Rw2G003370

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004338
Physical Location & Seq
Forward (+)
3172103 .. 3174872
2770 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G003370.1

Sequence Viewer

Length: 1740 bp
ATGGAGGAGAAAATGAGTGGGTATGCGGCCGATGCTCTGGACTACAAAGGGTTTCCTGCTGATAAATCCAGAACTGGTGGTTGGGTTGCCGCGGCACTTATACTAGGAATTGAAATATGTGAAAGGCTATCAACTATGGGAATAGCAGTGAACCTGGTGACATACCTAGTTGGGACAATGCATTTGCCAAGCTCAACTTCAGCAAATATTGTCACAGACTTTGCAGGCACATCCTTCCTCCTTTGTTTGCTAGGAGGCTTCCTCGCCGATTCCTTCCTTGGCAGATACAAGACCATTGCAATCTTTGCTTCAATACAGACATTGGGCACAATTGCATTAGCAATATCAGTAAAATTGCCACAGCTGCGGCCACCTCATTGTGACATCACTGCTGCCAACAACAACTGCAAACAAGCCAATGACTTCCAAACGGGATTCCTTTACTTGGCTTTATATATAATTGCATTAGCGATTGGTGGGCTGAAATCAAGTGTTTCTGGATTTGGAACTGATCAATTTGATGAAAAAGATGACAAAGAGAAAGCGCAGATGGCATATTTCTTCAATAGATTTTTCTTTTTCATTAGCACTGGAACCTTGATGGCAGTCACAGTCCTTGTCTACATACAAGACAAAGTGGACAGAAGCTGGGCCTATGGAATATGTTCCATTTCAATGTTCATAGCCATCTTGGTGTTTTTTTCGGGGACTAAAAGATACAGATATAAGAAAAGCTTGGGAAGTCCCATAGTTCAAATTTTTCAGGTTATTGTGGCTGCTGTAAGGAAGAGAAAGATGCCACTTCCATTCGACGTTGCCTTGTTGTTTGAGAATTATCCCGAGGCTTCAAGAATTGGTCACACTGATCAGTTCCGTTTCTTGGACAAGGCAGCTATTGTGGCAGAAGAAGATTTTGTGAAAAATGCACCTGCAGGTTCTGTTCCGAACCCATGGAATTTATGCTCAGTTACAAGAGTGGAGGAGGTGAAAATGATGGTGAAACTACTTCCGATATGGGCCACAACCATCATGTTTTGGACAGCATACGCACAGATGCTCACCTTCTCAGTAGAACAAGCATCCACCATGGAGAGATCCATTGGAAAGTTTCAAATCCCGGCTGGCTCTCTCACAGTCTTCTTTGTGTTAGCCATATTGATCACATTGGCTGTTAATGACCGTCTCATTATGCCAATTTGGAAGAAATGGAAGGGCCAAGCAGGTTTCACCAATCTACAGAGAATTGCAATTGGCCTTGTCCTATCGACTCTTGGAATGGCAGCGGCAGCACTAACCGAGAGGAGGCGGTTATCAGTGGCTAAAGAAATGAGAACTATGAGTACAGGTCCTCTGCCTATTAGTGTGTACTTGTTAATCCCACAATTCTTCTTGGTGGGTTCTGGTGAAGCTTTCATCTACACTGGCCAGCTCGATTTCTTTATAACTCAATCACCAAAAGGAATGAAAACTATGAGCACAGGACTCTTCCTGAGCACTTTATCACTAGGCTTCTTTGTCAGTAGCTTCTTGGTTTCAATTGTGAGGAGGTTCACCGGAAGCAAAGATGGGCAAGGATGGCTTGCAGACAATATCAACTACGGAAGGCTTGACTGTTTCTATGGACTTTTGAGCGTTTTAAGTGTCATAAATTTTGTAGCATATCTTGTTTGTGCAAGATGGTACAAGCCAAGAAAACCTACCAAACCATCCAACGGTTCCTCAGCTGAGGATAGGTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0006950 GO:0008150 GO:0009611 GO:0009719 GO:0009725 GO:0009753 GO:0010033 GO:0042221 GO:0050896 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

579

Amino Acids

