FvH4_1g04020

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0003748
Physical Location & Seq
Reverse (-)
2132711 .. 2135722
3012 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g04020.t1

Sequence Viewer

Length: 1548 bp
ATGGCGGAACCAAAACCAGATACACCATTGATCCCACCATCATCTAGACCCTCAAACAATAGGAACCTCCCCGACTTCAAAAAATCCATTAAGCTAAAATATGTGAAGCTTGGTTACCATATTCTCATCACCCATGGAATGTACCTTTTCCTTTCCCCTCTTGTCATTGTTATTGCTGCCCAGCTCTCCACATTTTCGCTCCAAGATCTATATGATCTATGGGAGCATCTCCAATACAACCTGATTTCGGTGATCATCTGCTCGACCCTCCTGGTGTTCCTATCCACTGTATACTTTCTTACCCGCCCTCGCCCTGTGTACCTTGTTAACTTTTCTTGTTACAAGCCAGAAGAAACTCGAAAATGCACGAAAAAGATTTTCATGGATCAGTCTACGATGACTGGTACATTCACAGAAGAGAATCTACAGTTCCAGAGAAGGATCCTTGAGAGATCCGGCCTTGGTGATTCAACATACCTTCCAGAGGCTGTACTTAACATTCCCCCAAATCCGTCAATGAAAGAAGCCAGGAAAGAAGCTGAGGCAGTGATGTATGGTGCCATTGACGAGCTATTTGCCAAGACATCTGTAAAACCCAAGGACATCGGAATCTTGATTGTCAACTGCAGCTTGTTCAATCCAACTCCATCTCTTTCTGCCATGGTTATCAATCATTACCAGCTTCGAGGGAATATAGTCAGCTACAATCTTGGTGGAATGGGATGTAGCGCGGGGCTCATCTCAATTGATCTTGCTCAAAAGCTTCTTCAGGTGCATCCTAACTCTTATGCGCTGGTTATCAGCATGGAGAACATTACATTGAACTGGTACTTTGGCAATGATCGTTCAAAGCTTGTTTCAAATTGCTTATTCCGGATGGGAGGGGCTGCTATACTTCTTTCAAACAAAAACTCAGACAGAAGGAGGTCCAAGTACCAATTAGTCCATACTGTCCGCACGCACAAGGGTGCTGATGACAAGTGCTTTAGCTGTGTTACTCAAGAAGAAGATGATGCTGGGCATATTGGTGTCACACTATCGAAGGACCTGATGGCTGTAGCAGGTGATGCTTTGAAGACCAATATCACCACACTGGGGCCTCTTGTTCTTCCAATGTCCGAGCAGCTGCTTTTCTTTGCTACATTGGTGGGTAGGAAACTGTTCAAGATGAAGATCAAACCTTACATCCCAGATTTCAGACTTGCTTTTGACCATTTCTGCATTCATGCTGGGGGAAGAGCCGTCTTGGATGAATTGGAGAAGAACCTGCATCTGTCTGATTGGCATATGGAGCCATCAAGGATGACACTATACCGATTTGGAAACACCTCAAGCAGTTCTCTCTGGTATGAGTTAGCTTACACAGAAGCAAAGGGAAGGATTAAGAAAGGAGATAGAACATGGCAGATAGCTTTCGGGTCGGGATTCAAGTGTAACAGTGCTGTGTGGAAAGCTCTCAAGACTATAGATCCTGCCAAGGAAAAGAGCCCGTGGATGGCTGAAATTGATCAGTTCCCAGTTGATGTTCCAAGAGTGACAACCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

516

Amino Acids

58.13

Weight (kDa)

