FvH4_1g01971

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0009592
Physical Location & Seq
Reverse (-)
1035640 .. 1039995
4356 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g01971.t2

Sequence Viewer

Length: 1344 bp
ATGGTGGGAAACACTTCACACTCCCACAGTGACCATACCCCTCAACCGGCGATGGCTGACATCAGCGGCGGCGGAGCAGCCAAGAAAACCGCCAAGCGCGGCGGCAAGCCGAAGAACGACAAGAACTGCGACCTCGATAAACGAAAACTCATCGGCCTTGTGACCTCCGCCACCTCACTCGCCCACTCCTTCCTCTCCCGAAACGACCTCCTCCTCCTCCCTTCCCAAACCCTAACCCTAGAAACCCTCCTCTCCTCCGCCTCCTCCGCCCTCTCCCCTCTCTCATCTCCTCCTCCGCCGCCGCCGCCGCCTCAACCGCAACCGCAACAGCCGACTCAAGAATGCTGGTTCCGCCGCTTCCTCTCCGACACCGCCGCCGGCGCCGACTCGCGGTGGCGCCACGCCTTCCGCATGTCCGAGCACTCCTTCTCGATCCTTCTCTCCATCCTCTCCCCGTTCCTGACCGCCGCAATCCCTTCAATTCCGCCGAACTACGCTCTCGCCGCCGCCGTTTACCGCCTCGCCCACGGCGCCGGCTACAAGGCCGTCGGCCGCCGCTTCGGGATGGACTCTCCGGCGGCCTGCCGGGCGTTCTACGCCGTCTGCAAGTCGGTGAATGATAAATTGGGGAACTTGTTCGAGTTCCGGTCCGATATGGCCCGGATTGTCGCCGCATTCGGGTGGATTTCGCTGCCGAATTGCTGCGGCGTGTTAGGGTTTGGCAGATTTGGTATCGATAGTGCAATGTTGGGGCCGAATGCGGCGCTGCTGGTTCAGGCATTGGTGGATTCCGAGGGGAGGTTTCTGGATGTGTCGGCTGGCTGGCCGAGCACAATGAAACCGGAAGCGATTTTCCGGCAGAGTAAGCTGTATTTGGGTGTGGAGGAGACCAGGGAGTTGCTGAATGGTCCTGCTCATGAGCTCAGTGATGGCAATGCGCTTCCTCAGTACATATTGGGGGAGTCTTGCTTTCCTCTGTTGCGTTGGCTTTTGACACCTTATGTGAGATCAAATGTGGAAGATGAGAGTTTTGGTGTGTCGGAGAAGGCGTTTAACTCGGCGCATAGTCGTGCAATGGGGCTGGTTGGGACGGCATTTGCGAGGGTAAGGGCTAGGTGGCAGCTGCTGAGTAGGGAGTGGAAGGAGGAGTGTAGGGAGTTTTTGCCGTTTGTGGTGGTTACTGGTTGTCTGCTGAATAATTTTCTGATGAAGTGTAGTGAGCCGCAGCCGGATGTCAATGAGGAGTGCTTGGAGGAGCTTCCGGTGTTTGAAGGAGAAATGGATGCGAGTGGCGAGAGGATTAGGGATGCGCTTGCCACGCACTTGAGCCGGATTGGTAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

448

Amino Acids

48.81

Weight (kDa)

7.57

Isoelectric Point (pI)