63.99

Weight (kDa)

9.33

Isoelectric Point (pI)

34.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 96 - 526 1.7e-100 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1442
AarI CACCTGC 1 cut(s) 937
Acc36I ACCTGC 3 cut(s) 923, 937, 1211
AccI GTMKAC 1 cut(s) 621
AccII CGCG 1 cut(s) 92
AciI CCGC 6 cut(s) 26, 90, 92, 367, 1283, 1306
AclWI GGATC 1 cut(s) 1089
AcoI YGGCCR 3 cut(s) 27, 368, 1423
AcsI RAATTY 3 cut(s) 756, 955, 1648
AcuI CTGAAG 1 cut(s) 183
AfaI GTAC 3 cut(s) 1342, 1367, 1682
AfiI CCNNNNNNNGG 4 cut(s) 445, 880, 1302, 1712
AgsI TTSAA 8 cut(s) 113, 312, 565, 675, 755, 849, 1112, 1536
AjnI CCWGG 1 cut(s) 153
AjuI GAANNNNNNNTTGG 4 cut(s) 64, 96, 181, 213
AluBI AGCT 9 cut(s) 192, 364, 648, 735, 893, 1409, 1429, 1524, 1724
AluI AGCT 9 cut(s) 192, 364, 648, 735, 893, 1409, 1429, 1524, 1724
Alw21I GWGCWC 2 cut(s) 1478, 1496
Alw26I GTCTC 1 cut(s) 1187
AlwI GGATC 1 cut(s) 1089
AlwNI CAGNNNCTG 2 cut(s) 648, 938
Ama87I CYCGRG 1 cut(s) 839
AoxI GGCC 7 cut(s) 27, 368, 651, 1017, 1213, 1252, 1423
ApeKI GCWGC 6 cut(s) 364, 392, 776, 890, 1280, 1286
ApoI RAATTY 3 cut(s) 756, 955, 1648
ArsI GACNNNNNNTTYG 2 cut(s) 166, 198
Asp700I GAANNNNTTC 1 cut(s) 664
AspLEI GCGC 1 cut(s) 547
AspS9I GGNCC 4 cut(s) 651, 1017, 1213, 1346
AsuC2I CCSGG 1 cut(s) 1118
AsuHPI GGTGA 8 cut(s) 169, 997, 1009, 1051, 1219, 1415, 1443, 1543
AvaI CYCGRG 1 cut(s) 839
AvaII GGWCC 1 cut(s) 1346
BaeGI GKGCMC 1 cut(s) 329
BalI TGGCCA 1 cut(s) 1425
BbsI GAAGAC 1 cut(s) 1129
Bbv12I GWGCWC 2 cut(s) 1478, 1496
BbvCI CCTCAGC 2 cut(s) 1720, 1725
BbvI GCAGC 6 cut(s) 351, 379, 763, 902, 1292, 1298
BccI CCATC 9 cut(s) 544, 595, 695, 988, 1034, 1559, 1569, 1671, 1714
BciT130I CCWGG 1 cut(s) 155
BclI TGATCA 3 cut(s) 511, 865, 1158
BcnI CCSGG 1 cut(s) 1118
BcoDI GTCTC 1 cut(s) 1187
BfaI CTAG 4 cut(s) 104, 167, 251, 1505
BfmI CTRYAG 2 cut(s) 930, 1235
BfuAI ACCTGC 3 cut(s) 923, 937, 1211
Bme1390I CCNGG 2 cut(s) 155, 1118
Bme18I GGWCC 1 cut(s) 1346
BmeT110I CYCGRG 1 cut(s) 839
BmgT120I GGNCC 4 cut(s) 651, 1017, 1213, 1346
BmiI GGNNCC 2 cut(s) 595, 1717
BmrFI CCNGG 2 cut(s) 155, 1118
BmsI GCATC 4 cut(s) 22, 786, 1044, 1088
BpiI GAAGAC 1 cut(s) 1129
BplI GAGNNNNNCTC 2 cut(s) 246, 278
