Rh2CG025000

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0009592
Physical Location & Seq
Reverse (-)
1910913 .. 1912211
1299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG025000.1

Sequence Viewer

Length: 1299 bp
ATGTCCGCCGTCAGCGGCGGCGGAGCAGCCAAGAACACCGCCAAACGCGGCAAGCAGAAGAACAAGAACCACCACACTCCGCCGCTCGATCAACGAAGCCTCCTCAGCCTCGTCACCTCCGCCACCTCACTCGCCCACTCCTTCCTCTCCCAAAACGACCTCCACCTCCTCCCCTCCCAAACCCTAACCCTAGAGACCCTCCTCTCCTCCGCCTCCTCCGCCCTCTCCCCCCTTCTCCTCCTCTCATCTCCTCCGCCGCCGCCGCAACCACCGCCGCCTCCTCCTCAAGAATGCTGGTTCCGCCGCTTCCTCTCCTCCGCCGCCGGCGACTCGCCGTGGCGCCACGCTTTCCGCATGTGCGAACACTCGTTCTCTATCCTTCTCTCCATCCTCTCCCCATTTCTGACCTCCGCAATCCCTTCGATTCCGCCCAACTACGCCCTCGCCGCCGCGATTTACCGCCTCGCCCACGGCGCCACCTACAAGGCGGTCGGCCAGCGCTTCGGGATGGACTCGGCGGAGGCCTGCCGGGCTTTCTACGCCGTCTGCAAGTCGGTCAATGATAAATTAGGGAGCTTGTTTGCGCTCCGCTCCGATATGGCTCGGATTGTTGGGGGTTTCGGGTGGATTTCGCTGCCGAATTGCTGTGGGGTGTTAGGGTTTGGGAGATTTGGGGTTAGTGGTGAAATGCTGGGGCCAAATGGGTCGCTGTTGGTTCAGGCATTGGTGGATTCTGAAGGGAGGTTTCTGGATGTTTCGGCTGGCTGGCCGAGCACAGTGAAACCGGAAGCGATTTTCCGGCAGAGTAAGCTGTATTTGGGTGTGGAGGAGTCCAGAGAGTTGTTGAATGGTCCTGTTTATGAGCTCAGTGATGGCAATGCGCTTCCTCAGTACATACTGGGGGACTCTTGCTTTCCTCTGTTGCCTTGGCTTTTGACTCCGTATGTGAGATCAAATGAGGAAGAGGAGAGTTTCGGTACATTGGAGAAGGCGTTTAACTCGGCGCATGGTCGCGCAATGGGGCTGGTTGGGACGGCATTTGCGACGGTGAGGGCTCGGTGGCAGCTGCTGAGTAGGGAGTGGAAGGAAGAGTGTGTGGAGTTTTTGCCGTTTGTGGTGGTTACTGGCTGTCTGCTGAATAATTTTCTGATGAAGTGTAGTGAGCCGCTGCCGGATGACAATGTGGGGTGCTTGAGGGAGGAGGAGCTTCAGGTGTTCGAAGGACAGGTGGATGAGAGTGGCGAGAGGATTAGGGATGTGCTTGCCACGCACTTGAGCCGGGTAAGCCTAAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

432

Amino Acids

47.11

Weight (kDa)

6.52

Isoelectric Point (pI)

