Rw2G001920

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0009592
Physical Location & Seq
Reverse (-)
1579080 .. 1580635
1556 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G001920.1

Sequence Viewer

Length: 1305 bp
ATGTCCGCCGTCAGCGGCGGCGGAGCAGCCAAGAACACCGCCAAGCGCGGCAAGCGGAAGAACAAGAACCACCACACTCCGCCGCTCGATCAACGAAACCTCCTCAGCCTCGTCACCTCCGCCACCTCACTCGCCCACTCCTTCCTCTCCCAAAACGACCTCCACCTCCTCCCCTCCCAAACCCTAACCCTAGAGACCCTCCTCTCCTCCGCCTCCTCCGCCCTCTCCCCCCTCCTCCTCCTCCCATCTCCTCCTCCGCCTCCGCCGCCGCAACCGCCGCCGCCTCCTCCTCAAGAATGCTGGTTCCGCCGCTTCCTCTCCTCCGCCCCCGGCGACTCGCCGTGGCGCCACGCTTTCCGCATGTCCGAACACTCGTTCTCTATCCTTCTCTCCATCCTCTCCCCGTTTCTGACCTCCGCAATCCCTTCGATTCCGCCCAACTACGCCCTCGCCGCCGCGATTTACCGCCTCGCCCACTGCGCCACCTACAAGGCGGTCGGCCGGCGCTTCGGGATGGACTCGGCGGAGGCCTGCCGGGCTTTCTACTCCGTCTGCAAGTCGGTCAATGATAAATTAGGGACCTTGTTTGAGCTCCGGTCCGATATGGCTCGGATTGTTGGGGGTTTCGGGTGGATTTCGCTGCCGAATTGCTGTGGGGTGTTGGGGTTTGGGAGATTTGGGGTTAGTGGTGAAATGCTGGGGCCAAATGGGTCGCTGTTGGTTCAGGCATTGGTGGATTCTGAAGGGAGGTTTCTGGATGTTTCGGCTGGCTGGCCGAGCACAGTGAAACCGGAAGCGATTTTCCGGCAGAGTAAGCTGTATTTGGGTGTGGAGGAGTCCGGGGAGTTGTTGAATGGTCCTGTTTATGAGCTCAGTGATGGCAATGCGCTTCCTCAGTACATGCTGGGGGACTCTTGCTTCCCTCTGTTGCCTTGGCTTTTGACTCCGTATGTGAGATCAAATGAGGAAGAGGAGAGTTTCGGTACATTGGAGAAGGCGTTTAACTCAGCGCATGGTCGCGCAATGGGGCTGGTTGGGACGGCGTTTGCGAGGGTGAGGGCTCGGTGGCAGCTGCTGAGTAGGGAGTGGAAGGAAGAGTGTGTGGAGTTTTTGCCGTTTGTGGTGGTTACTGGCTGTCTGCTGAATAATTTTCTGATGAAGTGTAGTGAGCCGCTGCCGGATGACAATGTGGGGTGCTTGAGGGAGGAGGAGCTTCAGGTGTTCGAAGGACAGGTGGATGAGAGTGGCGAGAGGATTAGGGATGTGCTTGCCACTTACTTGAGCCGGGTAAGCCTAAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

434

Amino Acids

47.54

Weight (kDa)

6.72

Isoelectric Point (pI)

