Rh2DG024900

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0009592
Physical Location & Seq
Reverse (-)
1472785 .. 1474179
1395 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG024900.1

Sequence Viewer

Length: 1395 bp
ATGCCTCTCTCATTTGACTCATTCACACACTCTTTAAAGTTTAAACACTTCACACTGAACTCTCCGGCCACTCTAAACTCTCTCAGTAACCAAACTCCACCGCCGATGTCCGCCGTCAGCGGCGGCGGAGCAGCCAAGAACACCGCCAAACGCGGCAAGCGGAAGAACAAGAACCACCACACTCCGCCGCTCGATCAACGAAACCTCCTCAGCCTCGTCACCTCCGCCACCTCACTCGCCCACTCCTTCCTCTCCCAAAACGACCTCCACCTCCTCCCCTCCCAAACCCTAACCCTAGAGACCCTCCTCTCCTCCGCCTCCTCCGCCCTCTCCCCCCTCCTCCTCCTCCCATCTCCTCCTCCGCCGCCGCCGCCTCCTCCTCAAGAATGCTGGTTCCGCCGCTTCCTCTCCTCCGCCCCCGGCGACTCGCCGTGGCGCCACGCTTTCCGCATGTCCGAACACTCGTTCTCTATCCTTCTCTCCATCCTCTCCCCGTTTCTGACCTCCGCAATCCCTTCGATTCCGCCCAACTACGCCCTCGCCGCCGCGATTTACCGCCTCGCCCACGGCGCCACCTACAAGGCGGTCGGCCGGCGCTTCGGGATGGACTCGGCGGAGGCCTGCCGGGCTTTCTACGCCGTCTGCAAGTCGGTCAATGATAAATTAGGGAGCTTGTTTGAGCTCCGGTCCGATATGGCTCGGATTGTTGGGGGTTTCGGGTGGATTTCGCTGCCGAATTGCTGTGGGGTGTTAGGGTTTGGGAGATTTGGGGTTAGTGGTGAAATGCTGGGGCCAAATGGGTCGCTGTTGGTTCAGGCATTGGTGGATTCTGAAGGGAGGTTTCTGGATGTTTCGGCTGGCTGGCCGAGCACAGTGAAACCGGAAGCGATTTTCCGGCAGAGTAAGCTGTATTTGGGTGTGGAGGAGTCCGGGGAGTTGTTGAATGGTCCTGTTTATGAGCTCAGTGATGGCAATGCGCTTCCTCAGTACATACTGGGGGACTCTTGCTTTCCTCTGCTGCCTTGGCTTTTGACTCCGTATGTGAGATCAAATGAGGAAGAGGAGAGTTTCGGTACACTGGAGAAGGCGTTTAACTCGGCGCATGGTCGCGCAATGGGGCTGGTTGGGACGGCGTTTGCGAGGGTGAGGGCTCGGTGGCAGCTGCTGAGTAGGGAGTGGAAGGAAGAGTGTGTGGAGTTTTTGCCGTTTGTGGTGGTTACTGGCTGTCTGCTGAATAATTTTCTGATGAAGTGTAGTGAGCCGCCGCCGGATGACAATGTGGGGTGCTTGAGGGAGGAGGAGCTTCAGGTGTTCGAAGGACAGGTGGATGAGAGTGGCGAGAGGATTAGGGATGTGCTTGCCGCGCACTTGAGCCGGGTAAGCCTAAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

464

Amino Acids

50.75

Weight (kDa)

7.15

Isoelectric Point (pI)

