Rh2AG024400

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0009592
Physical Location & Seq
Reverse (-)
1714212 .. 1715156
945 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG024400.1

Sequence Viewer

Length: 945 bp
ATGTCCGAACACTCGTTCTCTATCCTTCTCTCCATCCTCTCCCCGTTTCTGACCTCCGCAATCCCTTCGATTCCGCCCAACTACGCCCTCGCCGCCGCGATTTACCGCCTCGCCCACGGCGCCACCTACAAGGCGGTCGGCCGGCGCTTCGGGATGGACTCGGCGGAGGCCTGCCGGGCTTTCTACGCCGTCTGCAAGTCGGTCAATGATAAATTAGGGAGCTTGTTTGAGCTCCGGTCCGATATGGCTCGGATTGTTGGGGGTTTCGGGTGGATTTCGCTGCCGAATTGCTGTGGGGTGTTGGGGTTTGGGAGATTTGGGGTTAGTGGTGAAATGCTGGGGCCAAATGGGTCGCTGTTGGTTCAGGCATTGGTGGATTCTGAAGGGAGGTTTCTGGATGTTTCGGCTGGCTGGCCGAGCACAGTGAAACCGGAAGCGATTTTCCGGCAGAGTAAGCTGTATTTGGGTGTGGAGGAGTCCGGGGAGTTGTTGAATGGTCCTGTTTATGAGCTCAGTGATGGCAATGCGCTTCCTCAGTACATGCTGGGGGACTCTTGCTTTCCTCTGCTGCCTTGGCTTTTGACTCCGTATGTGAGATCAAATGAGGAAGAGGAGAGTTTCGGTACATTGGAGAAGGCGTTTAACTCAGCGCATGGTCGCGCAATGGGGCTGGTTGGGACGGCGTTTGCGAGGGTGAGGGCTCGGTGGCAGCTGCTGAGTAGGGAGTGGAAGGAAGAGTGTGTGGAGTTTTTGCCGTTTGTGGTGGTTACTGGCTGTCTGCTGAATAATTTTCTGATGAAGTGTAGTGAGCCGCTGCCGGATGACAATGTGGGGTGCTTGAGGGAGGAGGAGCTTCAGGTGTTCGAAGGACAGGTGGATGAGAGTGGCGAGAGGATTAGGGATGTGCTTGCCACTTACTTGAGCCGGGTAAGCCTAAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

314

Amino Acids

34.56

Weight (kDa)

5.13

Isoelectric Point (pI)