9.06

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 98 - 387 3e-144 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 404 - 484 2.6e-10 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 406 - 482 1.5e-08 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1052
Acc36I ACCTGC 2 cut(s) 1052, 1275
AccB1I GGYRCC 1 cut(s) 557
AccI GTMKAC 2 cut(s) 291, 392
AccII CGCG 1 cut(s) 731
AccIII TCCGGA 1 cut(s) 873
AciI CCGC 4 cut(s) 5, 304, 731, 955
AclWI GGATC 6 cut(s) 25, 393, 436, 447, 449, 1463
AcuI CTGAAG 1 cut(s) 752
AfaI GTAC 6 cut(s) 143, 320, 406, 492, 830, 935
AfiI CCNNNNNNNGG 4 cut(s) 247, 484, 1095, 1495
AjnI CCWGG 2 cut(s) 270, 527
AleI CACNNNNGTG 1 cut(s) 966
AlwI GGATC 6 cut(s) 25, 393, 436, 447, 449, 1463
AlwNI CAGNNNCTG 1 cut(s) 488
Aor13HI TCCGGA 1 cut(s) 873
AoxI GGCC 2 cut(s) 457, 1097
ApeKI GCWGC 5 cut(s) 176, 627, 887, 1123, 1126
ArsI GACNNNNNNTTYG 2 cut(s) 557, 589
Asp700I GAANNNNTTC 1 cut(s) 1160
AspLEI GCGC 2 cut(s) 731, 793
AspS9I GGNCC 3 cut(s) 927, 1045, 1097
AsuHPI GGTGA 5 cut(s) 121, 262, 476, 1076, 1078
AvaII GGWCC 2 cut(s) 927, 1045
BamHI GGATCC 1 cut(s) 441
BanI GGYRCC 1 cut(s) 557
BanII GRGCYC 2 cut(s) 738, 1490
BarI GAAGNNNNNNTAC 4 cut(s) 98, 130, 408, 440
BbsI GAAGAC 1 cut(s) 1082
BbvCI CCTCAGC 1 cut(s) 540
BbvI GCAGC 5 cut(s) 163, 639, 874, 1113, 1135
BccI CCATC 6 cut(s) 46, 655, 871, 1045, 1303, 1489
BceAI ACGGC 1 cut(s) 1226
BcgI CGANNNNNNTGC 2 cut(s) 557, 591
BciT130I CCWGG 2 cut(s) 272, 529
BclI TGATCA 2 cut(s) 252, 1507
BfaI CTAG 1 cut(s) 45
BfmI CTRYAG 4 cut(s) 425, 625, 1056, 1464
BfuAI ACCTGC 2 cut(s) 1052, 1275
BglII AGATCT 1 cut(s) 205
BisI GCNGC 5 cut(s) 177, 628, 888, 1124, 1127
BlsI GCNGC 5 cut(s) 178, 629, 889, 1125, 1128
Bme1390I CCNGG 2 cut(s) 272, 529
Bme18I GGWCC 2 cut(s) 927, 1045
BmgT120I GGNCC 3 cut(s) 927, 1045, 1097
BmiI GGNNCC 6 cut(s) 9, 65, 443, 559, 1098, 1293
BmrFI CCNGG 2 cut(s) 272, 529
BmrI ACTGGG 2 cut(s) 1103, 1511
BmsI GCATC 5 cut(s) 235, 784, 1003, 1057, 1279
BmuI ACTGGG 2 cut(s) 1103, 1511
BpiI GAAGAC 1 cut(s) 1082
Bpu10I CCTNAGC 1 cut(s) 540
BpuEI CTTGAG 4 cut(s) 467, 984, 1315, 1442
BsaBI GATNNNNATC 1 cut(s) 1172
BsaJI CCNNGG 6 cut(s) 133, 460, 597, 660, 1476, 1490
BsaWI WCCGGW 1 cut(s) 873
Bsc4I CCNNNNNNNGG 4 cut(s) 247, 484, 1095, 1495
Bse1I ACTGG 4 cut(s) 406, 830, 1098, 1517
Bse3DI GCAATG 1 cut(s) 844
Bse8I GATNNNNATC 1 cut(s) 1172
BseAI TCCGGA 1 cut(s) 873
BseBI CCWGG 2 cut(s) 272, 529
BseDI CCNNGG 6 cut(s) 133, 460, 597, 660, 1476, 1490
BseGI GGATG 7 cut(s) 728, 775, 882, 1185, 1255, 1308, 1500
BseJI GATNNNNATC 1 cut(s) 1172
