Rh2BG024900

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0009592
Physical Location & Seq
Reverse (-)
1666832 .. 1668725
1894 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG024900.1

Sequence Viewer

Length: 1308 bp
ATGGCCGCCGTCAGCGGCGGCGGAGAAGCCAAGAACACCGCCAAACGCGGCAAGCAGAAGAACAAGAACCACCACACTCCGCCGCTCGATCAACGAAACCTCCTCAGCCTCGTCACCTCCGCCACCTCACTCGCCCACTCCTTCCTCTCCCAAAACGACCTCCTCCTCCTCCCCTCCCAAACCCTAACCCTAGAAACCCTCCTCTCCTCCGCCTCCTCCGCCCTCTCCCCCCTCCTCCTCCTCTCATCTCCGCCGCCGCCGCAACCACCTCCGCCTCCTCCTCCTCCTCAAGAATGCTGGTTCCGCCGCTTCCTCTCCTCCTCCGCCGGCGACTCGCCGTGGCGCCACGCCTTCCGCATGTCCGAACACTCGTTCTCTATCCTTCTCTCCATCCTCTCCCCATTTCTGACCTCCGCAATCCCTTCGATTCCGCCCAACTACGCCCTCGCCGCCGCGATCTACCGCCTCGCCCACGGCGCCACCTACAAGGCCGCCGGCCGCCGCTTCGGGATGGACTCCGCCGAGGCCTGCCGGGCTTTCTACGCCGTCTGCAAGTCGGTCAATGATAAATTAGGGAGCTTGTTTGAGCTCCGGTCCGATATGGCTGGAATTGCTGGGGGTTTCCGGTGGATTTCGCTGCCGAATTGCTGTGGGGTGTTAGGGTTTGGGAGATTTGGGGTTAGTGGTGAAATGCTGGGGCCGAATGGGTCGCTGTTGGTTCAGGCATTGGTGGATTCCGAAGGGAGGTTTCTGGATGTTTCGGCTGGCTGGCCGAGCACAGTGAAACCGGAAGCGATTTTCCGGCAGAGTAAGCTGTATTTGGGTGTGGAGGAGTCCAGGGAGTTGTTGAATGGTCCTGTTCATGAGCTCAGTGATGGCAATGCGCTTCCTCAGTACATATTGGGGGAGTCTTGCTTTCCTCTGTTGCCTTGGCTTTTGACTCCGTATGTGAGATCAAATGAGGAAGGGGAGAGTTTCGGTACATTGGAGAAGGCGTTTAACTCGGCGCATGGTCGCGCAATGGGGCTGGTTGGGACGGCGTTTGGGAGGGTGAGGGCTCGGTGGCAGCTGCTGAGTAGGGAGTGGAAGGAAGAGTGTGTGGAGTTTTTGCCGTTTGTGGTGGTGACTGGCTGTCTGCTGAATAATTTTCTGATGAAGTGTAGTGAGCCGCTGCCGGATGACAATGTGGGGTGCTTGAGGGAGGAGGAGGAGCTTCTTCAGGTGTTCGAAGGACAGGTGGATGAGAGTGGCGAGAGGATTAGGGATGTGCTTGCCACGCACTTGAGCCGGGTAAGCCTAAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

435

Amino Acids

47.48

Weight (kDa)

6.53

Isoelectric Point (pI)