56.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 268 - 413 1.2e-15 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 435, 569
AccBSI CCGCTC 1 cut(s) 190
AccII CGCG 4 cut(s) 153, 548, 1110, 1364
AcoI YGGCCR 3 cut(s) 66, 589, 863
AcuI CTGAAG 2 cut(s) 852, 1289
AcyI GRCGYC 2 cut(s) 436, 570
AfaI GTAC 2 cut(s) 989, 1075
AgsI TTSAA 1 cut(s) 943
AluBI AGCT 6 cut(s) 672, 682, 907, 961, 1162, 1303
AluI AGCT 6 cut(s) 672, 682, 907, 961, 1162, 1303
Alw21I GWGCWC 3 cut(s) 684, 872, 963
Alw26I GTCTC 1 cut(s) 293
AlwNI CAGNNNCTG 1 cut(s) 1165
AoxI GGCC 5 cut(s) 66, 589, 618, 791, 863
ApeKI GCWGC 5 cut(s) 131, 730, 1018, 1159, 1162
AspLEI GCGC 7 cut(s) 438, 572, 597, 979, 1102, 1112, 1366
AspS9I GGNCC 3 cut(s) 687, 791, 947
AsuC2I CCSGG 4 cut(s) 420, 626, 931, 1376
AsuHPI GGTGA 3 cut(s) 211, 791, 1156
AsuII TTCGAA 1 cut(s) 1314
AvaII GGWCC 2 cut(s) 687, 947
BanI GGYRCC 2 cut(s) 435, 569
BanII GRGCYC 3 cut(s) 684, 963, 1153
Bbv12I GWGCWC 3 cut(s) 684, 872, 963
BbvCI CCTCAGC 1 cut(s) 209
BbvI GCAGC 5 cut(s) 143, 717, 1005, 1149, 1171
BccI CCATC 4 cut(s) 358, 491, 598, 962
BceAI ACGGC 6 cut(s) 98, 415, 583, 623, 1146, 1189
BcgI CGANNNNNNTGC 2 cut(s) 498, 532
BcnI CCSGG 4 cut(s) 420, 626, 931, 1376
BcoDI GTCTC 1 cut(s) 293
BfaI CTAG 1 cut(s) 296
BfoI RGCGCY 3 cut(s) 439, 573, 598
BglI GCCNNNNNGGC 1 cut(s) 626
Bme1390I CCNGG 4 cut(s) 420, 626, 931, 1376
Bme18I GGWCC 2 cut(s) 687, 947
BmgT120I GGNCC 3 cut(s) 687, 791, 947
BmiI GGNNCC 4 cut(s) 395, 437, 571, 792
BmrFI CCNGG 4 cut(s) 420, 626, 931, 1376
BmrI ACTGGG 1 cut(s) 1004
BmuI ACTGGG 1 cut(s) 1004
BpmI CTGGAG 1 cut(s) 1100
Bpu10I CCTNAGC 1 cut(s) 209
Bpu14I TTCGAA 1 cut(s) 1314
BpuEI CTTGAG 3 cut(s) 366, 1309, 1390
BpuMI CCSGG 4 cut(s) 420, 626, 931, 1376
BsaHI GRCGYC 2 cut(s) 436, 570
BsaI GGTCTC 1 cut(s) 293
BsaJI CCNNGG 5 cut(s) 418, 431, 565, 930, 1022
BsaWI WCCGGW 2 cut(s) 684, 880
BsaXI ACNNNNNCTCC 4 cut(s) 189, 219, 1073, 1103
Bse118I RCCGGY 1 cut(s) 591
Bse1I ACTGG 3 cut(s) 999, 1083, 1225
Bse3DI GCAATG 2 cut(s) 979, 1119
BseDI CCNNGG 5 cut(s) 418, 431, 565, 930, 1022
BseGI GGATG 6 cut(s) 483, 609, 853, 1276, 1333, 1357
BseMI GCAATG 2 cut(s) 979, 1119
BseMII CTCAG 5 cut(s) 97, 223, 976, 998, 1157
BseNI ACTGG 3 cut(s) 999, 1083, 1225
BseX3I CGGCCG 1 cut(s) 589
BseXI GCAGC 5 cut(s) 143, 717, 1005, 1149, 1171
BseYI CCCAGC 1 cut(s) 787
Bsh1236I CGCG 4 cut(s) 153, 548, 1110, 1364
Bsh1285I CGRYCG 2 cut(s) 588, 592
