pycom15g14570

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009592
Physical Location & Seq
Reverse (-)
9964021 .. 9965352
1332 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g14570.1

Sequence Viewer

Length: 1332 bp
ATGGCTGCCGGCGGCGGAGGCGGCATAGGCGCAGCCAAGAGCACCACCAAAGGCAAGGACAGAAACAAGAAACAAAACCAAACCCACAACAACAACAACAAGCCCCTCAATCAACACCACCTCGGCAGCCTCGTAACCGCCGCCACGTCACTGGCCCACTCCTTCCTCTCCCAGAACGACCTCCTCCTCCTTCCCGCCCAAACCCTAACCCTAGAATCCCTCCTTTCATCCGCCTCCACCTCCCTTTCCACCCTCCTCTGCTTCCCCAACCCACCACCGCAACCACCATTACGCCCTCCGCCGACGCCGCTAGAATGCTGGTTCAGCCGCTTTCTCTCCGCCACCGCCGCCGGCGGAGACTTCGACTCCCACTGGTCCCAGACGTTCCGCATGTCTGAACACTCCTTCTCCGTCCTCCTCTCCCTGCTCTCCCCATTTCTCAAGTCCACAGTCCCTTCAATCCCCCCCAATTTCGTCCTCGCCGCCGCGATTTACCGCTTGGCCCACGGCGCGAGCTACAAGGCTGTGGGAAGGCGGTTCGGGCTCGACTCCATGGAGGCCTGCCGCGCATTTTACGCCGTATGTAAGGCGGTGAACGATCGATTGGGGAACTTGTTCGAATTCCGGTCAGATATATCCCGGGTATCGGCGGCGTTCAGGTGGATTTCGCTGCCTAATTGCTGTGGGGTTTTAGGGTTTTCGAGATTTGGGGTTGGTGGTGAAGTGCTGGGGGCAAATGGGTCATTGTTGGTTCAGGCAGCGGTGGACTCTGAGGGGAGGTTCCTCGATGTCTCCGCCGGGTGGCCGAGCACCATGAAACCCGAATCGATTTTCCGGCAGAGTAAGCTGTATTTGGGTGTGGAGGAGTCCAGGGACTTGCTCAATGGTCCTGCTTTTGAGCTTGGGAATGGGAATTCCATTCCTCAGTACATATTGGGGGACTCTTGCTTCCCTCTATTGCCATGGCTTCTAACACCTTATGTAAGGTCGGACGAGGCGGATAGCTTCAGTTCGATGGAGAAGGCGTTCAATTTGGTGCATACTCGCGCAATGGGGTTGGTTGGCACGGCATTTGGGAGAGTTCGGTCTCGGTGGCAGCTCCTGGCTAGGCAGTGGAAGGAAGAGTGTGTGGAGTTTCTGCCTTTTGTGGTTGTTACAGGGTGTCTGCTGCATAACTTTCTGATCGAGTGTAGCGAGCCGATGCCAGATGACAATGTGAAAGGCTTAAAGGAGGAGGAGCTTCCTGTTTTCGATGGACAAGTGAACGAGAGTGGCGAGCGGATGAGGGATGTACTCGCCATGCACTTGAGCCGGGTAAGCCTCAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

444

Amino Acids

48.46

Weight (kDa)

8.03

Isoelectric Point (pI)

