pycom02g01620

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0009592
Physical Location & Seq
Reverse (-)
968037 .. 969365
1329 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g01620.1

Sequence Viewer

Length: 1329 bp
ATGGCTGCCGGCGGCGGAGTCGGCGGTACGGCCACAAGCACCACCAAAGGCAATGCCAGAAACAATAAACAAAGCCAAACCCACAGCAACAACAAAAAGCCCCTCAATCAACACCACCTCGTCAGCCTCGTAACCGCCGCCACGTCACTGGCTCACTCCTTCCTCTTCCAAAACGACCTCCTCCTCCTTCCTGCCCAAACCCTAACCCTTGAATCCCTCCTCTCCTCCGCCTCCACCTCCCTGTCCACCCTCCTCTGCTTCCCCAACCCACGACCGCAATCACCATTACGCCCACCGCCGCCGCCTTTAGAATGTTGGTTCAGCCGCTTCCTCTCCGCCACCGCCGCAGACGGAGACGGAGACTTCCGCTGGTCCCAAATCTTCCGCATGTCCGAACACTCCTTCTCCGTCCTCCTCTCCCTCCTCTCCCCATTTCTCAACTCCAGAATCCCTTCAATCCCACCCAATTTCGTCCTCGCCGCCGCAATTTACCGCCTGGCCCACGGCGCGAGCTACAAGGCGGTCGGAAGGCGCTTCGGGCTCAACTCCATGGATACCTGCCGCGCATTTTACGCCGTATGTAAGGCGGTGAACGATCAACTGGGGAACTTGTTCGAATTCCGGTCAGATATATCCCGGGTATTGGCGGCGTTCGGGTGGATTTCGCTGCCGAATTGCTGTGGGGTTTTAGGGTTTTCGAGATTTGAGGTTGGTGGTGAATTGCTGGGGGCAAATGGGTCATTGCTGGTTCAGGCAGTGGTGGATTCTGAGGGGAGGTTTCTCGATGTCTCCGCCGGGTGGCCATCCACCATGAAACCAGAATCGATTTTCCGGCAGAGTAAGCTTTATTTGGGTGTGGAGGAGTCCAGGGAATTGCTCAGTGGTCCTGCTTTTGAGCTTGGGAATGGGAATTCCATTCCTCAGTACATACTGGGGGACTCTTGCTTCCCTCTATTGCCATGGCTTCTAACACCTTATGTAAGGTCGGATGAGGCGGATAGTTTCGGTTCAATGGAGAAGGCGTTTAATGCGGTGCATTATCGGGCAATGGGGTTGGTTGGGACAGCTTTTGGGAGGGTTCGGGCGCGGTGGCAGCTTTTGGCTAGGCAGTGGAAGGAAGAGTGTGCGGAGTTTCTGCCGTTTGTGGTGGTTACAGGGTGTCTGCTGCATAACTTTCTGATCAAGTGTAGCGAGCCGATTTCAGATGACAATGTGAGGGGCTTAAAGGAGGAGGAGCTTCCTGTTTTTGATGGACAAGTGAACGAGAGTGGCGAGCGGATGAGGGATGTCCTCGCCATGCACTTGAGCCGGGTAAGCCTCAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

443

Amino Acids

48.5

Weight (kDa)

8.03

Isoelectric Point (pI)

