FvH4_1g03370

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0004338
Physical Location & Seq
Reverse (-)
1863182 .. 1865957
2776 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g03370.t1

Sequence Viewer

Length: 1740 bp
ATGGAGGAGAAAATGAGTTGGACTGCGGCGGATGCTGTGGACTACAAAGGGTTTCCTGCTGATAAGTCCAGAACTGGTGGTTGGGTTTCCGCAGCGCTTATACTAGGAATTGAAATATGTGAAAGGCTATCAACTATGGGAATAGCAGTGAACCTGGTGACGTACCTAGTTGGGACAATGCATTTGCCAAGCTCAACTTCAGCAAATATTGTCACAGACTTTGCAGGCACATCCTTTCTTCTCTGTTTGCTAGGAGGATTCTTAGCCGATTCCTTCCTTGGCAGATACAAGACCATTGCAATCTTTGCTTCAATACAGTCATTGGGCATAGTTGCACTAGCAATATCAGTAAAATTGCCGCAGCTGCGCCCACCTCATTGTGACATTACTGCTGCCAACAACGACTGCCAACAAGCCAATGAGTTCCAAATGGGATTTCTTTACTTGGCTTTATATATGATTGCATTAGGGATTGGCGGGCTGAAATCAAGTGTTTCAGGATTTGGAACAGATCAATTTGATGACAAAGATGACAAAGAGAAAGCGCAGATGGCCTATTTCTTTAATAGATTTTTCTTTTTCATTAGCACTGGAACCTTGATGGCAGTCACAGTCCTTGTTTACATACAAGACCAAGTGGACAGAAGCTTGGCCTATGGAATATGTTCCATTTCCATGTTCATAGCCATCTTGGTGTTTTTCTCGGGGACTAAAAGATACAGATATAAGAAAAGCACAGGAAGTCCCATAGTTCAGATTTTTCAGGTTATTGTGGCTGCTGTAAGGAAGAGAAAGATGCCACTTCCATTCGACGTTGCCTTGTTGTATGAGAATTATCCTGAAGCTTCAAGAATTTGTCACACTGATCAGTTCCGTTTCTTGGACAAGGCAGCTATTGTGGCAGAAGAAGATTTTGAGAGAAATGCACCTGCAGGTTCTCTTCCGAACCCATGGAAGTTATGCTCAGTTACAAGAGTGGAGGAGGTGAAAATGATGGTGAAACTACTTCCAATATGGGCCACAACCATCATGTTTTGGACAGCATATGCACAAATGCTCACCTTCTCAGTAGAACAAGCATCTACCATGGAAAGGTCCATCGGAAAGTTTCAAATCCCAGCTGGCTCTCTCACAGTCTTCTTTGTGTTAGCCATATTGATCACACTGGCTGTTAATGACCGTATCATTATGCCTGTTTGGAAGAAATGGAAAGGCCAACCAGGTTTCACCAATCTACAGAGAATTGCAATAGGCCTTGTCCTATCGACTCTGGGAATGGCAGCGGCAGCACTAACTGAGAAGAAGCGGTTATTAGTGGCTAAAGGCATGAAAACTATGAGTACAGGTGCTCTTCCTATTAGTGTGTACTGGTTAATCCCACAGTTCTTCCTTGTGGGTTCTGGTGAAGCTTTCATCTACACTGGCCAGCTCGATTTCTTCATAACTCAATCACCAAAAGGAATGAAAACTATGAGCACAGGGCTCTTCCTGAGCACCATAGCACTAGGCTTCTTTGTCAGTAGCTTCTTGGTTTCGATCGTGAGGAGGTTCACTGGAAGCAAAGATGGGCAAGGATGGCTTGCAGACAATATCAACTATGGAAGACTAGACTGTTTCTATGGACTTTTGAGCGTTTTAAGTGTCATAAATTTTGTAGCATATCTTGTTTGTGCAAGGTGGTACAAGCCAAGAAAACCTACCAAACCATCTAATGGTTCCTCTGCTGAGGATAAGTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0006950 GO:0008150 GO:0009611 GO:0009719 GO:0009725 GO:0009753 GO:0010033 GO:0042221 GO:0050896 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