Bpu10I CCTNAGC 3 cut(s) 1490, 1720, 1725
BpuMI CCSGG 1 cut(s) 1118
BsaJI CCNNGG 5 cut(s) 90, 277, 840, 950, 1086
BsaWI WCCGGW 1 cut(s) 1553
Bsc4I CCNNNNNNNGG 4 cut(s) 445, 880, 1302, 1712
Bse1I ACTGG 3 cut(s) 79, 595, 1426
Bse3DI GCAATG 1 cut(s) 294
BseBI CCWGG 1 cut(s) 155
BseDI CCNNGG 5 cut(s) 90, 277, 840, 950, 1086
BseGI GGATG 4 cut(s) 230, 1079, 1580, 1706
BseLI CCNNNNNNNGG 4 cut(s) 445, 880, 1302, 1712
BseMI GCAATG 1 cut(s) 294
BseMII CTCAG 5 cut(s) 978, 1080, 1481, 1716, 1734
BseNI ACTGG 3 cut(s) 79, 595, 1426
BseRI GAGGAG 4 cut(s) 20, 995, 1315, 1558
BseSI GKGCMC 1 cut(s) 329
BseX3I CGGCCG 1 cut(s) 27
BseXI GCAGC 6 cut(s) 351, 379, 763, 902, 1292, 1298
BseYI CCCAGC 1 cut(s) 648
Bsh1236I CGCG 1 cut(s) 92
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 7 cut(s) 29, 370, 653, 1019, 1215, 1254, 1425
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 2 cut(s) 1478, 1496
BsiHKCI CYCGRG 1 cut(s) 839
BsiSI CCGG 2 cut(s) 1118, 1554
BslFI GGGAC 3 cut(s) 187, 721, 729
BslI CCNNNNNNNGG 4 cut(s) 445, 880, 1302, 1712
BsmAI GTCTC 1 cut(s) 1187
BsmBI CGTCTC 1 cut(s) 1187
BsmFI GGGAC 3 cut(s) 187, 721, 729
BsnI GGCC 7 cut(s) 29, 370, 653, 1019, 1215, 1254, 1425
BsoBI CYCGRG 1 cut(s) 839
Bsp1286I GDGCHC 3 cut(s) 329, 1478, 1496
Bsp143I GATC 4 cut(s) 511, 865, 1094, 1158
Bsp19I CCATGG 2 cut(s) 950, 1086
BspACI CCGC 6 cut(s) 26, 90, 92, 367, 1283, 1306
BspANI GGCC 7 cut(s) 29, 370, 653, 1019, 1215, 1254, 1425
BspCNI CTCAG 5 cut(s) 977, 1079, 1482, 1717, 1733
BspFNI CGCG 1 cut(s) 92
BspLI GGNNCC 2 cut(s) 595, 1717
BspMAI CTGCAG 1 cut(s) 934
BspMI ACCTGC 3 cut(s) 923, 937, 1211
BspPI GGATC 1 cut(s) 1089
BsrDI GCAATG 1 cut(s) 294
BsrI ACTGG 3 cut(s) 79, 595, 1426
BssECI CCNNGG 5 cut(s) 90, 277, 840, 950, 1086
BssMI GATC 4 cut(s) 511, 865, 1094, 1158
BssT1I CCWWGG 3 cut(s) 277, 950, 1086
Bst2UI CCWGG 1 cut(s) 155
Bst4CI ACNGT 5 cut(s) 613, 1135, 1181, 1613, 1715
Bst6I CTCTTC 2 cut(s) 782, 1490
BstAPI GCANNNNNTGC 1 cut(s) 305
BstC8I GCNNGC 4 cut(s) 226, 1123, 1427, 1581
BstDEI CTNAG 5 cut(s) 964, 1066, 1490, 1720, 1725
BstDSI CCRYGG 3 cut(s) 90, 950, 1086
BstF5I GGATG 4 cut(s) 230, 1079, 1580, 1706
BstFNI CGCG 1 cut(s) 92
BstHHI GCGC 1 cut(s) 547
BstKTI GATC 4 cut(s) 514, 868, 1097, 1161
