RLG00000015823

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0009592
Physical Location & Seq
Reverse (-)
1662142 .. 1664182
2041 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015823

Sequence Viewer

Length: 1296 bp
ATGGCCGCCGTCAGCGGCGGCGGAGCAGCCAAGAACACCGCCAAACGCAGCAAGCAGAAGAACAAGAACCACCACACTCCGCCGCTCGATCAACGAAACCTGCTCAGCCTCGTCACCTCCGCCACCTCACTCGCCCACTCCTTCCTCACCCAAAACGACCTCCTCCTCCTCCCCTCCCAAACCCTAACCCTAGAGACCCTCCTCTCCTCCGCCTCCTCCGCCCTCTCCCCCCTCCTCCTCCTCTCATCTCCGCCACCGCCACAACCGCTGCCTCCTCCTCCTCAAGAATGCTGGTTCCGCCGCTTCCTCTCCTCCCCCGCCGGCGACTCGCCGTGGCGCCACGCTTTCCGCATGTCCGAACACTCGTTCTCTATCCTTCTGTCCATCCTCTCCCCATTTCTGACCTCCGCAATCCCTTCGATTCCACCCAACTACGCCCTCGCCGCCGCGATTTACCGCCTCGCCCACGGAGCCACCTACAAGGCGGTGGGCCGCCGCTTCGGGATGGACTCGGCGGAGGCCTGCCGGGCTTTCTACGCCGTCTGCAAGTCTGTCAATGATAAATTAGGGACCTTGTTTGAGCTCCGGTCCGATATGGCTCGGATTGTTGGGGGTTTCGGGTGGATTTCGCTGCCGAATTGCTGTGGGGTGTTAGGGTTTGGGAGATTTGGGGTTAGTGGTGAAATGCTGGGGCCGAATGGGTCGCTGTTGGTTCAGGCATTGGTGGATTCTGAAGGGAGGTTTCTGGATGTTTCGGCTGGCTGGCCGAGCACAGTGAAACCGGAAGCGATTTTCCGGCAGAGTAAGCTGTATTTGGGTGTGGAGGAGTCCAGGGAGTTGTTGAATGGTCCTGTTTATGAGCTTAGTGATGGCAATGCGCTTCCTCAGTACATATTGGGGGACTGTTGCTTTCCTCTGTTGCCTTGGCTTTTGACTCCGTATGTAAGATCAAATGAGGAAGAGGAGAGTTTCGATACATTGGAGAAGGGGTTTAACTCGGCGCATAGTCGCGCAATGGGGCTGGTTGGGACGGCATTTGCGAGGGTGAGGGCTCGGTGGCAGCTGCTGAGTAGGGAGTGGAAGGAAGAGTGTGTGGAGTTTTTGCCGTTTGTGGTGGTTACTGGCTGTCTGCTGAATAATTTTCTGATGAAGTGTAGTGAGGCGCTGCCGGATGACAATGTGGGGTGCTTGAGGGAGGAGGAGCTTCAGGTGTTCGAAGGACAGGTGGATGAGAGTGGCGAGAGGATTAGGGATGTGCTTGCCACACACTTGAGCCGGGTAAGCCTAAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

432

Amino Acids

47.29

Weight (kDa)

6.47

Isoelectric Point (pI)

