RLG00000002276

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0009592
Physical Location & Seq
Forward (+)
31207579 .. 31208434
856 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002276

Sequence Viewer

Length: 750 bp
ATGTCATTCTCTCCCTTCAATTCCGCCCAATTACACCCTCGCTGCCGCGATTTACCACATCGCCTATGGTGCCAGCTACAAGGCGGTCGGCCGGCGCTTCGGGATGGGCTCGGCGGAGGCCTGCCGGGCTTTCTACTCCGTCTGCAAGGTCTGGGAGATTTGGGGTGGCTGGTGAAATGTTGGGGGCCAAATGGGTCGTTTTTGGTTCAGGCATTGGTGGATTCCGAGGGGAGGTTTCTGGATGTTTCGGCTGGTTGGCCGAGCACAGTGAAGCCGGAAGCTATTTTCCGGCAGGGTAAGCTGTATTTGGGTGTGGAGGAGTCTAGGGAGTTGTTAAATGGTCCTGTTCATGAGCTTAGTGATGGCAATGTGCTTCCTCAGTACATGTTGGGGGAGTCTTGCTTCCCTCTGTTGCCTTGGCTTTTAACTCCGTGTGTAAGATCAAATGAGGAAGAGCAGAGTTTTGGTACATTGGAGAAGGTGTCTAACTCGGCGCATAGTCGTGCAATGGGGCTGGTTGGGACGGCATTTGCCAGGGTGAGGGCTCGGTGGCAGCTGCTGAGTAGGGAGTGGAAGGAAGAGTGTATGGAGTTTTTGCTGTCTGTGGTGGTTACTGGTTGTCTGCTGAATAATTTTCTGATGAAGTGTAGTGAGCCGCTGCTGGATGACAATGTGGGGTGCTTGAGGGAGGAGGAGCTTCAGGTGTTTGAAGGACAGATGGATGAGAGTGGCGAGAGCCGAGAGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

250

Amino Acids

27.78

Weight (kDa)

4.99

Isoelectric Point (pI)