49.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 118 - 263 1.6e-15 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 119
AccII CGCG 2 cut(s) 98, 660
AciI CCGC 8 cut(s) 57, 74, 93, 96, 106, 134, 164, 812
AcoI YGGCCR 2 cut(s) 139, 413
AcuI CTGAAG 2 cut(s) 402, 839
AcyI GRCGYC 1 cut(s) 120
AfaI GTAC 2 cut(s) 539, 625
AgsI TTSAA 1 cut(s) 493
AluBI AGCT 6 cut(s) 222, 232, 457, 511, 712, 853
AluI AGCT 6 cut(s) 222, 232, 457, 511, 712, 853
Alw21I GWGCWC 3 cut(s) 234, 422, 513
AlwNI CAGNNNCTG 1 cut(s) 715
AoxI GGCC 4 cut(s) 139, 168, 341, 413
ApeKI GCWGC 5 cut(s) 280, 568, 709, 712, 814
AspLEI GCGC 5 cut(s) 122, 147, 529, 652, 662
AspS9I GGNCC 3 cut(s) 237, 341, 497
AsuC2I CCSGG 3 cut(s) 176, 481, 926
AsuHPI GGTGA 2 cut(s) 341, 706
AsuII TTCGAA 1 cut(s) 864
AvaII GGWCC 2 cut(s) 237, 497
BanI GGYRCC 1 cut(s) 119
BanII GRGCYC 3 cut(s) 234, 513, 703
Bbv12I GWGCWC 3 cut(s) 234, 422, 513
BbvI GCAGC 5 cut(s) 267, 555, 699, 721, 801
BccI CCATC 3 cut(s) 41, 148, 512
BceAI ACGGC 4 cut(s) 133, 173, 696, 739
BcgI CGANNNNNNTGC 2 cut(s) 48, 82
BcnI CCSGG 3 cut(s) 176, 481, 926
BfoI RGCGCY 2 cut(s) 123, 148
BglI GCCNNNNNGGC 1 cut(s) 176
BisI GCNGC 8 cut(s) 93, 96, 281, 569, 710, 713, 812, 815
BlsI GCNGC 8 cut(s) 94, 97, 282, 570, 711, 714, 813, 816
Bme1390I CCNGG 3 cut(s) 176, 481, 926
Bme18I GGWCC 2 cut(s) 237, 497
BmgT120I GGNCC 3 cut(s) 237, 341, 497
BmiI GGNNCC 2 cut(s) 121, 342
BmrFI CCNGG 3 cut(s) 176, 481, 926
Bpu14I TTCGAA 1 cut(s) 864
BpuEI CTTGAG 2 cut(s) 859, 940
BpuMI CCSGG 3 cut(s) 176, 481, 926
BsaHI GRCGYC 1 cut(s) 120
BsaJI CCNNGG 3 cut(s) 115, 480, 572
BsaWI WCCGGW 2 cut(s) 234, 430
BsaXI ACNNNNNCTCC 2 cut(s) 623, 653
Bse118I RCCGGY 1 cut(s) 141
Bse1I ACTGG 1 cut(s) 775
Bse3DI GCAATG 2 cut(s) 529, 669
BseDI CCNNGG 3 cut(s) 115, 480, 572
BseGI GGATG 6 cut(s) 33, 159, 403, 826, 883, 907
BseMI GCAATG 2 cut(s) 529, 669
BseMII CTCAG 4 cut(s) 526, 548, 660, 707
BseNI ACTGG 1 cut(s) 775
BseRI GAGGAG 4 cut(s) 488, 626, 860, 863
BseX3I CGGCCG 1 cut(s) 139
BseXI GCAGC 5 cut(s) 267, 555, 699, 721, 801
BseYI CCCAGC 2 cut(s) 337, 544
Bsh1236I CGCG 2 cut(s) 98, 660
Bsh1285I CGRYCG 2 cut(s) 138, 142
BshFI GGCC 4 cut(s) 141, 170, 343, 415
BshNI GGYRCC 1 cut(s) 119
BsiEI CGRYCG 2 cut(s) 138, 142
BsiHKAI GWGCWC 3 cut(s) 234, 422, 513
BsiSI CCGG 8 cut(s) 142, 175, 235, 431, 445, 480, 818, 925
BslFI GGGAC 2 cut(s) 563, 691
BsmFI GGGAC 2 cut(s) 563, 691
BsnI GGCC 4 cut(s) 141, 170, 343, 415
Bsp119I TTCGAA 1 cut(s) 864
Bsp1286I GDGCHC 4 cut(s) 234, 422, 513, 703
Bsp143I GATC 1 cut(s) 596
BspACI CCGC 8 cut(s) 57, 74, 93, 96, 106, 134, 164, 812
BspANI GGCC 4 cut(s) 141, 170, 343, 415
BspCNI CTCAG 4 cut(s) 525, 547, 659, 708
BspFNI CGCG 2 cut(s) 98, 660
BspLI GGNNCC 2 cut(s) 121, 342
BspT104I TTCGAA 1 cut(s) 864
BspT107I GGYRCC 1 cut(s) 119
BsrDI GCAATG 2 cut(s) 529, 669
BsrFI RCCGGY 1 cut(s) 141
BsrI ACTGG 1 cut(s) 775
BssAI RCCGGY 1 cut(s) 141
BssECI CCNNGG 3 cut(s) 115, 480, 572
BssMI GATC 1 cut(s) 596
BssNI GRCGYC 1 cut(s) 120