580

Amino Acids

64.02

Weight (kDa)

9.19

Isoelectric Point (pI)

34.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 96 - 526 2.7e-101 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 937
Acc36I ACCTGC 2 cut(s) 923, 937
AccB7I CCANNNNNTGG 1 cut(s) 1712
AciI CCGC 7 cut(s) 26, 29, 90, 359, 477, 1283, 1306
AcoI YGGCCR 1 cut(s) 1423
AcsI RAATTY 2 cut(s) 852, 1648
AcuI CTGAAG 2 cut(s) 183, 861
AfaI GTAC 4 cut(s) 164, 1342, 1367, 1682
AfeI AGCGCT 1 cut(s) 96
AfiI CCNNNNNNNGG 3 cut(s) 880, 1092, 1712
AgsI TTSAA 4 cut(s) 113, 312, 849, 1112
AjnI CCWGG 2 cut(s) 153, 1219
AjuI GAANNNNNNNTTGG 6 cut(s) 64, 96, 181, 213, 420, 452
AluBI AGCT 9 cut(s) 192, 364, 648, 845, 893, 1121, 1409, 1429, 1524
AluI AGCT 9 cut(s) 192, 364, 648, 845, 893, 1121, 1409, 1429, 1524
Alw21I GWGCWC 3 cut(s) 1351, 1478, 1496
Ama87I CYCGRG 1 cut(s) 703
Aor51HI AGCGCT 1 cut(s) 96
AoxI GGCC 6 cut(s) 552, 651, 1017, 1213, 1252, 1423
ApeKI GCWGC 8 cut(s) 92, 361, 364, 392, 776, 890, 1280, 1286
ApoI RAATTY 2 cut(s) 852, 1648
ArsI GACNNNNNNTTYG 2 cut(s) 166, 198
Asp700I GAANNNNTTC 1 cut(s) 664
AspLEI GCGC 3 cut(s) 97, 369, 547
AspS9I GGNCC 2 cut(s) 1017, 1095
AsuHPI GGTGA 7 cut(s) 169, 997, 1009, 1051, 1219, 1415, 1443
AvaI CYCGRG 1 cut(s) 703
AvaII GGWCC 1 cut(s) 1095
BalI TGGCCA 1 cut(s) 1425
BanII GRGCYC 1 cut(s) 1485
BbsI GAAGAC 2 cut(s) 1129, 1609
Bbv12I GWGCWC 3 cut(s) 1351, 1478, 1496
BbvCI CCTCAGC 1 cut(s) 1725
BbvI GCAGC 8 cut(s) 104, 351, 373, 379, 763, 902, 1292, 1298
BccI CCATC 9 cut(s) 544, 595, 695, 988, 1034, 1106, 1559, 1569, 1714
BciT130I CCWGG 2 cut(s) 155, 1221
BclI TGATCA 2 cut(s) 865, 1158
BfaI CTAG 6 cut(s) 104, 167, 251, 338, 1505, 1607
BfmI CTRYAG 2 cut(s) 930, 1235
BfoI RGCGCY 1 cut(s) 98
BfuAI ACCTGC 2 cut(s) 923, 937
Bme1390I CCNGG 2 cut(s) 155, 1221
Bme18I GGWCC 1 cut(s) 1095
BmeT110I CYCGRG 1 cut(s) 703
BmgT120I GGNCC 2 cut(s) 1017, 1095
BmiI GGNNCC 2 cut(s) 595, 1717
BmrFI CCNGG 2 cut(s) 155, 1221
BmsI GCATC 3 cut(s) 22, 786, 1088
BpiI GAAGAC 2 cut(s) 1129, 1609
Bpu10I CCTNAGC 2 cut(s) 1490, 1725
BsaJI CCNNGG 3 cut(s) 277, 950, 1086