56.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 239 - 383 1.8e-14 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 380, 396, 530
AccII CGCG 2 cut(s) 99, 391
AclWI GGATC 1 cut(s) 427
AcoI YGGCCR 2 cut(s) 550, 824
AcyI GRCGYC 3 cut(s) 381, 397, 531
AfaI GTAC 1 cut(s) 950
AfiI CCNNNNNNNGG 4 cut(s) 46, 390, 678, 798
AgsI TTSAA 2 cut(s) 480, 1271
AjnI CCWGG 1 cut(s) 890
AjuI GAANNNNNNNTTGG 2 cut(s) 608, 640
AluBI AGCT 4 cut(s) 868, 922, 1123, 1258
AluI AGCT 4 cut(s) 868, 922, 1123, 1258
Alw21I GWGCWC 3 cut(s) 423, 833, 924
Alw26I GTCTC 1 cut(s) 881
AlwI GGATC 1 cut(s) 427
AlwNI CAGNNNCTG 1 cut(s) 1126
AoxI GGCC 7 cut(s) 154, 543, 550, 579, 657, 752, 824
ApeKI GCWGC 7 cut(s) 77, 691, 702, 766, 1120, 1123, 1225
Asp700I GAANNNNTTC 2 cut(s) 13, 635
AspLEI GCGC 8 cut(s) 99, 383, 399, 533, 766, 940, 1063, 1312
AspS9I GGNCC 4 cut(s) 648, 658, 752, 908
AsuC2I CCSGG 2 cut(s) 587, 661
AsuHPI GGTGA 1 cut(s) 625
AvaII GGWCC 2 cut(s) 648, 908
BanI GGYRCC 3 cut(s) 380, 396, 530
BanII GRGCYC 1 cut(s) 924
Bbv12I GWGCWC 3 cut(s) 423, 833, 924
BbvI GCAGC 7 cut(s) 89, 678, 689, 753, 1110, 1132, 1237
BccI CCATC 4 cut(s) 46, 452, 559, 923
BceAI ACGGC 6 cut(s) 494, 530, 544, 584, 1107, 1150
BciT130I CCWGG 1 cut(s) 892
BcnI CCSGG 2 cut(s) 587, 661
BcoDI GTCTC 1 cut(s) 881
BfaI CTAG 2 cut(s) 239, 1113
BfoI RGCGCY 4 cut(s) 384, 400, 534, 767
BglI GCCNNNNNGGC 1 cut(s) 587
Bme1390I CCNGG 3 cut(s) 587, 661, 892
Bme18I GGWCC 2 cut(s) 648, 908
BmgT120I GGNCC 4 cut(s) 648, 658, 752, 908
BmiI GGNNCC 5 cut(s) 350, 382, 398, 532, 753
BmrFI CCNGG 3 cut(s) 587, 661, 892
BmsI GCATC 2 cut(s) 1273, 1297
BpuEI CTTGAG 1 cut(s) 321
BpuMI CCSGG 2 cut(s) 587, 661
Bsa29I ATCGAT 1 cut(s) 735
BsaHI GRCGYC 3 cut(s) 381, 397, 531
BsaI GGTCTC 1 cut(s) 881
BsaJI CCNNGG 3 cut(s) 526, 792, 891
BsaWI WCCGGW 3 cut(s) 645, 841, 1261
BsaXI ACNNNNNCTCC 2 cut(s) 1034, 1064
Bsc4I CCNNNNNNNGG 4 cut(s) 46, 390, 678, 798
Bse118I RCCGGY 3 cut(s) 46, 377, 533
Bse1I ACTGG 1 cut(s) 1186
Bse3DI GCAATG 3 cut(s) 750, 940, 1080
BseBI CCWGG 1 cut(s) 892
BseCI ATCGAT 1 cut(s) 735
BseDI CCNNGG 3 cut(s) 526, 792, 891
BseGI GGATG 6 cut(s) 444, 570, 814, 1237, 1288, 1312
BseLI CCNNNNNNNGG 4 cut(s) 46, 390, 678, 798
BseMI GCAATG 3 cut(s) 750, 940, 1080
BseMII CTCAG 3 cut(s) 937, 959, 1118
BseNI ACTGG 1 cut(s) 1186
BseX3I CGGCCG 1 cut(s) 550
BseXI GCAGC 7 cut(s) 89, 678, 689, 753, 1110, 1132, 1237
Bsh1236I CGCG 2 cut(s) 99, 391
Bsh1285I CGRYCG 1 cut(s) 553