BseLI CCNNNNNNNGG 4 cut(s) 247, 484, 1095, 1495
BseMI GCAATG 1 cut(s) 844
BseMII CTCAG 2 cut(s) 531, 927
BseNI ACTGG 4 cut(s) 406, 830, 1098, 1517
BseXI GCAGC 5 cut(s) 163, 639, 874, 1113, 1135
BseYI CCCAGC 3 cut(s) 180, 1016, 1229
Bsh1236I CGCG 1 cut(s) 731
BshFI GGCC 2 cut(s) 459, 1099
BshNI GGYRCC 1 cut(s) 557
BsiSI CCGG 2 cut(s) 456, 874
BslI CCNNNNNNNGG 4 cut(s) 247, 484, 1095, 1495
BsmI GAATGC 1 cut(s) 1221
BsnI GGCC 2 cut(s) 459, 1099
Bsp1286I GDGCHC 2 cut(s) 738, 1490
Bsp13I TCCGGA 1 cut(s) 873
Bsp19I CCATGG 2 cut(s) 133, 660
BspACI CCGC 4 cut(s) 5, 304, 731, 955
BspANI GGCC 2 cut(s) 459, 1099
BspCNI CTCAG 2 cut(s) 532, 926
BspEI TCCGGA 1 cut(s) 873
BspFNI CGCG 1 cut(s) 731
BspLI GGNNCC 6 cut(s) 9, 65, 443, 559, 1098, 1293
BspMAI CTGCAG 1 cut(s) 629
BspMI ACCTGC 2 cut(s) 1052, 1275
BspPI GGATC 6 cut(s) 25, 393, 436, 447, 449, 1463
BspQI GCTCTTC 1 cut(s) 1231
BspT107I GGYRCC 1 cut(s) 557
BsrDI GCAATG 1 cut(s) 844
BsrI ACTGG 4 cut(s) 406, 830, 1098, 1517
BssECI CCNNGG 6 cut(s) 133, 460, 597, 660, 1476, 1490
BssNAI GTATAC 1 cut(s) 292
BssT1I CCWWGG 5 cut(s) 133, 460, 597, 660, 1476
Bst1107I GTATAC 1 cut(s) 292
Bst2UI CCWGG 2 cut(s) 272, 529
Bst4CI ACNGT 5 cut(s) 289, 429, 952, 1161, 1439
Bst6I CTCTTC 2 cut(s) 411, 1231
BstAPI GCANNNNNTGC 1 cut(s) 1067
BstC8I GCNNGC 1 cut(s) 959
BstDEI CTNAG 2 cut(s) 540, 913
BstDSI CCRYGG 3 cut(s) 133, 660, 1490
BstEII GGTNACC 1 cut(s) 113
BstENI CCTNNNNNAGG 1 cut(s) 482
BstF5I GGATG 7 cut(s) 728, 775, 882, 1185, 1255, 1308, 1500
BstFNI CGCG 1 cut(s) 731
BstHHI GCGC 2 cut(s) 731, 793
BstMWI GCNNNNNNNGC 2 cut(s) 1067, 1291
BstNI CCWGG 2 cut(s) 272, 529
BstPI GGTNACC 1 cut(s) 113
BstSCI CCNGG 2 cut(s) 270, 527
BstSFI CTRYAG 4 cut(s) 425, 625, 1056, 1464
BstUI CGCG 1 cut(s) 731
BstV1I GCAGC 5 cut(s) 163, 639, 874, 1113, 1135
BstV2I GAAGAC 1 cut(s) 1082
BstX2I RGATCY 4 cut(s) 205, 441, 452, 1468
BstYI RGATCY 4 cut(s) 205, 441, 452, 1468
BstZ17I GTATAC 1 cut(s) 292
BsuRI GGCC 2 cut(s) 459, 1099
BtgI CCRYGG 3 cut(s) 133, 660, 1490
BtsCI GGATG 7 cut(s) 728, 775, 882, 1185, 1255, 1308, 1500
BtsI GCAGTG 1 cut(s) 552
BtsIMutI CAGTG 4 cut(s) 285, 552, 1091, 1444
BveI ACCTGC 2 cut(s) 1052, 1275
Cac8I GCNNGC 1 cut(s) 959
CaiI CAGNNNCTG 1 cut(s) 488
CfoI GCGC 2 cut(s) 731, 793
Cfr13I GGNCC 3 cut(s) 927, 1045, 1097
Csp6I GTAC 6 cut(s) 142, 319, 405, 491, 829, 934
CspCI CAANNNNNGTGG 2 cut(s) 694, 729
CviAII CATG 6 cut(s) 134, 382, 661, 805, 1226, 1401
CviQI GTAC 6 cut(s) 142, 319, 405, 491, 829, 934
DdeI CTNAG 2 cut(s) 540, 913
Eam1104I CTCTTC 2 cut(s) 411, 1231
EarI CTCTTC 2 cut(s) 411, 1231