BstMAI GTCTC 1 cut(s) 1187
BstMBI GATC 4 cut(s) 511, 865, 1094, 1158
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 7 cut(s) 32, 305, 364, 551, 899, 1286, 1576
BstNI CCWGG 1 cut(s) 155
BstSCI CCNGG 2 cut(s) 153, 1116
BstSFI CTRYAG 2 cut(s) 930, 1235
BstSLI GKGCMC 1 cut(s) 329
BstUI CGCG 1 cut(s) 92
BstV1I GCAGC 6 cut(s) 351, 379, 763, 902, 1292, 1298
BstV2I GAAGAC 1 cut(s) 1129
BstX2I RGATCY 1 cut(s) 1094
BstYI RGATCY 1 cut(s) 1094
BstZI CGGCCG 1 cut(s) 27
BsuRI GGCC 7 cut(s) 29, 370, 653, 1019, 1215, 1254, 1425
BtgI CCRYGG 3 cut(s) 90, 950, 1086
BtsCI GGATG 4 cut(s) 230, 1079, 1580, 1706
BtsI GCAGTG 2 cut(s) 153, 387
BtsIMutI CAGTG 6 cut(s) 153, 387, 588, 861, 1320, 1419
BveI ACCTGC 3 cut(s) 923, 937, 1211
Cac8I GCNNGC 4 cut(s) 226, 1123, 1427, 1581
CaiI CAGNNNCTG 2 cut(s) 648, 938
CfoI GCGC 1 cut(s) 547
Cfr13I GGNCC 4 cut(s) 651, 1017, 1213, 1346
Cfr42I CCGCGG 1 cut(s) 93
CsiI ACCWGGT 1 cut(s) 153
Csp6I GTAC 3 cut(s) 1341, 1366, 1681
CviAII CATG 3 cut(s) 951, 1030, 1087
CviQI GTAC 3 cut(s) 1341, 1366, 1681
DdeI CTNAG 5 cut(s) 964, 1066, 1490, 1720, 1725
DpnI GATC 4 cut(s) 513, 867, 1096, 1160
DpnII GATC 4 cut(s) 511, 865, 1094, 1158
EaeI YGGCCR 3 cut(s) 27, 368, 1423
EagI CGGCCG 1 cut(s) 27
Eam1104I CTCTTC 2 cut(s) 782, 1490
EarI CTCTTC 2 cut(s) 782, 1490
EclXI CGGCCG 1 cut(s) 27
Eco130I CCWWGG 3 cut(s) 277, 950, 1086
Eco47I GGWCC 1 cut(s) 1346
Eco52I CGGCCG 1 cut(s) 27
Eco57I CTGAAG 1 cut(s) 183
Eco88I CYCGRG 1 cut(s) 839
EcoO109I RGGNCCY 1 cut(s) 1346
EcoRII CCWGG 1 cut(s) 153
EcoT14I CCWWGG 3 cut(s) 277, 950, 1086
EcoT22I ATGCAT 1 cut(s) 183
ErhI CCWWGG 3 cut(s) 277, 950, 1086
Esp3I CGTCTC 1 cut(s) 1187
FaeI CATG 3 cut(s) 954, 1033, 1090
FalI AAGNNNNNCTT 6 cut(s) 181, 213, 719, 751, 1563, 1595
FaqI GGGAC 3 cut(s) 187, 721, 729
FatI CATG 3 cut(s) 950, 1029, 1086
FbaI TGATCA 3 cut(s) 511, 865, 1158
FblI GTMKAC 1 cut(s) 621
FokI GGATG 4 cut(s) 217, 1066, 1587, 1693
FspBI CTAG 4 cut(s) 104, 167, 251, 1505
GlaI GCGC 1 cut(s) 546
GsaI CCCAGC 1 cut(s) 652
HaeIII GGCC 7 cut(s) 29, 370, 653, 1019, 1215, 1254, 1425
HapII CCGG 2 cut(s) 1118, 1554
HhaI GCGC 1 cut(s) 547
Hin1II CATG 3 cut(s) 954, 1033, 1090
Hin6I GCGC 1 cut(s) 545