59.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 238 - 383 3.5e-15 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 345
AccBSI CCGCTC 1 cut(s) 85
AccII CGCG 3 cut(s) 48, 458, 1020
AcoI YGGCCR 2 cut(s) 499, 773
AcuI CTGAAG 2 cut(s) 762, 1199
AcyI GRCGYC 1 cut(s) 346
AfaI GTAC 2 cut(s) 899, 985
AgsI TTSAA 1 cut(s) 853
AluBI AGCT 5 cut(s) 592, 817, 871, 1072, 1213
AluI AGCT 5 cut(s) 592, 817, 871, 1072, 1213
Alw21I GWGCWC 3 cut(s) 594, 782, 873
Alw26I GTCTC 1 cut(s) 188
AlwNI CAGNNNCTG 1 cut(s) 1075
AoxI GGCC 4 cut(s) 499, 528, 701, 773
ApeKI GCWGC 5 cut(s) 26, 640, 1069, 1072, 1174
AspLEI GCGC 7 cut(s) 48, 348, 482, 507, 889, 1012, 1022
AspS9I GGNCC 4 cut(s) 579, 597, 701, 857
AsuC2I CCSGG 4 cut(s) 330, 536, 841, 1286
AsuHPI GGTGA 3 cut(s) 106, 701, 1066
AsuII TTCGAA 1 cut(s) 1224
AvaII GGWCC 3 cut(s) 579, 597, 857
BanI GGYRCC 1 cut(s) 345
BanII GRGCYC 3 cut(s) 594, 873, 1063
Bbv12I GWGCWC 3 cut(s) 594, 782, 873
BbvCI CCTCAGC 1 cut(s) 104
BbvI GCAGC 5 cut(s) 38, 627, 1059, 1081, 1161
BccI CCATC 4 cut(s) 253, 401, 508, 872
BceAI ACGGC 3 cut(s) 325, 1056, 1099
BcgI CGANNNNNNTGC 2 cut(s) 408, 442
BcnI CCSGG 4 cut(s) 330, 536, 841, 1286
BcoDI GTCTC 1 cut(s) 188
BfaI CTAG 1 cut(s) 191
BfoI RGCGCY 2 cut(s) 349, 508
BglI GCCNNNNNGGC 1 cut(s) 536
Bme1390I CCNGG 4 cut(s) 330, 536, 841, 1286
Bme18I GGWCC 3 cut(s) 579, 597, 857
BmgT120I GGNCC 4 cut(s) 579, 597, 701, 857
BmiI GGNNCC 4 cut(s) 305, 347, 580, 702
BmrFI CCNGG 4 cut(s) 330, 536, 841, 1286
Bpu10I CCTNAGC 1 cut(s) 104
Bpu14I TTCGAA 1 cut(s) 1224
BpuEI CTTGAG 3 cut(s) 276, 1219, 1300
BpuMI CCSGG 4 cut(s) 330, 536, 841, 1286
BsaHI GRCGYC 1 cut(s) 346
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 4 cut(s) 328, 341, 840, 932
BsaWI WCCGGW 2 cut(s) 594, 790
BsaXI ACNNNNNCTCC 4 cut(s) 84, 114, 983, 1013
Bse118I RCCGGY 1 cut(s) 501
Bse1I ACTGG 1 cut(s) 1135
Bse3DI GCAATG 2 cut(s) 889, 1029
BseDI CCNNGG 4 cut(s) 328, 341, 840, 932
BseGI GGATG 6 cut(s) 393, 519, 763, 1186, 1243, 1267
BseMI GCAATG 2 cut(s) 889, 1029
BseMII CTCAG 5 cut(s) 118, 886, 908, 1020, 1067
BseNI ACTGG 1 cut(s) 1135
BseX3I CGGCCG 1 cut(s) 499
BseXI GCAGC 5 cut(s) 38, 627, 1059, 1081, 1161
BseYI CCCAGC 2 cut(s) 697, 904
Bsh1236I CGCG 3 cut(s) 48, 458, 1020
Bsh1285I CGRYCG 2 cut(s) 498, 502
BshFI GGCC 4 cut(s) 501, 530, 703, 775
BshNI GGYRCC 1 cut(s) 345
BsiEI CGRYCG 2 cut(s) 498, 502