57.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 235 - 380 6.6e-16 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 108
AccB1I GGYRCC 1 cut(s) 336
AccBSI CCGCTC 1 cut(s) 85
AccII CGCG 2 cut(s) 449, 1011
AcoI YGGCCR 2 cut(s) 3, 764
AcuI CTGAAG 2 cut(s) 753, 1190
AcyI GRCGYC 1 cut(s) 337
AfaI GTAC 1 cut(s) 890
AgsI TTSAA 1 cut(s) 844
AjnI CCWGG 1 cut(s) 830
AluBI AGCT 5 cut(s) 583, 808, 862, 1063, 1204
AluI AGCT 5 cut(s) 583, 808, 862, 1063, 1204
Alw21I GWGCWC 2 cut(s) 585, 773
Alw26I GTCTC 1 cut(s) 188
AlwNI CAGNNNCTG 1 cut(s) 1066
AoxI GGCC 5 cut(s) 3, 490, 519, 692, 764
ApeKI GCWGC 7 cut(s) 26, 48, 268, 631, 1060, 1063, 1165
AspLEI GCGC 5 cut(s) 339, 880, 1003, 1013, 1165
AspS9I GGNCC 5 cut(s) 490, 570, 588, 692, 848
AsuC2I CCSGG 2 cut(s) 527, 1277
AsuHPI GGTGA 4 cut(s) 106, 139, 692, 1057
AsuII TTCGAA 1 cut(s) 1215
AvaII GGWCC 3 cut(s) 570, 588, 848
BanI GGYRCC 1 cut(s) 336
BanII GRGCYC 2 cut(s) 585, 1054
Bbv12I GWGCWC 2 cut(s) 585, 773
BbvI GCAGC 7 cut(s) 38, 60, 255, 618, 1050, 1072, 1152
BccI CCATC 3 cut(s) 392, 499, 863
BceAI ACGGC 4 cut(s) 316, 524, 1047, 1090
BcgI CGANNNNNNTGC 2 cut(s) 399, 433
BciT130I CCWGG 1 cut(s) 832
BcnI CCSGG 2 cut(s) 527, 1277
BcoDI GTCTC 1 cut(s) 188
BfaI CTAG 1 cut(s) 191
BfoI RGCGCY 2 cut(s) 340, 1166
BfuAI ACCTGC 1 cut(s) 108
BglI GCCNNNNNGGC 1 cut(s) 527
BlpI GCTNAGC 1 cut(s) 104
Bme1390I CCNGG 3 cut(s) 527, 832, 1277
Bme18I GGWCC 3 cut(s) 570, 588, 848
BmgT120I GGNCC 5 cut(s) 490, 570, 588, 692, 848
BmiI GGNNCC 5 cut(s) 296, 338, 472, 571, 693
BmrFI CCNGG 3 cut(s) 527, 832, 1277
Bpu1102I GCTNAGC 1 cut(s) 104
Bpu14I TTCGAA 1 cut(s) 1215
BpuEI CTTGAG 3 cut(s) 267, 1210, 1291
BpuMI CCSGG 2 cut(s) 527, 1277
BsaHI GRCGYC 1 cut(s) 337
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 4 cut(s) 332, 466, 831, 923
BsaWI WCCGGW 2 cut(s) 585, 781
BsaXI ACNNNNNCTCC 2 cut(s) 974, 1004
Bse118I RCCGGY 1 cut(s) 320
Bse1I ACTGG 1 cut(s) 1126
Bse3DI GCAATG 2 cut(s) 880, 1020
BseBI CCWGG 1 cut(s) 832
BseDI CCNNGG 4 cut(s) 332, 466, 831, 923
BseGI GGATG 6 cut(s) 384, 510, 754, 1177, 1234, 1258
BseMI GCAATG 2 cut(s) 880, 1020
BseMII CTCAG 3 cut(s) 118, 899, 1058
BseNI ACTGG 1 cut(s) 1126
BseXI GCAGC 7 cut(s) 38, 60, 255, 618, 1050, 1072, 1152
BseYI CCCAGC 1 cut(s) 688
Bsh1236I CGCG 2 cut(s) 449, 1011
BshFI GGCC 5 cut(s) 5, 492, 521, 694, 766
BshNI GGYRCC 1 cut(s) 336