49.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 247 - 391 7.4e-17 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 566
AccBSI CCGCTC 1 cut(s) 1276
AccII CGCG 3 cut(s) 509, 564, 1087
AcoI YGGCCR 2 cut(s) 30, 800
AcsI RAATTY 2 cut(s) 617, 910
AfaI GTAC 2 cut(s) 28, 926
AfiI CCNNNNNNNGG 2 cut(s) 643, 798
AgsI TTSAA 3 cut(s) 212, 456, 1011
AjiI CACGTC 1 cut(s) 144
AjnI CCWGG 2 cut(s) 495, 866
AluBI AGCT 6 cut(s) 513, 844, 898, 1067, 1096, 1237
AluI AGCT 6 cut(s) 513, 844, 898, 1067, 1096, 1237
Alw26I GTCTC 3 cut(s) 348, 354, 793
Ama87I CYCGRG 1 cut(s) 636
AoxI GGCC 3 cut(s) 30, 498, 800
ApeKI GCWGC 4 cut(s) 5, 667, 1093, 1165
ApoI RAATTY 2 cut(s) 617, 910
Asp700I GAANNNNTTC 2 cut(s) 451, 611
AspLEI GCGC 4 cut(s) 509, 534, 566, 1087
AspS9I GGNCC 3 cut(s) 372, 499, 884
AsuC2I CCSGG 4 cut(s) 637, 638, 796, 1310
AsuHPI GGTGA 3 cut(s) 273, 601, 728
AsuII TTCGAA 1 cut(s) 615
AvaI CYCGRG 1 cut(s) 636
AvaII GGWCC 2 cut(s) 372, 884
BalI TGGCCA 1 cut(s) 802
BanII GRGCYC 1 cut(s) 543
BbvI GCAGC 3 cut(s) 654, 1105, 1152
BccI CCATC 2 cut(s) 811, 1244
BceAI ACGGC 4 cut(s) 45, 520, 560, 1123
BciT130I CCWGG 2 cut(s) 497, 868
BciVI GTATCC 1 cut(s) 547
BclI TGATCA 1 cut(s) 1179
BcnI CCSGG 4 cut(s) 637, 638, 796, 1310
BcoDI GTCTC 3 cut(s) 348, 354, 793
BfaI CTAG 1 cut(s) 1104
BfoI RGCGCY 1 cut(s) 535
BfuAI ACCTGC 1 cut(s) 566
BfuI GTATCC 1 cut(s) 547
Bme1390I CCNGG 6 cut(s) 497, 637, 638, 796, 868, 1310
Bme18I GGWCC 2 cut(s) 372, 884
BmeT110I CYCGRG 1 cut(s) 636
BmgBI CACGTC 1 cut(s) 144
BmgT120I GGNCC 3 cut(s) 372, 499, 884
BmiI GGNNCC 1 cut(s) 374
BmrFI CCNGG 6 cut(s) 497, 637, 638, 796, 868, 1310
BmrI ACTGGG 2 cut(s) 611, 941
BmuI ACTGGG 2 cut(s) 611, 941
BpmI CTGGAG 1 cut(s) 427
Bpu14I TTCGAA 1 cut(s) 615
BpuEI CTTGAG 1 cut(s) 1324
BpuMI CCSGG 4 cut(s) 637, 638, 796, 1310
Bsa29I ATCGAT 1 cut(s) 824
BsaJI CCNNGG 5 cut(s) 502, 549, 636, 867, 959
BsaWI WCCGGW 1 cut(s) 621
BsaXI ACNNNNNCTCC 4 cut(s) 389, 419, 1007, 1037
Bsc4I CCNNNNNNNGG 2 cut(s) 643, 798
Bse118I RCCGGY 1 cut(s) 8
Bse1I ACTGG 3 cut(s) 153, 606, 936
Bse3DI GCAATG 3 cut(s) 58, 740, 1053
BseBI CCWGG 2 cut(s) 497, 868
BseCI ATCGAT 1 cut(s) 824
BseDI CCNNGG 5 cut(s) 502, 549, 636, 867, 959
BseGI GGATG 4 cut(s) 803, 994, 1284, 1291
BseLI CCNNNNNNNGG 2 cut(s) 643, 798
BseMI GCAATG 3 cut(s) 58, 740, 1053
BseMII CTCAG 3 cut(s) 759, 892, 935
BseNI ACTGG 3 cut(s) 153, 606, 936
BseXI GCAGC 3 cut(s) 654, 1105, 1152