BshFI GGCC 7 cut(s) 156, 545, 552, 581, 659, 754, 826
BshNI GGYRCC 3 cut(s) 380, 396, 530
BshVI ATCGAT 1 cut(s) 735
BsiEI CGRYCG 1 cut(s) 553
BsiHKAI GWGCWC 3 cut(s) 423, 833, 924
BslFI GGGAC 1 cut(s) 1102
BslI CCNNNNNNNGG 4 cut(s) 46, 390, 678, 798
BsmAI GTCTC 1 cut(s) 881
BsmFI GGGAC 1 cut(s) 1102
BsmI GAATGC 3 cut(s) 347, 674, 763
BsnI GGCC 7 cut(s) 156, 545, 552, 581, 659, 754, 826
Bso31I GGTCTC 1 cut(s) 881
Bsp1286I GDGCHC 3 cut(s) 423, 833, 924
Bsp143I GATC 2 cut(s) 432, 1007
BspANI GGCC 7 cut(s) 156, 545, 552, 581, 659, 754, 826
BspCNI CTCAG 3 cut(s) 936, 958, 1119
BspDI ATCGAT 1 cut(s) 735
BspFNI CGCG 2 cut(s) 99, 391
BspHI TCATGA 1 cut(s) 916
BspLI GGNNCC 5 cut(s) 350, 382, 398, 532, 753
BspPI GGATC 1 cut(s) 427
BspT107I GGYRCC 3 cut(s) 380, 396, 530
BspTNI GGTCTC 1 cut(s) 881
BsrDI GCAATG 3 cut(s) 750, 940, 1080
BsrFI RCCGGY 3 cut(s) 46, 377, 533
BsrI ACTGG 1 cut(s) 1186
BssAI RCCGGY 3 cut(s) 46, 377, 533
BssECI CCNNGG 3 cut(s) 526, 792, 891
BssMI GATC 2 cut(s) 432, 1007
BssNI GRCGYC 3 cut(s) 381, 397, 531
Bst2UI CCWGG 1 cut(s) 892
Bst4CI ACNGT 1 cut(s) 29
BstACI GRCGYC 3 cut(s) 381, 397, 531
BstC8I GCNNGC 7 cut(s) 107, 379, 535, 583, 820, 824, 1314
BstDEI CTNAG 3 cut(s) 923, 945, 1127
BstDSI CCRYGG 1 cut(s) 526
BstF5I GGATG 6 cut(s) 444, 570, 814, 1237, 1288, 1312
BstFNI CGCG 2 cut(s) 99, 391
BstH2I RGCGCY 4 cut(s) 384, 400, 534, 767
BstHHI GCGC 8 cut(s) 99, 383, 399, 533, 766, 940, 1063, 1312
BstKTI GATC 2 cut(s) 435, 1010
BstMAI GTCTC 1 cut(s) 881
BstMBI GATC 2 cut(s) 432, 1007
BstMCI CGRYCG 1 cut(s) 553
BstNI CCWGG 1 cut(s) 892
BstNSI RCATGY 1 cut(s) 415
BstSCI CCNGG 3 cut(s) 585, 659, 890
BstUI CGCG 2 cut(s) 99, 391
BstV1I GCAGC 7 cut(s) 89, 678, 689, 753, 1110, 1132, 1237
BstZI CGGCCG 1 cut(s) 550
Bsu15I ATCGAT 1 cut(s) 735
BsuRI GGCC 7 cut(s) 156, 545, 552, 581, 659, 754, 826
BsuTUI ATCGAT 1 cut(s) 735
BtgI CCRYGG 1 cut(s) 526
BtgZI GCGATG 1 cut(s) 65
BtsCI GGATG 6 cut(s) 444, 570, 814, 1237, 1288, 1312
BtsIMutI CAGTG 2 cut(s) 34, 931
Cac8I GCNNGC 7 cut(s) 107, 379, 535, 583, 820, 824, 1314
CaiI CAGNNNCTG 1 cut(s) 1126
CciI TCATGA 1 cut(s) 916
CfoI GCGC 8 cut(s) 99, 383, 399, 533, 766, 940, 1063, 1312
Cfr10I RCCGGY 3 cut(s) 46, 377, 533
Cfr13I GGNCC 4 cut(s) 648, 658, 752, 908
ClaI ATCGAT 1 cut(s) 735
CpoI CGGWCCG 1 cut(s) 648
Csp6I GTAC 1 cut(s) 949
CspI CGGWCCG 1 cut(s) 648
CviAII CATG 2 cut(s) 412, 917
CviQI GTAC 1 cut(s) 949
DdeI CTNAG 3 cut(s) 923, 945, 1127
