Rw2G003350

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004338
Physical Location & Seq
Forward (+)
3142307 .. 3144865
2559 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G003350.1

Sequence Viewer

Length: 1704 bp
ATGGAGGAGAAAACGAGTTGGACTGCGGCCGATGCTGTGGACTACAAAGGGTCTTCTGCAGATAAGTCCATAACTGGTGGTTGGGTTACTGCCACGCTTATATTAGGAATTGAAATATGTGAAAGGCTATCAACTATGGGAATAGCAGTGAACCTGGTGACGTACCTGGTTGGGACAATGCATTTGCCAAGCTCAACTTCAGCAAATATTGTCACAGACTTTTTAGGCACATCCTTCCTCCTTTGTTTGCTTGGAGGGTTCCTCGCCGATTCCTTCCTTGGCAGATACAAGACCATTGCAATCTTTGCTTCAATACAGACATTGGGCACTGCTGCATTAGCAATATCAGTAAAATTGCCGCAGCTGCGTCCACCTCATTGTGATATTACTGCTACCAACAATTGCAAACAAGCCAATAAGCTCCAAATGGGAATTCTTTACTTGGCTTTATATATAATTGCATTAGGGACTGGTGGGTTGAAATCAAGTGTTTCAGGATTTGGAACTGATCAATTTGATGAAAAAGATGACAGAGAGAAAGCGCAGATGGCATATTTCTTCAATAGATTTTTCTTCTTCATTAGCACAGGATCCTTGATGGCAGTCACAGTCCTTGTCTACATACAAGACCAAGTGGACAGAAGCTGGGCCTATGGAATATGTTCCATTTCAATGTTTATAGCCATCTTGCTGTTTTTTTTTGGGACTAAAAGATACAGATATAAGAAAAGTTTGGGAAGTCCCATAGTTCAAATTTTTCAGGTTATTGTGGCTGCTATGAGGAAGAGAAAGATGCCACTTCCATTCGACGTTGCCTTGTTGTATGAGAATTATCCCGAGGCTTCAAGAACTGGTCACACAGATCAGTTCCGTTTCTTGGACAAGGCATCTATTGTGGCAGAAGAAGATTCCCAGAGAAATGAACCAGCAGGTTCTGTTCCGAACCCATGGAAGTTATGCTCAGTTACAAGAGTGGAGGAGGTGAAAATGATGGTGAAACTACTTCCGATATGGGCCACAACCATCATCTTTTGGACAACATACGGACAGATGATGACCTTCTCAGTAGAACAAGCATCCACCATGGAGAGATCCGTCGGAAAGTTCCAGATCCCGGCTGGCTCTCTCACGGTCTTCTTTGTGCTGGCCATACTGATCACACTGGCTGTTAATGACCGTCTCATTATGCCTATTTGGAAGAAATGGAAGGGCCAGCCAGGTTTCACCGATCTACAGAGAATTGCAATTGGCCTTGTTCTATCGACTCTTGGAATGGCAGTGGCAGCACTAACCGAGAGGAAGCGGTTATCAGTGGCTAAAGAAATGAGAACTACGAGTGCAGTTTCTCTGCCTATTGGTGTGTTCTTGTTAATCCCACAATTCTTCCTAGTAGCTCGATTTTTTATAACTCAATCACCAAAAGGAATGAAAACTATGAGCACAGGACTCTTCCTGAGCACTTTATCACTAGGCTTCTTTTTCAGTAGCTTCTTGGTTTCAATTGTGAGGAGGTTCACCGGAAGCAAAAATGGGCAAGGATGGCTTGTAGACAATATCAACTATGGAAGGCTTGACTGTTTCTATGGACTTTTGAGCGTTTTAAGTGTCATTAATTTTGCAGCATATCTCGTTTGTGCAAGATGGTACAAACCAAGGAAATCTACCAAACCATCTGACGGTTCCTCTGCTGAGGGTAGGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0006950 GO:0008150 GO:0009611 GO:0009719 GO:0009725 GO:0009753 GO:0010033 GO:0042221 GO:0050896 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

567

Amino Acids

62.85

Weight (kDa)

9.48