Bsc4I CCNNNNNNNGG 3 cut(s) 880, 1092, 1712
Bse1I ACTGG 6 cut(s) 79, 595, 1170, 1373, 1426, 1558
Bse3DI GCAATG 1 cut(s) 294
BseBI CCWGG 2 cut(s) 155, 1221
BseDI CCNNGG 3 cut(s) 277, 950, 1086
BseGI GGATG 3 cut(s) 37, 230, 1580
BseLI CCNNNNNNNGG 3 cut(s) 880, 1092, 1712
BseMI GCAATG 1 cut(s) 294
BseMII CTCAG 5 cut(s) 978, 1080, 1287, 1481, 1716
BseNI ACTGG 6 cut(s) 79, 595, 1170, 1373, 1426, 1558
BseRI GAGGAG 3 cut(s) 20, 995, 1558
BseXI GCAGC 8 cut(s) 104, 351, 373, 379, 763, 902, 1292, 1298
BseYI CCCAGC 1 cut(s) 1117
Bsh1285I CGRYCG 1 cut(s) 1539
BshFI GGCC 6 cut(s) 554, 653, 1019, 1215, 1254, 1425
BsiEI CGRYCG 1 cut(s) 1539
BsiHKAI GWGCWC 3 cut(s) 1351, 1478, 1496
BsiHKCI CYCGRG 1 cut(s) 703
BslFI GGGAC 3 cut(s) 187, 721, 729
BslI CCNNNNNNNGG 3 cut(s) 880, 1092, 1712
BsmFI GGGAC 3 cut(s) 187, 721, 729
BsnI GGCC 6 cut(s) 554, 653, 1019, 1215, 1254, 1425
BsoBI CYCGRG 1 cut(s) 703
Bsp1286I GDGCHC 4 cut(s) 1351, 1478, 1485, 1496
Bsp143I GATC 4 cut(s) 511, 865, 1158, 1536
Bsp19I CCATGG 2 cut(s) 950, 1086
BspACI CCGC 7 cut(s) 26, 29, 90, 359, 477, 1283, 1306
BspANI GGCC 6 cut(s) 554, 653, 1019, 1215, 1254, 1425
BspCNI CTCAG 5 cut(s) 977, 1079, 1288, 1482, 1717
BspLI GGNNCC 2 cut(s) 595, 1717
BspMAI CTGCAG 1 cut(s) 934
BspMI ACCTGC 2 cut(s) 923, 937
BspQI GCTCTTC 2 cut(s) 1356, 1490
BsrDI GCAATG 1 cut(s) 294
BsrI ACTGG 6 cut(s) 79, 595, 1170, 1373, 1426, 1558
BssECI CCNNGG 3 cut(s) 277, 950, 1086
BssMI GATC 4 cut(s) 511, 865, 1158, 1536
BssT1I CCWWGG 3 cut(s) 277, 950, 1086
Bst2UI CCWGG 2 cut(s) 155, 1221
Bst4CI ACNGT 6 cut(s) 318, 613, 1135, 1181, 1383, 1613
Bst6I CTCTTC 4 cut(s) 782, 945, 1356, 1490
BstAPI GCANNNNNTGC 1 cut(s) 305
BstC8I GCNNGC 5 cut(s) 226, 479, 1123, 1427, 1581
BstDEI CTNAG 6 cut(s) 262, 964, 1066, 1296, 1490, 1725
BstDSI CCRYGG 2 cut(s) 950, 1086
BstF5I GGATG 3 cut(s) 37, 230, 1580
BstH2I RGCGCY 1 cut(s) 98
BstHHI GCGC 3 cut(s) 97, 369, 547
BstKTI GATC 4 cut(s) 514, 868, 1161, 1539
BstMBI GATC 4 cut(s) 511, 865, 1158, 1536
BstMCI CGRYCG 1 cut(s) 1539
BstMWI GCNNNNNNNGC 7 cut(s) 32, 305, 364, 551, 899, 1286, 1576
BstNI CCWGG 2 cut(s) 155, 1221
