RchiOBHm_Chr2g0087021

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0009592
Physical Location & Seq
Reverse (-)
1938453 .. 1939495
1043 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46249

Sequence Viewer

Length: 792 bp
ATGGACTCGGCGGAGGCCTGCCGGGCTTTCTACGCCGTCTGCAAGTCGGTCAATGATAAATTAGGGAGCTTGTTTGAGCTCCGGTCCGATATGGCTTGGAGTGTTGGGGGTTTCGGGTGGATTTCGCTGCCGAATTGCTGTGGGGTGTTAGGGTTTGGGAGATTTGGGGTTAGTGGTGAAATGCTGGGGCCAAATGGGTCGCTGTTGGTTCAGGCATTGGTGGATTCTGAAGGGAGGTTTCTGGATGTTTCGGCAGGCTGGCCGAGCACAGTGAAACCGGAAGCGATATTCCGGCAGAGTAAGCTGTATTTGGGTGTGGAGGAGTCCAGGGAGTTGTTGAATGGTCCTGTTTATGAGCTCAGTGATGGCAATGCGCTTCCTCAGTACATACTGGGGGACTCTTGCTTTCCTCTGTTGCCTTGGCTTTTGACTCCGTATGTGAGATCAAATGAGGAAGAGGAGAGTTTCGGTACATTGGAGAAGGCGTTTAACTCGGCGCATGGTGGCGCAATGGGGCTGGTTGGGACGGCGTTTGCGAGGGTGAGGGCTCGGTGGCAGCTGCTGAGTAGGGAGTGGAAGGAAGAGTGTGTGGAGTTTTTGCCGTTTGTGGTGGTTACTGGCTGTCTGCTGAATAATTTTCTGATGAAGTGTAGTGAGCCGCTGCCGGATGACAATGTGGGGTGCTTGAGGGAGGAGGAGCTTCAGGTGTTCGAAGGACAGGTGGATGAGAGTGGCGAGAGGATTAGGGATGTGCTTGCCACGCACTTGAGCCGGGTAAGCCTAAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

263

Amino Acids

29.06

Weight (kDa)

4.76

Isoelectric Point (pI)

