pycom15g16040

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003748
Physical Location & Seq
Reverse (-)
11172938 .. 11174353
1416 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g16040.1

Sequence Viewer

Length: 1416 bp
ATGTACCTCTTTCTTTCCCCTCTTGTTGTCGTTATTGCTGCCCAGATTTCGACATTTTCTCTCAAGGATCTCTATGATCTTTGGGAGCATCTCCAGTACAACCTGATTTCAGTGATTATCTGCTCTACTCTCCTTGTTTTCCTGTCCACTGTCTACTTTGTAACCCGACCTCGCCCGGTGTACCTTGTTAATTTTTCCTGCTACAAACCAGAAGAATCTCGAAAGTGCACGAAAAAGATTTTCATGGAACAGTCCCAGATGACTGGTACATTTACAGAGGACAATCTTCAGTTCCAGCGAAGGATCCTTGAGAGATCCGGCCTTGGTGATTCGACTTACCTTCCAGAGGCTGTACTCAACATTCCTCCAAATCCATCAATGAAAGAAGCTAGAAAAGAAGCCGAGGCTGTGATGTTTGGTGCCATTGATGAGCTTTTGGCTAAGACAGCTGTAAAACCTAAGGACATTGGAATCTTGATTGTGAACTGCAGCTTGTTCAATCCAACCCCATCTCTTTCTGCCATGGTTATCAACCATTACCAGCTTCGAGGGAACATAGTCAGCTACAATCTTGGTGGGATGGGATGCAGTGCAGGTCTGATCGATCAACAAGTTGATCTTGCTAAAAATCTTCTTCAGGTGCATCCCAACTCTTATGCACTGGTTATCAGCATGGAGAACATCACCTTGAACTGGTACTTTGGAAACGACCGCTCAAAGCTTGTCTCAAATTGCTTATTCCGTATGGGAGGGGCTGCTGTATTGCTTTCAAACAAAAGCTCGGACAAAAGAAGGTCCAAGTACCGATTGGTTCATACAGTTCGTACCCACAAGGGTGCTGATGATAAGTGCTTTAGCTGCGTTACCCAAGAAGAAGATGATAATGGGAAGATTGGTGTTACTTTATCAAAGGATCGGATGGCAGTAGCAGGTGATGCTCTAAAGACCAACATCACCACATTGGGGCCTCTTGTGCTGCCAATGTCCGAACAGCTGCTTTTCTTTGCTACATTGGTTGGGAGGAAACTGTTCAAGATGAAGATCAAACCATACATCCCAGATTTCAGACTGGCTTTTGAGCATTTCTGTATTCATGCTGGAGGAAGAGCTGTCTTGGACGAATTGGAGAAGAACCTTCATCTATCAGATTGGCATATGGAGCCATCGAGGATGACACTTTATCGATTTGGAAACACCTCAAGCAGCTCTCTTTGGTATGAACTAGCTTACACAGAAGCCAAGGGAAGGATGAAGAAAGGACATAGGACATGGCAGATAGCTTTCGGGTCGGGATTCAAGTGCAACAGTGCTGTGTGGAAGGCTCTTAGGAGCATAAACCCCGCAAAGGAAAAGAACCCGTGGATGGAAGAAATTGATCGGTTCCCAGTCGATGTTCCAAGGGTGTCAACCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

472

Amino Acids

53.31

Weight (kDa)