58.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 237 - 382 6.1e-15 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 342, 476
AccBSI CCGCTC 1 cut(s) 85
AccII CGCG 3 cut(s) 48, 455, 1017
AcoI YGGCCR 3 cut(s) 3, 496, 770
AcuI CTGAAG 1 cut(s) 1202
AcyI GRCGYC 2 cut(s) 343, 477
AfaI GTAC 2 cut(s) 896, 982
AfiI CCNNNNNNNGG 1 cut(s) 744
AgsI TTSAA 1 cut(s) 850
AhdI GACNNNNNGTC 1 cut(s) 1131
AjnI CCWGG 1 cut(s) 836
AluBI AGCT 6 cut(s) 579, 589, 814, 868, 1069, 1213
AluI AGCT 6 cut(s) 579, 589, 814, 868, 1069, 1213
Alw21I GWGCWC 3 cut(s) 591, 779, 870
AlwNI CAGNNNCTG 1 cut(s) 1072
AoxI GGCC 6 cut(s) 3, 489, 496, 525, 698, 770
ApeKI GCWGC 4 cut(s) 637, 1066, 1069, 1171
AspLEI GCGC 5 cut(s) 345, 479, 886, 1009, 1019
AspS9I GGNCC 3 cut(s) 594, 698, 854
AsuC2I CCSGG 2 cut(s) 533, 1289
AsuHPI GGTGA 4 cut(s) 106, 698, 1063, 1135
AsuII TTCGAA 1 cut(s) 1227
AvaII GGWCC 2 cut(s) 594, 854
BanI GGYRCC 2 cut(s) 342, 476
BanII GRGCYC 3 cut(s) 591, 870, 1060
Bbv12I GWGCWC 3 cut(s) 591, 779, 870
BbvCI CCTCAGC 1 cut(s) 104
BbvI GCAGC 4 cut(s) 624, 1056, 1078, 1158
BccI CCATC 3 cut(s) 398, 505, 869
BceAI ACGGC 5 cut(s) 322, 490, 530, 1053, 1096
BcgI CGANNNNNNTGC 2 cut(s) 405, 439
BciT130I CCWGG 1 cut(s) 838
BcnI CCSGG 2 cut(s) 533, 1289
BfaI CTAG 1 cut(s) 191
BfoI RGCGCY 2 cut(s) 346, 480
BglI GCCNNNNNGGC 1 cut(s) 533
Bme1390I CCNGG 3 cut(s) 533, 838, 1289
Bme18I GGWCC 2 cut(s) 594, 854
BmeRI GACNNNNNGTC 1 cut(s) 1131
BmgT120I GGNCC 3 cut(s) 594, 698, 854
BmiI GGNNCC 4 cut(s) 302, 344, 478, 699
BmrFI CCNGG 3 cut(s) 533, 838, 1289
Bpu10I CCTNAGC 1 cut(s) 104
Bpu14I TTCGAA 1 cut(s) 1227
BpuEI CTTGAG 3 cut(s) 273, 1216, 1303
BpuMI CCSGG 2 cut(s) 533, 1289
BsaHI GRCGYC 2 cut(s) 343, 477
BsaJI CCNNGG 5 cut(s) 338, 472, 522, 837, 929
BsaWI WCCGGW 3 cut(s) 591, 624, 787
BsaXI ACNNNNNCTCC 4 cut(s) 84, 114, 980, 1010
Bsc4I CCNNNNNNNGG 1 cut(s) 744
Bse118I RCCGGY 2 cut(s) 326, 494
Bse1I ACTGG 1 cut(s) 1132
Bse3DI GCAATG 2 cut(s) 886, 1026
BseBI CCWGG 1 cut(s) 838
BseDI CCNNGG 5 cut(s) 338, 472, 522, 837, 929
BseGI GGATG 6 cut(s) 390, 516, 760, 1183, 1246, 1270
BseLI CCNNNNNNNGG 1 cut(s) 744
BseMI GCAATG 2 cut(s) 886, 1026
BseMII CTCAG 4 cut(s) 118, 883, 905, 1064
BseNI ACTGG 1 cut(s) 1132
BseX3I CGGCCG 1 cut(s) 496
BseXI GCAGC 4 cut(s) 624, 1056, 1078, 1158
BseYI CCCAGC 2 cut(s) 614, 694
Bsh1236I CGCG 3 cut(s) 48, 455, 1017
Bsh1285I CGRYCG 1 cut(s) 499
BshFI GGCC 6 cut(s) 5, 491, 498, 527, 700, 772