53.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 248 - 392 2.2e-17 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1279
AccII CGCG 4 cut(s) 488, 512, 567, 1047
AcoI YGGCCR 1 cut(s) 803
AcsI RAATTY 2 cut(s) 620, 913
AcuI CTGAAG 1 cut(s) 991
AcyI GRCGYC 1 cut(s) 305
AfaI GTAC 2 cut(s) 929, 1293
AfiI CCNNNNNNNGG 2 cut(s) 646, 801
AgsI TTSAA 2 cut(s) 459, 1030
AjiI CACGTC 1 cut(s) 147
AjnI CCWGG 2 cut(s) 869, 1101
AjuI GAANNNNNNNTTGG 2 cut(s) 587, 619
AloI GAACNNNNNNTCC 2 cut(s) 1010, 1042
AluBI AGCT 6 cut(s) 516, 847, 901, 1005, 1099, 1240
AluI AGCT 6 cut(s) 516, 847, 901, 1005, 1099, 1240
Alw21I GWGCWC 2 cut(s) 44, 812
Alw26I GTCTC 3 cut(s) 351, 796, 1092
AlwNI CAGNNNCTG 1 cut(s) 1102
Ama87I CYCGRG 1 cut(s) 639
AoxI GGCC 4 cut(s) 153, 501, 558, 803
ApeKI GCWGC 7 cut(s) 5, 32, 126, 670, 758, 1096, 1168
ApoI RAATTY 2 cut(s) 620, 913
Asp700I GAANNNNTTC 2 cut(s) 614, 1025
AspLEI GCGC 4 cut(s) 32, 512, 569, 1049
AspS9I GGNCC 4 cut(s) 154, 375, 502, 887
AsuC2I CCSGG 4 cut(s) 640, 641, 799, 1313
AsuHPI GGTGA 2 cut(s) 604, 731
AsuII TTCGAA 1 cut(s) 618
AvaI CYCGRG 1 cut(s) 639
AvaII GGWCC 2 cut(s) 375, 887
BanII GRGCYC 1 cut(s) 546
Bbv12I GWGCWC 2 cut(s) 44, 812
BbvI GCAGC 6 cut(s) 44, 138, 657, 770, 1108, 1155
BccI CCATC 2 cut(s) 1009, 1247
BceAI ACGGC 3 cut(s) 523, 563, 1083
BciT130I CCWGG 2 cut(s) 871, 1103
BcnI CCSGG 4 cut(s) 640, 641, 799, 1313
BcoDI GTCTC 3 cut(s) 351, 796, 1092
BfaI CTAG 3 cut(s) 212, 311, 1107
Bme1390I CCNGG 6 cut(s) 640, 641, 799, 871, 1103, 1313
Bme18I GGWCC 2 cut(s) 375, 887
BmeT110I CYCGRG 1 cut(s) 639
BmgBI CACGTC 1 cut(s) 147
BmgT120I GGNCC 4 cut(s) 154, 375, 502, 887
BmiI GGNNCC 2 cut(s) 377, 782
BmrFI CCNGG 6 cut(s) 640, 641, 799, 871, 1103, 1313
BmsI GCATC 1 cut(s) 1191
Bpu14I TTCGAA 1 cut(s) 618
BpuEI CTTGAG 2 cut(s) 425, 1327
BpuMI CCSGG 4 cut(s) 640, 641, 799, 1313
Bsa29I ATCGAT 2 cut(s) 601, 827
BsaHI GRCGYC 1 cut(s) 305
BsaI GGTCTC 1 cut(s) 1092
BsaJI CCNNGG 6 cut(s) 121, 505, 552, 639, 870, 962
BsaWI WCCGGW 1 cut(s) 624
BsaXI ACNNNNNCTCC 6 cut(s) 350, 380, 392, 422, 1010, 1040
Bsc4I CCNNNNNNNGG 2 cut(s) 646, 801
Bse118I RCCGGY 2 cut(s) 8, 350
Bse1I ACTGG 2 cut(s) 156, 377
Bse3DI GCAATG 1 cut(s) 1056
BseBI CCWGG 2 cut(s) 871, 1103
BseCI ATCGAT 2 cut(s) 601, 827
BseDI CCNNGG 6 cut(s) 121, 505, 552, 639, 870, 962
BseGI GGATG 3 cut(s) 227, 1287, 1294
BseLI CCNNNNNNNGG 2 cut(s) 646, 801
BseMI GCAATG 1 cut(s) 1056
BseMII CTCAG 2 cut(s) 762, 938
BseNI ACTGG 2 cut(s) 156, 377
BseRI GAGGAG 7 cut(s) 173, 176, 245, 407, 878, 1247, 1250
BseXI GCAGC 6 cut(s) 44, 138, 657, 770, 1108, 1155