BshFI GGCC 5 cut(s) 68, 591, 620, 793, 865
BshNI GGYRCC 2 cut(s) 435, 569
BsiEI CGRYCG 2 cut(s) 588, 592
BsiHKAI GWGCWC 3 cut(s) 684, 872, 963
BslFI GGGAC 2 cut(s) 1013, 1141
BsmAI GTCTC 1 cut(s) 293
BsmFI GGGAC 2 cut(s) 1013, 1141
BsmI GAATGC 1 cut(s) 392
BsnI GGCC 5 cut(s) 68, 591, 620, 793, 865
Bso31I GGTCTC 1 cut(s) 293
Bsp119I TTCGAA 1 cut(s) 1314
Bsp1286I GDGCHC 4 cut(s) 684, 872, 963, 1153
Bsp143I GATC 2 cut(s) 193, 1046
BspANI GGCC 5 cut(s) 68, 591, 620, 793, 865
BspCNI CTCAG 5 cut(s) 96, 222, 975, 997, 1158
BspFNI CGCG 4 cut(s) 153, 548, 1110, 1364
BspLI GGNNCC 4 cut(s) 395, 437, 571, 792
BspT104I TTCGAA 1 cut(s) 1314
BspT107I GGYRCC 2 cut(s) 435, 569
BspTNI GGTCTC 1 cut(s) 293
BsrBI CCGCTC 1 cut(s) 190
BsrDI GCAATG 2 cut(s) 979, 1119
BsrFI RCCGGY 1 cut(s) 591
BsrI ACTGG 3 cut(s) 999, 1083, 1225
BssAI RCCGGY 1 cut(s) 591
BssECI CCNNGG 5 cut(s) 418, 431, 565, 930, 1022
BssMI GATC 2 cut(s) 193, 1046
BssNI GRCGYC 2 cut(s) 436, 570
BssT1I CCWWGG 1 cut(s) 1022
Bst4CI ACNGT 1 cut(s) 874
Bst6I CTCTTC 2 cut(s) 1053, 1179
BstACI GRCGYC 2 cut(s) 436, 570
BstBI TTCGAA 1 cut(s) 1314
BstC8I GCNNGC 6 cut(s) 158, 593, 622, 859, 863, 1359
BstDEI CTNAG 6 cut(s) 83, 209, 962, 984, 1166, 1385
BstDSI CCRYGG 2 cut(s) 431, 565
BstF5I GGATG 6 cut(s) 483, 609, 853, 1276, 1333, 1357
BstFNI CGCG 4 cut(s) 153, 548, 1110, 1364
BstH2I RGCGCY 3 cut(s) 439, 573, 598
BstHHI GCGC 7 cut(s) 438, 572, 597, 979, 1102, 1112, 1366
BstKTI GATC 2 cut(s) 196, 1049
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 2 cut(s) 193, 1046
BstMCI CGRYCG 2 cut(s) 588, 592
BstNSI RCATGY 1 cut(s) 454
BstSCI CCNGG 4 cut(s) 418, 624, 929, 1374
BstUI CGCG 4 cut(s) 153, 548, 1110, 1364
BstV1I GCAGC 5 cut(s) 143, 717, 1005, 1149, 1171
BstZI CGGCCG 1 cut(s) 589
BsuRI GGCC 5 cut(s) 68, 591, 620, 793, 865
BtgI CCRYGG 2 cut(s) 431, 565
BtsCI GGATG 6 cut(s) 483, 609, 853, 1276, 1333, 1357
BtsIMutI CAGTG 4 cut(s) 53, 879, 970, 1076
Cac8I GCNNGC 6 cut(s) 158, 593, 622, 859, 863, 1359
CaiI CAGNNNCTG 1 cut(s) 1165
CfoI GCGC 7 cut(s) 438, 572, 597, 979, 1102, 1112, 1366
Cfr10I RCCGGY 1 cut(s) 591
Cfr13I GGNCC 3 cut(s) 687, 791, 947
CpoI CGGWCCG 1 cut(s) 687
Csp6I GTAC 2 cut(s) 988, 1074
CspI CGGWCCG 1 cut(s) 687
CviAII CATG 2 cut(s) 451, 1103
CviQI GTAC 2 cut(s) 988, 1074
DdeI CTNAG 6 cut(s) 83, 209, 962, 984, 1166, 1385
DinI GGCGCC 2 cut(s) 437, 571
DpnI GATC 2 cut(s) 195, 1048
DpnII GATC 2 cut(s) 193, 1046
DraI TTTAAA 2 cut(s) 36, 43