BseYI CCCAGC 1 cut(s) 724
Bsh1236I CGCG 3 cut(s) 509, 564, 1087
Bsh1285I CGRYCG 2 cut(s) 275, 525
BshFI GGCC 3 cut(s) 32, 500, 802
BshVI ATCGAT 1 cut(s) 824
BsiEI CGRYCG 2 cut(s) 275, 525
BsiHKCI CYCGRG 1 cut(s) 636
BsiSI CCGG 6 cut(s) 9, 622, 637, 795, 832, 1309
BslFI GGGAC 3 cut(s) 358, 950, 1075
BslI CCNNNNNNNGG 2 cut(s) 643, 798
BsmAI GTCTC 3 cut(s) 348, 354, 793
BsmBI CGTCTC 1 cut(s) 348
BsmFI GGGAC 3 cut(s) 358, 950, 1075
BsnI GGCC 3 cut(s) 32, 500, 802
BsoBI CYCGRG 1 cut(s) 636
Bsp119I TTCGAA 1 cut(s) 615
Bsp1286I GDGCHC 1 cut(s) 543
Bsp143I GATC 2 cut(s) 595, 1179
Bsp19I CCATGG 2 cut(s) 549, 959
BspANI GGCC 3 cut(s) 32, 500, 802
BspCNI CTCAG 3 cut(s) 760, 891, 934
BspDI ATCGAT 1 cut(s) 824
BspFNI CGCG 3 cut(s) 509, 564, 1087
BspLI GGNNCC 1 cut(s) 374
BspMI ACCTGC 1 cut(s) 566
BspT104I TTCGAA 1 cut(s) 615
BsrBI CCGCTC 1 cut(s) 1276
BsrDI GCAATG 3 cut(s) 58, 740, 1053
BsrFI RCCGGY 1 cut(s) 8
BsrI ACTGG 3 cut(s) 153, 606, 936
BssAI RCCGGY 1 cut(s) 8
BssECI CCNNGG 5 cut(s) 502, 549, 636, 867, 959
BssMI GATC 2 cut(s) 595, 1179
BssT1I CCWWGG 2 cut(s) 549, 959
Bst2UI CCWGG 2 cut(s) 497, 868
Bst6I CTCTTC 2 cut(s) 170, 1113
BstBI TTCGAA 1 cut(s) 615
BstC8I GCNNGC 4 cut(s) 10, 511, 1193, 1274
BstDEI CTNAG 4 cut(s) 768, 878, 921, 1319
BstDSI CCRYGG 3 cut(s) 502, 549, 959
BstF5I GGATG 4 cut(s) 803, 994, 1284, 1291
BstFNI CGCG 3 cut(s) 509, 564, 1087
BstH2I RGCGCY 1 cut(s) 535
BstHHI GCGC 4 cut(s) 509, 534, 566, 1087
BstKTI GATC 2 cut(s) 598, 1182
BstMAI GTCTC 3 cut(s) 348, 354, 793
BstMBI GATC 2 cut(s) 595, 1179
BstMCI CGRYCG 2 cut(s) 275, 525
BstMWI GCNNNNNNNGC 9 cut(s) 21, 344, 506, 538, 572, 841, 1028, 1093, 1314
BstNI CCWGG 2 cut(s) 497, 868
BstNSI RCATGY 1 cut(s) 391
BstSCI CCNGG 6 cut(s) 495, 635, 636, 794, 866, 1308
BstUI CGCG 3 cut(s) 509, 564, 1087
BstV1I GCAGC 3 cut(s) 654, 1105, 1152
BstXI CCANNNNNNTGG 1 cut(s) 148
Bsu15I ATCGAT 1 cut(s) 824
BsuI GTATCC 1 cut(s) 547
BsuRI GGCC 3 cut(s) 32, 500, 802
BsuTUI ATCGAT 1 cut(s) 824
BtgI CCRYGG 3 cut(s) 502, 549, 959
BtrI CACGTC 1 cut(s) 144
BtsCI GGATG 4 cut(s) 803, 994, 1284, 1291
BtsI GCAGTG 2 cut(s) 762, 1115
BtsIMutI CAGTG 4 cut(s) 146, 762, 886, 1115
BveI ACCTGC 1 cut(s) 566
Cac8I GCNNGC 4 cut(s) 10, 511, 1193, 1274
CfoI GCGC 4 cut(s) 509, 534, 566, 1087
Cfr10I RCCGGY 1 cut(s) 8
Cfr13I GGNCC 3 cut(s) 372, 499, 884
Cfr9I CCCGGG 1 cut(s) 636
ClaI ATCGAT 1 cut(s) 824
Csp6I GTAC 2 cut(s) 27, 925