49.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 67 - 212 7.8e-16 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 11, 659
AcoI YGGCCR 1 cut(s) 260
AcuI CTGAAG 2 cut(s) 249, 686
AfaI GTAC 2 cut(s) 386, 472
AgsI TTSAA 1 cut(s) 340
AjnI CCWGG 1 cut(s) 326
AluBI AGCT 6 cut(s) 69, 79, 304, 358, 559, 700
AluI AGCT 6 cut(s) 69, 79, 304, 358, 559, 700
Alw21I GWGCWC 3 cut(s) 81, 269, 360
AlwNI CAGNNNCTG 1 cut(s) 562
AoxI GGCC 3 cut(s) 15, 188, 260
ApeKI GCWGC 4 cut(s) 127, 556, 559, 661
AspLEI GCGC 3 cut(s) 376, 499, 509
AspS9I GGNCC 3 cut(s) 84, 188, 344
AsuC2I CCSGG 2 cut(s) 23, 773
AsuHPI GGTGA 2 cut(s) 188, 553
AsuII TTCGAA 1 cut(s) 711
AvaII GGWCC 2 cut(s) 84, 344
BanII GRGCYC 3 cut(s) 81, 360, 550
Bbv12I GWGCWC 3 cut(s) 81, 269, 360
BbvI GCAGC 4 cut(s) 114, 546, 568, 648
BccI CCATC 1 cut(s) 359
BceAI ACGGC 3 cut(s) 20, 543, 586
BciT130I CCWGG 1 cut(s) 328
BcnI CCSGG 2 cut(s) 23, 773
BglI GCCNNNNNGGC 1 cut(s) 23
BisI GCNGC 5 cut(s) 128, 557, 560, 659, 662
BlsI GCNGC 5 cut(s) 129, 558, 561, 660, 663
Bme1390I CCNGG 3 cut(s) 23, 328, 773
Bme18I GGWCC 2 cut(s) 84, 344
BmgT120I GGNCC 3 cut(s) 84, 188, 344
BmiI GGNNCC 1 cut(s) 189
BmrFI CCNGG 3 cut(s) 23, 328, 773
BmrI ACTGGG 1 cut(s) 401
BmuI ACTGGG 1 cut(s) 401
Bpu14I TTCGAA 1 cut(s) 711
BpuEI CTTGAG 2 cut(s) 706, 787
BpuMI CCSGG 2 cut(s) 23, 773
BsaJI CCNNGG 2 cut(s) 327, 419
BsaWI WCCGGW 2 cut(s) 81, 277
BsaXI ACNNNNNCTCC 2 cut(s) 470, 500
Bse1I ACTGG 2 cut(s) 396, 622
Bse3DI GCAATG 2 cut(s) 376, 516
BseBI CCWGG 1 cut(s) 328
BseDI CCNNGG 2 cut(s) 327, 419
BseGI GGATG 4 cut(s) 250, 673, 730, 754
BseMI GCAATG 2 cut(s) 376, 516
BseMII CTCAG 3 cut(s) 373, 395, 554
BseNI ACTGG 2 cut(s) 396, 622
BseRI GAGGAG 4 cut(s) 335, 473, 707, 710
BseXI GCAGC 4 cut(s) 114, 546, 568, 648
BseYI CCCAGC 1 cut(s) 184
BshFI GGCC 3 cut(s) 17, 190, 262
BsiHKAI GWGCWC 3 cut(s) 81, 269, 360
BsiSI CCGG 6 cut(s) 22, 82, 278, 292, 665, 772
BslFI GGGAC 2 cut(s) 410, 538
BsmFI GGGAC 2 cut(s) 410, 538
BsnI GGCC 3 cut(s) 17, 190, 262
Bsp119I TTCGAA 1 cut(s) 711
Bsp1286I GDGCHC 4 cut(s) 81, 269, 360, 550
Bsp143I GATC 1 cut(s) 443
BspACI CCGC 2 cut(s) 11, 659
BspANI GGCC 3 cut(s) 17, 190, 262
BspCNI CTCAG 3 cut(s) 372, 394, 555
BspLI GGNNCC 1 cut(s) 189
BspT104I TTCGAA 1 cut(s) 711
BsrDI GCAATG 2 cut(s) 376, 516
BsrI ACTGG 2 cut(s) 396, 622
BssECI CCNNGG 2 cut(s) 327, 419
BssMI GATC 1 cut(s) 443
BssT1I CCWWGG 1 cut(s) 419