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 5 cut(s) 133, 460, 597, 660, 1476
Eco24I GRGCYC 2 cut(s) 738, 1490
Eco47I GGWCC 2 cut(s) 927, 1045
Eco57I CTGAAG 1 cut(s) 752
Eco91I GGTNACC 1 cut(s) 113
EcoNI CCTNNNNNAGG 1 cut(s) 482
EcoO109I RGGNCCY 2 cut(s) 1045, 1097
EcoO65I GGTNACC 1 cut(s) 113
EcoRII CCWGG 2 cut(s) 270, 527
EcoT14I CCWWGG 5 cut(s) 133, 460, 597, 660, 1476
EcoT38I GRGCYC 2 cut(s) 738, 1490
ErhI CCWWGG 5 cut(s) 133, 460, 597, 660, 1476
FaeI CATG 6 cut(s) 137, 385, 664, 808, 1229, 1404
FatI CATG 6 cut(s) 133, 381, 660, 804, 1225, 1400
FauI CCCGC 2 cut(s) 311, 724
FauNDI CATATG 1 cut(s) 1287
FbaI TGATCA 2 cut(s) 252, 1507
FblI GTMKAC 2 cut(s) 291, 392
Fnu4HI GCNGC 5 cut(s) 177, 628, 888, 1124, 1127
FokI GGATG 7 cut(s) 735, 762, 889, 1172, 1262, 1315, 1507
FriOI GRGCYC 2 cut(s) 738, 1490
Fsp4HI GCNGC 5 cut(s) 177, 628, 888, 1124, 1127
FspBI CTAG 1 cut(s) 45
GlaI GCGC 2 cut(s) 730, 792
GluI GCNGC 5 cut(s) 177, 628, 888, 1124, 1127
GsaI CCCAGC 3 cut(s) 184, 1020, 1233
HaeIII GGCC 2 cut(s) 459, 1099
HapII CCGG 2 cut(s) 456, 874
HhaI GCGC 2 cut(s) 731, 793
Hin1II CATG 6 cut(s) 137, 385, 664, 808, 1229, 1404
Hin6I GCGC 2 cut(s) 729, 791
HinP1I GCGC 2 cut(s) 729, 791
HincII GTYRAC 2 cut(s) 328, 622
HindII GTYRAC 2 cut(s) 328, 622
HindIII AAGCTT 3 cut(s) 107, 761, 851
HinfI GANTC 4 cut(s) 421, 467, 609, 1425
HpaI GTTAAC 1 cut(s) 328
HpaII CCGG 2 cut(s) 456, 874
HphI GGTGA 5 cut(s) 121, 262, 476, 1076, 1078
Hpy166II GTNNAC 5 cut(s) 292, 319, 328, 393, 622
Hpy188I TCNGA 5 cut(s) 608, 916, 1120, 1199, 1279
Hpy188III TCNNGA 9 cut(s) 45, 433, 482, 613, 874, 1001, 1165, 1422, 1459
Hpy8I GTNNAC 5 cut(s) 292, 319, 328, 393, 622
HpyAV CCTTC 5 cut(s) 432, 488, 915, 1036, 1371
HpyCH4III ACNGT 5 cut(s) 289, 429, 952, 1161, 1439
HpyCH4V TGCA 5 cut(s) 366, 627, 775, 1221, 1270
HpyF10VI GCNNNNNNNGC 2 cut(s) 1067, 1291
HpyF3I CTNAG 2 cut(s) 540, 913
Hsp92II CATG 6 cut(s) 137, 385, 664, 808, 1229, 1404
HspAI GCGC 2 cut(s) 729, 791
Kpn2I TCCGGA 1 cut(s) 873
Ksp22I TGATCA 2 cut(s) 252, 1507
KspAI GTTAAC 1 cut(s) 328
LguI GCTCTTC 1 cut(s) 1231
LmnI GCTCC 3 cut(s) 204, 223, 1291
Lsp1109I GCAGC 5 cut(s) 163, 639, 874, 1113, 1135
LweI GCATC 5 cut(s) 235, 784, 1003, 1057, 1279
MaeI CTAG 1 cut(s) 45
MaeIII GTNAC 6 cut(s) 113, 338, 994, 1030, 1433, 1534
MfeI CAATTG 1 cut(s) 744
MflI RGATCY 4 cut(s) 205, 441, 452, 1468
MhlI GDGCHC 2 cut(s) 738, 1490
MluCI AATT 5 cut(s) 744, 862, 938, 1253, 1503
MmeI TCCRAC 1 cut(s) 665
MroI TCCGGA 1 cut(s) 873
MroXI GAANNNNTTC 1 cut(s) 1160
MseI TTAA 4 cut(s) 90, 327, 495, 1383
MslI CAYNNNNRTG 2 cut(s) 966, 1026
MspA1I CMGCKG 1 cut(s) 1126