HinP1I GCGC 1 cut(s) 545
HindIII AAGCTT 2 cut(s) 733, 1407
HinfI GANTC 4 cut(s) 269, 435, 1267, 1482
HpaII CCGG 2 cut(s) 1118, 1554
HphI GGTGA 8 cut(s) 169, 997, 1009, 1051, 1219, 1415, 1443, 1543
Hpy166II GTNNAC 5 cut(s) 151, 622, 640, 1366, 1551
Hpy188I TCNGA 2 cut(s) 945, 1011
Hpy188III TCNNGA 6 cut(s) 38, 69, 498, 839, 849, 1489
Hpy8I GTNNAC 5 cut(s) 151, 622, 640, 1366, 1551
Hpy99I CGWCG 1 cut(s) 815
HpyAV CCTTC 5 cut(s) 244, 283, 1072, 1204, 1596
HpyCH4III ACNGT 5 cut(s) 613, 1135, 1181, 1613, 1715
HpyCH4IV ACGT 1 cut(s) 813
HpyF10VI GCNNNNNNNGC 7 cut(s) 32, 305, 364, 551, 899, 1286, 1576
HpyF3I CTNAG 5 cut(s) 964, 1066, 1490, 1720, 1725
HpySE526I ACGT 1 cut(s) 813
Hsp92II CATG 3 cut(s) 954, 1033, 1090
HspAI GCGC 1 cut(s) 545
Ksp22I TGATCA 3 cut(s) 511, 865, 1158
KspI CCGCGG 1 cut(s) 93
Kzo9I GATC 4 cut(s) 511, 865, 1094, 1158
Lsp1109I GCAGC 6 cut(s) 351, 379, 763, 902, 1292, 1298
LweI GCATC 4 cut(s) 22, 786, 1044, 1088
MabI ACCWGGT 1 cut(s) 153
MaeI CTAG 4 cut(s) 104, 167, 251, 1505
MaeII ACGT 1 cut(s) 813
MaeIII GTNAC 6 cut(s) 157, 211, 380, 607, 857, 967
MalI GATC 4 cut(s) 513, 867, 1096, 1160
MboI GATC 4 cut(s) 511, 865, 1094, 1158
MboII GAAGA 8 cut(s) 553, 799, 917, 920, 1129, 1213, 1378, 1477
MfeI CAATTG 3 cut(s) 330, 1248, 1536
MflI RGATCY 1 cut(s) 1094
MhlI GDGCHC 3 cut(s) 329, 1478, 1496
MlsI TGGCCA 1 cut(s) 1425
MluNI TGGCCA 1 cut(s) 1425
MlyI GAGTC 2 cut(s) 1261, 1476
MmeI TCCRAC 1 cut(s) 1734
Mox20I TGGCCA 1 cut(s) 1425
Mph1103I ATGCAT 1 cut(s) 183
MroXI GAANNNNTTC 1 cut(s) 664
MscI TGGCCA 1 cut(s) 1425
MseI TTAA 3 cut(s) 1173, 1373, 1637
MslI CAYNNNNRTG 2 cut(s) 674, 692
Msp20I TGGCCA 1 cut(s) 1425
MspA1I CMGCKG 4 cut(s) 92, 364, 1283, 1724
MspI CCGG 2 cut(s) 1118, 1554
MspR9I CCNGG 2 cut(s) 155, 1118
MunI CAATTG 3 cut(s) 330, 1248, 1536
MvaI CCWGG 1 cut(s) 155
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 7 cut(s) 32, 305, 364, 551, 899, 1286, 1576
NciI CCSGG 1 cut(s) 1118
NcoI CCATGG 2 cut(s) 950, 1086
NdeII GATC 4 cut(s) 511, 865, 1094, 1158
NlaIII CATG 3 cut(s) 954, 1033, 1090
NlaIV GGNNCC 2 cut(s) 595, 1717
NmuCI GTSAC 5 cut(s) 157, 211, 380, 607, 857
NsiI ATGCAT 1 cut(s) 183
PaqCI CACCTGC 1 cut(s) 937
PdmI GAANNNNTTC 1 cut(s) 664