56.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 66 - 211 3.3e-12 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 69
AccII CGCG 1 cut(s) 48
AciI CCGC 5 cut(s) 24, 46, 84, 114, 656
AcoI YGGCCR 2 cut(s) 89, 257
AcuI CTGAAG 1 cut(s) 683
AfaI GTAC 2 cut(s) 383, 469
AfiI CCNNNNNNNGG 2 cut(s) 231, 540
AflIII ACRYGT 1 cut(s) 384
AgsI TTSAA 2 cut(s) 19, 710
AjnI CCWGG 1 cut(s) 533
AluBI AGCT 6 cut(s) 76, 281, 301, 355, 556, 697
AluI AGCT 6 cut(s) 76, 281, 301, 355, 556, 697
Alw21I GWGCWC 1 cut(s) 266
AlwNI CAGNNNCTG 1 cut(s) 559
AoxI GGCC 4 cut(s) 89, 118, 185, 257
ApeKI GCWGC 4 cut(s) 42, 553, 556, 658
AspLEI GCGC 2 cut(s) 97, 496
AspS9I GGNCC 2 cut(s) 185, 341
AsuC2I CCSGG 1 cut(s) 126
AsuHPI GGTGA 2 cut(s) 184, 550
AvaII GGWCC 1 cut(s) 341
BanI GGYRCC 1 cut(s) 69
BanII GRGCYC 2 cut(s) 111, 547
Bbv12I GWGCWC 1 cut(s) 266
BbvI GCAGC 4 cut(s) 29, 543, 565, 645
BccI CCATC 3 cut(s) 98, 356, 712
BceAI ACGGC 1 cut(s) 540
BciT130I CCWGG 1 cut(s) 535
BcnI CCSGG 1 cut(s) 126
BfaI CTAG 1 cut(s) 324
BfoI RGCGCY 1 cut(s) 98
BglI GCCNNNNNGGC 1 cut(s) 126
BisI GCNGC 6 cut(s) 43, 46, 554, 557, 656, 659
BlsI GCNGC 6 cut(s) 44, 47, 555, 558, 657, 660
Bme1390I CCNGG 2 cut(s) 126, 535
Bme18I GGWCC 1 cut(s) 341
BmgT120I GGNCC 2 cut(s) 185, 341
BmiI GGNNCC 2 cut(s) 71, 186
BmrFI CCNGG 2 cut(s) 126, 535
BpuEI CTTGAG 1 cut(s) 703
BpuMI CCSGG 1 cut(s) 126
BsaJI CCNNGG 3 cut(s) 225, 416, 534
BsaXI ACNNNNNCTCC 2 cut(s) 467, 497
Bsc4I CCNNNNNNNGG 2 cut(s) 231, 540
Bse118I RCCGGY 1 cut(s) 91
Bse1I ACTGG 1 cut(s) 619
Bse3DI GCAATG 2 cut(s) 373, 513
BseBI CCWGG 1 cut(s) 535
BseDI CCNNGG 3 cut(s) 225, 416, 534
BseGI GGATG 4 cut(s) 109, 247, 670, 727
BseLI CCNNNNNNNGG 2 cut(s) 231, 540
BseMI GCAATG 2 cut(s) 373, 513
BseMII CTCAG 2 cut(s) 392, 551
BseNI ACTGG 1 cut(s) 619
BseRI GAGGAG 3 cut(s) 332, 704, 707
BseX3I CGGCCG 1 cut(s) 89
BseXI GCAGC 4 cut(s) 29, 543, 565, 645
Bsh1236I CGCG 1 cut(s) 48
Bsh1285I CGRYCG 2 cut(s) 88, 92
BshFI GGCC 4 cut(s) 91, 120, 187, 259
BshNI GGYRCC 1 cut(s) 69
BsiEI CGRYCG 2 cut(s) 88, 92
BsiHKAI GWGCWC 1 cut(s) 266
BsiSI CCGG 4 cut(s) 92, 125, 275, 289
BslFI GGGAC 1 cut(s) 535
BslI CCNNNNNNNGG 2 cut(s) 231, 540
BsmFI GGGAC 1 cut(s) 535
BsnI GGCC 4 cut(s) 91, 120, 187, 259
Bsp1286I GDGCHC 3 cut(s) 111, 266, 547
Bsp143I GATC 1 cut(s) 440
BspACI CCGC 5 cut(s) 24, 46, 84, 114, 656
BspANI GGCC 4 cut(s) 91, 120, 187, 259
BspCNI CTCAG 2 cut(s) 391, 552
BspFNI CGCG 1 cut(s) 48
BspHI TCATGA 1 cut(s) 349
BspLI GGNNCC 2 cut(s) 71, 186
BspQI GCTCTTC 1 cut(s) 447
BspT107I GGYRCC 1 cut(s) 69
BsrDI GCAATG 2 cut(s) 373, 513