BsiHKAI GWGCWC 2 cut(s) 585, 773
BsiSI CCGG 7 cut(s) 321, 526, 586, 782, 796, 1169, 1276
BslFI GGGAC 3 cut(s) 583, 914, 1042
BsmAI GTCTC 1 cut(s) 188
BsmFI GGGAC 3 cut(s) 583, 914, 1042
BsmI GAATGC 1 cut(s) 293
BsnI GGCC 5 cut(s) 5, 492, 521, 694, 766
Bso31I GGTCTC 1 cut(s) 188
Bsp119I TTCGAA 1 cut(s) 1215
Bsp1286I GDGCHC 3 cut(s) 585, 773, 1054
Bsp143I GATC 2 cut(s) 88, 947
Bsp1720I GCTNAGC 1 cut(s) 104
BspANI GGCC 5 cut(s) 5, 492, 521, 694, 766
BspCNI CTCAG 3 cut(s) 117, 898, 1059
BspFNI CGCG 2 cut(s) 449, 1011
BspLI GGNNCC 5 cut(s) 296, 338, 472, 571, 693
BspMI ACCTGC 1 cut(s) 108
BspT104I TTCGAA 1 cut(s) 1215
BspT107I GGYRCC 1 cut(s) 336
BspTNI GGTCTC 1 cut(s) 188
BsrBI CCGCTC 1 cut(s) 85
BsrDI GCAATG 2 cut(s) 880, 1020
BsrFI RCCGGY 1 cut(s) 320
BsrI ACTGG 1 cut(s) 1126
BssAI RCCGGY 1 cut(s) 320
BssECI CCNNGG 4 cut(s) 332, 466, 831, 923
BssMI GATC 2 cut(s) 88, 947
BssNI GRCGYC 1 cut(s) 337
BssT1I CCWWGG 1 cut(s) 923
Bst2UI CCWGG 1 cut(s) 832
Bst4CI ACNGT 2 cut(s) 775, 905
Bst6I CTCTTC 2 cut(s) 954, 1080
BstACI GRCGYC 1 cut(s) 337
BstBI TTCGAA 1 cut(s) 1215
BstC8I GCNNGC 6 cut(s) 53, 322, 523, 760, 764, 1260
BstDEI CTNAG 5 cut(s) 104, 863, 885, 1067, 1286
BstDSI CCRYGG 2 cut(s) 332, 466
BstF5I GGATG 6 cut(s) 384, 510, 754, 1177, 1234, 1258
BstFNI CGCG 2 cut(s) 449, 1011
BstH2I RGCGCY 2 cut(s) 340, 1166
BstHHI GCGC 5 cut(s) 339, 880, 1003, 1013, 1165
BstKTI GATC 2 cut(s) 91, 950
BstMAI GTCTC 1 cut(s) 188
BstMBI GATC 2 cut(s) 88, 947
BstNI CCWGG 1 cut(s) 832
BstNSI RCATGY 1 cut(s) 355
BstSCI CCNGG 3 cut(s) 525, 830, 1275
BstUI CGCG 2 cut(s) 449, 1011
BstV1I GCAGC 7 cut(s) 38, 60, 255, 618, 1050, 1072, 1152
BsuRI GGCC 5 cut(s) 5, 492, 521, 694, 766
BtgI CCRYGG 2 cut(s) 332, 466
BtsCI GGATG 6 cut(s) 384, 510, 754, 1177, 1234, 1258
BtsIMutI CAGTG 1 cut(s) 780
BveI ACCTGC 1 cut(s) 108
Cac8I GCNNGC 6 cut(s) 53, 322, 523, 760, 764, 1260
CaiI CAGNNNCTG 1 cut(s) 1066
CfoI GCGC 5 cut(s) 339, 880, 1003, 1013, 1165
Cfr10I RCCGGY 1 cut(s) 320
Cfr13I GGNCC 5 cut(s) 490, 570, 588, 692, 848
CpoI CGGWCCG 1 cut(s) 588
Csp6I GTAC 1 cut(s) 889
CspI CGGWCCG 1 cut(s) 588
CviAII CATG 1 cut(s) 352
CviQI GTAC 1 cut(s) 889
DdeI CTNAG 5 cut(s) 104, 863, 885, 1067, 1286
DinI GGCGCC 1 cut(s) 338
DpnI GATC 2 cut(s) 90, 949
DpnII GATC 2 cut(s) 88, 947
EaeI YGGCCR 2 cut(s) 3, 764
Eam1104I CTCTTC 2 cut(s) 954, 1080
EarI CTCTTC 2 cut(s) 954, 1080