Bst2UI CCWGG 1 cut(s) 328
Bst4CI ACNGT 1 cut(s) 271
Bst6I CTCTTC 2 cut(s) 450, 576
BstBI TTCGAA 1 cut(s) 711
BstC8I GCNNGC 4 cut(s) 19, 256, 260, 756
BstDEI CTNAG 4 cut(s) 359, 381, 563, 782
BstF5I GGATG 4 cut(s) 250, 673, 730, 754
BstHHI GCGC 3 cut(s) 376, 499, 509
BstKTI GATC 1 cut(s) 446
BstMBI GATC 1 cut(s) 443
BstMWI GCNNNNNNNGC 6 cut(s) 23, 32, 264, 301, 760, 777
BstNI CCWGG 1 cut(s) 328
BstSCI CCNGG 3 cut(s) 21, 326, 771
BstV1I GCAGC 4 cut(s) 114, 546, 568, 648
BsuRI GGCC 3 cut(s) 17, 190, 262
BtsCI GGATG 4 cut(s) 250, 673, 730, 754
BtsIMutI CAGTG 2 cut(s) 276, 367
Cac8I GCNNGC 4 cut(s) 19, 256, 260, 756
CaiI CAGNNNCTG 1 cut(s) 562
CfoI GCGC 3 cut(s) 376, 499, 509
Cfr13I GGNCC 3 cut(s) 84, 188, 344
CpoI CGGWCCG 1 cut(s) 84
Csp6I GTAC 2 cut(s) 385, 471
CspI CGGWCCG 1 cut(s) 84
CviAII CATG 1 cut(s) 500
CviQI GTAC 2 cut(s) 385, 471
DdeI CTNAG 4 cut(s) 359, 381, 563, 782
DpnI GATC 1 cut(s) 445
DpnII GATC 1 cut(s) 443
EaeI YGGCCR 1 cut(s) 260
Eam1104I CTCTTC 2 cut(s) 450, 576
EarI CTCTTC 2 cut(s) 450, 576
EciI GGCGGA 1 cut(s) 26
Ecl136II GAGCTC 2 cut(s) 79, 358
Eco130I CCWWGG 1 cut(s) 419
Eco147I AGGCCT 1 cut(s) 17
Eco24I GRGCYC 3 cut(s) 81, 360, 550
Eco47I GGWCC 2 cut(s) 84, 344
Eco53kI GAGCTC 2 cut(s) 79, 358
Eco57I CTGAAG 2 cut(s) 249, 686
EcoICRI GAGCTC 2 cut(s) 79, 358
EcoRII CCWGG 1 cut(s) 326
EcoT14I CCWWGG 1 cut(s) 419
EcoT38I GRGCYC 3 cut(s) 81, 360, 550
ErhI CCWWGG 1 cut(s) 419
FaeI CATG 1 cut(s) 503
FaiI YATR 5 cut(s) 92, 354, 389, 438, 501
FaqI GGGAC 2 cut(s) 410, 538
FatI CATG 1 cut(s) 499
Fnu4HI GCNGC 5 cut(s) 128, 557, 560, 659, 662
FokI GGATG 4 cut(s) 257, 680, 737, 761
FriOI GRGCYC 3 cut(s) 81, 360, 550
Fsp4HI GCNGC 5 cut(s) 128, 557, 560, 659, 662
GlaI GCGC 3 cut(s) 375, 498, 508
GluI GCNGC 5 cut(s) 128, 557, 560, 659, 662
GsaI CCCAGC 1 cut(s) 188
HaeIII GGCC 3 cut(s) 17, 190, 262
HapII CCGG 6 cut(s) 22, 82, 278, 292, 665, 772
HhaI GCGC 3 cut(s) 376, 499, 509
Hin1II CATG 1 cut(s) 503
Hin6I GCGC 3 cut(s) 374, 497, 507
HinP1I GCGC 3 cut(s) 374, 497, 507
HinfI GANTC 5 cut(s) 5, 224, 323, 398, 430
HpaII CCGG 6 cut(s) 22, 82, 278, 292, 665, 772
HphI GGTGA 2 cut(s) 188, 553
Hpy188I TCNGA 3 cut(s) 88, 229, 642
Hpy188III TCNNGA 1 cut(s) 242
HpyAV CCTTC 4 cut(s) 224, 475, 571, 707
HpyCH4III ACNGT 1 cut(s) 271
HpyCH4V TGCA 1 cut(s) 42
HpyF10VI GCNNNNNNNGC 6 cut(s) 23, 32, 264, 301, 760, 777
HpyF3I CTNAG 4 cut(s) 359, 381, 563, 782