BsrFI RCCGGY 1 cut(s) 91
BsrI ACTGG 1 cut(s) 619
BssAI RCCGGY 1 cut(s) 91
BssECI CCNNGG 3 cut(s) 225, 416, 534
BssMI GATC 1 cut(s) 440
BssT1I CCWWGG 1 cut(s) 416
Bst2UI CCWGG 1 cut(s) 535
Bst4CI ACNGT 1 cut(s) 268
Bst6I CTCTTC 2 cut(s) 447, 573
BstC8I GCNNGC 3 cut(s) 74, 93, 122
BstDEI CTNAG 3 cut(s) 356, 378, 560
BstF5I GGATG 4 cut(s) 109, 247, 670, 727
BstFNI CGCG 1 cut(s) 48
BstH2I RGCGCY 1 cut(s) 98
BstHHI GCGC 2 cut(s) 97, 496
BstKTI GATC 1 cut(s) 443
BstMBI GATC 1 cut(s) 440
BstMCI CGRYCG 2 cut(s) 88, 92
BstMWI GCNNNNNNNGC 3 cut(s) 69, 126, 298
BstNI CCWGG 1 cut(s) 535
BstNSI RCATGY 1 cut(s) 388
BstSCI CCNGG 2 cut(s) 124, 533
BstUI CGCG 1 cut(s) 48
BstV1I GCAGC 4 cut(s) 29, 543, 565, 645
BstZI CGGCCG 1 cut(s) 89
BsuRI GGCC 4 cut(s) 91, 120, 187, 259
BtgZI GCGATG 1 cut(s) 44
BtsCI GGATG 4 cut(s) 109, 247, 670, 727
BtsIMutI CAGTG 1 cut(s) 273
Cac8I GCNNGC 3 cut(s) 74, 93, 122
CaiI CAGNNNCTG 1 cut(s) 559
CciI TCATGA 1 cut(s) 349
CfoI GCGC 2 cut(s) 97, 496
Cfr10I RCCGGY 1 cut(s) 91
Cfr13I GGNCC 2 cut(s) 185, 341
Csp6I GTAC 2 cut(s) 382, 468
CviAII CATG 2 cut(s) 350, 385
CviQI GTAC 2 cut(s) 382, 468
DdeI CTNAG 3 cut(s) 356, 378, 560
DpnI GATC 1 cut(s) 442
DpnII GATC 1 cut(s) 440
EaeI YGGCCR 2 cut(s) 89, 257
EagI CGGCCG 1 cut(s) 89
Eam1104I CTCTTC 2 cut(s) 447, 573
EarI CTCTTC 2 cut(s) 447, 573
EciI GGCGGA 2 cut(s) 13, 129
EclXI CGGCCG 1 cut(s) 89
Eco130I CCWWGG 1 cut(s) 416
Eco147I AGGCCT 1 cut(s) 120
Eco24I GRGCYC 2 cut(s) 111, 547
Eco47I GGWCC 1 cut(s) 341
Eco52I CGGCCG 1 cut(s) 89
Eco57I CTGAAG 1 cut(s) 683
EcoRII CCWGG 1 cut(s) 533
EcoT14I CCWWGG 1 cut(s) 416
EcoT38I GRGCYC 2 cut(s) 111, 547
ErhI CCWWGG 1 cut(s) 416
FaeI CATG 2 cut(s) 353, 388
FaiI YATR 5 cut(s) 67, 351, 386, 498, 587
FaqI GGGAC 1 cut(s) 535
FatI CATG 2 cut(s) 349, 384
Fnu4HI GCNGC 6 cut(s) 43, 46, 554, 557, 656, 659
FokI GGATG 4 cut(s) 116, 254, 677, 734
FriOI GRGCYC 2 cut(s) 111, 547
Fsp4HI GCNGC 6 cut(s) 43, 46, 554, 557, 656, 659
FspBI CTAG 1 cut(s) 324
GlaI GCGC 2 cut(s) 96, 495
GluI GCNGC 6 cut(s) 43, 46, 554, 557, 656, 659
HaeII RGCGCY 1 cut(s) 98
HaeIII GGCC 4 cut(s) 91, 120, 187, 259
HapII CCGG 4 cut(s) 92, 125, 275, 289
HhaI GCGC 2 cut(s) 97, 496
Hin1II CATG 2 cut(s) 353, 388
Hin6I GCGC 2 cut(s) 95, 494
HinP1I GCGC 2 cut(s) 95, 494
HinfI GANTC 3 cut(s) 221, 320, 395
HpaII CCGG 4 cut(s) 92, 125, 275, 289
HphI GGTGA 2 cut(s) 184, 550
Hpy188I TCNGA 2 cut(s) 226, 639
Hpy188III TCNNGA 3 cut(s) 101, 239, 350
HpyAV CCTTC 4 cut(s) 25, 472, 568, 704
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4V TGCA 2 cut(s) 145, 506
HpyF10VI GCNNNNNNNGC 3 cut(s) 69, 126, 298