EciI GGCGGA 8 cut(s) 36, 69, 109, 199, 208, 240, 287, 530
Ecl136II GAGCTC 1 cut(s) 583
Eco130I CCWWGG 1 cut(s) 923
Eco147I AGGCCT 1 cut(s) 521
Eco24I GRGCYC 2 cut(s) 585, 1054
Eco31I GGTCTC 1 cut(s) 188
Eco47I GGWCC 3 cut(s) 570, 588, 848
Eco53kI GAGCTC 1 cut(s) 583
Eco57I CTGAAG 2 cut(s) 753, 1190
EcoICRI GAGCTC 1 cut(s) 583
EcoO109I RGGNCCY 1 cut(s) 570
EcoRII CCWGG 1 cut(s) 830
EcoT14I CCWWGG 1 cut(s) 923
EcoT38I GRGCYC 2 cut(s) 585, 1054
EgeI GGCGCC 1 cut(s) 338
EheI GGCGCC 1 cut(s) 338
ErhI CCWWGG 1 cut(s) 923
FaeI CATG 1 cut(s) 355
FaiI YATR 6 cut(s) 353, 596, 858, 893, 942, 1005
FaqI GGGAC 3 cut(s) 583, 914, 1042
FatI CATG 1 cut(s) 351
FauI CCCGC 1 cut(s) 325
FokI GGATG 6 cut(s) 371, 517, 761, 1184, 1241, 1265
FriOI GRGCYC 2 cut(s) 585, 1054
FspBI CTAG 1 cut(s) 191
GlaI GCGC 5 cut(s) 338, 879, 1002, 1012, 1164
GsaI CCCAGC 1 cut(s) 692
HaeII RGCGCY 2 cut(s) 340, 1166
HaeIII GGCC 5 cut(s) 5, 492, 521, 694, 766
HapII CCGG 7 cut(s) 321, 526, 586, 782, 796, 1169, 1276
HhaI GCGC 5 cut(s) 339, 880, 1003, 1013, 1165
Hin1I GRCGYC 1 cut(s) 337
Hin1II CATG 1 cut(s) 355
Hin6I GCGC 5 cut(s) 337, 878, 1001, 1011, 1163
HinP1I GCGC 5 cut(s) 337, 878, 1001, 1011, 1163
HinfI GANTC 6 cut(s) 326, 421, 509, 728, 827, 934
HpaII CCGG 7 cut(s) 321, 526, 586, 782, 796, 1169, 1276
HphI GGTGA 4 cut(s) 106, 139, 692, 1057
Hpy188I TCNGA 6 cut(s) 358, 402, 592, 603, 733, 1146
Hpy188III TCNNGA 3 cut(s) 284, 502, 746
HpyAV CCTTC 7 cut(s) 151, 386, 426, 728, 979, 1075, 1211
HpyCH4III ACNGT 2 cut(s) 775, 905
HpyCH4V TGCA 1 cut(s) 546
HpyF3I CTNAG 5 cut(s) 104, 863, 885, 1067, 1286
Hsp92I GRCGYC 1 cut(s) 337
Hsp92II CATG 1 cut(s) 355
HspAI GCGC 5 cut(s) 337, 878, 1001, 1011, 1163
KasI GGCGCC 1 cut(s) 336
KroI GCCGGC 1 cut(s) 320
KroNI GCCGGC 1 cut(s) 322
Kzo9I GATC 2 cut(s) 88, 947
LmnI GCTCC 4 cut(s) 23, 470, 588, 1201
Lsp1109I GCAGC 7 cut(s) 38, 60, 255, 618, 1050, 1072, 1152
MaeI CTAG 1 cut(s) 191
MaeIII GTNAC 2 cut(s) 112, 1117
MalI GATC 2 cut(s) 90, 949
MbiI CCGCTC 1 cut(s) 85
MboI GATC 2 cut(s) 88, 947
MboII GAAGA 3 cut(s) 70, 971, 1097
MhlI GDGCHC 3 cut(s) 585, 773, 1054
MluCI AATT 3 cut(s) 563, 637, 1138
Mly113I GGCGCC 1 cut(s) 337
MlyI GAGTC 4 cut(s) 320, 503, 836, 928
MreI CGCCGGCG 1 cut(s) 320
MroNI GCCGGC 1 cut(s) 320
MseI TTAA 1 cut(s) 993
MspA1I CMGCKG 3 cut(s) 15, 268, 1063
MspI CCGG 7 cut(s) 321, 526, 586, 782, 796, 1169, 1276
