Rroxscaffold_2G00151880

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003748
Physical Location & Seq
Forward (+)
89010740 .. 89012287
1548 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00151880.1

Sequence Viewer

Length: 1548 bp
ATGGCGGAGCCAAAACCAGATACACCCTTGATCCCACCATCATCTACACCCTCATCCAATCGGAACCTCCCCGACTTCAAAAAATCCATTAAGCTAAAATATGTGAAGCTTGGTTACCATATTCTCATCACCCATGGAATGTACCTTTTTCTTTCCCCTCTCGTGGTTGTTATTGCTGCCCAACTCTCAACATTTTCGCTCCAAGATCTTTATGATCTTTGGGAGCATCTCCAATACAACCTGATTTCGGTGATTATCTGCTCGACCCTCCTGGTGTTCCTATCCACTGTCTACTTTCTTACCCGCCCTCGCCCTGTGTACCTTGTTAACTTCTCCTGTTACAAGCCAGAAGAAACTCGAAAATGCACCAAAAAGATCTTTATGGAACAGTCTAAGATGACTGGTACATTCTCAGAGGAGAATCTACAGTTCCAGAGAAGGATCCTTGAGAGATCCGGCCTTGGTGATTCAACATACCTTCCAGAGGCTGTACTCAACATTCCTCCAAATCCGTCAATGAAAGAAGCCAGAAAAGAAGCTGAGGCAGTGATGTTTGGTGCCATTGACGAACTTTTTGCCAAGACATCTGTAAAACCCAAGGACATTGGAATATTGATTGTCAACTGCAGTTTGTTCAATCCAACTCCATCTCTTTCTGCCATGGTTATCAACCATTACCAGCTTCGAGGGAACATAGTCAGCTACAATCTTGGTGGAATGGGATGCAGCGCAGGTCTCATCTCAATTGATCTTGCTCAAAAGCTTCTTCAGGTGCATCCTAACTCTTATGCGCTGGTTATCAGCATGGAGAACATCACATTGAACTGGTACTTTGGGAATGATCGCTCAAAGCTTGTTTCAAATTGCTTATTTCGGATGGGAGGAGCTGCTATACTTCTTTCAAACAAAAGCTCAGACAGAAGAAGGTCCAAATACCGATTGGTTCAAACTGTCCGCACGCACAAGGGTGCTGATGACAAGTGCTTTAGCTGTGTTACCCAAGAAGAAGATGATGCTGGGAAGATTGGTGTTACTCTATCAAAGGACCTGATGGCTGTAGCAGGTGATGCTTTGAAGACCAATATCACCACATTGGGGCCTCTTGTTCTTCCAATGTCCGAGCAGCTGCTTTTCTTTGCTACATTGGTGGGTAGGAAACTCTTCAAGATGAAGATCAAACCTTACATCCCAGATTTCAGACTTGCTTTTGAGCATTTCTGCATTCATGCTGGAGGAAGAGCCGTCTTGGATGAATTAGAGAAGAACCTGCATCTATCTGATTGGCATATGGAGCCATCAAGGATGACACTATACCGATTTGGAAACACCTCAAGCAGTTCTCTCTGGTATGAGTTGGCTTACACAGAAGCAAAGGGAAGGATCAAGAAAGGAGATAGAACATGGCAGATAGCTTTTGGGTCGGGATTCAAGTGTAATAGTGCTGTGTGGAAAGCTCTCAAGAGCATAGATCCTGCAAAGGAAAAGAGCCCATGGATGGCTGAAATTGATCAGTTCCCAGTCGATGTTCCAAGGGTGTCAACCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

515

Amino Acids

58.01

Weight (kDa)

9.16

Isoelectric Point (pI)