9.1

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 52 - 343 2.4e-141 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 360 - 440 6.3e-09 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 362 - 438 1.2e-08 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 920
Acc36I ACCTGC 2 cut(s) 584, 920
AccB1I GGYRCC 1 cut(s) 419
AccBSI CCGCTC 1 cut(s) 714
AccI GTMKAC 1 cut(s) 153
AciI CCGC 2 cut(s) 712, 1341
AclWI GGATC 5 cut(s) 75, 298, 309, 311, 921
AcuI CTGAAG 2 cut(s) 272, 620
AfaI GTAC 8 cut(s) 5, 98, 182, 268, 354, 698, 803, 826
AfiI CCNNNNNNNGG 6 cut(s) 346, 693, 963, 1245, 1345, 1363
AgsI TTSAA 5 cut(s) 499, 691, 771, 1033, 1297
AleI CACNNNNGTG 1 cut(s) 834
Alw21I GWGCWC 1 cut(s) 230
Alw26I GTCTC 1 cut(s) 730
Alw44I GTGCAC 1 cut(s) 226
AlwI GGATC 5 cut(s) 75, 298, 309, 311, 921
AlwNI CAGNNNCTG 1 cut(s) 350
AoxI GGCC 2 cut(s) 319, 965
ApaLI GTGCAC 1 cut(s) 226
ApeKI GCWGC 7 cut(s) 38, 489, 755, 858, 976, 994, 1203
Asp700I GAANNNNTTC 1 cut(s) 1028
AspS9I GGNCC 2 cut(s) 795, 965
AsuC2I CCSGG 1 cut(s) 176
AsuHPI GGTGA 4 cut(s) 338, 676, 944, 946
AvaII GGWCC 1 cut(s) 795
AxyI CCTNAGG 1 cut(s) 459
BaeGI GKGCMC 1 cut(s) 230
BamHI GGATCC 1 cut(s) 303
BanI GGYRCC 1 cut(s) 419
Bbv12I GWGCWC 1 cut(s) 230
BbvI GCAGC 7 cut(s) 25, 501, 742, 845, 963, 981, 1215
BccI CCATC 6 cut(s) 382, 517, 574, 913, 1171, 1357
BcnI CCSGG 1 cut(s) 176
BcoDI GTCTC 1 cut(s) 730
BfaI CTAG 2 cut(s) 390, 1223
BfmI CTRYAG 1 cut(s) 487
BfuAI ACCTGC 2 cut(s) 584, 920
BisI GCNGC 7 cut(s) 39, 490, 756, 859, 977, 995, 1204
BlsI GCNGC 7 cut(s) 40, 491, 757, 860, 978, 996, 1205
Bme1390I CCNGG 1 cut(s) 176
Bme18I GGWCC 1 cut(s) 795
BmgT120I GGNCC 2 cut(s) 795, 965
BmiI GGNNCC 5 cut(s) 305, 421, 966, 1161, 1382
BmrFI CCNGG 1 cut(s) 176
BmrI ACTGGG 1 cut(s) 1379
BmsI GCATC 4 cut(s) 97, 575, 652, 925
BmuI ACTGGG 1 cut(s) 1379
BpmI CTGGAG 2 cut(s) 77, 1119
BpuEI CTTGAG 3 cut(s) 47, 329, 1183
BpuMI CCSGG 1 cut(s) 176
Bsa29I ATCGAT 2 cut(s) 603, 1183
BsaBI GATNNNNATC 1 cut(s) 1040
BsaJI CCNNGG 6 cut(s) 322, 402, 522, 1239, 1358, 1397
Bsc4I CCNNNNNNNGG 6 cut(s) 346, 693, 963, 1245, 1345, 1363
Bse1I ACTGG 6 cut(s) 94, 268, 666, 698, 1074, 1385
Bse21I CCTNAGG 1 cut(s) 459
Bse8I GATNNNNATC 1 cut(s) 1040
BseCI ATCGAT 2 cut(s) 603, 1183
BseDI CCNNGG 6 cut(s) 322, 402, 522, 1239, 1358, 1397