BshNI GGYRCC 2 cut(s) 342, 476
BsiEI CGRYCG 1 cut(s) 499
BsiHKAI GWGCWC 3 cut(s) 591, 779, 870
BsiSI CCGG 9 cut(s) 327, 495, 532, 592, 625, 788, 802, 1175, 1288
BslFI GGGAC 1 cut(s) 1048
BslI CCNNNNNNNGG 1 cut(s) 744
BsmFI GGGAC 1 cut(s) 1048
BsmI GAATGC 1 cut(s) 299
BsnI GGCC 6 cut(s) 5, 491, 498, 527, 700, 772
Bsp119I TTCGAA 1 cut(s) 1227
Bsp1286I GDGCHC 4 cut(s) 591, 779, 870, 1060
Bsp143I GATC 3 cut(s) 88, 456, 953
BspANI GGCC 6 cut(s) 5, 491, 498, 527, 700, 772
BspCNI CTCAG 4 cut(s) 117, 882, 904, 1065
BspFNI CGCG 3 cut(s) 48, 455, 1017
BspHI TCATGA 1 cut(s) 862
BspLI GGNNCC 4 cut(s) 302, 344, 478, 699
BspT104I TTCGAA 1 cut(s) 1227
BspT107I GGYRCC 2 cut(s) 342, 476
BsrBI CCGCTC 1 cut(s) 85
BsrDI GCAATG 2 cut(s) 886, 1026
BsrFI RCCGGY 2 cut(s) 326, 494
BsrI ACTGG 1 cut(s) 1132
BssAI RCCGGY 2 cut(s) 326, 494
BssECI CCNNGG 5 cut(s) 338, 472, 522, 837, 929
BssMI GATC 3 cut(s) 88, 456, 953
BssNI GRCGYC 2 cut(s) 343, 477
BssT1I CCWWGG 1 cut(s) 929
Bst2UI CCWGG 1 cut(s) 838
Bst4CI ACNGT 1 cut(s) 781
Bst6I CTCTTC 1 cut(s) 1086
BstACI GRCGYC 2 cut(s) 343, 477
BstBI TTCGAA 1 cut(s) 1227
BstC8I GCNNGC 7 cut(s) 53, 328, 496, 529, 766, 770, 1272
BstDEI CTNAG 5 cut(s) 104, 869, 891, 1073, 1298
BstDSI CCRYGG 2 cut(s) 338, 472
BstF5I GGATG 6 cut(s) 390, 516, 760, 1183, 1246, 1270
BstFNI CGCG 3 cut(s) 48, 455, 1017
BstH2I RGCGCY 2 cut(s) 346, 480
BstHHI GCGC 5 cut(s) 345, 479, 886, 1009, 1019
BstKTI GATC 3 cut(s) 91, 459, 956
BstMBI GATC 3 cut(s) 88, 456, 953
BstMCI CGRYCG 1 cut(s) 499
BstNI CCWGG 1 cut(s) 838
BstNSI RCATGY 1 cut(s) 361
BstSCI CCNGG 3 cut(s) 531, 836, 1287
BstUI CGCG 3 cut(s) 48, 455, 1017
BstV1I GCAGC 4 cut(s) 624, 1056, 1078, 1158
BstZI CGGCCG 1 cut(s) 496
BsuRI GGCC 6 cut(s) 5, 491, 498, 527, 700, 772
BtgI CCRYGG 2 cut(s) 338, 472
BtsCI GGATG 6 cut(s) 390, 516, 760, 1183, 1246, 1270
BtsIMutI CAGTG 2 cut(s) 786, 877
Cac8I GCNNGC 7 cut(s) 53, 328, 496, 529, 766, 770, 1272
CaiI CAGNNNCTG 1 cut(s) 1072
CciI TCATGA 1 cut(s) 862
CfoI GCGC 5 cut(s) 345, 479, 886, 1009, 1019
Cfr10I RCCGGY 2 cut(s) 326, 494
Cfr13I GGNCC 3 cut(s) 594, 698, 854
CpoI CGGWCCG 1 cut(s) 594
Csp6I GTAC 2 cut(s) 895, 981
CspI CGGWCCG 1 cut(s) 594
CviAII CATG 3 cut(s) 358, 863, 1010
CviQI GTAC 2 cut(s) 895, 981
DdeI CTNAG 5 cut(s) 104, 869, 891, 1073, 1298
DinI GGCGCC 2 cut(s) 344, 478
DpnI GATC 3 cut(s) 90, 458, 955
DpnII GATC 3 cut(s) 88, 456, 953
DriI GACNNNNNGTC 1 cut(s) 1131
EaeI YGGCCR 3 cut(s) 3, 496, 770