DinI GGCGCC 3 cut(s) 382, 398, 532
DpnI GATC 2 cut(s) 434, 1009
DpnII GATC 2 cut(s) 432, 1007
EaeI YGGCCR 2 cut(s) 550, 824
EagI CGGCCG 1 cut(s) 550
EciI GGCGGA 7 cut(s) 87, 157, 247, 256, 285, 341, 474
Ecl136II GAGCTC 1 cut(s) 922
EclXI CGGCCG 1 cut(s) 550
Eco24I GRGCYC 1 cut(s) 924
Eco31I GGTCTC 1 cut(s) 881
Eco47I GGWCC 2 cut(s) 648, 908
Eco52I CGGCCG 1 cut(s) 550
Eco53kI GAGCTC 1 cut(s) 922
EcoICRI GAGCTC 1 cut(s) 922
EcoRII CCWGG 1 cut(s) 890
EcoT38I GRGCYC 1 cut(s) 924
EgeI GGCGCC 3 cut(s) 382, 398, 532
EheI GGCGCC 3 cut(s) 382, 398, 532
FaeI CATG 2 cut(s) 415, 920
FaiI YATR 7 cut(s) 36, 413, 656, 918, 953, 1002, 1065
FaqI GGGAC 1 cut(s) 1102
FatI CATG 2 cut(s) 411, 916
FokI GGATG 6 cut(s) 431, 577, 821, 1244, 1295, 1319
FriOI GRGCYC 1 cut(s) 924
FspBI CTAG 2 cut(s) 239, 1113
GlaI GCGC 8 cut(s) 98, 382, 398, 532, 765, 939, 1062, 1311
HaeII RGCGCY 4 cut(s) 384, 400, 534, 767
HaeIII GGCC 7 cut(s) 156, 545, 552, 581, 659, 754, 826
HhaI GCGC 8 cut(s) 99, 383, 399, 533, 766, 940, 1063, 1312
Hin1I GRCGYC 3 cut(s) 381, 397, 531
Hin1II CATG 2 cut(s) 415, 920
Hin6I GCGC 8 cut(s) 97, 381, 397, 531, 764, 938, 1061, 1310
HinP1I GCGC 8 cut(s) 97, 381, 397, 531, 764, 938, 1061, 1310
HinfI GANTC 5 cut(s) 334, 386, 569, 788, 962
HphI GGTGA 1 cut(s) 625
Hpy166II GTNNAC 1 cut(s) 514
Hpy188I TCNGA 6 cut(s) 367, 418, 652, 793, 1042, 1206
Hpy188III TCNNGA 7 cut(s) 198, 338, 430, 460, 562, 806, 917
Hpy8I GTNNAC 1 cut(s) 514
Hpy99I CGWCG 1 cut(s) 551
HpyAV CCTTC 9 cut(s) 199, 231, 415, 436, 446, 486, 1039, 1135, 1265
HpyCH4III ACNGT 1 cut(s) 29
HpyCH4V TGCA 3 cut(s) 606, 743, 1073
HpyF3I CTNAG 3 cut(s) 923, 945, 1127
Hsp92I GRCGYC 3 cut(s) 381, 397, 531
Hsp92II CATG 2 cut(s) 415, 920
HspAI GCGC 8 cut(s) 97, 381, 397, 531, 764, 938, 1061, 1310
KasI GGCGCC 3 cut(s) 380, 396, 530
KroI GCCGGC 2 cut(s) 377, 533
KroNI GCCGGC 2 cut(s) 379, 535
Kzo9I GATC 2 cut(s) 432, 1007
LmnI GCTCC 2 cut(s) 74, 1255
Lsp1109I GCAGC 7 cut(s) 89, 678, 689, 753, 1110, 1132, 1237
LweI GCATC 2 cut(s) 1273, 1297
MaeI CTAG 2 cut(s) 239, 1113
MaeIII GTNAC 3 cut(s) 29, 160, 1177
MalI GATC 2 cut(s) 434, 1009
MboI GATC 2 cut(s) 432, 1007
MboII GAAGA 2 cut(s) 124, 1031
MhlI GDGCHC 3 cut(s) 423, 833, 924
MluCI AATT 4 cut(s) 480, 623, 697, 1198
Mly113I GGCGCC 3 cut(s) 381, 397, 531
MlyI GAGTC 4 cut(s) 328, 380, 563, 971
MmeI TCCRAC 2 cut(s) 390, 1020
MreI CGCCGGCG 1 cut(s) 377
MroNI GCCGGC 2 cut(s) 377, 533