55.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 236 - 381 3.7e-15 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 339, 473
AccBSI CCGCTC 2 cut(s) 85, 591
AccII CGCG 3 cut(s) 48, 452, 1014
AcoI YGGCCR 2 cut(s) 493, 767
AcuI CTGAAG 2 cut(s) 756, 1193
AcyI GRCGYC 2 cut(s) 340, 474
AfaI GTAC 2 cut(s) 893, 979
AfeI AGCGCT 1 cut(s) 500
AgsI TTSAA 1 cut(s) 847
AluBI AGCT 5 cut(s) 576, 811, 865, 1066, 1207
AluI AGCT 5 cut(s) 576, 811, 865, 1066, 1207
Alw21I GWGCWC 2 cut(s) 776, 867
Alw26I GTCTC 1 cut(s) 188
AlwNI CAGNNNCTG 1 cut(s) 1069
Aor51HI AGCGCT 1 cut(s) 500
AoxI GGCC 4 cut(s) 493, 522, 695, 767
ApeKI GCWGC 5 cut(s) 26, 634, 1063, 1066, 1168
AspLEI GCGC 7 cut(s) 342, 476, 501, 586, 883, 1006, 1016
AspS9I GGNCC 2 cut(s) 695, 851
AsuC2I CCSGG 2 cut(s) 530, 1280
AsuHPI GGTGA 3 cut(s) 106, 695, 1060
AsuII TTCGAA 1 cut(s) 1218
AvaII GGWCC 1 cut(s) 851
BanI GGYRCC 2 cut(s) 339, 473
BanII GRGCYC 2 cut(s) 867, 1057
Bbv12I GWGCWC 2 cut(s) 776, 867
BbvCI CCTCAGC 1 cut(s) 104
BbvI GCAGC 5 cut(s) 38, 621, 1053, 1075, 1155
BccI CCATC 3 cut(s) 395, 502, 866
BceAI ACGGC 5 cut(s) 319, 487, 527, 1050, 1093
BcgI CGANNNNNNTGC 2 cut(s) 402, 436
BcnI CCSGG 2 cut(s) 530, 1280
BcoDI GTCTC 1 cut(s) 188
BfaI CTAG 1 cut(s) 191
BfoI RGCGCY 3 cut(s) 343, 477, 502
BglI GCCNNNNNGGC 1 cut(s) 530
Bme1390I CCNGG 2 cut(s) 530, 1280
Bme18I GGWCC 1 cut(s) 851
BmgT120I GGNCC 2 cut(s) 695, 851
BmiI GGNNCC 4 cut(s) 299, 341, 475, 696
BmrFI CCNGG 2 cut(s) 530, 1280
BmrI ACTGGG 1 cut(s) 908
BmuI ACTGGG 1 cut(s) 908
Bpu10I CCTNAGC 1 cut(s) 104
Bpu14I TTCGAA 1 cut(s) 1218
BpuEI CTTGAG 3 cut(s) 270, 1213, 1294
BpuMI CCSGG 2 cut(s) 530, 1280
BsaHI GRCGYC 2 cut(s) 340, 474
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 3 cut(s) 335, 469, 926
BsaWI WCCGGW 1 cut(s) 784
BsaXI ACNNNNNCTCC 4 cut(s) 84, 114, 977, 1007
Bse118I RCCGGY 1 cut(s) 323
Bse1I ACTGG 2 cut(s) 903, 1129
Bse3DI GCAATG 2 cut(s) 883, 1023
BseDI CCNNGG 3 cut(s) 335, 469, 926
BseGI GGATG 6 cut(s) 387, 513, 757, 1180, 1237, 1261
BseMI GCAATG 2 cut(s) 883, 1023
BseMII CTCAG 4 cut(s) 118, 880, 902, 1061
BseNI ACTGG 2 cut(s) 903, 1129
BseXI GCAGC 5 cut(s) 38, 621, 1053, 1075, 1155
BseYI CCCAGC 1 cut(s) 691
Bsh1236I CGCG 3 cut(s) 48, 452, 1014
Bsh1285I CGRYCG 1 cut(s) 492