HpyF3I CTNAG 3 cut(s) 356, 378, 560
Hsp92II CATG 2 cut(s) 353, 388
HspAI GCGC 2 cut(s) 95, 494
KroI GCCGGC 1 cut(s) 91
KroNI GCCGGC 1 cut(s) 93
Kzo9I GATC 1 cut(s) 440
LguI GCTCTTC 1 cut(s) 447
LmnI GCTCC 1 cut(s) 694
Lsp1109I GCAGC 4 cut(s) 29, 543, 565, 645
MaeI CTAG 1 cut(s) 324
MaeIII GTNAC 1 cut(s) 610
MalI GATC 1 cut(s) 442
MboI GATC 1 cut(s) 440
MboII GAAGA 2 cut(s) 464, 590
MhlI GDGCHC 3 cut(s) 111, 266, 547
MluCI AATT 3 cut(s) 19, 29, 631
MlyI GAGTC 2 cut(s) 329, 404
MroNI GCCGGC 1 cut(s) 91
MseI TTAA 2 cut(s) 335, 425
MslI CAYNNNNRTG 1 cut(s) 501
MspA1I CMGCKG 2 cut(s) 556, 658
MspI CCGG 4 cut(s) 92, 125, 275, 289
MspR9I CCNGG 2 cut(s) 126, 535
MvaI CCWGG 1 cut(s) 535
MvnI CGCG 1 cut(s) 48
MwoI GCNNNNNNNGC 3 cut(s) 69, 126, 298
NaeI GCCGGC 1 cut(s) 93
NciI CCSGG 1 cut(s) 126
NdeII GATC 1 cut(s) 440
NgoMIV GCCGGC 1 cut(s) 91
NlaIII CATG 2 cut(s) 353, 388
NlaIV GGNNCC 2 cut(s) 71, 186
NmeAIII GCCGAG 3 cut(s) 90, 285, 470
NspI RCATGY 1 cut(s) 388
PagI TCATGA 1 cut(s) 349
PceI AGGCCT 1 cut(s) 120
PciI ACATGT 1 cut(s) 384
PciSI GCTCTTC 1 cut(s) 447
PdiI GCCGGC 1 cut(s) 93
PfeI GAWTC 1 cut(s) 221
PkrI GCNGC 6 cut(s) 44, 47, 555, 558, 657, 660
PleI GAGTC 2 cut(s) 328, 403
PpsI GAGTC 2 cut(s) 328, 403
PscI ACATGT 1 cut(s) 384
Psp6I CCWGG 1 cut(s) 533
PspGI CCWGG 1 cut(s) 533
PspN4I GGNNCC 2 cut(s) 71, 186
PspPI GGNCC 2 cut(s) 185, 341
PstNI CAGNNNCTG 1 cut(s) 559
PvuII CAGCTG 1 cut(s) 556
RsaI GTAC 2 cut(s) 383, 469
RsaNI GTAC 2 cut(s) 382, 468
RseI CAYNNNNRTG 1 cut(s) 501
SapI GCTCTTC 1 cut(s) 447
SaqAI TTAA 2 cut(s) 335, 425
SatI GCNGC 6 cut(s) 43, 46, 554, 557, 656, 659
Sau3AI GATC 1 cut(s) 440
Sau96I GGNCC 2 cut(s) 185, 341
SchI GAGTC 2 cut(s) 329, 404
ScrFI CCNGG 2 cut(s) 126, 535
SduI GDGCHC 3 cut(s) 111, 266, 547
SinI GGWCC 1 cut(s) 341
SmiMI CAYNNNNRTG 1 cut(s) 501
SmlI CTYRAG 1 cut(s) 682
SmoI CTYRAG 1 cut(s) 682
Sse9I AATT 3 cut(s) 19, 29, 631
SseBI AGGCCT 1 cut(s) 120
SsiI CCGC 5 cut(s) 24, 46, 84, 114, 656
SspMI CTAG 1 cut(s) 324
StuI AGGCCT 1 cut(s) 120
StyD4I CCNGG 2 cut(s) 124, 533
StyI CCWWGG 1 cut(s) 416
TaaI ACNGT 1 cut(s) 268
TasI AATT 3 cut(s) 19, 29, 631
TatI WGTACW 1 cut(s) 381
TauI GCSGC 2 cut(s) 48, 658
TfiI GAWTC 1 cut(s) 221
Tru1I TTAA 2 cut(s) 335, 425
Tru9I TTAA 2 cut(s) 335, 425
TscAI CASTG 1 cut(s) 273
TseI GCWGC 4 cut(s) 42, 553, 556, 658
TspDTI ATGAA 2 cut(s) 338, 656
TspGWI ACGGA 2 cut(s) 128, 420
TspRI CASTG 1 cut(s) 273
VpaK11BI GGWCC 1 cut(s) 341
XceI RCATGY 1 cut(s) 388
XcmI CCANNNNNNNNNTGG 1 cut(s) 63
XspI CTAG 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.