BsiHKAI GWGCWC 3 cut(s) 594, 782, 873
BsiSI CCGG 9 cut(s) 330, 502, 535, 595, 791, 805, 840, 1178, 1285
BslFI GGGAC 3 cut(s) 592, 923, 1051
BsmAI GTCTC 1 cut(s) 188
BsmFI GGGAC 3 cut(s) 592, 923, 1051
BsmI GAATGC 1 cut(s) 302
BsnI GGCC 4 cut(s) 501, 530, 703, 775
Bso31I GGTCTC 1 cut(s) 188
Bsp119I TTCGAA 1 cut(s) 1224
Bsp1286I GDGCHC 4 cut(s) 594, 782, 873, 1063
Bsp143I GATC 2 cut(s) 88, 956
BspANI GGCC 4 cut(s) 501, 530, 703, 775
BspCNI CTCAG 5 cut(s) 117, 885, 907, 1019, 1068
BspFNI CGCG 3 cut(s) 48, 458, 1020
BspLI GGNNCC 4 cut(s) 305, 347, 580, 702
BspT104I TTCGAA 1 cut(s) 1224
BspT107I GGYRCC 1 cut(s) 345
BspTNI GGTCTC 1 cut(s) 188
BsrBI CCGCTC 1 cut(s) 85
BsrDI GCAATG 2 cut(s) 889, 1029
BsrFI RCCGGY 1 cut(s) 501
BsrI ACTGG 1 cut(s) 1135
BssAI RCCGGY 1 cut(s) 501
BssECI CCNNGG 4 cut(s) 328, 341, 840, 932
BssMI GATC 2 cut(s) 88, 956
BssNI GRCGYC 1 cut(s) 346
BssT1I CCWWGG 1 cut(s) 932
Bst4CI ACNGT 1 cut(s) 784
Bst6I CTCTTC 2 cut(s) 963, 1089
BstACI GRCGYC 1 cut(s) 346
BstBI TTCGAA 1 cut(s) 1224
BstC8I GCNNGC 6 cut(s) 53, 503, 532, 769, 773, 1269
BstDEI CTNAG 6 cut(s) 104, 872, 894, 1006, 1076, 1295
BstDSI CCRYGG 1 cut(s) 341
BstF5I GGATG 6 cut(s) 393, 519, 763, 1186, 1243, 1267
BstFNI CGCG 3 cut(s) 48, 458, 1020
BstH2I RGCGCY 2 cut(s) 349, 508
BstHHI GCGC 7 cut(s) 48, 348, 482, 507, 889, 1012, 1022
BstKTI GATC 2 cut(s) 91, 959
BstMAI GTCTC 1 cut(s) 188
BstMBI GATC 2 cut(s) 88, 956
BstMCI CGRYCG 2 cut(s) 498, 502
BstNSI RCATGY 2 cut(s) 364, 904
BstSCI CCNGG 4 cut(s) 328, 534, 839, 1284
BstUI CGCG 3 cut(s) 48, 458, 1020
BstV1I GCAGC 5 cut(s) 38, 627, 1059, 1081, 1161
BstZI CGGCCG 1 cut(s) 499
BsuRI GGCC 4 cut(s) 501, 530, 703, 775
BtgI CCRYGG 1 cut(s) 341
BtsCI GGATG 6 cut(s) 393, 519, 763, 1186, 1243, 1267
BtsI GCAGTG 1 cut(s) 475
BtsIMutI CAGTG 3 cut(s) 475, 789, 880
Cac8I GCNNGC 6 cut(s) 53, 503, 532, 769, 773, 1269
CaiI CAGNNNCTG 1 cut(s) 1075
CfoI GCGC 7 cut(s) 48, 348, 482, 507, 889, 1012, 1022
Cfr10I RCCGGY 1 cut(s) 501
Cfr13I GGNCC 4 cut(s) 579, 597, 701, 857
CpoI CGGWCCG 1 cut(s) 597
Csp6I GTAC 2 cut(s) 898, 984
CspI CGGWCCG 1 cut(s) 597
CviAII CATG 3 cut(s) 361, 901, 1013
CviQI GTAC 2 cut(s) 898, 984
DdeI CTNAG 6 cut(s) 104, 872, 894, 1006, 1076, 1295
DinI GGCGCC 1 cut(s) 347
DpnI GATC 2 cut(s) 90, 958
DpnII GATC 2 cut(s) 88, 956
EaeI YGGCCR 2 cut(s) 499, 773
EagI CGGCCG 1 cut(s) 499
Eam1104I CTCTTC 2 cut(s) 963, 1089