BssT1I CCWWGG 1 cut(s) 572
Bst4CI ACNGT 1 cut(s) 424
Bst6I CTCTTC 2 cut(s) 603, 729
BstACI GRCGYC 1 cut(s) 120
BstBI TTCGAA 1 cut(s) 864
BstC8I GCNNGC 5 cut(s) 143, 172, 409, 413, 909
BstDEI CTNAG 5 cut(s) 512, 534, 646, 716, 935
BstDSI CCRYGG 1 cut(s) 115
BstF5I GGATG 6 cut(s) 33, 159, 403, 826, 883, 907
BstFNI CGCG 2 cut(s) 98, 660
BstH2I RGCGCY 2 cut(s) 123, 148
BstHHI GCGC 5 cut(s) 122, 147, 529, 652, 662
BstKTI GATC 1 cut(s) 599
BstMBI GATC 1 cut(s) 596
BstMCI CGRYCG 2 cut(s) 138, 142
BstMWI GCNNNNNNNGC 8 cut(s) 92, 119, 176, 185, 417, 454, 574, 930
BstNSI RCATGY 1 cut(s) 544
BstSCI CCNGG 3 cut(s) 174, 479, 924
BstUI CGCG 2 cut(s) 98, 660
BstV1I GCAGC 5 cut(s) 267, 555, 699, 721, 801
BstZI CGGCCG 1 cut(s) 139
BsuRI GGCC 4 cut(s) 141, 170, 343, 415
BtgI CCRYGG 1 cut(s) 115
BtsCI GGATG 6 cut(s) 33, 159, 403, 826, 883, 907
BtsIMutI CAGTG 2 cut(s) 429, 520
Cac8I GCNNGC 5 cut(s) 143, 172, 409, 413, 909
CaiI CAGNNNCTG 1 cut(s) 715
CfoI GCGC 5 cut(s) 122, 147, 529, 652, 662
Cfr10I RCCGGY 1 cut(s) 141
Cfr13I GGNCC 3 cut(s) 237, 341, 497
CpoI CGGWCCG 1 cut(s) 237
Csp6I GTAC 2 cut(s) 538, 624
CspI CGGWCCG 1 cut(s) 237
CviAII CATG 2 cut(s) 541, 653
CviQI GTAC 2 cut(s) 538, 624
DdeI CTNAG 5 cut(s) 512, 534, 646, 716, 935
DinI GGCGCC 1 cut(s) 121
DpnI GATC 1 cut(s) 598
DpnII GATC 1 cut(s) 596
EaeI YGGCCR 2 cut(s) 139, 413
EagI CGGCCG 1 cut(s) 139
Eam1104I CTCTTC 2 cut(s) 603, 729
EarI CTCTTC 2 cut(s) 603, 729
EciI GGCGGA 2 cut(s) 63, 179
Ecl136II GAGCTC 2 cut(s) 232, 511
EclXI CGGCCG 1 cut(s) 139
Eco130I CCWWGG 1 cut(s) 572
Eco147I AGGCCT 1 cut(s) 170
Eco24I GRGCYC 3 cut(s) 234, 513, 703
Eco47I GGWCC 2 cut(s) 237, 497
Eco52I CGGCCG 1 cut(s) 139
Eco53kI GAGCTC 2 cut(s) 232, 511
Eco57I CTGAAG 2 cut(s) 402, 839
EcoICRI GAGCTC 2 cut(s) 232, 511
EcoT14I CCWWGG 1 cut(s) 572
EcoT38I GRGCYC 3 cut(s) 234, 513, 703
EgeI GGCGCC 1 cut(s) 121
EheI GGCGCC 1 cut(s) 121
ErhI CCWWGG 1 cut(s) 572
FaeI CATG 2 cut(s) 544, 656
FaiI YATR 5 cut(s) 245, 507, 542, 591, 654
FaqI GGGAC 2 cut(s) 563, 691
FatI CATG 2 cut(s) 540, 652
Fnu4HI GCNGC 8 cut(s) 93, 96, 281, 569, 710, 713, 812, 815
FokI GGATG 6 cut(s) 20, 166, 410, 833, 890, 914
FriOI GRGCYC 3 cut(s) 234, 513, 703
Fsp4HI GCNGC 8 cut(s) 93, 96, 281, 569, 710, 713, 812, 815
GlaI GCGC 5 cut(s) 121, 146, 528, 651, 661
GluI GCNGC 8 cut(s) 93, 96, 281, 569, 710, 713, 812, 815
GsaI CCCAGC 2 cut(s) 341, 548
HaeII RGCGCY 2 cut(s) 123, 148
HaeIII GGCC 4 cut(s) 141, 170, 343, 415
HapII CCGG 8 cut(s) 142, 175, 235, 431, 445, 480, 818, 925
HhaI GCGC 5 cut(s) 122, 147, 529, 652, 662
Hin1I GRCGYC 1 cut(s) 120
Hin1II CATG 2 cut(s) 544, 656
Hin6I GCGC 5 cut(s) 120, 145, 527, 650, 660
HinP1I GCGC 5 cut(s) 120, 145, 527, 650, 660
HinfI GANTC 6 cut(s) 70, 158, 377, 476, 551, 583
HpaII CCGG 8 cut(s) 142, 175, 235, 431, 445, 480, 818, 925
HphI GGTGA 2 cut(s) 341, 706
Hpy188I TCNGA 6 cut(s) 7, 51, 241, 252, 382, 795
Hpy188III TCNNGA 2 cut(s) 151, 395
HpyAV CCTTC 6 cut(s) 35, 75, 377, 628, 724, 860
HpyCH4III ACNGT 1 cut(s) 424