EaeI YGGCCR 3 cut(s) 66, 589, 863
EagI CGGCCG 1 cut(s) 589
Eam1104I CTCTTC 2 cut(s) 1053, 1179
EarI CTCTTC 2 cut(s) 1053, 1179
Ecl136II GAGCTC 2 cut(s) 682, 961
EclXI CGGCCG 1 cut(s) 589
Eco130I CCWWGG 1 cut(s) 1022
Eco147I AGGCCT 1 cut(s) 620
Eco24I GRGCYC 3 cut(s) 684, 963, 1153
Eco31I GGTCTC 1 cut(s) 293
Eco47I GGWCC 2 cut(s) 687, 947
Eco52I CGGCCG 1 cut(s) 589
Eco53kI GAGCTC 2 cut(s) 682, 961
Eco57I CTGAAG 2 cut(s) 852, 1289
EcoICRI GAGCTC 2 cut(s) 682, 961
EcoT14I CCWWGG 1 cut(s) 1022
EcoT38I GRGCYC 3 cut(s) 684, 963, 1153
EgeI GGCGCC 2 cut(s) 437, 571
EheI GGCGCC 2 cut(s) 437, 571
ErhI CCWWGG 1 cut(s) 1022
FaeI CATG 2 cut(s) 454, 1106
FaiI YATR 6 cut(s) 452, 695, 957, 992, 1041, 1104
FaqI GGGAC 2 cut(s) 1013, 1141
FatI CATG 2 cut(s) 450, 1102
FokI GGATG 6 cut(s) 470, 616, 860, 1283, 1340, 1364
FriOI GRGCYC 3 cut(s) 684, 963, 1153
FspBI CTAG 1 cut(s) 296
GlaI GCGC 7 cut(s) 437, 571, 596, 978, 1101, 1111, 1365
GsaI CCCAGC 1 cut(s) 791
GsuI CTGGAG 1 cut(s) 1100
HaeII RGCGCY 3 cut(s) 439, 573, 598
HaeIII GGCC 5 cut(s) 68, 591, 620, 793, 865
HhaI GCGC 7 cut(s) 438, 572, 597, 979, 1102, 1112, 1366
Hin1I GRCGYC 2 cut(s) 436, 570
Hin1II CATG 2 cut(s) 454, 1106
Hin6I GCGC 7 cut(s) 436, 570, 595, 977, 1100, 1110, 1364
HinP1I GCGC 7 cut(s) 436, 570, 595, 977, 1100, 1110, 1364
HinfI GANTC 8 cut(s) 17, 425, 520, 608, 827, 926, 1001, 1033
HphI GGTGA 3 cut(s) 211, 791, 1156
Hpy166II GTNNAC 1 cut(s) 1076
Hpy188I TCNGA 6 cut(s) 457, 501, 691, 702, 832, 1245
Hpy188III TCNNGA 3 cut(s) 383, 601, 845
Hpy8I GTNNAC 1 cut(s) 1076
HpyAV CCTTC 7 cut(s) 256, 485, 525, 827, 1078, 1174, 1310
HpyCH4III ACNGT 1 cut(s) 874
HpyCH4V TGCA 1 cut(s) 645
HpyF3I CTNAG 6 cut(s) 83, 209, 962, 984, 1166, 1385
Hsp92I GRCGYC 2 cut(s) 436, 570
Hsp92II CATG 2 cut(s) 454, 1106
HspAI GCGC 7 cut(s) 436, 570, 595, 977, 1100, 1110, 1364
KasI GGCGCC 2 cut(s) 435, 569
KroI GCCGGC 1 cut(s) 591
KroNI GCCGGC 1 cut(s) 593
Kzo9I GATC 2 cut(s) 193, 1046
LmnI GCTCC 4 cut(s) 128, 669, 687, 1300
Lsp1109I GCAGC 5 cut(s) 143, 717, 1005, 1149, 1171
MaeI CTAG 1 cut(s) 296
MaeIII GTNAC 3 cut(s) 86, 217, 1216
MalI GATC 2 cut(s) 195, 1048
MbiI CCGCTC 1 cut(s) 190
MboI GATC 2 cut(s) 193, 1046
MboII GAAGA 3 cut(s) 175, 1070, 1196
MhlI GDGCHC 4 cut(s) 684, 872, 963, 1153
MluCI AATT 3 cut(s) 662, 736, 1237
Mly113I GGCGCC 2 cut(s) 436, 570
MlyI GAGTC 6 cut(s) 11, 419, 602, 935, 995, 1027
MroNI GCCGGC 1 cut(s) 591