BseYI CCCAGC 1 cut(s) 727
Bsh1236I CGCG 4 cut(s) 488, 512, 567, 1047
Bsh1285I CGRYCG 1 cut(s) 601
BshFI GGCC 4 cut(s) 155, 503, 560, 805
BshVI ATCGAT 2 cut(s) 601, 827
BsiEI CGRYCG 1 cut(s) 601
BsiHKAI GWGCWC 2 cut(s) 44, 812
BsiHKCI CYCGRG 1 cut(s) 639
BsiSI CCGG 7 cut(s) 9, 351, 625, 640, 798, 835, 1312
BslFI GGGAC 4 cut(s) 361, 437, 887, 953
BslI CCNNNNNNNGG 2 cut(s) 646, 801
BsmAI GTCTC 3 cut(s) 351, 796, 1092
BsmFI GGGAC 4 cut(s) 361, 437, 887, 953
BsmI GAATGC 1 cut(s) 320
BsnI GGCC 4 cut(s) 155, 503, 560, 805
Bso31I GGTCTC 1 cut(s) 1092
BsoBI CYCGRG 1 cut(s) 639
Bsp119I TTCGAA 1 cut(s) 618
Bsp1286I GDGCHC 3 cut(s) 44, 546, 812
Bsp143I GATC 2 cut(s) 598, 1182
Bsp19I CCATGG 2 cut(s) 552, 962
BspANI GGCC 4 cut(s) 155, 503, 560, 805
BspCNI CTCAG 2 cut(s) 763, 937
BspDI ATCGAT 2 cut(s) 601, 827
BspFNI CGCG 4 cut(s) 488, 512, 567, 1047
BspLI GGNNCC 2 cut(s) 377, 782
BspT104I TTCGAA 1 cut(s) 618
BspTNI GGTCTC 1 cut(s) 1092
BsrBI CCGCTC 1 cut(s) 1279
BsrDI GCAATG 1 cut(s) 1056
BsrFI RCCGGY 2 cut(s) 8, 350
BsrI ACTGG 2 cut(s) 156, 377
BssAI RCCGGY 2 cut(s) 8, 350
BssECI CCNNGG 6 cut(s) 121, 505, 552, 639, 870, 962
BssMI GATC 2 cut(s) 598, 1182
BssNI GRCGYC 1 cut(s) 305
BssT1I CCWWGG 2 cut(s) 552, 962
Bst2UI CCWGG 2 cut(s) 871, 1103
Bst4CI ACNGT 1 cut(s) 451
Bst6I CTCTTC 1 cut(s) 1116
BstACI GRCGYC 1 cut(s) 305
BstBI TTCGAA 1 cut(s) 618
BstC8I GCNNGC 6 cut(s) 10, 352, 514, 562, 1196, 1277
BstDEI CTNAG 3 cut(s) 771, 924, 1322
BstDSI CCRYGG 3 cut(s) 505, 552, 962
BstF5I GGATG 3 cut(s) 227, 1287, 1294
BstFNI CGCG 4 cut(s) 488, 512, 567, 1047
BstHHI GCGC 4 cut(s) 32, 512, 569, 1049
BstKTI GATC 2 cut(s) 601, 1185
BstMAI GTCTC 3 cut(s) 351, 796, 1092
BstMBI GATC 2 cut(s) 598, 1182
BstMCI CGRYCG 1 cut(s) 601
BstNI CCWGG 2 cut(s) 871, 1103
BstNSI RCATGY 1 cut(s) 394
BstSCI CCNGG 6 cut(s) 638, 639, 797, 869, 1101, 1311
BstUI CGCG 4 cut(s) 488, 512, 567, 1047
BstV1I GCAGC 6 cut(s) 44, 138, 657, 770, 1108, 1155
BstXI CCANNNNNNTGG 1 cut(s) 151
Bsu15I ATCGAT 2 cut(s) 601, 827
BsuRI GGCC 4 cut(s) 155, 503, 560, 805
BsuTUI ATCGAT 2 cut(s) 601, 827
BtgI CCRYGG 3 cut(s) 505, 552, 962
BtrI CACGTC 1 cut(s) 147
BtsCI GGATG 3 cut(s) 227, 1287, 1294
BtsI GCAGTG 1 cut(s) 1118
BtsIMutI CAGTG 3 cut(s) 149, 370, 1118
Cac8I GCNNGC 6 cut(s) 10, 352, 514, 562, 1196, 1277
CaiI CAGNNNCTG 1 cut(s) 1102
CfoI GCGC 4 cut(s) 32, 512, 569, 1049
Cfr10I RCCGGY 2 cut(s) 8, 350
Cfr13I GGNCC 4 cut(s) 154, 375, 502, 887
Cfr9I CCCGGG 1 cut(s) 639
ClaI ATCGAT 2 cut(s) 601, 827
CseI GACGC 1 cut(s) 313
Csp6I GTAC 2 cut(s) 928, 1292
CviAII CATG 5 cut(s) 391, 553, 814, 963, 1300