BshFI GGCC 4 cut(s) 495, 524, 697, 769
BshNI GGYRCC 2 cut(s) 339, 473
BsiEI CGRYCG 1 cut(s) 492
BsiHKAI GWGCWC 2 cut(s) 776, 867
BsiSI CCGG 6 cut(s) 324, 529, 785, 799, 1172, 1279
BslFI GGGAC 2 cut(s) 917, 1045
BsmAI GTCTC 1 cut(s) 188
BsmFI GGGAC 2 cut(s) 917, 1045
BsmI GAATGC 1 cut(s) 296
BsnI GGCC 4 cut(s) 495, 524, 697, 769
Bso31I GGTCTC 1 cut(s) 188
Bsp119I TTCGAA 1 cut(s) 1218
Bsp1286I GDGCHC 3 cut(s) 776, 867, 1057
Bsp143I GATC 2 cut(s) 88, 950
BspANI GGCC 4 cut(s) 495, 524, 697, 769
BspCNI CTCAG 4 cut(s) 117, 879, 901, 1062
BspFNI CGCG 3 cut(s) 48, 452, 1014
BspLI GGNNCC 4 cut(s) 299, 341, 475, 696
BspT104I TTCGAA 1 cut(s) 1218
BspT107I GGYRCC 2 cut(s) 339, 473
BspTNI GGTCTC 1 cut(s) 188
BsrBI CCGCTC 2 cut(s) 85, 591
BsrDI GCAATG 2 cut(s) 883, 1023
BsrFI RCCGGY 1 cut(s) 323
BsrI ACTGG 2 cut(s) 903, 1129
BssAI RCCGGY 1 cut(s) 323
BssECI CCNNGG 3 cut(s) 335, 469, 926
BssMI GATC 2 cut(s) 88, 950
BssNI GRCGYC 2 cut(s) 340, 474
BssT1I CCWWGG 1 cut(s) 926
Bst4CI ACNGT 2 cut(s) 778, 1048
Bst6I CTCTTC 2 cut(s) 957, 1083
BstACI GRCGYC 2 cut(s) 340, 474
BstBI TTCGAA 1 cut(s) 1218
BstC8I GCNNGC 7 cut(s) 53, 325, 497, 526, 763, 767, 1263
BstDEI CTNAG 5 cut(s) 104, 866, 888, 1070, 1289
BstDSI CCRYGG 2 cut(s) 335, 469
BstF5I GGATG 6 cut(s) 387, 513, 757, 1180, 1237, 1261
BstFNI CGCG 3 cut(s) 48, 452, 1014
BstH2I RGCGCY 3 cut(s) 343, 477, 502
BstHHI GCGC 7 cut(s) 342, 476, 501, 586, 883, 1006, 1016
BstKTI GATC 2 cut(s) 91, 953
BstMAI GTCTC 1 cut(s) 188
BstMBI GATC 2 cut(s) 88, 950
BstMCI CGRYCG 1 cut(s) 492
BstNSI RCATGY 1 cut(s) 358
BstSCI CCNGG 2 cut(s) 528, 1278
BstUI CGCG 3 cut(s) 48, 452, 1014
BstV1I GCAGC 5 cut(s) 38, 621, 1053, 1075, 1155
BsuRI GGCC 4 cut(s) 495, 524, 697, 769
BtgI CCRYGG 2 cut(s) 335, 469
BtsCI GGATG 6 cut(s) 387, 513, 757, 1180, 1237, 1261
BtsIMutI CAGTG 2 cut(s) 783, 874
Cac8I GCNNGC 7 cut(s) 53, 325, 497, 526, 763, 767, 1263
CaiI CAGNNNCTG 1 cut(s) 1069
CfoI GCGC 7 cut(s) 342, 476, 501, 586, 883, 1006, 1016
Cfr10I RCCGGY 1 cut(s) 323
Cfr13I GGNCC 2 cut(s) 695, 851
Csp6I GTAC 2 cut(s) 892, 978
CviAII CATG 2 cut(s) 355, 1007
CviQI GTAC 2 cut(s) 892, 978
DdeI CTNAG 5 cut(s) 104, 866, 888, 1070, 1289
DinI GGCGCC 2 cut(s) 341, 475
DpnI GATC 2 cut(s) 90, 952
DpnII GATC 2 cut(s) 88, 950
EaeI YGGCCR 2 cut(s) 493, 767