CviAII CATG 5 cut(s) 388, 550, 811, 960, 1297
CviQI GTAC 2 cut(s) 27, 925
DdeI CTNAG 4 cut(s) 768, 878, 921, 1319
DpnI GATC 2 cut(s) 597, 1181
DpnII GATC 2 cut(s) 595, 1179
EaeI YGGCCR 2 cut(s) 30, 800
Eam1104I CTCTTC 2 cut(s) 170, 1113
EarI CTCTTC 2 cut(s) 170, 1113
EciI GGCGGA 5 cut(s) 30, 217, 325, 781, 1010
Eco130I CCWWGG 2 cut(s) 549, 959
Eco24I GRGCYC 1 cut(s) 543
Eco47I GGWCC 2 cut(s) 372, 884
Eco88I CYCGRG 1 cut(s) 636
EcoRI GAATTC 2 cut(s) 617, 910
EcoRII CCWGG 2 cut(s) 495, 866
EcoT14I CCWWGG 2 cut(s) 549, 959
EcoT38I GRGCYC 1 cut(s) 543
ErhI CCWWGG 2 cut(s) 549, 959
Esp3I CGTCTC 1 cut(s) 348
FaeI CATG 5 cut(s) 391, 553, 814, 963, 1300
FaqI GGGAC 3 cut(s) 358, 950, 1075
FatI CATG 5 cut(s) 387, 549, 810, 959, 1296
FbaI TGATCA 1 cut(s) 1179
FokI GGATG 4 cut(s) 790, 1001, 1291, 1298
FriOI GRGCYC 1 cut(s) 543
FspBI CTAG 1 cut(s) 1104
GlaI GCGC 4 cut(s) 508, 533, 565, 1086
GsaI CCCAGC 1 cut(s) 728
GsuI CTGGAG 1 cut(s) 427
HaeII RGCGCY 1 cut(s) 535
HaeIII GGCC 3 cut(s) 32, 500, 802
HapII CCGG 6 cut(s) 9, 622, 637, 795, 832, 1309
HhaI GCGC 4 cut(s) 509, 534, 566, 1087
Hin1II CATG 5 cut(s) 391, 553, 814, 963, 1300
Hin6I GCGC 4 cut(s) 507, 532, 564, 1085
HinP1I GCGC 4 cut(s) 507, 532, 564, 1085
HindIII AAGCTT 1 cut(s) 842
HinfI GANTC 7 cut(s) 18, 212, 447, 764, 821, 863, 938
HpaII CCGG 6 cut(s) 9, 622, 637, 795, 832, 1309
HphI GGTGA 3 cut(s) 273, 601, 728
Hpy166II GTNNAC 3 cut(s) 246, 592, 1261
Hpy188I TCNGA 8 cut(s) 394, 527, 628, 769, 988, 1179, 1204, 1322
Hpy188III TCNNGA 3 cut(s) 444, 699, 782
Hpy8I GTNNAC 3 cut(s) 246, 592, 1261
HpyAV CCTTC 7 cut(s) 169, 197, 412, 462, 522, 1012, 1108
HpyCH4IV ACGT 1 cut(s) 143
HpyCH4V TGCA 3 cut(s) 1036, 1168, 1300
HpyF10VI GCNNNNNNNGC 9 cut(s) 21, 344, 506, 538, 572, 841, 1028, 1093, 1314
HpyF3I CTNAG 4 cut(s) 768, 878, 921, 1319
HpySE526I ACGT 1 cut(s) 143
Hsp92II CATG 5 cut(s) 391, 553, 814, 963, 1300
HspAI GCGC 4 cut(s) 507, 532, 564, 1085
KroI GCCGGC 1 cut(s) 8
KroNI GCCGGC 1 cut(s) 10
Ksp22I TGATCA 1 cut(s) 1179
Kzo9I GATC 2 cut(s) 595, 1179
LmnI GCTCC 1 cut(s) 1234
Lsp1109I GCAGC 3 cut(s) 654, 1105, 1152
MaeI CTAG 1 cut(s) 1104
MaeII ACGT 1 cut(s) 143
MaeIII GTNAC 3 cut(s) 130, 144, 1150
MalI GATC 2 cut(s) 597, 1181
MbiI CCGCTC 1 cut(s) 1276
MboI GATC 2 cut(s) 595, 1179
MboII GAAGA 3 cut(s) 157, 373, 1130
MhlI GDGCHC 1 cut(s) 543
MlsI TGGCCA 1 cut(s) 802
MluCI AATT 7 cut(s) 466, 486, 617, 673, 719, 872, 910
MluNI TGGCCA 1 cut(s) 802
