Rroxscaffold_2G00152500

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004338
Physical Location & Seq
Forward (+)
89444408 .. 89447428
3021 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00152500.1

Sequence Viewer

Length: 1803 bp
ATGGAGGAGAAAATGAGTGGGTCTGCGGCCGATGCTCTGGACTACAAAGGGCTTCCTGCTGATAAGTCCAGAACTGGTGGTTGGGTTGCCGCGGCGCTTATACTAGGAATTGAAATATGTGAACGGCTATCGACTATGGGAATAGCAGTGAACCTGGTGACATACCTAGTTGGGACAATGCATTTGCCAAGCTCAACTTCAGCAAATATTGTCACAGACTTTGCAGGCACATCCTTCCTCCTTTGTTTGCTTGGAGGCTTCCTCGCCGATTCCTTCCTTGGCAGATACAAGACCATTGCAATCTTTGCTTCAATACAGACATTGGGCATAATTGCATTAGCAATATCAGTAGAATTGCCACAGCTGCGGCCACCTCATTGTGACATTACTGCTGCCAACAACAACTGCAAACAAGCCAATGACTTCCAAACGGGATTCCTTTACTTGGCTTTATATATAATTGCACTAGGGATTGGTGGGCTGAAATCAAGTGTTTCCGGATTTGGAACTGATCAATTTGATGAAAAAGATGACAAAGAGAAAGCGCAGATGGCATATTTCTTCAATAGATTTTTCTTTTTCATTAGCACTGGAACCTTGATGGCAGTCACAGTCCTTGTCTACATACAAGACCAAGTGGACAGAAGCTGGGCCTATGGAATATGTTCCATTTCAATGTTCATAGCCATCTTGGTGTTTTTTTCGGGGACTAAAAGATACAGATATAAGAAAAGCTTGGGAAGTCCCATAGTTCAAATTTTTCAGGTTATTGTGGCTGCTGTAAGGAAGAGAAAGATGCCACTTCCATTCGACGTTGCCTTGTTGTATGAGAATTATCCCGAGGCTTCAAGAATTGGTCACACTGATCAGTTCCTTTGCTATCCATGTAACTCATTATCGTATAGTGCTGATGCTCATCTGGTTCAATTTTTGGTTAGTTTCTTGGACAAGGCAGCTATTGTGGCAGAAGAAGATTTTGCGAAAAATGCACCTGCAGGTTCTGTTCCGAACCCATGGAATTTATGCTCAGTTACAAGAGTGGAGGAGGTGAAAATGATGGTGAAACTACTTCCGATATGGGCCACAACCATCATGTTTTGGACAGCATACGCACAGATGCTCACCTTCTCAGTAGAACAAGCATCCACCATGGAGAGATCCATTGGAAAGTTTCAAATCCCGGCTGGCTCTCTCACAGCCTTCTTTGTGTTAGCCATATTGATCACATTGGCTGTTAATGACCGTCTCATTATGCCTATTTGGAAGAAATGGAAGGGCCAACCAGGTTTCACCAATCTACAGAGAATTGCAATTGGCCTTGTCCTATCGACTCTTGGAATGGCAGCGGCAGCACTAACCGAGAGGAAGCGGTTATCAGTGGCTAAAGAAATGAGAACTATGAGCACAGGTCCTCTGCCTATTAGTGTGTACTTGTTAATCCCACAATTCTTCTTGGTGGGTTCTGGTGAAGCTTTCATCTACACTGGCCAGCTCGATTTCTTTATAACTCAATCACCAAAAGGAATGAAAACTATGAGCACAGGACTCTTCCTGAGCACTTTATCACTAGGCTTCTTTGTCAGTAGCTTCTTGGTTTCAATTGTGAGGAGGTTCACCGGAAGCAAAGATGGGCAAGGATGGCTTGCAGACAATATCAACTATGGAAGGCTTGACTGTTTCTATGGACTTTTGAGCGTTTTAAGTGTCATAAATTTTGTAGCATATCTTGTTTGTGCAAGATGGTACAAGCCAAGAAAACCTACCAAACCATCCAACGGTTCCTCTGCTGAGGATAGGTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0006950 GO:0008150 GO:0009611 GO:0009719 GO:0009725 GO:0009753 GO:0010033 GO:0042221 GO:0050896 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