Hsp92II CATG 1 cut(s) 503
HspAI GCGC 3 cut(s) 374, 497, 507
Kzo9I GATC 1 cut(s) 443
LmnI GCTCC 3 cut(s) 66, 84, 697
Lsp1109I GCAGC 4 cut(s) 114, 546, 568, 648
MaeIII GTNAC 1 cut(s) 613
MalI GATC 1 cut(s) 445
MboI GATC 1 cut(s) 443
MboII GAAGA 2 cut(s) 467, 593
MhlI GDGCHC 4 cut(s) 81, 269, 360, 550
MluCI AATT 3 cut(s) 59, 133, 634
MlyI GAGTC 3 cut(s) 332, 392, 424
MseI TTAA 1 cut(s) 489
MspA1I CMGCKG 2 cut(s) 559, 661
MspI CCGG 6 cut(s) 22, 82, 278, 292, 665, 772
MspR9I CCNGG 3 cut(s) 23, 328, 773
MvaI CCWGG 1 cut(s) 328
MwoI GCNNNNNNNGC 6 cut(s) 23, 32, 264, 301, 760, 777
NciI CCSGG 2 cut(s) 23, 773
NdeII GATC 1 cut(s) 443
NlaIII CATG 1 cut(s) 503
NlaIV GGNNCC 1 cut(s) 189
NmeAIII GCCGAG 2 cut(s) 288, 473
NspV TTCGAA 1 cut(s) 711
PceI AGGCCT 1 cut(s) 17
PcsI WCGNNNNNNNCGW 1 cut(s) 533
PfeI GAWTC 1 cut(s) 224
PkrI GCNGC 5 cut(s) 129, 558, 561, 660, 663
PleI GAGTC 3 cut(s) 331, 392, 424
PpsI GAGTC 3 cut(s) 331, 392, 424
Psp124BI GAGCTC 2 cut(s) 81, 360
Psp6I CCWGG 1 cut(s) 326
PspFI CCCAGC 1 cut(s) 184
PspGI CCWGG 1 cut(s) 326
PspN4I GGNNCC 1 cut(s) 189
PspPI GGNCC 3 cut(s) 84, 188, 344
PstNI CAGNNNCTG 1 cut(s) 562
PvuII CAGCTG 1 cut(s) 559
RsaI GTAC 2 cut(s) 386, 472
RsaNI GTAC 2 cut(s) 385, 471
Rsr2I CGGWCCG 1 cut(s) 84
RsrII CGGWCCG 1 cut(s) 84
SacI GAGCTC 2 cut(s) 81, 360
SaqAI TTAA 1 cut(s) 489
SatI GCNGC 5 cut(s) 128, 557, 560, 659, 662
Sau3AI GATC 1 cut(s) 443
Sau96I GGNCC 3 cut(s) 84, 188, 344
SchI GAGTC 3 cut(s) 332, 392, 424
ScrFI CCNGG 3 cut(s) 23, 328, 773
SduI GDGCHC 4 cut(s) 81, 269, 360, 550
SetI ASST 9 cut(s) 71, 81, 239, 306, 360, 561, 702, 708, 723
SfuI TTCGAA 1 cut(s) 711
SinI GGWCC 2 cut(s) 84, 344
SmlI CTYRAG 2 cut(s) 685, 766
SmoI CTYRAG 2 cut(s) 685, 766
Sse9I AATT 3 cut(s) 59, 133, 634
SseBI AGGCCT 1 cut(s) 17
SsiI CCGC 2 cut(s) 11, 659
SstI GAGCTC 2 cut(s) 81, 360
StuI AGGCCT 1 cut(s) 17
StyD4I CCNGG 3 cut(s) 21, 326, 771
StyI CCWWGG 1 cut(s) 419
TaaI ACNGT 1 cut(s) 271
TaqI TCGA 1 cut(s) 711
TaqII GACCGA 1 cut(s) 37
TasI AATT 3 cut(s) 59, 133, 634
TatI WGTACW 1 cut(s) 384
TauI GCSGC 1 cut(s) 661
TfiI GAWTC 1 cut(s) 224
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TscAI CASTG 2 cut(s) 276, 367
TseI GCWGC 4 cut(s) 127, 556, 559, 661
TspDTI ATGAA 1 cut(s) 659
TspGWI ACGGA 1 cut(s) 423
TspRI CASTG 2 cut(s) 276, 367
VpaK11BI GGWCC 2 cut(s) 84, 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.