BseGI GGATG 8 cut(s) 585, 590, 643, 924, 1053, 1176, 1254, 1368
BseJI GATNNNNATC 1 cut(s) 1040
BseLI CCNNNNNNNGG 6 cut(s) 346, 693, 963, 1245, 1345, 1363
BseNI ACTGG 6 cut(s) 94, 268, 666, 698, 1074, 1385
BseSI GKGCMC 1 cut(s) 230
BseXI GCAGC 7 cut(s) 25, 501, 742, 845, 963, 981, 1215
BsgI GTGCAG 1 cut(s) 612
Bsh1285I CGRYCG 1 cut(s) 712
BshFI GGCC 2 cut(s) 321, 967
BshNI GGYRCC 1 cut(s) 419
BshVI ATCGAT 2 cut(s) 603, 1183
BsiEI CGRYCG 1 cut(s) 712
BsiHKAI GWGCWC 1 cut(s) 230
BsiSI CCGG 2 cut(s) 176, 318
BslFI GGGAC 1 cut(s) 238
BslI CCNNNNNNNGG 6 cut(s) 346, 693, 963, 1245, 1345, 1363
BsmAI GTCTC 1 cut(s) 730
BsmFI GGGAC 1 cut(s) 238
BsnI GGCC 2 cut(s) 321, 967
Bsp1286I GDGCHC 1 cut(s) 230
Bsp19I CCATGG 1 cut(s) 522
BspACI CCGC 2 cut(s) 712, 1341
BspANI GGCC 2 cut(s) 321, 967
BspDI ATCGAT 2 cut(s) 603, 1183
BspLI GGNNCC 5 cut(s) 305, 421, 966, 1161, 1382
BspMAI CTGCAG 1 cut(s) 491
BspMI ACCTGC 2 cut(s) 584, 920
BspPI GGATC 5 cut(s) 75, 298, 309, 311, 921
BspQI GCTCTTC 1 cut(s) 1099
BspT107I GGYRCC 1 cut(s) 419
BsrBI CCGCTC 1 cut(s) 714
BsrI ACTGG 6 cut(s) 94, 268, 666, 698, 1074, 1385
BssECI CCNNGG 6 cut(s) 322, 402, 522, 1239, 1358, 1397
BssT1I CCWWGG 4 cut(s) 322, 522, 1239, 1397
Bst4CI ACNGT 5 cut(s) 151, 252, 820, 1029, 1307
Bst6I CTCTTC 1 cut(s) 1099
BstAPI GCANNNNNTGC 1 cut(s) 935
BstDEI CTNAG 3 cut(s) 441, 459, 1325
BstDSI CCRYGG 2 cut(s) 522, 1358
BstENI CCTNNNNNAGG 1 cut(s) 344
BstF5I GGATG 8 cut(s) 585, 590, 643, 924, 1053, 1176, 1254, 1368
BstMAI GTCTC 1 cut(s) 730
BstMCI CGRYCG 1 cut(s) 712
BstMWI GCNNNNNNNGC 5 cut(s) 446, 858, 935, 973, 1159
BstSCI CCNGG 1 cut(s) 174
BstSFI CTRYAG 1 cut(s) 487
BstSLI GKGCMC 1 cut(s) 230
BstV1I GCAGC 7 cut(s) 25, 501, 742, 845, 963, 981, 1215
BstX2I RGATCY 3 cut(s) 67, 303, 314
BstXI CCANNNNNNTGG 1 cut(s) 263
BstYI RGATCY 3 cut(s) 67, 303, 314
Bsu15I ATCGAT 2 cut(s) 603, 1183
Bsu36I CCTNAGG 1 cut(s) 459
BsuRI GGCC 2 cut(s) 321, 967
BsuTUI ATCGAT 2 cut(s) 603, 1183
BtgI CCRYGG 2 cut(s) 522, 1358
BtsCI GGATG 8 cut(s) 585, 590, 643, 924, 1053, 1176, 1254, 1368
BtsI GCAGTG 1 cut(s) 595
BtsIMutI CAGTG 5 cut(s) 117, 147, 595, 659, 1312
BveI ACCTGC 2 cut(s) 584, 920
CaiI CAGNNNCTG 1 cut(s) 350
Cfr13I GGNCC 2 cut(s) 795, 965
ClaI ATCGAT 2 cut(s) 603, 1183