600

Amino Acids

66.09

Weight (kDa)

8.99

Isoelectric Point (pI)

31.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 96 - 547 5.5e-100 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1505
AarI CACCTGC 1 cut(s) 1000
Acc36I ACCTGC 2 cut(s) 986, 1000
AccI GTMKAC 1 cut(s) 621
AccII CGCG 1 cut(s) 92
AccIII TCCGGA 1 cut(s) 497
AciI CCGC 6 cut(s) 26, 90, 92, 367, 1346, 1369
AclWI GGATC 1 cut(s) 1152
AcoI YGGCCR 3 cut(s) 27, 368, 1486
AcsI RAATTY 3 cut(s) 756, 1018, 1711
AcuI CTGAAG 1 cut(s) 183
AfaI GTAC 2 cut(s) 1430, 1745
AfiI CCNNNNNNNGG 2 cut(s) 445, 1775
AgsI TTSAA 9 cut(s) 113, 312, 565, 675, 755, 849, 926, 1175, 1599
AjnI CCWGG 2 cut(s) 153, 1282
AjuI GAANNNNNNNTTGG 4 cut(s) 64, 96, 181, 213
AluBI AGCT 8 cut(s) 192, 364, 648, 735, 956, 1472, 1492, 1587
AluI AGCT 8 cut(s) 192, 364, 648, 735, 956, 1472, 1492, 1587
Alw21I GWGCWC 3 cut(s) 1406, 1541, 1559
Alw26I GTCTC 1 cut(s) 1250
AlwI GGATC 1 cut(s) 1152
AlwNI CAGNNNCTG 2 cut(s) 648, 1001
Ama87I CYCGRG 1 cut(s) 839
Aor13HI TCCGGA 1 cut(s) 497
AoxI GGCC 7 cut(s) 27, 368, 651, 1080, 1276, 1315, 1486
ApeKI GCWGC 6 cut(s) 364, 392, 776, 953, 1343, 1349
ApoI RAATTY 3 cut(s) 756, 1018, 1711
ArsI GACNNNNNNTTYG 2 cut(s) 166, 198
Asp700I GAANNNNTTC 1 cut(s) 664
AspLEI GCGC 2 cut(s) 97, 547
AspS9I GGNCC 4 cut(s) 651, 1080, 1276, 1409
AsuC2I CCSGG 1 cut(s) 1181
AsuHPI GGTGA 8 cut(s) 169, 1060, 1072, 1114, 1282, 1478, 1506, 1606
AvaI CYCGRG 1 cut(s) 839
AvaII GGWCC 1 cut(s) 1409
BalI TGGCCA 1 cut(s) 1488
Bbv12I GWGCWC 3 cut(s) 1406, 1541, 1559
BbvCI CCTCAGC 1 cut(s) 1788
BbvI GCAGC 6 cut(s) 351, 379, 763, 965, 1355, 1361
BccI CCATC 9 cut(s) 544, 595, 695, 1051, 1097, 1622, 1632, 1734, 1777
BceAI ACGGC 1 cut(s) 140
BciT130I CCWGG 2 cut(s) 155, 1284
BclI TGATCA 3 cut(s) 511, 865, 1221
BcnI CCSGG 1 cut(s) 1181
BcoDI GTCTC 1 cut(s) 1250
BfaI CTAG 4 cut(s) 104, 167, 467, 1568
BfmI CTRYAG 2 cut(s) 993, 1298
BfoI RGCGCY 1 cut(s) 98
BfuAI ACCTGC 2 cut(s) 986, 1000
Bme1390I CCNGG 3 cut(s) 155, 1181, 1284
Bme18I GGWCC 1 cut(s) 1409
BmeT110I CYCGRG 1 cut(s) 839
BmgT120I GGNCC 4 cut(s) 651, 1080, 1276, 1409
BmiI GGNNCC 2 cut(s) 595, 1780
BmrFI CCNGG 3 cut(s) 155, 1181, 1284
BmsI GCATC 5 cut(s) 22, 786, 901, 1107, 1151
BplI GAGNNNNNCTC 2 cut(s) 246, 278