Isoelectric Point (pI)

31.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 96 - 515 8e-94 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1406
Acc36I ACCTGC 1 cut(s) 920
AccI GTMKAC 2 cut(s) 618, 1548
AciI CCGC 3 cut(s) 26, 359, 1303
AclWI GGATC 4 cut(s) 585, 598, 1086, 1105
AcoI YGGCCR 2 cut(s) 27, 1146
AcsI RAATTY 2 cut(s) 432, 753
AcuI CTGAAG 1 cut(s) 183
AfaI GTAC 2 cut(s) 164, 1646
AfiI CCNNNNNNNGG 3 cut(s) 877, 1114, 1676
AgsI TTSAA 8 cut(s) 113, 312, 481, 562, 672, 752, 846, 1500
AjnI CCWGG 3 cut(s) 153, 165, 1216
AjuI GAANNNNNNNTTGG 4 cut(s) 181, 213, 417, 449
AluBI AGCT 6 cut(s) 192, 364, 421, 645, 1394, 1488
AluI AGCT 6 cut(s) 192, 364, 421, 645, 1394, 1488
Alw21I GWGCWC 2 cut(s) 1442, 1460
Alw26I GTCTC 1 cut(s) 1184
AlwI GGATC 4 cut(s) 585, 598, 1086, 1105
AlwNI CAGNNNCTG 2 cut(s) 645, 935
Ama87I CYCGRG 1 cut(s) 836
AoxI GGCC 6 cut(s) 27, 648, 1014, 1146, 1210, 1249
ApeKI GCWGC 6 cut(s) 332, 361, 364, 773, 1283, 1619
ApoI RAATTY 2 cut(s) 432, 753
ArsI GACNNNNNNTTYG 2 cut(s) 166, 198
AseI ATTAAT 1 cut(s) 1611
Asp700I GAANNNNTTC 1 cut(s) 661
AspLEI GCGC 1 cut(s) 544
AspS9I GGNCC 3 cut(s) 648, 1014, 1210
AsuC2I CCSGG 1 cut(s) 1115
AsuHPI GGTGA 6 cut(s) 169, 994, 1006, 1216, 1407, 1507
AvaI CYCGRG 1 cut(s) 836
BaeGI GKGCMC 1 cut(s) 329
BalI TGGCCA 1 cut(s) 1148
BamHI GGATCC 1 cut(s) 590
BbsI GAAGAC 2 cut(s) 45, 1126
Bbv12I GWGCWC 2 cut(s) 1442, 1460
BbvCI CCTCAGC 1 cut(s) 1689
BbvI GCAGC 6 cut(s) 319, 351, 373, 760, 1295, 1631
BccI CCATC 8 cut(s) 541, 592, 692, 985, 1031, 1533, 1635, 1678
BciT130I CCWGG 3 cut(s) 155, 167, 1218
BclI TGATCA 2 cut(s) 508, 1155
BcnI CCSGG 1 cut(s) 1115
BcoDI GTCTC 1 cut(s) 1184
BfaI CTAG 2 cut(s) 1388, 1469
BfmI CTRYAG 2 cut(s) 57, 1232
BfuAI ACCTGC 1 cut(s) 920
BisI GCNGC 8 cut(s) 27, 333, 359, 362, 365, 774, 1284, 1620
BlsI GCNGC 8 cut(s) 28, 334, 360, 363, 366, 775, 1285, 1621
Bme1390I CCNGG 4 cut(s) 155, 167, 1115, 1218
BmeT110I CYCGRG 1 cut(s) 836
BmgT120I GGNCC 3 cut(s) 648, 1014, 1210
BmiI GGNNCC 3 cut(s) 260, 592, 1681
BmrFI CCNGG 4 cut(s) 155, 167, 1115, 1218
BmsI GCATC 4 cut(s) 22, 783, 896, 1085
BpiI GAAGAC 2 cut(s) 45, 1126
BplI GAGNNNNNCTC 2 cut(s) 246, 278