Eam1104I CTCTTC 2 cut(s) 957, 1083
EarI CTCTTC 2 cut(s) 957, 1083
Ecl136II GAGCTC 1 cut(s) 865
Eco130I CCWWGG 1 cut(s) 926
Eco147I AGGCCT 1 cut(s) 524
Eco24I GRGCYC 2 cut(s) 867, 1057
Eco31I GGTCTC 1 cut(s) 188
Eco47I GGWCC 1 cut(s) 851
Eco47III AGCGCT 1 cut(s) 500
Eco53kI GAGCTC 1 cut(s) 865
Eco57I CTGAAG 2 cut(s) 756, 1193
EcoICRI GAGCTC 1 cut(s) 865
EcoT14I CCWWGG 1 cut(s) 926
EcoT38I GRGCYC 2 cut(s) 867, 1057
EgeI GGCGCC 2 cut(s) 341, 475
EheI GGCGCC 2 cut(s) 341, 475
ErhI CCWWGG 1 cut(s) 926
FaeI CATG 2 cut(s) 358, 1010
FaiI YATR 6 cut(s) 356, 599, 861, 896, 945, 1008
FaqI GGGAC 2 cut(s) 917, 1045
FatI CATG 2 cut(s) 354, 1006
FokI GGATG 6 cut(s) 374, 520, 764, 1187, 1244, 1268
FriOI GRGCYC 2 cut(s) 867, 1057
FspBI CTAG 1 cut(s) 191
GlaI GCGC 7 cut(s) 341, 475, 500, 585, 882, 1005, 1015
GsaI CCCAGC 1 cut(s) 695
HaeII RGCGCY 3 cut(s) 343, 477, 502
HaeIII GGCC 4 cut(s) 495, 524, 697, 769
HapII CCGG 6 cut(s) 324, 529, 785, 799, 1172, 1279
HhaI GCGC 7 cut(s) 342, 476, 501, 586, 883, 1006, 1016
Hin1I GRCGYC 2 cut(s) 340, 474
Hin1II CATG 2 cut(s) 358, 1010
Hin6I GCGC 7 cut(s) 340, 474, 499, 584, 881, 1004, 1014
HinP1I GCGC 7 cut(s) 340, 474, 499, 584, 881, 1004, 1014
HinfI GANTC 7 cut(s) 329, 424, 512, 731, 830, 905, 937
HpaII CCGG 6 cut(s) 324, 529, 785, 799, 1172, 1279
HphI GGTGA 3 cut(s) 106, 695, 1060
Hpy188I TCNGA 5 cut(s) 405, 595, 606, 736, 1149
Hpy188III TCNNGA 4 cut(s) 287, 505, 749, 834
Hpy99I CGWCG 1 cut(s) 1048
HpyAV CCTTC 8 cut(s) 151, 242, 389, 429, 731, 982, 1078, 1214
HpyCH4III ACNGT 2 cut(s) 778, 1048
HpyCH4V TGCA 1 cut(s) 549
HpyF3I CTNAG 5 cut(s) 104, 866, 888, 1070, 1289
Hsp92I GRCGYC 2 cut(s) 340, 474
Hsp92II CATG 2 cut(s) 358, 1010
HspAI GCGC 7 cut(s) 340, 474, 499, 584, 881, 1004, 1014
KasI GGCGCC 2 cut(s) 339, 473
KroI GCCGGC 1 cut(s) 323
KroNI GCCGGC 1 cut(s) 325
Kzo9I GATC 2 cut(s) 88, 950
LmnI GCTCC 5 cut(s) 23, 573, 591, 596, 1204
Lsp1109I GCAGC 5 cut(s) 38, 621, 1053, 1075, 1155
MaeI CTAG 1 cut(s) 191
MaeIII GTNAC 2 cut(s) 112, 1120
MalI GATC 2 cut(s) 90, 952
MbiI CCGCTC 2 cut(s) 85, 591
MboI GATC 2 cut(s) 88, 950
MboII GAAGA 3 cut(s) 70, 974, 1100
MhlI GDGCHC 3 cut(s) 776, 867, 1057
MluCI AATT 3 cut(s) 566, 640, 1141
Mly113I GGCGCC 2 cut(s) 340, 474
MlyI GAGTC 5 cut(s) 323, 506, 839, 899, 931