Bpu10I CCTNAGC 2 cut(s) 1553, 1788
BpuMI CCSGG 1 cut(s) 1181
BsaBI GATNNNNATC 1 cut(s) 915
BsaJI CCNNGG 5 cut(s) 90, 277, 840, 1013, 1149
BsaWI WCCGGW 2 cut(s) 497, 1616
Bsc4I CCNNNNNNNGG 2 cut(s) 445, 1775
Bse1I ACTGG 3 cut(s) 79, 595, 1489
Bse3DI GCAATG 1 cut(s) 294
Bse8I GATNNNNATC 1 cut(s) 915
BseAI TCCGGA 1 cut(s) 497
BseBI CCWGG 2 cut(s) 155, 1284
BseDI CCNNGG 5 cut(s) 90, 277, 840, 1013, 1149
BseGI GGATG 4 cut(s) 230, 1142, 1643, 1769
BseJI GATNNNNATC 1 cut(s) 915
BseLI CCNNNNNNNGG 2 cut(s) 445, 1775
BseMI GCAATG 1 cut(s) 294
BseMII CTCAG 4 cut(s) 1041, 1143, 1544, 1779
BseNI ACTGG 3 cut(s) 79, 595, 1489
BseRI GAGGAG 3 cut(s) 20, 1058, 1621
BseX3I CGGCCG 1 cut(s) 27
BseXI GCAGC 6 cut(s) 351, 379, 763, 965, 1355, 1361
BseYI CCCAGC 1 cut(s) 648
Bsh1236I CGCG 1 cut(s) 92
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 7 cut(s) 29, 370, 653, 1082, 1278, 1317, 1488
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 3 cut(s) 1406, 1541, 1559
BsiHKCI CYCGRG 1 cut(s) 839
BsiSI CCGG 3 cut(s) 498, 1181, 1617
BslFI GGGAC 3 cut(s) 187, 721, 729
BslI CCNNNNNNNGG 2 cut(s) 445, 1775
BsmAI GTCTC 1 cut(s) 1250
BsmBI CGTCTC 1 cut(s) 1250
BsmFI GGGAC 3 cut(s) 187, 721, 729
BsnI GGCC 7 cut(s) 29, 370, 653, 1082, 1278, 1317, 1488
BsoBI CYCGRG 1 cut(s) 839
Bsp1286I GDGCHC 3 cut(s) 1406, 1541, 1559
Bsp13I TCCGGA 1 cut(s) 497
Bsp143I GATC 4 cut(s) 511, 865, 1157, 1221
Bsp19I CCATGG 2 cut(s) 1013, 1149
BspACI CCGC 6 cut(s) 26, 90, 92, 367, 1346, 1369
BspANI GGCC 7 cut(s) 29, 370, 653, 1082, 1278, 1317, 1488
BspCNI CTCAG 4 cut(s) 1040, 1142, 1545, 1780
BspEI TCCGGA 1 cut(s) 497
BspFNI CGCG 1 cut(s) 92
BspLI GGNNCC 2 cut(s) 595, 1780
BspMAI CTGCAG 1 cut(s) 997
BspMI ACCTGC 2 cut(s) 986, 1000
BspPI GGATC 1 cut(s) 1152
BsrDI GCAATG 1 cut(s) 294
BsrI ACTGG 3 cut(s) 79, 595, 1489
BssECI CCNNGG 5 cut(s) 90, 277, 840, 1013, 1149
BssMI GATC 4 cut(s) 511, 865, 1157, 1221
BssT1I CCWWGG 3 cut(s) 277, 1013, 1149
Bst2UI CCWGG 2 cut(s) 155, 1284
Bst4CI ACNGT 4 cut(s) 613, 1244, 1676, 1778
Bst6I CTCTTC 2 cut(s) 782, 1553
BstAPI GCANNNNNTGC 1 cut(s) 305
BstC8I GCNNGC 4 cut(s) 226, 1186, 1490, 1644
BstDEI CTNAG 4 cut(s) 1027, 1129, 1553, 1788
BstDSI CCRYGG 3 cut(s) 90, 1013, 1149
BstF5I GGATG 4 cut(s) 230, 1142, 1643, 1769