Bpu10I CCTNAGC 2 cut(s) 1454, 1689
BpuMI CCSGG 1 cut(s) 1115
BsaJI CCNNGG 5 cut(s) 277, 837, 947, 1083, 1652
BsaWI WCCGGW 1 cut(s) 1517
Bsc4I CCNNNNNNNGG 3 cut(s) 877, 1114, 1676
Bse1I ACTGG 4 cut(s) 79, 475, 856, 1167
Bse3DI GCAATG 1 cut(s) 294
BseBI CCWGG 3 cut(s) 155, 167, 1218
BseDI CCNNGG 5 cut(s) 277, 837, 947, 1083, 1652
BseGI GGATG 3 cut(s) 230, 1076, 1544
BseLI CCNNNNNNNGG 3 cut(s) 877, 1114, 1676
BseMI GCAATG 1 cut(s) 294
BseMII CTCAG 4 cut(s) 975, 1077, 1445, 1680
BseNI ACTGG 4 cut(s) 79, 475, 856, 1167
BseRI GAGGAG 3 cut(s) 20, 992, 1522
BseSI GKGCMC 1 cut(s) 329
BseX3I CGGCCG 1 cut(s) 27
BseXI GCAGC 6 cut(s) 319, 351, 373, 760, 1295, 1631
BseYI CCCAGC 1 cut(s) 645
BsgI GTGCAG 1 cut(s) 1359
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 6 cut(s) 29, 650, 1016, 1148, 1212, 1251
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 2 cut(s) 1442, 1460
BsiHKCI CYCGRG 1 cut(s) 836
BsiSI CCGG 2 cut(s) 1115, 1518
BslFI GGGAC 4 cut(s) 187, 481, 718, 726
BslI CCNNNNNNNGG 3 cut(s) 877, 1114, 1676
BsmAI GTCTC 1 cut(s) 1184
BsmBI CGTCTC 1 cut(s) 1184
BsmFI GGGAC 4 cut(s) 187, 481, 718, 726
BsnI GGCC 6 cut(s) 29, 650, 1016, 1148, 1212, 1251
BsoBI CYCGRG 1 cut(s) 836
Bsp1286I GDGCHC 3 cut(s) 329, 1442, 1460
Bsp143I GATC 7 cut(s) 508, 590, 862, 1091, 1110, 1155, 1228
Bsp19I CCATGG 2 cut(s) 947, 1083
BspACI CCGC 3 cut(s) 26, 359, 1303
BspANI GGCC 6 cut(s) 29, 650, 1016, 1148, 1212, 1251
BspCNI CTCAG 4 cut(s) 974, 1076, 1446, 1681
BspLI GGNNCC 3 cut(s) 260, 592, 1681
BspMAI CTGCAG 1 cut(s) 61
BspMI ACCTGC 1 cut(s) 920
BspPI GGATC 4 cut(s) 585, 598, 1086, 1105
BsrDI GCAATG 1 cut(s) 294
BsrI ACTGG 4 cut(s) 79, 475, 856, 1167
BssECI CCNNGG 5 cut(s) 277, 837, 947, 1083, 1652
BssMI GATC 7 cut(s) 508, 590, 862, 1091, 1110, 1155, 1228
BssT1I CCWWGG 4 cut(s) 277, 947, 1083, 1652
Bst2UI CCWGG 3 cut(s) 155, 167, 1218
Bst4CI ACNGT 5 cut(s) 610, 1132, 1178, 1577, 1679
Bst6I CTCTTC 2 cut(s) 779, 1454
BstAPI GCANNNNNTGC 1 cut(s) 305
BstC8I GCNNGC 3 cut(s) 1120, 1146, 1214
BstDEI CTNAG 4 cut(s) 961, 1063, 1454, 1689
BstDSI CCRYGG 2 cut(s) 947, 1083
BstF5I GGATG 3 cut(s) 230, 1076, 1544
BstHHI GCGC 1 cut(s) 544
BstKTI GATC 7 cut(s) 511, 593, 865, 1094, 1113, 1158, 1231