MseI TTAA 3 cut(s) 35, 42, 1092
MspA1I CMGCKG 2 cut(s) 120, 1162
MspR9I CCNGG 4 cut(s) 420, 626, 931, 1376
MssI GTTTAAAC 1 cut(s) 43
Mva1269I GAATGC 1 cut(s) 392
MvnI CGCG 4 cut(s) 153, 548, 1110, 1364
NaeI GCCGGC 1 cut(s) 593
NarI GGCGCC 2 cut(s) 436, 570
NciI CCSGG 4 cut(s) 420, 626, 931, 1376
NdeII GATC 2 cut(s) 193, 1046
NgoMIV GCCGGC 1 cut(s) 591
NlaIII CATG 2 cut(s) 454, 1106
NlaIV GGNNCC 4 cut(s) 395, 437, 571, 792
NmeAIII GCCGAG 3 cut(s) 590, 891, 1076
NmuCI GTSAC 1 cut(s) 217
NspI RCATGY 1 cut(s) 454
NspV TTCGAA 1 cut(s) 1314
PceI AGGCCT 1 cut(s) 620
PcsI WCGNNNNNNNCGW 1 cut(s) 1136
PctI GAATGC 1 cut(s) 392
PdiI GCCGGC 1 cut(s) 593
PfeI GAWTC 2 cut(s) 520, 827
PleI GAGTC 6 cut(s) 11, 419, 602, 934, 995, 1027
PluTI GGCGCC 2 cut(s) 439, 573
PmeI GTTTAAAC 1 cut(s) 43
PpsI GAGTC 6 cut(s) 11, 419, 602, 934, 995, 1027
Psp124BI GAGCTC 2 cut(s) 684, 963
PspFI CCCAGC 1 cut(s) 787
PspN4I GGNNCC 4 cut(s) 395, 437, 571, 792
PspPI GGNCC 3 cut(s) 687, 791, 947
PstNI CAGNNNCTG 1 cut(s) 1165
PvuII CAGCTG 1 cut(s) 1162
RsaI GTAC 2 cut(s) 989, 1075
RsaNI GTAC 2 cut(s) 988, 1074
Rsr2I CGGWCCG 1 cut(s) 687
RsrII CGGWCCG 1 cut(s) 687
SacI GAGCTC 2 cut(s) 684, 963
SaqAI TTAA 3 cut(s) 35, 42, 1092
Sau3AI GATC 2 cut(s) 193, 1046
Sau96I GGNCC 3 cut(s) 687, 791, 947
SchI GAGTC 6 cut(s) 11, 419, 602, 935, 995, 1027
ScrFI CCNGG 4 cut(s) 420, 626, 931, 1376
SduI GDGCHC 4 cut(s) 684, 872, 963, 1153
SfoI GGCGCC 2 cut(s) 437, 571
SfuI TTCGAA 1 cut(s) 1314
SinI GGWCC 2 cut(s) 687, 947
SmlI CTYRAG 3 cut(s) 381, 1288, 1369
SmoI CTYRAG 3 cut(s) 381, 1288, 1369
Sse9I AATT 3 cut(s) 662, 736, 1237
SseBI AGGCCT 1 cut(s) 620
SspDI GGCGCC 2 cut(s) 435, 569
SspMI CTAG 1 cut(s) 296
SstI GAGCTC 2 cut(s) 684, 963
StuI AGGCCT 1 cut(s) 620
StyD4I CCNGG 4 cut(s) 418, 624, 929, 1374
StyI CCWWGG 1 cut(s) 1022
TaaI ACNGT 1 cut(s) 874
TaqI TCGA 3 cut(s) 192, 518, 1314
TaqII GACCGA 1 cut(s) 640
TasI AATT 3 cut(s) 662, 736, 1237
TatI WGTACW 1 cut(s) 987
TfiI GAWTC 2 cut(s) 520, 827
Tru1I TTAA 3 cut(s) 35, 42, 1092
Tru9I TTAA 3 cut(s) 35, 42, 1092
TscAI CASTG 4 cut(s) 60, 879, 970, 1083
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 5 cut(s) 131, 730, 1018, 1159, 1162
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 1 cut(s) 1262
TspGWI ACGGA 1 cut(s) 1026
TspRI CASTG 4 cut(s) 60, 879, 970, 1083
VpaK11BI GGWCC 2 cut(s) 687, 947
XceI RCATGY 1 cut(s) 454
XspI CTAG 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.