41.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 98 - 387 8.8e-145 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 404 - 484 7.9e-10 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 406 - 482 1.5e-08 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1052
Acc36I ACCTGC 3 cut(s) 722, 1052, 1275
AccB1I GGYRCC 1 cut(s) 557
AccI GTMKAC 1 cut(s) 291
AciI CCGC 3 cut(s) 5, 304, 955
AclWI GGATC 6 cut(s) 25, 436, 447, 449, 1388, 1463
AcuI CTGAAG 1 cut(s) 752
AfaI GTAC 5 cut(s) 143, 320, 406, 492, 830
AfiI CCNNNNNNNGG 5 cut(s) 163, 247, 484, 1095, 1495
AjnI CCWGG 1 cut(s) 270
AleI CACNNNNGTG 1 cut(s) 966
Alw26I GTCTC 1 cut(s) 740
AlwI GGATC 6 cut(s) 25, 436, 447, 449, 1388, 1463
AlwNI CAGNNNCTG 1 cut(s) 488
AoxI GGCC 2 cut(s) 457, 1097
ApeKI GCWGC 5 cut(s) 176, 726, 887, 1123, 1126
ArsI GACNNNNNNTTYG 2 cut(s) 557, 589
Asp700I GAANNNNTTC 1 cut(s) 1160
AspLEI GCGC 2 cut(s) 731, 793
AspS9I GGNCC 3 cut(s) 927, 1045, 1097
AsuHPI GGTGA 5 cut(s) 121, 262, 476, 1076, 1078
AvaII GGWCC 2 cut(s) 927, 1045
BamHI GGATCC 1 cut(s) 441
BanI GGYRCC 1 cut(s) 557
BanII GRGCYC 1 cut(s) 1490
BarI GAAGNNNNNNTAC 2 cut(s) 98, 130
BauI CACGAG 1 cut(s) 161
BbsI GAAGAC 1 cut(s) 1082
BbvCI CCTCAGC 1 cut(s) 540
BbvI GCAGC 5 cut(s) 163, 738, 874, 1113, 1135
BccI CCATC 6 cut(s) 46, 655, 871, 1045, 1303, 1489
BceAI ACGGC 1 cut(s) 1226
BcgI CGANNNNNNTGC 2 cut(s) 557, 591
BciT130I CCWGG 1 cut(s) 272
BclI TGATCA 1 cut(s) 1507
BcoDI GTCTC 1 cut(s) 740
BfmI CTRYAG 3 cut(s) 425, 625, 1056
BfuAI ACCTGC 3 cut(s) 722, 1052, 1275
BglII AGATCT 2 cut(s) 205, 375
BisI GCNGC 5 cut(s) 177, 727, 888, 1124, 1127
BlsI GCNGC 5 cut(s) 178, 728, 889, 1125, 1128
Bme1390I CCNGG 1 cut(s) 272
Bme18I GGWCC 2 cut(s) 927, 1045
BmgT120I GGNCC 3 cut(s) 927, 1045, 1097
BmiI GGNNCC 6 cut(s) 9, 65, 443, 559, 1098, 1293
BmrFI CCNGG 1 cut(s) 272
BmrI ACTGGG 1 cut(s) 1511
BmsI GCATC 6 cut(s) 235, 713, 784, 1003, 1057, 1279
BmuI ACTGGG 1 cut(s) 1511
BpiI GAAGAC 1 cut(s) 1082
BpmI CTGGAG 1 cut(s) 1251
Bpu10I CCTNAGC 1 cut(s) 540
BpuEI CTTGAG 3 cut(s) 467, 1315, 1442
BsaBI GATNNNNATC 1 cut(s) 1172
BsaI GGTCTC 1 cut(s) 740
BsaJI CCNNGG 6 cut(s) 133, 460, 597, 660, 1490, 1529
Bsc4I CCNNNNNNNGG 5 cut(s) 163, 247, 484, 1095, 1495
Bse1I ACTGG 3 cut(s) 406, 830, 1517
Bse8I GATNNNNATC 1 cut(s) 1172
BseBI CCWGG 1 cut(s) 272
BseDI CCNNGG 6 cut(s) 133, 460, 597, 660, 1490, 1529
BseGI GGATG 8 cut(s) 53, 728, 775, 882, 1185, 1255, 1308, 1500
BseJI GATNNNNATC 1 cut(s) 1172