BstMAI GTCTC 1 cut(s) 1184
BstMBI GATC 7 cut(s) 508, 590, 862, 1091, 1110, 1155, 1228
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 7 cut(s) 32, 305, 338, 364, 548, 1283, 1540
BstNI CCWGG 3 cut(s) 155, 167, 1218
BstSCI CCNGG 4 cut(s) 153, 165, 1113, 1216
BstSFI CTRYAG 2 cut(s) 57, 1232
BstSLI GKGCMC 1 cut(s) 329
BstV1I GCAGC 6 cut(s) 319, 351, 373, 760, 1295, 1631
BstV2I GAAGAC 2 cut(s) 45, 1126
BstX2I RGATCY 3 cut(s) 590, 1091, 1110
BstYI RGATCY 3 cut(s) 590, 1091, 1110
BstZI CGGCCG 1 cut(s) 27
BsuRI GGCC 6 cut(s) 29, 650, 1016, 1148, 1212, 1251
BtgI CCRYGG 2 cut(s) 947, 1083
BtsCI GGATG 3 cut(s) 230, 1076, 1544
BtsI GCAGTG 3 cut(s) 153, 327, 1284
BtsIMutI CAGTG 5 cut(s) 153, 327, 1160, 1284, 1317
BveI ACCTGC 1 cut(s) 920
Cac8I GCNNGC 3 cut(s) 1120, 1146, 1214
CaiI CAGNNNCTG 2 cut(s) 645, 935
CfoI GCGC 1 cut(s) 544
Cfr13I GGNCC 3 cut(s) 648, 1014, 1210
CseI GACGC 1 cut(s) 356
CsiI ACCWGGT 2 cut(s) 153, 165
Csp6I GTAC 2 cut(s) 163, 1645
CviAII CATG 2 cut(s) 948, 1084
CviQI GTAC 2 cut(s) 163, 1645
DdeI CTNAG 4 cut(s) 961, 1063, 1454, 1689
DpnI GATC 7 cut(s) 510, 592, 864, 1093, 1112, 1157, 1230
DpnII GATC 7 cut(s) 508, 590, 862, 1091, 1110, 1155, 1228
EaeI YGGCCR 2 cut(s) 27, 1146
EagI CGGCCG 1 cut(s) 27
Eam1104I CTCTTC 2 cut(s) 779, 1454
EarI CTCTTC 2 cut(s) 779, 1454
EclXI CGGCCG 1 cut(s) 27
Eco130I CCWWGG 4 cut(s) 277, 947, 1083, 1652
Eco52I CGGCCG 1 cut(s) 27
Eco57I CTGAAG 1 cut(s) 183
Eco88I CYCGRG 1 cut(s) 836
EcoRI GAATTC 1 cut(s) 432
EcoRII CCWGG 3 cut(s) 153, 165, 1216
EcoT14I CCWWGG 4 cut(s) 277, 947, 1083, 1652
EcoT22I ATGCAT 1 cut(s) 183
ErhI CCWWGG 4 cut(s) 277, 947, 1083, 1652
Esp3I CGTCTC 1 cut(s) 1184
FaeI CATG 2 cut(s) 951, 1087
FalI AAGNNNNNCTT 4 cut(s) 181, 213, 1527, 1559
FaqI GGGAC 4 cut(s) 187, 481, 718, 726
FatI CATG 2 cut(s) 947, 1083
FbaI TGATCA 2 cut(s) 508, 1155
FblI GTMKAC 2 cut(s) 618, 1548
Fnu4HI GCNGC 8 cut(s) 27, 333, 359, 362, 365, 774, 1284, 1620
FokI GGATG 3 cut(s) 217, 1063, 1551
Fsp4HI GCNGC 8 cut(s) 27, 333, 359, 362, 365, 774, 1284, 1620
FspBI CTAG 2 cut(s) 1388, 1469
GlaI GCGC 1 cut(s) 543
GluI GCNGC 8 cut(s) 27, 333, 359, 362, 365, 774, 1284, 1620
GsaI CCCAGC 1 cut(s) 649
