(s) 69
PcsI WCGNNNNNNNCGW 3 cut(s) 796, 805, 900
PdmI GAANNNNTTC 2 cut(s) 375, 453
PfeI GAWTC 2 cut(s) 399, 482
PflMI CCANNNNNTGG 1 cut(s) 652
PinAI ACCGGT 1 cut(s) 47
PkrI GCNGC 2 cut(s) 367, 914
Ppu21I YACGTR 1 cut(s) 191
PshBI ATTAAT 1 cut(s) 702
Psp6I CCWGG 2 cut(s) 209, 379
PspGI CCWGG 2 cut(s) 209, 379
PspN4I GGNNCC 1 cut(s) 22
PsuI RGATCY 1 cut(s) 497
PvuII CAGCTG 1 cut(s) 368
RsaI GTAC 4 cut(s) 189, 426, 696, 762
RsaNI GTAC 4 cut(s) 188, 425, 695, 761
SaqAI TTAA 2 cut(s) 429, 702
SatI GCNGC 2 cut(s) 366, 913
Sau3AI GATC 3 cut(s) 312, 497, 560
ScrFI CCNGG 3 cut(s) 211, 381, 582
SfaNI GCATC 1 cut(s) 112
SnaBI TACGTA 1 cut(s) 191
Sse9I AATT 5 cut(s) 277, 430, 529, 669, 867
SsiI CCGC 1 cut(s) 912
SspI AATATT 1 cut(s) 745
SspMI CTAG 6 cut(s) 107, 290, 488, 492, 521, 928
StyD4I CCNGG 3 cut(s) 209, 379, 580
TaaI ACNGT 4 cut(s) 308, 694, 765, 842
TaiI ACGT 3 cut(s) 193, 516, 631
TaqI TCGA 1 cut(s) 480
TasI AATT 5 cut(s) 277, 430, 529, 669, 867
TatI WGTACW 1 cut(s) 424
TauI GCSGC 1 cut(s) 915
TfiI GAWTC 2 cut(s) 399, 482
Tru1I TTAA 2 cut(s) 429, 702
Tru9I TTAA 2 cut(s) 429, 702
TscAI CASTG 1 cut(s) 576
TseFI GTSAC 2 cut(s) 571, 596
TseI GCWGC 1 cut(s) 365
Tsp45I GTSAC 2 cut(s) 571, 596
TspDTI ATGAA 5 cut(s) 17, 67, 506, 551, 843
TspRI CASTG 1 cut(s) 576
Van91I CCANNNNNTGG 1 cut(s) 652
VspI ATTAAT 1 cut(s) 702
XapI RAATTY 2 cut(s) 277, 529
XbaI TCTAGA 1 cut(s) 520
XmnI GAANNNNTTC 2 cut(s) 375, 453
XspI CTAG 6 cut(s) 107, 290, 488, 492, 521, 928
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.