BseLI CCNNNNNNNGG 5 cut(s) 163, 247, 484, 1095, 1495
BseMII CTCAG 3 cut(s) 426, 531, 927
BseNI ACTGG 3 cut(s) 406, 830, 1517
BseRI GAGGAG 2 cut(s) 431, 897
BseXI GCAGC 5 cut(s) 163, 738, 874, 1113, 1135
BseYI CCCAGC 1 cut(s) 1016
BshFI GGCC 2 cut(s) 459, 1099
BshNI GGYRCC 1 cut(s) 557
BsiSI CCGG 1 cut(s) 456
BslI CCNNNNNNNGG 5 cut(s) 163, 247, 484, 1095, 1495
BsmAI GTCTC 1 cut(s) 740
BsmI GAATGC 1 cut(s) 1221
BsnI GGCC 2 cut(s) 459, 1099
Bso31I GGTCTC 1 cut(s) 740
Bsp1286I GDGCHC 1 cut(s) 1490
Bsp19I CCATGG 3 cut(s) 133, 660, 1490
BspACI CCGC 3 cut(s) 5, 304, 955
BspANI GGCC 2 cut(s) 459, 1099
BspCNI CTCAG 3 cut(s) 425, 532, 926
BspLI GGNNCC 6 cut(s) 9, 65, 443, 559, 1098, 1293
BspMAI CTGCAG 1 cut(s) 629
BspMI ACCTGC 3 cut(s) 722, 1052, 1275
BspPI GGATC 6 cut(s) 25, 436, 447, 449, 1388, 1463
BspQI GCTCTTC 1 cut(s) 1231
BspT107I GGYRCC 1 cut(s) 557
BspTNI GGTCTC 1 cut(s) 740
BsrI ACTGG 3 cut(s) 406, 830, 1517
BssECI CCNNGG 6 cut(s) 133, 460, 597, 660, 1490, 1529
BssSI CACGAG 1 cut(s) 161
BssT1I CCWWGG 6 cut(s) 133, 460, 597, 660, 1490, 1529
Bst2BI CACGAG 1 cut(s) 161
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 4 cut(s) 289, 390, 429, 952
Bst6I CTCTTC 2 cut(s) 1166, 1231
BstAPI GCANNNNNTGC 1 cut(s) 1067
BstC8I GCNNGC 1 cut(s) 959
BstDEI CTNAG 4 cut(s) 393, 412, 540, 913
BstDSI CCRYGG 3 cut(s) 133, 660, 1490
BstEII GGTNACC 1 cut(s) 113
BstENI CCTNNNNNAGG 1 cut(s) 482
BstF5I GGATG 8 cut(s) 53, 728, 775, 882, 1185, 1255, 1308, 1500
BstHHI GCGC 2 cut(s) 731, 793
BstMAI GTCTC 1 cut(s) 740
BstMWI GCNNNNNNNGC 2 cut(s) 1067, 1291
BstNI CCWGG 1 cut(s) 272
BstPI GGTNACC 1 cut(s) 113
BstSCI CCNGG 1 cut(s) 270
BstSFI CTRYAG 3 cut(s) 425, 625, 1056
BstV1I GCAGC 5 cut(s) 163, 738, 874, 1113, 1135
BstV2I GAAGAC 1 cut(s) 1082
BstX2I RGATCY 5 cut(s) 205, 375, 441, 452, 1468
BstYI RGATCY 5 cut(s) 205, 375, 441, 452, 1468
BsuRI GGCC 2 cut(s) 459, 1099
BtgI CCRYGG 3 cut(s) 133, 660, 1490
BtsCI GGATG 8 cut(s) 53, 728, 775, 882, 1185, 1255, 1308, 1500
BtsI GCAGTG 1 cut(s) 552
BtsIMutI CAGTG 2 cut(s) 285, 552
BveI ACCTGC 3 cut(s) 722, 1052, 1275
Cac8I GCNNGC 1 cut(s) 959
CaiI CAGNNNCTG 1 cut(s) 488
CfoI GCGC 2 cut(s) 731, 793
Cfr13I GGNCC 3 cut(s) 927, 1045, 1097
Csp6I GTAC 5 cut(s) 142, 319, 405, 491, 829
CspCI CAANNNNNGTGG 2 cut(s) 694, 729
CviAII CATG 6 cut(s) 134, 661, 805, 1226, 1401, 1491
CviQI GTAC 5 cut(s) 142, 319, 405, 491, 829
DdeI CTNAG 4 cut(s) 393, 412, 540, 913
Eam1104I CTCTTC 2 cut(s) 1166, 1231