HaeIII GGCC 6 cut(s) 29, 650, 1016, 1148, 1212, 1251
HapII CCGG 2 cut(s) 1115, 1518
HgaI GACGC 1 cut(s) 356
HhaI GCGC 1 cut(s) 544
Hin1II CATG 2 cut(s) 951, 1087
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HinfI GANTC 4 cut(s) 269, 908, 1264, 1446
HpaII CCGG 2 cut(s) 1115, 1518
HphI GGTGA 6 cut(s) 169, 994, 1006, 1216, 1407, 1507
Hpy166II GTNNAC 7 cut(s) 40, 151, 371, 619, 637, 1515, 1549
Hpy188I TCNGA 4 cut(s) 942, 1008, 1100, 1675
Hpy188III TCNNGA 5 cut(s) 495, 836, 846, 1108, 1453
Hpy8I GTNNAC 7 cut(s) 40, 151, 371, 619, 637, 1515, 1549
Hpy99I CGWCG 2 cut(s) 812, 1100
HpyAV CCTTC 5 cut(s) 244, 283, 1069, 1201, 1560
HpyCH4III ACNGT 5 cut(s) 610, 1132, 1178, 1577, 1679
HpyCH4IV ACGT 2 cut(s) 161, 810
HpyF10VI GCNNNNNNNGC 7 cut(s) 32, 305, 338, 364, 548, 1283, 1540
HpyF3I CTNAG 4 cut(s) 961, 1063, 1454, 1689
HpySE526I ACGT 2 cut(s) 161, 810
Hsp92II CATG 2 cut(s) 951, 1087
HspAI GCGC 1 cut(s) 542
Ksp22I TGATCA 2 cut(s) 508, 1155
Kzo9I GATC 7 cut(s) 508, 590, 862, 1091, 1110, 1155, 1228
LmnI GCTCC 1 cut(s) 426
Lsp1109I GCAGC 6 cut(s) 319, 351, 373, 760, 1295, 1631
LweI GCATC 4 cut(s) 22, 783, 896, 1085
MabI ACCWGGT 2 cut(s) 153, 165
MaeI CTAG 2 cut(s) 1388, 1469
MaeII ACGT 2 cut(s) 161, 810
MaeIII GTNAC 6 cut(s) 85, 157, 211, 604, 854, 964
MalI GATC 7 cut(s) 510, 592, 864, 1093, 1112, 1157, 1230
MboI GATC 7 cut(s) 508, 590, 862, 1091, 1110, 1155, 1228
MfeI CAATTG 3 cut(s) 400, 1245, 1500
MflI RGATCY 3 cut(s) 590, 1091, 1110
MhlI GDGCHC 3 cut(s) 329, 1442, 1460
MlsI TGGCCA 1 cut(s) 1148
MluNI TGGCCA 1 cut(s) 1148
MlyI GAGTC 2 cut(s) 1258, 1440
MmeI TCCRAC 1 cut(s) 1078
Mox20I TGGCCA 1 cut(s) 1148
Mph1103I ATGCAT 1 cut(s) 183
MroXI GAANNNNTTC 1 cut(s) 661
MscI TGGCCA 1 cut(s) 1148
MseI TTAA 5 cut(s) 1170, 1370, 1601, 1611, 1702
MslI CAYNNNNRTG 1 cut(s) 671
Msp20I TGGCCA 1 cut(s) 1148
MspA1I CMGCKG 1 cut(s) 364
MspI CCGG 2 cut(s) 1115, 1518
MspR9I CCNGG 4 cut(s) 155, 167, 1115, 1218
MunI CAATTG 3 cut(s) 400, 1245, 1500
MvaI CCWGG 3 cut(s) 155, 167, 1218
MwoI GCNNNNNNNGC 7 cut(s) 32, 305, 338, 364, 548, 1283, 1540
NciI CCSGG 1 cut(s) 1115
NcoI CCATGG 2 cut(s) 947, 1083
NdeII GATC 7 cut(s) 508, 590, 862, 1091, 1110, 1155, 1228
NlaIII CATG 2 cut(s) 951, 1087