BstSCI CCNGG 2 cut(s) 153, 1219
BstSFI CTRYAG 2 cut(s) 930, 1235
BstV1I GCAGC 8 cut(s) 104, 351, 373, 379, 763, 902, 1292, 1298
BstV2I GAAGAC 2 cut(s) 1129, 1609
BsuRI GGCC 6 cut(s) 554, 653, 1019, 1215, 1254, 1425
BtgI CCRYGG 2 cut(s) 950, 1086
BtsCI GGATG 3 cut(s) 37, 230, 1580
BtsI GCAGTG 1 cut(s) 153
BtsIMutI CAGTG 6 cut(s) 153, 588, 861, 1163, 1419, 1551
BveI ACCTGC 2 cut(s) 923, 937
Cac8I GCNNGC 5 cut(s) 226, 479, 1123, 1427, 1581
CfoI GCGC 3 cut(s) 97, 369, 547
Cfr13I GGNCC 2 cut(s) 1017, 1095
CsiI ACCWGGT 2 cut(s) 153, 1219
Csp6I GTAC 4 cut(s) 163, 1341, 1366, 1681
CviAII CATG 5 cut(s) 676, 951, 1030, 1087, 1327
CviQI GTAC 4 cut(s) 163, 1341, 1366, 1681
DdeI CTNAG 6 cut(s) 262, 964, 1066, 1296, 1490, 1725
DpnI GATC 4 cut(s) 513, 867, 1160, 1538
DpnII GATC 4 cut(s) 511, 865, 1158, 1536
EaeI YGGCCR 1 cut(s) 1423
Eam1104I CTCTTC 4 cut(s) 782, 945, 1356, 1490
EarI CTCTTC 4 cut(s) 782, 945, 1356, 1490
EciI GGCGGA 1 cut(s) 44
Eco130I CCWWGG 3 cut(s) 277, 950, 1086
Eco147I AGGCCT 1 cut(s) 1254
Eco24I GRGCYC 1 cut(s) 1485
Eco47I GGWCC 1 cut(s) 1095
Eco47III AGCGCT 1 cut(s) 96
Eco57I CTGAAG 2 cut(s) 183, 861
Eco88I CYCGRG 1 cut(s) 703
EcoRII CCWGG 2 cut(s) 153, 1219
EcoT14I CCWWGG 3 cut(s) 277, 950, 1086
EcoT22I ATGCAT 1 cut(s) 183
EcoT38I GRGCYC 1 cut(s) 1485
ErhI CCWWGG 3 cut(s) 277, 950, 1086
FaeI CATG 5 cut(s) 679, 954, 1033, 1090, 1330
FalI AAGNNNNNCTT 4 cut(s) 181, 213, 1563, 1595
FaqI GGGAC 3 cut(s) 187, 721, 729
FatI CATG 5 cut(s) 675, 950, 1029, 1086, 1326
FauI CCCGC 1 cut(s) 470
FauNDI CATATG 1 cut(s) 1045
FbaI TGATCA 2 cut(s) 865, 1158
FokI GGATG 3 cut(s) 44, 217, 1587
FriOI GRGCYC 1 cut(s) 1485
FspBI CTAG 6 cut(s) 104, 167, 251, 338, 1505, 1607
GlaI GCGC 3 cut(s) 96, 368, 546
GsaI CCCAGC 1 cut(s) 1121
HaeII RGCGCY 1 cut(s) 98
HaeIII GGCC 6 cut(s) 554, 653, 1019, 1215, 1254, 1425
HhaI GCGC 3 cut(s) 97, 369, 547
Hin1II CATG 5 cut(s) 679, 954, 1033, 1090, 1330
Hin6I GCGC 3 cut(s) 95, 367, 545
HinP1I GCGC 3 cut(s) 95, 367, 545
HindIII AAGCTT 3 cut(s) 646, 843, 1407
HinfI GANTC 3 cut(s) 258, 269, 1267
HphI GGTGA 7 cut(s) 169, 997, 1009, 1051, 1219, 1415, 1443