BstFNI CGCG 1 cut(s) 92
BstH2I RGCGCY 1 cut(s) 98
BstHHI GCGC 2 cut(s) 97, 547
BstKTI GATC 4 cut(s) 514, 868, 1160, 1224
BstMAI GTCTC 1 cut(s) 1250
BstMBI GATC 4 cut(s) 511, 865, 1157, 1221
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 8 cut(s) 32, 305, 364, 551, 962, 986, 1349, 1639
BstNI CCWGG 2 cut(s) 155, 1284
BstSCI CCNGG 3 cut(s) 153, 1179, 1282
BstSFI CTRYAG 2 cut(s) 993, 1298
BstUI CGCG 1 cut(s) 92
BstV1I GCAGC 6 cut(s) 351, 379, 763, 965, 1355, 1361
BstX2I RGATCY 1 cut(s) 1157
BstYI RGATCY 1 cut(s) 1157
BstZI CGGCCG 1 cut(s) 27
BsuRI GGCC 7 cut(s) 29, 370, 653, 1082, 1278, 1317, 1488
BtgI CCRYGG 3 cut(s) 90, 1013, 1149
BtsCI GGATG 4 cut(s) 230, 1142, 1643, 1769
BtsI GCAGTG 1 cut(s) 153
BtsIMutI CAGTG 5 cut(s) 153, 588, 861, 1383, 1482
BveI ACCTGC 2 cut(s) 986, 1000
Cac8I GCNNGC 4 cut(s) 226, 1186, 1490, 1644
CaiI CAGNNNCTG 2 cut(s) 648, 1001
CfoI GCGC 2 cut(s) 97, 547
Cfr13I GGNCC 4 cut(s) 651, 1080, 1276, 1409
Cfr42I CCGCGG 1 cut(s) 93
CsiI ACCWGGT 2 cut(s) 153, 1282
Csp6I GTAC 2 cut(s) 1429, 1744
CviAII CATG 4 cut(s) 885, 1014, 1093, 1150
CviQI GTAC 2 cut(s) 1429, 1744
DdeI CTNAG 4 cut(s) 1027, 1129, 1553, 1788
DpnI GATC 4 cut(s) 513, 867, 1159, 1223
DpnII GATC 4 cut(s) 511, 865, 1157, 1221
EaeI YGGCCR 3 cut(s) 27, 368, 1486
EagI CGGCCG 1 cut(s) 27
Eam1104I CTCTTC 2 cut(s) 782, 1553
EarI CTCTTC 2 cut(s) 782, 1553
EclXI CGGCCG 1 cut(s) 27
Eco130I CCWWGG 3 cut(s) 277, 1013, 1149
Eco47I GGWCC 1 cut(s) 1409
Eco52I CGGCCG 1 cut(s) 27
Eco57I CTGAAG 1 cut(s) 183
Eco88I CYCGRG 1 cut(s) 839
EcoO109I RGGNCCY 1 cut(s) 1409
EcoRII CCWGG 2 cut(s) 153, 1282
EcoT14I CCWWGG 3 cut(s) 277, 1013, 1149
EcoT22I ATGCAT 1 cut(s) 183
ErhI CCWWGG 3 cut(s) 277, 1013, 1149
Esp3I CGTCTC 1 cut(s) 1250
FaeI CATG 4 cut(s) 888, 1017, 1096, 1153
FalI AAGNNNNNCTT 6 cut(s) 181, 213, 719, 751, 1626, 1658
FaqI GGGAC 3 cut(s) 187, 721, 729
FatI CATG 4 cut(s) 884, 1013, 1092, 1149
FbaI TGATCA 3 cut(s) 511, 865, 1221
FblI GTMKAC 1 cut(s) 621
FokI GGATG 4 cut(s) 217, 1129, 1650, 1756
FspBI CTAG 4 cut(s) 104, 167, 467, 1568
GlaI GCGC 2 cut(s) 96, 546
GsaI CCCAGC 1 cut(s) 652
HaeII RGCGCY 1 cut(s) 98
HaeIII GGCC 7 cut(s) 29, 370, 653, 1082, 1278, 1317, 1488
HapII CCGG 3 cut(s) 498, 1181, 1617
HhaI GCGC 2 cut(s) 97, 547