EagI CGGCCG 1 cut(s) 496
Eam1104I CTCTTC 1 cut(s) 1086
Eam1105I GACNNNNNGTC 1 cut(s) 1131
EarI CTCTTC 1 cut(s) 1086
Ecl136II GAGCTC 2 cut(s) 589, 868
EclXI CGGCCG 1 cut(s) 496
Eco130I CCWWGG 1 cut(s) 929
Eco147I AGGCCT 1 cut(s) 527
Eco24I GRGCYC 3 cut(s) 591, 870, 1060
Eco47I GGWCC 2 cut(s) 594, 854
Eco52I CGGCCG 1 cut(s) 496
Eco53kI GAGCTC 2 cut(s) 589, 868
Eco57I CTGAAG 1 cut(s) 1202
EcoICRI GAGCTC 2 cut(s) 589, 868
EcoRII CCWGG 1 cut(s) 836
EcoT14I CCWWGG 1 cut(s) 929
EcoT38I GRGCYC 3 cut(s) 591, 870, 1060
EgeI GGCGCC 2 cut(s) 344, 478
EheI GGCGCC 2 cut(s) 344, 478
ErhI CCWWGG 1 cut(s) 929
FaeI CATG 3 cut(s) 361, 866, 1013
FaiI YATR 6 cut(s) 359, 602, 864, 899, 948, 1011
FaqI GGGAC 1 cut(s) 1048
FatI CATG 3 cut(s) 357, 862, 1009
FokI GGATG 6 cut(s) 377, 523, 767, 1190, 1253, 1277
FriOI GRGCYC 3 cut(s) 591, 870, 1060
FspBI CTAG 1 cut(s) 191
GlaI GCGC 5 cut(s) 344, 478, 885, 1008, 1018
GsaI CCCAGC 2 cut(s) 618, 698
HaeII RGCGCY 2 cut(s) 346, 480
HaeIII GGCC 6 cut(s) 5, 491, 498, 527, 700, 772
HapII CCGG 9 cut(s) 327, 495, 532, 592, 625, 788, 802, 1175, 1288
HhaI GCGC 5 cut(s) 345, 479, 886, 1009, 1019
Hin1I GRCGYC 2 cut(s) 343, 477
Hin1II CATG 3 cut(s) 361, 866, 1013
Hin6I GCGC 5 cut(s) 343, 477, 884, 1007, 1017
HinP1I GCGC 5 cut(s) 343, 477, 884, 1007, 1017
HinfI GANTC 7 cut(s) 332, 427, 515, 734, 833, 908, 940
HpaII CCGG 9 cut(s) 327, 495, 532, 592, 625, 788, 802, 1175, 1288
HphI GGTGA 4 cut(s) 106, 698, 1063, 1135
Hpy188I TCNGA 5 cut(s) 364, 408, 598, 739, 1152
Hpy188III TCNNGA 4 cut(s) 290, 508, 752, 863
HpyAV CCTTC 9 cut(s) 151, 361, 392, 432, 734, 959, 985, 1081, 1223
HpyCH4III ACNGT 1 cut(s) 781
HpyCH4V TGCA 1 cut(s) 552
HpyF3I CTNAG 5 cut(s) 104, 869, 891, 1073, 1298
Hsp92I GRCGYC 2 cut(s) 343, 477
Hsp92II CATG 3 cut(s) 361, 866, 1013
HspAI GCGC 5 cut(s) 343, 477, 884, 1007, 1017
KasI GGCGCC 2 cut(s) 342, 476
KroI GCCGGC 2 cut(s) 326, 494
KroNI GCCGGC 2 cut(s) 328, 496
Kzo9I GATC 3 cut(s) 88, 456, 953
LmnI GCTCC 3 cut(s) 576, 594, 1210
Lsp1109I GCAGC 4 cut(s) 624, 1056, 1078, 1158
MaeI CTAG 1 cut(s) 191
MaeIII GTNAC 2 cut(s) 112, 1123
MalI GATC 3 cut(s) 90, 458, 955
MbiI CCGCTC 1 cut(s) 85
MboI GATC 3 cut(s) 88, 456, 953
MboII GAAGA 3 cut(s) 70, 1103, 1208
MhlI GDGCHC 4 cut(s) 591, 779, 870, 1060
MluCI AATT 4 cut(s) 569, 609, 643, 1144
Mly113I GGCGCC 2 cut(s) 343, 477
MlyI GAGTC 5 cut(s) 326, 509, 842, 917, 934
MreI CGCCGGCG 1 cut(s) 326
MroNI GCCGGC 2 cut(s) 326, 494
MseI TTAA 1 cut(s) 999