HpyCH4V TGCA 1 cut(s) 195
HpyF10VI GCNNNNNNNGC 8 cut(s) 92, 119, 176, 185, 417, 454, 574, 930
HpyF3I CTNAG 5 cut(s) 512, 534, 646, 716, 935
Hsp92I GRCGYC 1 cut(s) 120
Hsp92II CATG 2 cut(s) 544, 656
HspAI GCGC 5 cut(s) 120, 145, 527, 650, 660
KasI GGCGCC 1 cut(s) 119
KroI GCCGGC 1 cut(s) 141
KroNI GCCGGC 1 cut(s) 143
Kzo9I GATC 1 cut(s) 596
LmnI GCTCC 3 cut(s) 219, 237, 850
Lsp1109I GCAGC 5 cut(s) 267, 555, 699, 721, 801
MaeIII GTNAC 1 cut(s) 766
MalI GATC 1 cut(s) 598
MboI GATC 1 cut(s) 596
MboII GAAGA 2 cut(s) 620, 746
MhlI GDGCHC 4 cut(s) 234, 422, 513, 703
MluCI AATT 3 cut(s) 212, 286, 787
Mly113I GGCGCC 1 cut(s) 120
MlyI GAGTC 4 cut(s) 152, 485, 545, 577
MroNI GCCGGC 1 cut(s) 141
MseI TTAA 1 cut(s) 642
MspA1I CMGCKG 2 cut(s) 712, 814
MspI CCGG 8 cut(s) 142, 175, 235, 431, 445, 480, 818, 925
MspR9I CCNGG 3 cut(s) 176, 481, 926
MvnI CGCG 2 cut(s) 98, 660
MwoI GCNNNNNNNGC 8 cut(s) 92, 119, 176, 185, 417, 454, 574, 930
NaeI GCCGGC 1 cut(s) 143
NarI GGCGCC 1 cut(s) 120
NciI CCSGG 3 cut(s) 176, 481, 926
NdeII GATC 1 cut(s) 596
NgoMIV GCCGGC 1 cut(s) 141
NlaIII CATG 2 cut(s) 544, 656
NlaIV GGNNCC 2 cut(s) 121, 342
NmeAIII GCCGAG 2 cut(s) 140, 441
NspI RCATGY 1 cut(s) 544
NspV TTCGAA 1 cut(s) 864
PceI AGGCCT 1 cut(s) 170
PcsI WCGNNNNNNNCGW 1 cut(s) 686
PdiI GCCGGC 1 cut(s) 143
PfeI GAWTC 2 cut(s) 70, 377
PkrI GCNGC 8 cut(s) 94, 97, 282, 570, 711, 714, 813, 816
PleI GAGTC 4 cut(s) 152, 484, 545, 577
PluTI GGCGCC 1 cut(s) 123
PpsI GAGTC 4 cut(s) 152, 484, 545, 577
Psp124BI GAGCTC 2 cut(s) 234, 513
PspFI CCCAGC 2 cut(s) 337, 544
PspN4I GGNNCC 2 cut(s) 121, 342
PspPI GGNCC 3 cut(s) 237, 341, 497
PstNI CAGNNNCTG 1 cut(s) 715
PvuII CAGCTG 1 cut(s) 712
RsaI GTAC 2 cut(s) 539, 625
RsaNI GTAC 2 cut(s) 538, 624
Rsr2I CGGWCCG 1 cut(s) 237
RsrII CGGWCCG 1 cut(s) 237
SacI GAGCTC 2 cut(s) 234, 513
SaqAI TTAA 1 cut(s) 642
SatI GCNGC 8 cut(s) 93, 96, 281, 569, 710, 713, 812, 815
Sau3AI GATC 1 cut(s) 596
Sau96I GGNCC 3 cut(s) 237, 341, 497
SchI GAGTC 4 cut(s) 152, 485, 545, 577
ScrFI CCNGG 3 cut(s) 176, 481, 926
SduI GDGCHC 4 cut(s) 234, 422, 513, 703
SfoI GGCGCC 1 cut(s) 121
SfuI TTCGAA 1 cut(s) 864
SinI GGWCC 2 cut(s) 237, 497
SmlI CTYRAG 2 cut(s) 838, 919
SmoI CTYRAG 2 cut(s) 838, 919
Sse9I AATT 3 cut(s) 212, 286, 787
SseBI AGGCCT 1 cut(s) 170
SsiI CCGC 8 cut(s) 57, 74, 93, 96, 106, 134, 164, 812
SspDI GGCGCC 1 cut(s) 119
SstI GAGCTC 2 cut(s) 234, 513
StuI AGGCCT 1 cut(s) 170
StyD4I CCNGG 3 cut(s) 174, 479, 924
StyI CCWWGG 1 cut(s) 572
TaaI ACNGT 1 cut(s) 424
TaqI TCGA 2 cut(s) 68, 864
TaqII GACCGA 1 cut(s) 190
TasI AATT 3 cut(s) 212, 286, 787
TatI WGTACW 1 cut(s) 537
TauI GCSGC 3 cut(s) 95, 98, 814
TfiI GAWTC 2 cut(s) 70, 377
Tru1I TTAA 1 cut(s) 642
Tru9I TTAA 1 cut(s) 642
TscAI CASTG 2 cut(s) 429, 520
TseI GCWGC 5 cut(s) 280, 568, 709, 712, 814
TspDTI ATGAA 1 cut(s) 812
TspGWI ACGGA 1 cut(s) 576
TspRI CASTG 2 cut(s) 429, 520
VpaK11BI GGWCC 2 cut(s) 237, 497
XceI RCATGY 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.