PfeI GAWTC 2 cut(s) 269, 435
PleI GAGTC 2 cut(s) 1261, 1476
PpsI GAGTC 2 cut(s) 1261, 1476
PpuMI RGGWCCY 1 cut(s) 1346
PsiI TTATAA 1 cut(s) 1442
Psp5II RGGWCCY 1 cut(s) 1346
Psp6I CCWGG 1 cut(s) 153
PspFI CCCAGC 1 cut(s) 648
PspGI CCWGG 1 cut(s) 153
PspN4I GGNNCC 2 cut(s) 595, 1717
PspPI GGNCC 4 cut(s) 651, 1017, 1213, 1346
PspPPI RGGWCCY 1 cut(s) 1346
PsrI GAACNNNNNNTAC 2 cut(s) 1324, 1356
PstI CTGCAG 1 cut(s) 934
PstNI CAGNNNCTG 2 cut(s) 648, 938
PsuI RGATCY 1 cut(s) 1094
PvuII CAGCTG 2 cut(s) 364, 1724
RsaI GTAC 3 cut(s) 1342, 1367, 1682
RsaNI GTAC 3 cut(s) 1341, 1366, 1681
RseI CAYNNNNRTG 2 cut(s) 674, 692
SacII CCGCGG 1 cut(s) 93
SaqAI TTAA 3 cut(s) 1173, 1373, 1637
Sau3AI GATC 4 cut(s) 511, 865, 1094, 1158
Sau96I GGNCC 4 cut(s) 651, 1017, 1213, 1346
SbfI CCTGCAGG 1 cut(s) 934
SchI GAGTC 2 cut(s) 1261, 1476
ScrFI CCNGG 2 cut(s) 155, 1118
SdaI CCTGCAGG 1 cut(s) 934
SduI GDGCHC 3 cut(s) 329, 1478, 1496
SexAI ACCWGGT 1 cut(s) 153
SfaNI GCATC 4 cut(s) 22, 786, 1044, 1088
SfcI CTRYAG 2 cut(s) 930, 1235
Sfr303I CCGCGG 1 cut(s) 93
SgrBI CCGCGG 1 cut(s) 93
SinI GGWCC 1 cut(s) 1346
SmiMI CAYNNNNRTG 2 cut(s) 674, 692
Sse8387I CCTGCAGG 1 cut(s) 934
SsiI CCGC 6 cut(s) 26, 90, 92, 367, 1283, 1306
SspI AATATT 1 cut(s) 208
SspMI CTAG 4 cut(s) 104, 167, 251, 1505
StyD4I CCNGG 2 cut(s) 153, 1116
StyI CCWWGG 3 cut(s) 277, 950, 1086
TaaI ACNGT 5 cut(s) 613, 1135, 1181, 1613, 1715
TaiI ACGT 1 cut(s) 816
TaqI TCGA 3 cut(s) 810, 1265, 1431
TatI WGTACW 2 cut(s) 1340, 1365
TauI GCSGC 5 cut(s) 29, 92, 95, 370, 1286
TfiI GAWTC 2 cut(s) 269, 435
Tru1I TTAA 3 cut(s) 1173, 1373, 1637
Tru9I TTAA 3 cut(s) 1173, 1373, 1637
TscAI CASTG 6 cut(s) 153, 394, 595, 868, 1320, 1426
TseFI GTSAC 5 cut(s) 157, 211, 380, 607, 857
TseI GCWGC 6 cut(s) 364, 392, 776, 890, 1280, 1286
Tsp45I GTSAC 5 cut(s) 157, 211, 380, 607, 857
TspDTI ATGAA 5 cut(s) 537, 571, 670, 1402, 1478
TspGWI ACGGA 2 cut(s) 863, 1614
TspRI CASTG 6 cut(s) 153, 394, 595, 868, 1320, 1426
VpaK11BI GGWCC 1 cut(s) 1346
XapI RAATTY 3 cut(s) 756, 955, 1648
XmiI GTMKAC 1 cut(s) 621
XmnI GAANNNNTTC 1 cut(s) 664
XspI CTAG 4 cut(s) 104, 167, 251, 1505
Zsp2I ATGCAT 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.