CviQI GTAC 2 cut(s) 928, 1292
DdeI CTNAG 3 cut(s) 771, 924, 1322
DpnI GATC 2 cut(s) 600, 1184
DpnII GATC 2 cut(s) 598, 1182
EaeI YGGCCR 1 cut(s) 803
Eam1104I CTCTTC 1 cut(s) 1116
EarI CTCTTC 1 cut(s) 1116
EciI GGCGGA 7 cut(s) 30, 220, 288, 328, 369, 784, 1013
Eco130I CCWWGG 2 cut(s) 552, 962
Eco147I AGGCCT 1 cut(s) 560
Eco24I GRGCYC 1 cut(s) 546
Eco31I GGTCTC 1 cut(s) 1092
Eco47I GGWCC 2 cut(s) 375, 887
Eco57I CTGAAG 1 cut(s) 991
Eco88I CYCGRG 1 cut(s) 639
EcoRI GAATTC 2 cut(s) 620, 913
EcoRII CCWGG 2 cut(s) 869, 1101
EcoT14I CCWWGG 2 cut(s) 552, 962
EcoT38I GRGCYC 1 cut(s) 546
ErhI CCWWGG 2 cut(s) 552, 962
FaeI CATG 5 cut(s) 394, 556, 817, 966, 1303
FaqI GGGAC 4 cut(s) 361, 437, 887, 953
FatI CATG 5 cut(s) 390, 552, 813, 962, 1299
FauI CCCGC 1 cut(s) 202
FokI GGATG 3 cut(s) 214, 1294, 1301
FriOI GRGCYC 1 cut(s) 546
FspBI CTAG 3 cut(s) 212, 311, 1107
GlaI GCGC 4 cut(s) 31, 511, 568, 1048
GsaI CCCAGC 1 cut(s) 731
HaeIII GGCC 4 cut(s) 155, 503, 560, 805
HapII CCGG 7 cut(s) 9, 351, 625, 640, 798, 835, 1312
HgaI GACGC 1 cut(s) 313
HhaI GCGC 4 cut(s) 32, 512, 569, 1049
Hin1I GRCGYC 1 cut(s) 305
Hin1II CATG 5 cut(s) 394, 556, 817, 966, 1303
Hin6I GCGC 4 cut(s) 30, 510, 567, 1047
HinP1I GCGC 4 cut(s) 30, 510, 567, 1047
HinfI GANTC 7 cut(s) 215, 365, 548, 767, 824, 866, 941
HpaII CCGG 7 cut(s) 9, 351, 625, 640, 798, 835, 1312
HphI GGTGA 2 cut(s) 604, 731
Hpy166II GTNNAC 4 cut(s) 447, 595, 766, 1264
Hpy188I TCNGA 6 cut(s) 397, 631, 772, 991, 1182, 1325
Hpy188III TCNNGA 1 cut(s) 702
Hpy8I GTNNAC 4 cut(s) 447, 595, 766, 1264
Hpy99I CGWCG 1 cut(s) 307
HpyAV CCTTC 7 cut(s) 172, 200, 415, 465, 525, 1015, 1111
HpyCH4III ACNGT 1 cut(s) 451
HpyCH4IV ACGT 2 cut(s) 146, 383
HpyCH4V TGCA 3 cut(s) 1039, 1171, 1303
HpyF3I CTNAG 3 cut(s) 771, 924, 1322
HpySE526I ACGT 2 cut(s) 146, 383
Hsp92I GRCGYC 1 cut(s) 305
Hsp92II CATG 5 cut(s) 394, 556, 817, 966, 1303
HspAI GCGC 4 cut(s) 30, 510, 567, 1047
KroI GCCGGC 2 cut(s) 8, 350
KroNI GCCGGC 2 cut(s) 10, 352
Kzo9I GATC 2 cut(s) 598, 1182
LmnI GCTCC 2 cut(s) 1104, 1237
Lsp1109I GCAGC 6 cut(s) 44, 138, 657, 770, 1108, 1155
LweI GCATC 1 cut(s) 1191
MaeI CTAG 3 cut(s) 212, 311, 1107
MaeII ACGT 2 cut(s) 146, 383
MaeIII GTNAC 3 cut(s) 133, 147, 1153
MalI GATC 2 cut(s) 600, 1184
MbiI CCGCTC 1 cut(s) 1279
MboI GATC 2 cut(s) 598, 1182
MboII GAAGA 1 cut(s) 1133
MhlI GDGCHC 3 cut(s) 44, 546, 812
MluCI AATT 5 cut(s) 469, 620, 676, 913, 1030
MlyI GAGTC 5 cut(s) 359, 542, 761, 875, 935
MmeI TCCRAC 1 cut(s) 969
MreI CGCCGGCG 1 cut(s) 350
MroNI GCCGGC 2 cut(s) 8, 350