MspI CCGG 2 cut(s) 456, 874
MspR9I CCNGG 2 cut(s) 272, 529
MunI CAATTG 1 cut(s) 744
Mva1269I GAATGC 1 cut(s) 1221
MvaI CCWGG 2 cut(s) 272, 529
MvnI CGCG 1 cut(s) 731
MwoI GCNNNNNNNGC 2 cut(s) 1067, 1291
NcoI CCATGG 2 cut(s) 133, 660
NdeI CATATG 1 cut(s) 1287
NlaIII CATG 6 cut(s) 137, 385, 664, 808, 1229, 1404
NlaIV GGNNCC 6 cut(s) 9, 65, 443, 559, 1098, 1293
NmuCI GTSAC 2 cut(s) 1030, 1534
OliI CACNNNNGTG 1 cut(s) 966
PaqCI CACCTGC 1 cut(s) 1052
PciSI GCTCTTC 1 cut(s) 1231
PctI GAATGC 1 cut(s) 1221
PdmI GAANNNNTTC 1 cut(s) 1160
PfeI GAWTC 4 cut(s) 421, 467, 609, 1425
PkrI GCNGC 5 cut(s) 178, 629, 889, 1125, 1128
PpuMI RGGWCCY 1 cut(s) 1045
Psp5II RGGWCCY 1 cut(s) 1045
Psp6I CCWGG 2 cut(s) 270, 527
PspEI GGTNACC 1 cut(s) 113
PspFI CCCAGC 3 cut(s) 180, 1016, 1229
PspGI CCWGG 2 cut(s) 270, 527
PspN4I GGNNCC 6 cut(s) 9, 65, 443, 559, 1098, 1293
PspPI GGNCC 3 cut(s) 927, 1045, 1097
PspPPI RGGWCCY 1 cut(s) 1045
PstI CTGCAG 1 cut(s) 629
PstNI CAGNNNCTG 1 cut(s) 488
PsuI RGATCY 4 cut(s) 205, 441, 452, 1468
PvuII CAGCTG 1 cut(s) 1126
RsaI GTAC 6 cut(s) 143, 320, 406, 492, 830, 935
RsaNI GTAC 6 cut(s) 142, 319, 405, 491, 829, 934
RseI CAYNNNNRTG 2 cut(s) 966, 1026
SapI GCTCTTC 1 cut(s) 1231
SaqAI TTAA 4 cut(s) 90, 327, 495, 1383
SatI GCNGC 5 cut(s) 177, 628, 888, 1124, 1127
Sau96I GGNCC 3 cut(s) 927, 1045, 1097
ScrFI CCNGG 2 cut(s) 272, 529
SduI GDGCHC 2 cut(s) 738, 1490
SfaNI GCATC 5 cut(s) 235, 784, 1003, 1057, 1279
SfcI CTRYAG 4 cut(s) 425, 625, 1056, 1464
SinI GGWCC 2 cut(s) 927, 1045
SmiMI CAYNNNNRTG 2 cut(s) 966, 1026
SmlI CTYRAG 4 cut(s) 446, 999, 1330, 1457
SmoI CTYRAG 4 cut(s) 446, 999, 1330, 1457
Sse9I AATT 5 cut(s) 744, 862, 938, 1253, 1503
SsiI CCGC 4 cut(s) 5, 304, 731, 955
SspMI CTAG 1 cut(s) 45
StyD4I CCNGG 2 cut(s) 270, 527
StyI CCWWGG 5 cut(s) 133, 460, 597, 660, 1476
TaaI ACNGT 5 cut(s) 289, 429, 952, 1161, 1439
TaqI TCGA 4 cut(s) 263, 358, 685, 1040
TasI AATT 5 cut(s) 744, 862, 938, 1253, 1503
TatI WGTACW 1 cut(s) 490
TfiI GAWTC 4 cut(s) 421, 467, 609, 1425
Tru1I TTAA 4 cut(s) 90, 327, 495, 1383
Tru9I TTAA 4 cut(s) 90, 327, 495, 1383
TscAI CASTG 4 cut(s) 292, 552, 1098, 1444
TseFI GTSAC 2 cut(s) 1030, 1534
TseI GCWGC 5 cut(s) 176, 627, 887, 1123, 1126
Tsp45I GTSAC 2 cut(s) 1030, 1534
TspDTI ATGAA 5 cut(s) 370, 533, 1184, 1214, 1266
TspGWI ACGGA 1 cut(s) 501
TspRI CASTG 4 cut(s) 292, 552, 1098, 1444
VpaK11BI GGWCC 2 cut(s) 927, 1045
XagI CCTNNNNNAGG 1 cut(s) 482
XbaI TCTAGA 1 cut(s) 44
XmiI GTMKAC 2 cut(s) 291, 392
XmnI GAANNNNTTC 1 cut(s) 1160
XspI CTAG 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.