EarI CTCTTC 2 cut(s) 963, 1089
Ecl136II GAGCTC 2 cut(s) 592, 871
EclXI CGGCCG 1 cut(s) 499
Eco130I CCWWGG 1 cut(s) 932
Eco147I AGGCCT 1 cut(s) 530
Eco24I GRGCYC 3 cut(s) 594, 873, 1063
Eco31I GGTCTC 1 cut(s) 188
Eco47I GGWCC 3 cut(s) 579, 597, 857
Eco52I CGGCCG 1 cut(s) 499
Eco53kI GAGCTC 2 cut(s) 592, 871
Eco57I CTGAAG 2 cut(s) 762, 1199
EcoICRI GAGCTC 2 cut(s) 592, 871
EcoO109I RGGNCCY 1 cut(s) 579
EcoT14I CCWWGG 1 cut(s) 932
EcoT38I GRGCYC 3 cut(s) 594, 873, 1063
EgeI GGCGCC 1 cut(s) 347
EheI GGCGCC 1 cut(s) 347
ErhI CCWWGG 1 cut(s) 932
FaeI CATG 3 cut(s) 364, 904, 1016
FaiI YATR 6 cut(s) 362, 605, 867, 902, 951, 1014
FaqI GGGAC 3 cut(s) 592, 923, 1051
FatI CATG 3 cut(s) 360, 900, 1012
FokI GGATG 6 cut(s) 380, 526, 770, 1193, 1250, 1274
FriOI GRGCYC 3 cut(s) 594, 873, 1063
FspBI CTAG 1 cut(s) 191
GlaI GCGC 7 cut(s) 47, 347, 481, 506, 888, 1011, 1021
GsaI CCCAGC 2 cut(s) 701, 908
HaeII RGCGCY 2 cut(s) 349, 508
HaeIII GGCC 4 cut(s) 501, 530, 703, 775
HapII CCGG 9 cut(s) 330, 502, 535, 595, 791, 805, 840, 1178, 1285
HhaI GCGC 7 cut(s) 48, 348, 482, 507, 889, 1012, 1022
Hin1I GRCGYC 1 cut(s) 346
Hin1II CATG 3 cut(s) 364, 904, 1016
Hin6I GCGC 7 cut(s) 46, 346, 480, 505, 887, 1010, 1020
HinP1I GCGC 7 cut(s) 46, 346, 480, 505, 887, 1010, 1020
HinfI GANTC 7 cut(s) 335, 430, 518, 737, 836, 911, 943
HpaII CCGG 9 cut(s) 330, 502, 535, 595, 791, 805, 840, 1178, 1285
HphI GGTGA 3 cut(s) 106, 701, 1066
Hpy188I TCNGA 6 cut(s) 367, 411, 601, 612, 742, 1155
Hpy188III TCNNGA 3 cut(s) 293, 511, 755
HpyAV CCTTC 7 cut(s) 151, 395, 435, 737, 988, 1084, 1220
HpyCH4III ACNGT 1 cut(s) 784
HpyCH4V TGCA 1 cut(s) 555
HpyF3I CTNAG 6 cut(s) 104, 872, 894, 1006, 1076, 1295
Hsp92I GRCGYC 1 cut(s) 346
Hsp92II CATG 3 cut(s) 364, 904, 1016
HspAI GCGC 7 cut(s) 46, 346, 480, 505, 887, 1010, 1020
KasI GGCGCC 1 cut(s) 345
KroI GCCGGC 1 cut(s) 501
KroNI GCCGGC 1 cut(s) 503
Kzo9I GATC 2 cut(s) 88, 956
LmnI GCTCC 3 cut(s) 23, 597, 1210
Lsp1109I GCAGC 5 cut(s) 38, 627, 1059, 1081, 1161
MaeI CTAG 1 cut(s) 191
MaeIII GTNAC 2 cut(s) 112, 1126
MalI GATC 2 cut(s) 90, 958
MbiI CCGCTC 1 cut(s) 85
MboI GATC 2 cut(s) 88, 956
MboII GAAGA 3 cut(s) 70, 980, 1106
MhlI GDGCHC 4 cut(s) 594, 782, 873, 1063
MluCI AATT 3 cut(s) 572, 646, 1147
Mly113I GGCGCC 1 cut(s) 346
MlyI GAGTC 5 cut(s) 329, 512, 845, 905, 937
MroNI GCCGGC 1 cut(s) 501
MseI TTAA 1 cut(s) 1002
MspA1I CMGCKG 3 cut(s) 15, 1072, 1174