Hpy166II GTNNAC 6 cut(s) 40, 151, 622, 640, 1366, 1551
Hpy188I TCNGA 3 cut(s) 756, 945, 1103
Hpy188III TCNNGA 6 cut(s) 69, 498, 839, 849, 1489, 1540
Hpy8I GTNNAC 6 cut(s) 40, 151, 622, 640, 1366, 1551
Hpy99I CGWCG 1 cut(s) 815
HpyAV CCTTC 2 cut(s) 283, 1072
HpyCH4III ACNGT 6 cut(s) 318, 613, 1135, 1181, 1383, 1613
HpyCH4IV ACGT 2 cut(s) 161, 813
HpyF10VI GCNNNNNNNGC 7 cut(s) 32, 305, 364, 551, 899, 1286, 1576
HpyF3I CTNAG 6 cut(s) 262, 964, 1066, 1296, 1490, 1725
HpySE526I ACGT 2 cut(s) 161, 813
Hsp92II CATG 5 cut(s) 679, 954, 1033, 1090, 1330
HspAI GCGC 3 cut(s) 95, 367, 545
Ksp22I TGATCA 2 cut(s) 865, 1158
Kzo9I GATC 4 cut(s) 511, 865, 1158, 1536
LguI GCTCTTC 2 cut(s) 1356, 1490
Lsp1109I GCAGC 8 cut(s) 104, 351, 373, 379, 763, 902, 1292, 1298
LweI GCATC 3 cut(s) 22, 786, 1088
MabI ACCWGGT 2 cut(s) 153, 1219
MaeI CTAG 6 cut(s) 104, 167, 251, 338, 1505, 1607
MaeII ACGT 2 cut(s) 161, 813
MaeIII GTNAC 6 cut(s) 157, 211, 380, 607, 857, 967
MalI GATC 4 cut(s) 513, 867, 1160, 1538
MboI GATC 4 cut(s) 511, 865, 1158, 1536
MhlI GDGCHC 4 cut(s) 1351, 1478, 1485, 1496
MlsI TGGCCA 1 cut(s) 1425
MluCI AATT 7 cut(s) 108, 353, 515, 832, 852, 1242, 1648
MluNI TGGCCA 1 cut(s) 1425
MlyI GAGTC 1 cut(s) 1261
MnlI CCTC 8 cut(s) 248, 384, 973, 976, 1536, 1539, 1720, 1729
Mox20I TGGCCA 1 cut(s) 1425
Mph1103I ATGCAT 1 cut(s) 183
MroXI GAANNNNTTC 1 cut(s) 664
MscI TGGCCA 1 cut(s) 1425
MseI TTAA 4 cut(s) 564, 1173, 1373, 1637
MslI CAYNNNNRTG 2 cut(s) 674, 692
Msp20I TGGCCA 1 cut(s) 1425
MspA1I CMGCKG 3 cut(s) 364, 1121, 1283
MspR9I CCNGG 2 cut(s) 155, 1221
MvaI CCWGG 2 cut(s) 155, 1221
MwoI GCNNNNNNNGC 7 cut(s) 32, 305, 364, 551, 899, 1286, 1576
NcoI CCATGG 2 cut(s) 950, 1086
NdeI CATATG 1 cut(s) 1045
NdeII GATC 4 cut(s) 511, 865, 1158, 1536
NlaIII CATG 5 cut(s) 679, 954, 1033, 1090, 1330
NlaIV GGNNCC 2 cut(s) 595, 1717
NmuCI GTSAC 5 cut(s) 157, 211, 380, 607, 857
NsiI ATGCAT 1 cut(s) 183
PaqCI CACCTGC 1 cut(s) 937
PceI AGGCCT 1 cut(s) 1254
PciSI GCTCTTC 2 cut(s) 1356, 1490
PdmI GAANNNNTTC 1 cut(s) 664
PfeI GAWTC 2 cut(s) 258, 269
PflMI CCANNNNNTGG 1 cut(s) 1712
Ple19I CGATCG 1 cut(s) 1539
PleI GAGTC 1 cut(s) 1261