MreI CGCCGGCG 1 cut(s) 323
MroNI GCCGGC 1 cut(s) 323
MseI TTAA 1 cut(s) 996
MspA1I CMGCKG 3 cut(s) 15, 1066, 1168
MspI CCGG 6 cut(s) 324, 529, 785, 799, 1172, 1279
MspR9I CCNGG 2 cut(s) 530, 1280
Mva1269I GAATGC 1 cut(s) 296
MvnI CGCG 3 cut(s) 48, 452, 1014
NaeI GCCGGC 1 cut(s) 325
NarI GGCGCC 2 cut(s) 340, 474
NciI CCSGG 2 cut(s) 530, 1280
NdeII GATC 2 cut(s) 88, 950
NgoMIV GCCGGC 1 cut(s) 323
NlaIII CATG 2 cut(s) 358, 1010
NlaIV GGNNCC 4 cut(s) 299, 341, 475, 696
NmeAIII GCCGAG 3 cut(s) 494, 795, 980
NmuCI GTSAC 1 cut(s) 112
NspI RCATGY 1 cut(s) 358
NspV TTCGAA 1 cut(s) 1218
PceI AGGCCT 1 cut(s) 524
PcsI WCGNNNNNNNCGW 1 cut(s) 1040
PctI GAATGC 1 cut(s) 296
PdiI GCCGGC 1 cut(s) 325
PfeI GAWTC 2 cut(s) 424, 731
PleI GAGTC 5 cut(s) 323, 506, 838, 899, 931
PluTI GGCGCC 2 cut(s) 343, 477
PpsI GAGTC 5 cut(s) 323, 506, 838, 899, 931
Psp124BI GAGCTC 1 cut(s) 867
PspFI CCCAGC 1 cut(s) 691
PspN4I GGNNCC 4 cut(s) 299, 341, 475, 696
PspPI GGNCC 2 cut(s) 695, 851
PstNI CAGNNNCTG 1 cut(s) 1069
PvuII CAGCTG 1 cut(s) 1066
RsaI GTAC 2 cut(s) 893, 979
RsaNI GTAC 2 cut(s) 892, 978
SacI GAGCTC 1 cut(s) 867
SaqAI TTAA 1 cut(s) 996
Sau3AI GATC 2 cut(s) 88, 950
Sau96I GGNCC 2 cut(s) 695, 851
SchI GAGTC 5 cut(s) 323, 506, 839, 899, 931
ScrFI CCNGG 2 cut(s) 530, 1280
SduI GDGCHC 3 cut(s) 776, 867, 1057
SfoI GGCGCC 2 cut(s) 341, 475
SfuI TTCGAA 1 cut(s) 1218
SgrAI CRCCGGYG 1 cut(s) 323
SinI GGWCC 1 cut(s) 851
SmlI CTYRAG 3 cut(s) 285, 1192, 1273
SmoI CTYRAG 3 cut(s) 285, 1192, 1273
Sse9I AATT 3 cut(s) 566, 640, 1141
SseBI AGGCCT 1 cut(s) 524
SspDI GGCGCC 2 cut(s) 339, 473
SspMI CTAG 1 cut(s) 191
SstI GAGCTC 1 cut(s) 867
StuI AGGCCT 1 cut(s) 524
StyD4I CCNGG 2 cut(s) 528, 1278
StyI CCWWGG 1 cut(s) 926
TaaI ACNGT 2 cut(s) 778, 1048
TaqI TCGA 3 cut(s) 87, 422, 1218
TaqII GACCGA 1 cut(s) 544
TasI AATT 3 cut(s) 566, 640, 1141
TatI WGTACW 1 cut(s) 891
TfiI GAWTC 2 cut(s) 424, 731
Tru1I TTAA 1 cut(s) 996
Tru9I TTAA 1 cut(s) 996
TscAI CASTG 2 cut(s) 783, 874
TseFI GTSAC 1 cut(s) 112
TseI GCWGC 5 cut(s) 26, 634, 1063, 1066, 1168
Tsp45I GTSAC 1 cut(s) 112
TspDTI ATGAA 1 cut(s) 1166
TspGWI ACGGA 1 cut(s) 930
TspRI CASTG 2 cut(s) 783, 874
VpaK11BI GGWCC 1 cut(s) 851
XceI RCATGY 1 cut(s) 358
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.