MspA1I CMGCKG 3 cut(s) 15, 1069, 1171
MspI CCGG 9 cut(s) 327, 495, 532, 592, 625, 788, 802, 1175, 1288
MspR9I CCNGG 3 cut(s) 533, 838, 1289
Mva1269I GAATGC 1 cut(s) 299
MvaI CCWGG 1 cut(s) 838
MvnI CGCG 3 cut(s) 48, 455, 1017
NaeI GCCGGC 2 cut(s) 328, 496
NarI GGCGCC 2 cut(s) 343, 477
NciI CCSGG 2 cut(s) 533, 1289
NdeII GATC 3 cut(s) 88, 456, 953
NgoMIV GCCGGC 2 cut(s) 326, 494
NlaIII CATG 3 cut(s) 361, 866, 1013
NlaIV GGNNCC 4 cut(s) 302, 344, 478, 699
NmeAIII GCCGAG 3 cut(s) 547, 798, 983
NmuCI GTSAC 2 cut(s) 112, 1123
NspI RCATGY 1 cut(s) 361
NspV TTCGAA 1 cut(s) 1227
PagI TCATGA 1 cut(s) 862
PceI AGGCCT 1 cut(s) 527
PctI GAATGC 1 cut(s) 299
PdiI GCCGGC 2 cut(s) 328, 496
PfeI GAWTC 2 cut(s) 427, 734
PleI GAGTC 5 cut(s) 326, 509, 841, 916, 934
PluTI GGCGCC 2 cut(s) 346, 480
PpsI GAGTC 5 cut(s) 326, 509, 841, 916, 934
Psp124BI GAGCTC 2 cut(s) 591, 870
Psp6I CCWGG 1 cut(s) 836
PspFI CCCAGC 2 cut(s) 614, 694
PspGI CCWGG 1 cut(s) 836
PspN4I GGNNCC 4 cut(s) 302, 344, 478, 699
PspPI GGNCC 3 cut(s) 594, 698, 854
PstNI CAGNNNCTG 1 cut(s) 1072
PvuII CAGCTG 1 cut(s) 1069
RsaI GTAC 2 cut(s) 896, 982
RsaNI GTAC 2 cut(s) 895, 981
Rsr2I CGGWCCG 1 cut(s) 594
RsrII CGGWCCG 1 cut(s) 594
SacI GAGCTC 2 cut(s) 591, 870
SaqAI TTAA 1 cut(s) 999
Sau3AI GATC 3 cut(s) 88, 456, 953
Sau96I GGNCC 3 cut(s) 594, 698, 854
SchI GAGTC 5 cut(s) 326, 509, 842, 917, 934
ScrFI CCNGG 3 cut(s) 533, 838, 1289
SduI GDGCHC 4 cut(s) 591, 779, 870, 1060
SfoI GGCGCC 2 cut(s) 344, 478
SfuI TTCGAA 1 cut(s) 1227
SgrAI CRCCGGYG 1 cut(s) 326
SinI GGWCC 2 cut(s) 594, 854
SmlI CTYRAG 3 cut(s) 288, 1195, 1282
SmoI CTYRAG 3 cut(s) 288, 1195, 1282
Sse9I AATT 4 cut(s) 569, 609, 643, 1144
SseBI AGGCCT 1 cut(s) 527
SspDI GGCGCC 2 cut(s) 342, 476
SspMI CTAG 1 cut(s) 191
SstI GAGCTC 2 cut(s) 591, 870
StuI AGGCCT 1 cut(s) 527
StyD4I CCNGG 3 cut(s) 531, 836, 1287
StyI CCWWGG 1 cut(s) 929
TaaI ACNGT 1 cut(s) 781
TaqI TCGA 3 cut(s) 87, 425, 1227
TaqII GACCGA 1 cut(s) 547
TasI AATT 4 cut(s) 569, 609, 643, 1144
TatI WGTACW 1 cut(s) 894
TfiI GAWTC 2 cut(s) 427, 734
Tru1I TTAA 1 cut(s) 999
Tru9I TTAA 1 cut(s) 999
TscAI CASTG 2 cut(s) 786, 877
TseFI GTSAC 2 cut(s) 112, 1123
TseI GCWGC 4 cut(s) 637, 1066, 1069, 1171
Tsp45I GTSAC 2 cut(s) 112, 1123
TspDTI ATGAA 2 cut(s) 851, 1169
TspGWI ACGGA 1 cut(s) 933
TspRI CASTG 2 cut(s) 786, 877
VpaK11BI GGWCC 2 cut(s) 594, 854
XceI RCATGY 1 cut(s) 361
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.