PpsI GAGTC 1 cut(s) 1261
Psp6I CCWGG 2 cut(s) 153, 1219
PspFI CCCAGC 1 cut(s) 1117
PspGI CCWGG 2 cut(s) 153, 1219
PspN4I GGNNCC 2 cut(s) 595, 1717
PspPI GGNCC 2 cut(s) 1017, 1095
PstI CTGCAG 1 cut(s) 934
PvuI CGATCG 1 cut(s) 1539
PvuII CAGCTG 2 cut(s) 364, 1121
RsaI GTAC 4 cut(s) 164, 1342, 1367, 1682
RsaNI GTAC 4 cut(s) 163, 1341, 1366, 1681
RseI CAYNNNNRTG 2 cut(s) 674, 692
SapI GCTCTTC 2 cut(s) 1356, 1490
SaqAI TTAA 4 cut(s) 564, 1173, 1373, 1637
Sau3AI GATC 4 cut(s) 511, 865, 1158, 1536
Sau96I GGNCC 2 cut(s) 1017, 1095
SbfI CCTGCAGG 1 cut(s) 934
SchI GAGTC 1 cut(s) 1261
ScrFI CCNGG 2 cut(s) 155, 1221
SdaI CCTGCAGG 1 cut(s) 934
SduI GDGCHC 4 cut(s) 1351, 1478, 1485, 1496
SexAI ACCWGGT 2 cut(s) 153, 1219
SfaNI GCATC 3 cut(s) 22, 786, 1088
SfcI CTRYAG 2 cut(s) 930, 1235
SinI GGWCC 1 cut(s) 1095
SmiMI CAYNNNNRTG 2 cut(s) 674, 692
Sse8387I CCTGCAGG 1 cut(s) 934
Sse9I AATT 7 cut(s) 108, 353, 515, 832, 852, 1242, 1648
SseBI AGGCCT 1 cut(s) 1254
SsiI CCGC 7 cut(s) 26, 29, 90, 359, 477, 1283, 1306
SspI AATATT 1 cut(s) 208
SspMI CTAG 6 cut(s) 104, 167, 251, 338, 1505, 1607
StuI AGGCCT 1 cut(s) 1254
StyD4I CCNGG 2 cut(s) 153, 1219
StyI CCWWGG 3 cut(s) 277, 950, 1086
TaaI ACNGT 6 cut(s) 318, 613, 1135, 1181, 1383, 1613
TaiI ACGT 2 cut(s) 164, 816
TaqI TCGA 4 cut(s) 810, 1265, 1431, 1535
TasI AATT 7 cut(s) 108, 353, 515, 832, 852, 1242, 1648
TatI WGTACW 2 cut(s) 1340, 1365
TauI GCSGC 3 cut(s) 29, 361, 1286
TfiI GAWTC 2 cut(s) 258, 269
Tru1I TTAA 4 cut(s) 564, 1173, 1373, 1637
Tru9I TTAA 4 cut(s) 564, 1173, 1373, 1637
TscAI CASTG 6 cut(s) 153, 595, 868, 1170, 1426, 1558
TseFI GTSAC 5 cut(s) 157, 211, 380, 607, 857
TseI GCWGC 8 cut(s) 92, 361, 364, 392, 776, 890, 1280, 1286
Tsp45I GTSAC 5 cut(s) 157, 211, 380, 607, 857
TspDTI ATGAA 6 cut(s) 571, 670, 1343, 1402, 1429, 1478
TspGWI ACGGA 1 cut(s) 863
TspRI CASTG 6 cut(s) 153, 595, 868, 1170, 1426, 1558
Van91I CCANNNNNTGG 1 cut(s) 1712
VpaK11BI GGWCC 1 cut(s) 1095
XapI RAATTY 2 cut(s) 852, 1648
XmnI GAANNNNTTC 1 cut(s) 664
XspI CTAG 6 cut(s) 104, 167, 251, 338, 1505, 1607
Zsp2I ATGCAT 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.