Hin1II CATG 4 cut(s) 888, 1017, 1096, 1153
Hin6I GCGC 2 cut(s) 95, 545
HinP1I GCGC 2 cut(s) 95, 545
HindIII AAGCTT 2 cut(s) 733, 1470
HinfI GANTC 4 cut(s) 269, 435, 1330, 1545
HpaII CCGG 3 cut(s) 498, 1181, 1617
HphI GGTGA 8 cut(s) 169, 1060, 1072, 1114, 1282, 1478, 1506, 1606
Hpy166II GTNNAC 6 cut(s) 122, 151, 622, 640, 1429, 1614
Hpy188I TCNGA 2 cut(s) 1008, 1074
Hpy188III TCNNGA 6 cut(s) 38, 69, 498, 839, 849, 1552
Hpy8I GTNNAC 6 cut(s) 122, 151, 622, 640, 1429, 1614
Hpy99I CGWCG 1 cut(s) 815
HpyAV CCTTC 6 cut(s) 244, 283, 1135, 1210, 1267, 1659
HpyCH4III ACNGT 4 cut(s) 613, 1244, 1676, 1778
HpyCH4IV ACGT 1 cut(s) 813
HpyF10VI GCNNNNNNNGC 8 cut(s) 32, 305, 364, 551, 962, 986, 1349, 1639
HpyF3I CTNAG 4 cut(s) 1027, 1129, 1553, 1788
HpySE526I ACGT 1 cut(s) 813
Hsp92II CATG 4 cut(s) 888, 1017, 1096, 1153
HspAI GCGC 2 cut(s) 95, 545
Kpn2I TCCGGA 1 cut(s) 497
Ksp22I TGATCA 3 cut(s) 511, 865, 1221
KspI CCGCGG 1 cut(s) 93
Kzo9I GATC 4 cut(s) 511, 865, 1157, 1221
Lsp1109I GCAGC 6 cut(s) 351, 379, 763, 965, 1355, 1361
LweI GCATC 5 cut(s) 22, 786, 901, 1107, 1151
MabI ACCWGGT 2 cut(s) 153, 1282
MaeI CTAG 4 cut(s) 104, 167, 467, 1568
MaeII ACGT 1 cut(s) 813
MaeIII GTNAC 7 cut(s) 157, 211, 380, 607, 857, 887, 1030
MalI GATC 4 cut(s) 513, 867, 1159, 1223
MboI GATC 4 cut(s) 511, 865, 1157, 1221
MboII GAAGA 7 cut(s) 553, 799, 980, 983, 1276, 1441, 1540
MfeI CAATTG 2 cut(s) 1311, 1599
MflI RGATCY 1 cut(s) 1157
MhlI GDGCHC 3 cut(s) 1406, 1541, 1559
MlsI TGGCCA 1 cut(s) 1488
MluNI TGGCCA 1 cut(s) 1488
MlyI GAGTC 2 cut(s) 1324, 1539
MmeI TCCRAC 1 cut(s) 1797
Mox20I TGGCCA 1 cut(s) 1488
Mph1103I ATGCAT 1 cut(s) 183
MroI TCCGGA 1 cut(s) 497
MroXI GAANNNNTTC 1 cut(s) 664
MscI TGGCCA 1 cut(s) 1488
MseI TTAA 3 cut(s) 1236, 1436, 1700
MslI CAYNNNNRTG 2 cut(s) 674, 692
Msp20I TGGCCA 1 cut(s) 1488
MspA1I CMGCKG 3 cut(s) 92, 364, 1346
MspI CCGG 3 cut(s) 498, 1181, 1617
MspR9I CCNGG 3 cut(s) 155, 1181, 1284
MunI CAATTG 2 cut(s) 1311, 1599
MvaI CCWGG 2 cut(s) 155, 1284
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 8 cut(s) 32, 305, 364, 551, 962, 986, 1349, 1639
NciI CCSGG 1 cut(s) 1181
NcoI CCATGG 2 cut(s) 1013, 1149
NdeII GATC 4 cut(s) 511, 865, 1157, 1221
NlaIII CATG 4 cut(s) 888, 1017, 1096, 1153
NlaIV GGNNCC 2 cut(s) 595, 1780