MlyI GAGTC 3 cut(s) 27, 872, 932
MmeI TCCRAC 2 cut(s) 505, 966
Mox20I TGGCCA 1 cut(s) 802
MroNI GCCGGC 1 cut(s) 8
MroXI GAANNNNTTC 2 cut(s) 451, 611
MscI TGGCCA 1 cut(s) 802
MseI TTAA 2 cut(s) 1026, 1223
Msp20I TGGCCA 1 cut(s) 802
MspA1I CMGCKG 1 cut(s) 369
MspI CCGG 6 cut(s) 9, 622, 637, 795, 832, 1309
MspR9I CCNGG 6 cut(s) 497, 637, 638, 796, 868, 1310
MvaI CCWGG 2 cut(s) 497, 868
MvnI CGCG 3 cut(s) 509, 564, 1087
MwoI GCNNNNNNNGC 9 cut(s) 21, 344, 506, 538, 572, 841, 1028, 1093, 1314
NaeI GCCGGC 1 cut(s) 10
NciI CCSGG 4 cut(s) 637, 638, 796, 1310
NcoI CCATGG 2 cut(s) 549, 959
NdeII GATC 2 cut(s) 595, 1179
NgoMIV GCCGGC 1 cut(s) 8
NlaIII CATG 5 cut(s) 391, 553, 814, 963, 1300
NlaIV GGNNCC 1 cut(s) 374
NmuCI GTSAC 1 cut(s) 144
NspI RCATGY 1 cut(s) 391
NspV TTCGAA 1 cut(s) 615
PcsI WCGNNNNNNNCGW 2 cut(s) 126, 1269
PdiI GCCGGC 1 cut(s) 10
PdmI GAANNNNTTC 2 cut(s) 451, 611
PfeI GAWTC 4 cut(s) 212, 447, 764, 821
PleI GAGTC 3 cut(s) 26, 871, 932
PpsI GAGTC 3 cut(s) 26, 871, 932
Psp6I CCWGG 2 cut(s) 495, 866
PspFI CCCAGC 1 cut(s) 724
PspGI CCWGG 2 cut(s) 495, 866
PspN4I GGNNCC 1 cut(s) 374
PspPI GGNCC 3 cut(s) 372, 499, 884
RsaI GTAC 2 cut(s) 28, 926
RsaNI GTAC 2 cut(s) 27, 925
SaqAI TTAA 2 cut(s) 1026, 1223
Sau3AI GATC 2 cut(s) 595, 1179
Sau96I GGNCC 3 cut(s) 372, 499, 884
SchI GAGTC 3 cut(s) 27, 872, 932
ScrFI CCNGG 6 cut(s) 497, 637, 638, 796, 868, 1310
SduI GDGCHC 1 cut(s) 543
SfuI TTCGAA 1 cut(s) 615
SinI GGWCC 2 cut(s) 372, 884
SmaI CCCGGG 1 cut(s) 638
SmlI CTYRAG 1 cut(s) 1303
SmoI CTYRAG 1 cut(s) 1303
Sse9I AATT 7 cut(s) 466, 486, 617, 673, 719, 872, 910
SspMI CTAG 1 cut(s) 1104
StyD4I CCNGG 6 cut(s) 495, 635, 636, 794, 866, 1308
StyI CCWWGG 2 cut(s) 549, 959
TaiI ACGT 1 cut(s) 146
TaqI TCGA 4 cut(s) 615, 698, 783, 824
TasI AATT 7 cut(s) 466, 486, 617, 673, 719, 872, 910
TatI WGTACW 1 cut(s) 924
TfiI GAWTC 4 cut(s) 212, 447, 764, 821
Tru1I TTAA 2 cut(s) 1026, 1223
Tru9I TTAA 2 cut(s) 1026, 1223
TscAI CASTG 4 cut(s) 153, 762, 886, 1115
TseFI GTSAC 1 cut(s) 144
TseI GCWGC 4 cut(s) 5, 667, 1093, 1165
Tsp45I GTSAC 1 cut(s) 144
TspDTI ATGAA 1 cut(s) 827
TspGWI ACGGA 3 cut(s) 366, 372, 397
TspMI CCCGGG 1 cut(s) 636
TspRI CASTG 4 cut(s) 153, 762, 886, 1115
VpaK11BI GGWCC 2 cut(s) 372, 884
XapI RAATTY 2 cut(s) 617, 910
XceI RCATGY 1 cut(s) 391
XmaI CCCGGG 1 cut(s) 636
XmnI GAANNNNTTC 2 cut(s) 451, 611
XspI CTAG 1 cut(s) 1104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.