MspI CCGG 9 cut(s) 330, 502, 535, 595, 791, 805, 840, 1178, 1285
MspR9I CCNGG 4 cut(s) 330, 536, 841, 1286
Mva1269I GAATGC 1 cut(s) 302
MvnI CGCG 3 cut(s) 48, 458, 1020
NaeI GCCGGC 1 cut(s) 503
NarI GGCGCC 1 cut(s) 346
NciI CCSGG 4 cut(s) 330, 536, 841, 1286
NdeII GATC 2 cut(s) 88, 956
NgoMIV GCCGGC 1 cut(s) 501
NlaIII CATG 3 cut(s) 364, 904, 1016
NlaIV GGNNCC 4 cut(s) 305, 347, 580, 702
NmeAIII GCCGAG 2 cut(s) 500, 801
NmuCI GTSAC 1 cut(s) 112
NspI RCATGY 2 cut(s) 364, 904
NspV TTCGAA 1 cut(s) 1224
PceI AGGCCT 1 cut(s) 530
PcsI WCGNNNNNNNCGW 1 cut(s) 1046
PctI GAATGC 1 cut(s) 302
PdiI GCCGGC 1 cut(s) 503
PfeI GAWTC 2 cut(s) 430, 737
PleI GAGTC 5 cut(s) 329, 512, 844, 905, 937
PluTI GGCGCC 1 cut(s) 349
PpsI GAGTC 5 cut(s) 329, 512, 844, 905, 937
PpuMI RGGWCCY 1 cut(s) 579
Psp124BI GAGCTC 2 cut(s) 594, 873
Psp5II RGGWCCY 1 cut(s) 579
PspFI CCCAGC 2 cut(s) 697, 904
PspN4I GGNNCC 4 cut(s) 305, 347, 580, 702
PspPI GGNCC 4 cut(s) 579, 597, 701, 857
PspPPI RGGWCCY 1 cut(s) 579
PstNI CAGNNNCTG 1 cut(s) 1075
PvuII CAGCTG 1 cut(s) 1072
RsaI GTAC 2 cut(s) 899, 985
RsaNI GTAC 2 cut(s) 898, 984
Rsr2I CGGWCCG 1 cut(s) 597
RsrII CGGWCCG 1 cut(s) 597
SacI GAGCTC 2 cut(s) 594, 873
SaqAI TTAA 1 cut(s) 1002
Sau3AI GATC 2 cut(s) 88, 956
Sau96I GGNCC 4 cut(s) 579, 597, 701, 857
SchI GAGTC 5 cut(s) 329, 512, 845, 905, 937
ScrFI CCNGG 4 cut(s) 330, 536, 841, 1286
SduI GDGCHC 4 cut(s) 594, 782, 873, 1063
SfoI GGCGCC 1 cut(s) 347
SfuI TTCGAA 1 cut(s) 1224
SinI GGWCC 3 cut(s) 579, 597, 857
SmlI CTYRAG 3 cut(s) 291, 1198, 1279
SmoI CTYRAG 3 cut(s) 291, 1198, 1279
Sse9I AATT 3 cut(s) 572, 646, 1147
SseBI AGGCCT 1 cut(s) 530
SspDI GGCGCC 1 cut(s) 345
SspMI CTAG 1 cut(s) 191
SstI GAGCTC 2 cut(s) 594, 873
StuI AGGCCT 1 cut(s) 530
StyD4I CCNGG 4 cut(s) 328, 534, 839, 1284
StyI CCWWGG 1 cut(s) 932
TaaI ACNGT 1 cut(s) 784
TaqI TCGA 3 cut(s) 87, 428, 1224
TaqII GACCGA 1 cut(s) 550
TasI AATT 3 cut(s) 572, 646, 1147
TatI WGTACW 1 cut(s) 897
TfiI GAWTC 2 cut(s) 430, 737
Tru1I TTAA 1 cut(s) 1002
Tru9I TTAA 1 cut(s) 1002
TscAI CASTG 3 cut(s) 482, 789, 880
TseFI GTSAC 1 cut(s) 112
TseI GCWGC 5 cut(s) 26, 640, 1069, 1072, 1174
Tsp45I GTSAC 1 cut(s) 112
TspDTI ATGAA 1 cut(s) 1172
TspGWI ACGGA 2 cut(s) 538, 936
TspRI CASTG 3 cut(s) 482, 789, 880
VpaK11BI GGWCC 3 cut(s) 579, 597, 857
XceI RCATGY 2 cut(s) 364, 904
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.