Csp6I GTAC 8 cut(s) 4, 97, 181, 267, 353, 697, 802, 825
CspCI CAANNNNNGTGG 2 cut(s) 556, 591
CviAII CATG 5 cut(s) 244, 523, 673, 1094, 1269
CviQI GTAC 8 cut(s) 4, 97, 181, 267, 353, 697, 802, 825
DdeI CTNAG 3 cut(s) 441, 459, 1325
Eam1104I CTCTTC 1 cut(s) 1099
EarI CTCTTC 1 cut(s) 1099
Eco130I CCWWGG 4 cut(s) 322, 522, 1239, 1397
Eco47I GGWCC 1 cut(s) 795
Eco57I CTGAAG 2 cut(s) 272, 620
Eco81I CCTNAGG 1 cut(s) 459
EcoNI CCTNNNNNAGG 1 cut(s) 344
EcoO109I RGGNCCY 1 cut(s) 965
EcoT14I CCWWGG 4 cut(s) 322, 522, 1239, 1397
ErhI CCWWGG 4 cut(s) 322, 522, 1239, 1397
FaeI CATG 5 cut(s) 247, 526, 676, 1097, 1272
FalI AAGNNNNNCTT 2 cut(s) 603, 635
FaqI GGGAC 1 cut(s) 238
FatI CATG 5 cut(s) 243, 522, 672, 1093, 1268
FauI CCCGC 1 cut(s) 1348
FauNDI CATATG 1 cut(s) 1155
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 7 cut(s) 39, 490, 756, 859, 977, 995, 1204
FokI GGATG 8 cut(s) 592, 597, 630, 931, 1040, 1183, 1261, 1375
Fsp4HI GCNGC 7 cut(s) 39, 490, 756, 859, 977, 995, 1204
FspBI CTAG 2 cut(s) 390, 1223
GluI GCNGC 7 cut(s) 39, 490, 756, 859, 977, 995, 1204
GsuI CTGGAG 2 cut(s) 77, 1119
HaeIII GGCC 2 cut(s) 321, 967
HapII CCGG 2 cut(s) 176, 318
Hin1II CATG 5 cut(s) 247, 526, 676, 1097, 1272
HincII GTYRAC 1 cut(s) 1407
HindII GTYRAC 1 cut(s) 1407
HindIII AAGCTT 1 cut(s) 719
HinfI GANTC 4 cut(s) 215, 329, 471, 1293
HpaII CCGG 2 cut(s) 176, 318
HphI GGTGA 4 cut(s) 338, 676, 944, 946
Hpy166II GTNNAC 6 cut(s) 147, 154, 181, 228, 484, 1407
Hpy188I TCNGA 6 cut(s) 600, 784, 918, 988, 1067, 1147
Hpy188III TCNNGA 5 cut(s) 219, 344, 475, 1033, 1290
Hpy8I GTNNAC 6 cut(s) 147, 154, 181, 228, 484, 1407
HpyAV CCTTC 6 cut(s) 294, 350, 786, 1145, 1239, 1312
HpyCH4III ACNGT 5 cut(s) 151, 252, 820, 1029, 1307
HpyCH4V TGCA 7 cut(s) 228, 489, 588, 593, 643, 659, 1302
HpyF10VI GCNNNNNNNGC 5 cut(s) 446, 858, 935, 973, 1159
HpyF3I CTNAG 3 cut(s) 441, 459, 1325
Hsp92II CATG 5 cut(s) 247, 526, 676, 1097, 1272
LguI GCTCTTC 1 cut(s) 1099
LmnI GCTCC 3 cut(s) 85, 1159, 1329
Lsp1109I GCAGC 7 cut(s) 25, 501, 742, 845, 963, 981, 1215
LweI GCATC 4 cut(s) 97, 575, 652, 925
MaeI CTAG 2 cut(s) 390, 1223
MaeIII GTNAC 3 cut(s) 160, 862, 898
MbiI CCGCTC 1 cut(s) 714
MflI RGATCY 3 cut(s) 67, 303, 314
MhlI GDGCHC 1 cut(s) 230
MluCI AATT 4 cut(s) 190, 730, 1121, 1371
MmeI TCCRAC 1 cut(s) 527