NlaIV GGNNCC 3 cut(s) 260, 592, 1681
NmuCI GTSAC 4 cut(s) 157, 211, 604, 854
NsiI ATGCAT 1 cut(s) 183
PdmI GAANNNNTTC 1 cut(s) 661
PfeI GAWTC 2 cut(s) 269, 908
PkrI GCNGC 8 cut(s) 28, 334, 360, 363, 366, 775, 1285, 1621
PleI GAGTC 2 cut(s) 1258, 1440
PpsI GAGTC 2 cut(s) 1258, 1440
PshBI ATTAAT 1 cut(s) 1611
PsiI TTATAA 1 cut(s) 1406
Psp6I CCWGG 3 cut(s) 153, 165, 1216
PspFI CCCAGC 1 cut(s) 645
PspGI CCWGG 3 cut(s) 153, 165, 1216
PspN4I GGNNCC 3 cut(s) 260, 592, 1681
PspPI GGNCC 3 cut(s) 648, 1014, 1210
PstI CTGCAG 1 cut(s) 61
PstNI CAGNNNCTG 2 cut(s) 645, 935
PsuI RGATCY 3 cut(s) 590, 1091, 1110
PvuII CAGCTG 1 cut(s) 364
RsaI GTAC 2 cut(s) 164, 1646
RsaNI GTAC 2 cut(s) 163, 1645
RseI CAYNNNNRTG 1 cut(s) 671
SaqAI TTAA 5 cut(s) 1170, 1370, 1601, 1611, 1702
SatI GCNGC 8 cut(s) 27, 333, 359, 362, 365, 774, 1284, 1620
Sau3AI GATC 7 cut(s) 508, 590, 862, 1091, 1110, 1155, 1228
Sau96I GGNCC 3 cut(s) 648, 1014, 1210
SchI GAGTC 2 cut(s) 1258, 1440
ScrFI CCNGG 4 cut(s) 155, 167, 1115, 1218
SduI GDGCHC 3 cut(s) 329, 1442, 1460
SexAI ACCWGGT 2 cut(s) 153, 165
SfaNI GCATC 4 cut(s) 22, 783, 896, 1085
SfcI CTRYAG 2 cut(s) 57, 1232
SmiMI CAYNNNNRTG 1 cut(s) 671
SsiI CCGC 3 cut(s) 26, 359, 1303
SspI AATATT 1 cut(s) 208
SspMI CTAG 2 cut(s) 1388, 1469
StyD4I CCNGG 4 cut(s) 153, 165, 1113, 1216
StyI CCWWGG 4 cut(s) 277, 947, 1083, 1652
TaaI ACNGT 5 cut(s) 610, 1132, 1178, 1577, 1679
TaiI ACGT 2 cut(s) 164, 813
TaqI TCGA 3 cut(s) 807, 1262, 1396
TauI GCSGC 2 cut(s) 29, 361
TfiI GAWTC 2 cut(s) 269, 908
Tru1I TTAA 5 cut(s) 1170, 1370, 1601, 1611, 1702
Tru9I TTAA 5 cut(s) 1170, 1370, 1601, 1611, 1702
TscAI CASTG 5 cut(s) 153, 334, 1167, 1284, 1317
TseFI GTSAC 4 cut(s) 157, 211, 604, 854
TseI GCWGC 6 cut(s) 332, 361, 364, 773, 1283, 1619
Tsp45I GTSAC 4 cut(s) 157, 211, 604, 854
TspDTI ATGAA 4 cut(s) 534, 568, 936, 1442
TspGWI ACGGA 3 cut(s) 860, 1059, 1084
TspRI CASTG 5 cut(s) 153, 334, 1167, 1284, 1317
VspI ATTAAT 1 cut(s) 1611
XapI RAATTY 2 cut(s) 432, 753
XcmI CCANNNNNNNNNTGG 1 cut(s) 1115
XmiI GTMKAC 2 cut(s) 618, 1548
XmnI GAANNNNTTC 1 cut(s) 661
XspI CTAG 2 cut(s) 1388, 1469
Zsp2I ATGCAT 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.