MspR9I CCNGG 3 cut(s) 527, 832, 1277
Mva1269I GAATGC 1 cut(s) 293
MvaI CCWGG 1 cut(s) 832
MvnI CGCG 2 cut(s) 449, 1011
NaeI GCCGGC 1 cut(s) 322
NarI GGCGCC 1 cut(s) 337
NciI CCSGG 2 cut(s) 527, 1277
NdeII GATC 2 cut(s) 88, 947
NgoMIV GCCGGC 1 cut(s) 320
NlaIII CATG 1 cut(s) 355
NlaIV GGNNCC 5 cut(s) 296, 338, 472, 571, 693
NmeAIII GCCGAG 3 cut(s) 491, 792, 977
NmuCI GTSAC 1 cut(s) 112
NspI RCATGY 1 cut(s) 355
NspV TTCGAA 1 cut(s) 1215
PceI AGGCCT 1 cut(s) 521
PcsI WCGNNNNNNNCGW 1 cut(s) 1037
PctI GAATGC 1 cut(s) 293
PdiI GCCGGC 1 cut(s) 322
PfeI GAWTC 2 cut(s) 421, 728
PleI GAGTC 4 cut(s) 320, 503, 835, 928
PluTI GGCGCC 1 cut(s) 340
PpsI GAGTC 4 cut(s) 320, 503, 835, 928
PpuMI RGGWCCY 1 cut(s) 570
Psp124BI GAGCTC 1 cut(s) 585
Psp5II RGGWCCY 1 cut(s) 570
Psp6I CCWGG 1 cut(s) 830
PspFI CCCAGC 1 cut(s) 688
PspGI CCWGG 1 cut(s) 830
PspN4I GGNNCC 5 cut(s) 296, 338, 472, 571, 693
PspPI GGNCC 5 cut(s) 490, 570, 588, 692, 848
PspPPI RGGWCCY 1 cut(s) 570
PstNI CAGNNNCTG 1 cut(s) 1066
PvuII CAGCTG 1 cut(s) 1063
RsaI GTAC 1 cut(s) 890
RsaNI GTAC 1 cut(s) 889
Rsr2I CGGWCCG 1 cut(s) 588
RsrII CGGWCCG 1 cut(s) 588
SacI GAGCTC 1 cut(s) 585
SaqAI TTAA 1 cut(s) 993
Sau3AI GATC 2 cut(s) 88, 947
Sau96I GGNCC 5 cut(s) 490, 570, 588, 692, 848
SchI GAGTC 4 cut(s) 320, 503, 836, 928
ScrFI CCNGG 3 cut(s) 527, 832, 1277
SduI GDGCHC 3 cut(s) 585, 773, 1054
SfoI GGCGCC 1 cut(s) 338
SfuI TTCGAA 1 cut(s) 1215
SgrAI CRCCGGYG 1 cut(s) 320
SinI GGWCC 3 cut(s) 570, 588, 848
SmlI CTYRAG 3 cut(s) 282, 1189, 1270
SmoI CTYRAG 3 cut(s) 282, 1189, 1270
Sse9I AATT 3 cut(s) 563, 637, 1138
SseBI AGGCCT 1 cut(s) 521
SspDI GGCGCC 1 cut(s) 336
SspMI CTAG 1 cut(s) 191
SstI GAGCTC 1 cut(s) 585
StuI AGGCCT 1 cut(s) 521
StyD4I CCNGG 3 cut(s) 525, 830, 1275
StyI CCWWGG 1 cut(s) 923
TaaI ACNGT 2 cut(s) 775, 905
TaqI TCGA 4 cut(s) 87, 419, 972, 1215
TasI AATT 3 cut(s) 563, 637, 1138
TatI WGTACW 1 cut(s) 888
TauI GCSGC 9 cut(s) 8, 18, 21, 85, 303, 446, 449, 495, 498
TfiI GAWTC 2 cut(s) 421, 728
Tru1I TTAA 1 cut(s) 993
Tru9I TTAA 1 cut(s) 993
TscAI CASTG 1 cut(s) 780
TseFI GTSAC 1 cut(s) 112
TseI GCWGC 7 cut(s) 26, 48, 268, 631, 1060, 1063, 1165
Tsp45I GTSAC 1 cut(s) 112
TspDTI ATGAA 1 cut(s) 1163
TspGWI ACGGA 2 cut(s) 483, 927
TspRI CASTG 1 cut(s) 780
VpaK11BI GGWCC 3 cut(s) 570, 588, 848
XceI RCATGY 1 cut(s) 355
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.