NmuCI GTSAC 5 cut(s) 157, 211, 380, 607, 857
NsiI ATGCAT 1 cut(s) 183
PaqCI CACCTGC 1 cut(s) 1000
PdmI GAANNNNTTC 1 cut(s) 664
PfeI GAWTC 2 cut(s) 269, 435
PleI GAGTC 2 cut(s) 1324, 1539
PpsI GAGTC 2 cut(s) 1324, 1539
PpuMI RGGWCCY 1 cut(s) 1409
PsiI TTATAA 1 cut(s) 1505
Psp5II RGGWCCY 1 cut(s) 1409
Psp6I CCWGG 2 cut(s) 153, 1282
PspFI CCCAGC 1 cut(s) 648
PspGI CCWGG 2 cut(s) 153, 1282
PspN4I GGNNCC 2 cut(s) 595, 1780
PspPI GGNCC 4 cut(s) 651, 1080, 1276, 1409
PspPPI RGGWCCY 1 cut(s) 1409
PstI CTGCAG 1 cut(s) 997
PstNI CAGNNNCTG 2 cut(s) 648, 1001
PsuI RGATCY 1 cut(s) 1157
PvuII CAGCTG 1 cut(s) 364
RsaI GTAC 2 cut(s) 1430, 1745
RsaNI GTAC 2 cut(s) 1429, 1744
RseI CAYNNNNRTG 2 cut(s) 674, 692
SacII CCGCGG 1 cut(s) 93
SaqAI TTAA 3 cut(s) 1236, 1436, 1700
Sau3AI GATC 4 cut(s) 511, 865, 1157, 1221
Sau96I GGNCC 4 cut(s) 651, 1080, 1276, 1409
SbfI CCTGCAGG 1 cut(s) 997
SchI GAGTC 2 cut(s) 1324, 1539
ScrFI CCNGG 3 cut(s) 155, 1181, 1284
SdaI CCTGCAGG 1 cut(s) 997
SduI GDGCHC 3 cut(s) 1406, 1541, 1559
SexAI ACCWGGT 2 cut(s) 153, 1282
SfaNI GCATC 5 cut(s) 22, 786, 901, 1107, 1151
SfcI CTRYAG 2 cut(s) 993, 1298
Sfr303I CCGCGG 1 cut(s) 93
SgrBI CCGCGG 1 cut(s) 93
SinI GGWCC 1 cut(s) 1409
SmiMI CAYNNNNRTG 2 cut(s) 674, 692
Sse8387I CCTGCAGG 1 cut(s) 997
SsiI CCGC 6 cut(s) 26, 90, 92, 367, 1346, 1369
SspI AATATT 1 cut(s) 208
SspMI CTAG 4 cut(s) 104, 167, 467, 1568
StyD4I CCNGG 3 cut(s) 153, 1179, 1282
StyI CCWWGG 3 cut(s) 277, 1013, 1149
TaaI ACNGT 4 cut(s) 613, 1244, 1676, 1778
TaiI ACGT 1 cut(s) 816
TaqI TCGA 4 cut(s) 131, 810, 1328, 1494
TatI WGTACW 1 cut(s) 1428
TauI GCSGC 5 cut(s) 29, 92, 95, 370, 1349
TfiI GAWTC 2 cut(s) 269, 435
Tru1I TTAA 3 cut(s) 1236, 1436, 1700
Tru9I TTAA 3 cut(s) 1236, 1436, 1700
TscAI CASTG 5 cut(s) 153, 595, 868, 1383, 1489
TseFI GTSAC 5 cut(s) 157, 211, 380, 607, 857
TseI GCWGC 6 cut(s) 364, 392, 776, 953, 1343, 1349
Tsp45I GTSAC 5 cut(s) 157, 211, 380, 607, 857
TspDTI ATGAA 5 cut(s) 537, 571, 670, 1465, 1541
TspRI CASTG 5 cut(s) 153, 595, 868, 1383, 1489
VpaK11BI GGWCC 1 cut(s) 1409
XapI RAATTY 3 cut(s) 756, 1018, 1711
XmiI GTMKAC 1 cut(s) 621
XmnI GAANNNNTTC 1 cut(s) 664
XspI CTAG 4 cut(s) 104, 167, 467, 1568
Zsp2I ATGCAT 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.