MroXI GAANNNNTTC 2 cut(s) 614, 1025
MseI TTAA 1 cut(s) 1226
MspA1I CMGCKG 1 cut(s) 761
MspI CCGG 7 cut(s) 9, 351, 625, 640, 798, 835, 1312
MspR9I CCNGG 6 cut(s) 640, 641, 799, 871, 1103, 1313
Mva1269I GAATGC 1 cut(s) 320
MvaI CCWGG 2 cut(s) 871, 1103
MvnI CGCG 4 cut(s) 488, 512, 567, 1047
NaeI GCCGGC 2 cut(s) 10, 352
NciI CCSGG 4 cut(s) 640, 641, 799, 1313
NcoI CCATGG 2 cut(s) 552, 962
NdeII GATC 2 cut(s) 598, 1182
NgoMIV GCCGGC 2 cut(s) 8, 350
NlaIII CATG 5 cut(s) 394, 556, 817, 966, 1303
NlaIV GGNNCC 2 cut(s) 377, 782
NmeAIII GCCGAG 2 cut(s) 102, 831
NmuCI GTSAC 1 cut(s) 147
NspI RCATGY 1 cut(s) 394
NspV TTCGAA 1 cut(s) 618
PceI AGGCCT 1 cut(s) 560
PcsI WCGNNNNNNNCGW 3 cut(s) 129, 1191, 1272
PctI GAATGC 1 cut(s) 320
PdiI GCCGGC 2 cut(s) 10, 352
PdmI GAANNNNTTC 2 cut(s) 614, 1025
PfeI GAWTC 2 cut(s) 215, 824
Ple19I CGATCG 1 cut(s) 601
PleI GAGTC 5 cut(s) 359, 542, 761, 874, 935
PpsI GAGTC 5 cut(s) 359, 542, 761, 874, 935
Psp6I CCWGG 2 cut(s) 869, 1101
PspFI CCCAGC 1 cut(s) 727
PspGI CCWGG 2 cut(s) 869, 1101
PspN4I GGNNCC 2 cut(s) 377, 782
PspPI GGNCC 4 cut(s) 154, 375, 502, 887
PstNI CAGNNNCTG 1 cut(s) 1102
PvuI CGATCG 1 cut(s) 601
RsaI GTAC 2 cut(s) 929, 1293
RsaNI GTAC 2 cut(s) 928, 1292
SaqAI TTAA 1 cut(s) 1226
Sau3AI GATC 2 cut(s) 598, 1182
Sau96I GGNCC 4 cut(s) 154, 375, 502, 887
SchI GAGTC 5 cut(s) 359, 542, 761, 875, 935
ScrFI CCNGG 6 cut(s) 640, 641, 799, 871, 1103, 1313
SduI GDGCHC 3 cut(s) 44, 546, 812
SfaNI GCATC 1 cut(s) 1191
SfuI TTCGAA 1 cut(s) 618
SgrAI CRCCGGYG 1 cut(s) 350
SinI GGWCC 2 cut(s) 375, 887
SmaI CCCGGG 1 cut(s) 641
SmlI CTYRAG 2 cut(s) 440, 1306
SmoI CTYRAG 2 cut(s) 440, 1306
Sse9I AATT 5 cut(s) 469, 620, 676, 913, 1030
SseBI AGGCCT 1 cut(s) 560
SspMI CTAG 3 cut(s) 212, 311, 1107
StuI AGGCCT 1 cut(s) 560
StyD4I CCNGG 6 cut(s) 638, 639, 797, 869, 1101, 1311
StyI CCWWGG 2 cut(s) 552, 962
TaaI ACNGT 1 cut(s) 451
TaiI ACGT 2 cut(s) 149, 386
TaqII GACCGA 1 cut(s) 1074
TasI AATT 5 cut(s) 469, 620, 676, 913, 1030
TatI WGTACW 2 cut(s) 927, 1291
TfiI GAWTC 2 cut(s) 215, 824
Tru1I TTAA 1 cut(s) 1226
Tru9I TTAA 1 cut(s) 1226
TscAI CASTG 3 cut(s) 156, 377, 1118
TseFI GTSAC 1 cut(s) 147
TseI GCWGC 7 cut(s) 5, 32, 126, 670, 758, 1096, 1168
Tsp45I GTSAC 1 cut(s) 147
TspDTI ATGAA 2 cut(s) 216, 830
TspGWI ACGGA 1 cut(s) 400
TspMI CCCGGG 1 cut(s) 639
TspRI CASTG 3 cut(s) 156, 377, 1118
VpaK11BI GGWCC 2 cut(s) 375, 887
XapI RAATTY 2 cut(s) 620, 913
XceI RCATGY 1 cut(s) 394
XmaI CCCGGG 1 cut(s) 639
XmnI GAANNNNTTC 2 cut(s) 614, 1025
XspI CTAG 3 cut(s) 212, 311, 1107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.