MroXI GAANNNNTTC 1 cut(s) 1028
MseI TTAA 1 cut(s) 189
MslI CAYNNNNRTG 1 cut(s) 834
MspA1I CMGCKG 2 cut(s) 449, 994
MspI CCGG 2 cut(s) 176, 318
MspR9I CCNGG 1 cut(s) 176
MwoI GCNNNNNNNGC 5 cut(s) 446, 858, 935, 973, 1159
NciI CCSGG 1 cut(s) 176
NcoI CCATGG 1 cut(s) 522
NdeI CATATG 1 cut(s) 1155
NlaIII CATG 5 cut(s) 247, 526, 676, 1097, 1272
NlaIV GGNNCC 5 cut(s) 305, 421, 966, 1161, 1382
NmeAIII GCCGAG 1 cut(s) 427
OliI CACNNNNGTG 1 cut(s) 834
PaqCI CACCTGC 1 cut(s) 920
PciSI GCTCTTC 1 cut(s) 1099
PdmI GAANNNNTTC 1 cut(s) 1028
PfeI GAWTC 4 cut(s) 215, 329, 471, 1293
PkrI GCNGC 7 cut(s) 40, 491, 757, 860, 978, 996, 1205
PspN4I GGNNCC 5 cut(s) 305, 421, 966, 1161, 1382
PspPI GGNCC 2 cut(s) 795, 965
PstI CTGCAG 1 cut(s) 491
PstNI CAGNNNCTG 1 cut(s) 350
PsuI RGATCY 3 cut(s) 67, 303, 314
PvuII CAGCTG 2 cut(s) 449, 994
RsaI GTAC 8 cut(s) 5, 98, 182, 268, 354, 698, 803, 826
RsaNI GTAC 8 cut(s) 4, 97, 181, 267, 353, 697, 802, 825
RseI CAYNNNNRTG 1 cut(s) 834
SapI GCTCTTC 1 cut(s) 1099
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 7 cut(s) 39, 490, 756, 859, 977, 995, 1204
Sau96I GGNCC 2 cut(s) 795, 965
ScrFI CCNGG 1 cut(s) 176
SduI GDGCHC 1 cut(s) 230
SfaNI GCATC 4 cut(s) 97, 575, 652, 925
SfcI CTRYAG 1 cut(s) 487
SinI GGWCC 1 cut(s) 795
SmiMI CAYNNNNRTG 1 cut(s) 834
SmlI CTYRAG 3 cut(s) 62, 308, 1198
SmoI CTYRAG 3 cut(s) 62, 308, 1198
Sse9I AATT 4 cut(s) 190, 730, 1121, 1371
SsiI CCGC 2 cut(s) 712, 1341
SspMI CTAG 2 cut(s) 390, 1223
StyD4I CCNGG 1 cut(s) 174
StyI CCWWGG 4 cut(s) 322, 522, 1239, 1397
TaaI ACNGT 5 cut(s) 151, 252, 820, 1029, 1307
TaqI TCGA 8 cut(s) 50, 220, 332, 547, 603, 1166, 1183, 1389
TasI AATT 4 cut(s) 190, 730, 1121, 1371
TatI WGTACW 2 cut(s) 96, 352
TfiI GAWTC 4 cut(s) 215, 329, 471, 1293
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TscAI CASTG 5 cut(s) 117, 154, 595, 666, 1312
TseI GCWGC 7 cut(s) 38, 489, 755, 858, 976, 994, 1203
TspDTI ATGAA 8 cut(s) 232, 395, 803, 1052, 1082, 1127, 1233, 1265
TspGWI ACGGA 1 cut(s) 731
TspRI CASTG 5 cut(s) 117, 154, 595, 666, 1312
VneI GTGCAC 1 cut(s) 226
VpaK11BI GGWCC 1 cut(s) 795
XagI CCTNNNNNAGG 1 cut(s) 344
XcmI CCANNNNNNNNNTGG 1 cut(s) 805
XmiI GTMKAC 1 cut(s) 153
XmnI GAANNNNTTC 1 cut(s) 1028
XspI CTAG 2 cut(s) 390, 1223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.