MroXI GAANNNNTTC 2 cut(s) 13, 635
MseI TTAA 1 cut(s) 1053
MslI CAYNNNNRTG 2 cut(s) 679, 1068
MspA1I CMGCKG 2 cut(s) 66, 1123
MspR9I CCNGG 3 cut(s) 587, 661, 892
Mva1269I GAATGC 3 cut(s) 347, 674, 763
MvaI CCWGG 1 cut(s) 892
MvnI CGCG 2 cut(s) 99, 391
NaeI GCCGGC 2 cut(s) 379, 535
NarI GGCGCC 3 cut(s) 381, 397, 531
NciI CCSGG 2 cut(s) 587, 661
NdeII GATC 2 cut(s) 432, 1007
NgoMIV GCCGGC 2 cut(s) 377, 533
NlaIII CATG 2 cut(s) 415, 920
NlaIV GGNNCC 5 cut(s) 350, 382, 398, 532, 753
NmeAIII GCCGAG 2 cut(s) 852, 1037
NmuCI GTSAC 2 cut(s) 29, 160
NspI RCATGY 1 cut(s) 415
PagI TCATGA 1 cut(s) 916
PcsI WCGNNNNNNNCGW 3 cut(s) 507, 1046, 1097
PctI GAATGC 3 cut(s) 347, 674, 763
PdiI GCCGGC 2 cut(s) 379, 535
PdmI GAANNNNTTC 2 cut(s) 13, 635
PfeI GAWTC 1 cut(s) 788
PleI GAGTC 4 cut(s) 328, 380, 563, 970
PluTI GGCGCC 3 cut(s) 384, 400, 534
PpsI GAGTC 4 cut(s) 328, 380, 563, 970
Psp124BI GAGCTC 1 cut(s) 924
Psp6I CCWGG 1 cut(s) 890
PspGI CCWGG 1 cut(s) 890
PspN4I GGNNCC 5 cut(s) 350, 382, 398, 532, 753
PspPI GGNCC 4 cut(s) 648, 658, 752, 908
PstNI CAGNNNCTG 1 cut(s) 1126
PvuII CAGCTG 1 cut(s) 1123
RsaI GTAC 1 cut(s) 950
RsaNI GTAC 1 cut(s) 949
RseI CAYNNNNRTG 2 cut(s) 679, 1068
Rsr2I CGGWCCG 1 cut(s) 648
RsrII CGGWCCG 1 cut(s) 648
SacI GAGCTC 1 cut(s) 924
SaqAI TTAA 1 cut(s) 1053
Sau3AI GATC 2 cut(s) 432, 1007
Sau96I GGNCC 4 cut(s) 648, 658, 752, 908
SchI GAGTC 4 cut(s) 328, 380, 563, 971
ScrFI CCNGG 3 cut(s) 587, 661, 892
SduI GDGCHC 3 cut(s) 423, 833, 924
SfaNI GCATC 2 cut(s) 1273, 1297
SfoI GGCGCC 3 cut(s) 382, 398, 532
SgrAI CRCCGGYG 1 cut(s) 377
SinI GGWCC 2 cut(s) 648, 908
SmiMI CAYNNNNRTG 2 cut(s) 679, 1068
SmlI CTYRAG 2 cut(s) 336, 1324
SmoI CTYRAG 2 cut(s) 336, 1324
Sse9I AATT 4 cut(s) 480, 623, 697, 1198
SspDI GGCGCC 3 cut(s) 380, 396, 530
SspMI CTAG 2 cut(s) 239, 1113
SstI GAGCTC 1 cut(s) 924
StyD4I CCNGG 3 cut(s) 585, 659, 890
TaaI ACNGT 1 cut(s) 29
TaqI TCGA 4 cut(s) 135, 431, 639, 735
TasI AATT 4 cut(s) 480, 623, 697, 1198
TatI WGTACW 1 cut(s) 948
TfiI GAWTC 1 cut(s) 788
Tru1I TTAA 1 cut(s) 1053
Tru9I TTAA 1 cut(s) 1053
TscAI CASTG 2 cut(s) 34, 931
TseFI GTSAC 2 cut(s) 29, 160
TseI GCWGC 7 cut(s) 77, 691, 702, 766, 1120, 1123, 1225
Tsp45I GTSAC 2 cut(s) 29, 160
TspDTI ATGAA 2 cut(s) 851, 1223
TspRI CASTG 2 cut(s) 34, 931
VpaK11BI GGWCC 2 cut(s) 648, 908
XceI RCATGY 1 cut(s) 415
XmnI GAANNNNTTC 2 cut(s) 13, 635
XspI CTAG 2 cut(s) 239, 1113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.