EarI CTCTTC 2 cut(s) 1166, 1231
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 6 cut(s) 133, 460, 597, 660, 1490, 1529
Eco24I GRGCYC 1 cut(s) 1490
Eco31I GGTCTC 1 cut(s) 740
Eco47I GGWCC 2 cut(s) 927, 1045
Eco57I CTGAAG 1 cut(s) 752
Eco91I GGTNACC 1 cut(s) 113
EcoNI CCTNNNNNAGG 1 cut(s) 482
EcoO109I RGGNCCY 2 cut(s) 1045, 1097
EcoO65I GGTNACC 1 cut(s) 113
EcoRII CCWGG 1 cut(s) 270
EcoT14I CCWWGG 6 cut(s) 133, 460, 597, 660, 1490, 1529
EcoT38I GRGCYC 1 cut(s) 1490
ErhI CCWWGG 6 cut(s) 133, 460, 597, 660, 1490, 1529
FaeI CATG 6 cut(s) 137, 664, 808, 1229, 1404, 1494
FatI CATG 6 cut(s) 133, 660, 804, 1225, 1400, 1490
FauI CCCGC 1 cut(s) 311
FauNDI CATATG 1 cut(s) 1287
FbaI TGATCA 1 cut(s) 1507
FblI GTMKAC 1 cut(s) 291
Fnu4HI GCNGC 5 cut(s) 177, 727, 888, 1124, 1127
FokI GGATG 8 cut(s) 40, 735, 762, 889, 1172, 1262, 1315, 1507
FriOI GRGCYC 1 cut(s) 1490
Fsp4HI GCNGC 5 cut(s) 177, 727, 888, 1124, 1127
GlaI GCGC 2 cut(s) 730, 792
GluI GCNGC 5 cut(s) 177, 727, 888, 1124, 1127
GsaI CCCAGC 1 cut(s) 1020
GsuI CTGGAG 1 cut(s) 1251
HaeIII GGCC 2 cut(s) 459, 1099
HapII CCGG 1 cut(s) 456
HhaI GCGC 2 cut(s) 731, 793
Hin1II CATG 6 cut(s) 137, 664, 808, 1229, 1404, 1494
Hin6I GCGC 2 cut(s) 729, 791
HinP1I GCGC 2 cut(s) 729, 791
HincII GTYRAC 3 cut(s) 328, 622, 1539
HindII GTYRAC 3 cut(s) 328, 622, 1539
HindIII AAGCTT 3 cut(s) 107, 761, 851
HinfI GANTC 3 cut(s) 421, 467, 1425
HpaI GTTAAC 1 cut(s) 328
HpaII CCGG 1 cut(s) 456
HphI GGTGA 5 cut(s) 121, 262, 476, 1076, 1078
Hpy166II GTNNAC 5 cut(s) 292, 319, 328, 622, 1539
Hpy188I TCNGA 7 cut(s) 63, 415, 876, 916, 1120, 1199, 1279
Hpy188III TCNNGA 6 cut(s) 433, 482, 1165, 1384, 1422, 1459
Hpy8I GTNNAC 5 cut(s) 292, 319, 328, 622, 1539
HpyAV CCTTC 4 cut(s) 432, 488, 918, 1371
HpyCH4III ACNGT 4 cut(s) 289, 390, 429, 952
HpyCH4V TGCA 7 cut(s) 366, 627, 726, 775, 1221, 1270, 1475
HpyF10VI GCNNNNNNNGC 2 cut(s) 1067, 1291
HpyF3I CTNAG 4 cut(s) 393, 412, 540, 913
Hsp92II CATG 6 cut(s) 137, 664, 808, 1229, 1404, 1494
HspAI GCGC 2 cut(s) 729, 791
Ksp22I TGATCA 1 cut(s) 1507
KspAI GTTAAC 1 cut(s) 328
LguI GCTCTTC 1 cut(s) 1231
LmnI GCTCC 5 cut(s) 7, 204, 223, 884, 1291
Lsp1109I GCAGC 5 cut(s) 163, 738, 874, 1113, 1135
LweI GCATC 6 cut(s) 235, 713, 784, 1003, 1057, 1279
MaeIII GTNAC 4 cut(s) 113, 338, 994, 1030
MfeI CAATTG 1 cut(s) 744
MflI RGATCY 5 cut(s) 205, 375, 441, 452, 1468
MhlI GDGCHC 1 cut(s) 1490
MluCI AATT 4 cut(s) 744, 862, 1253, 1503
MmeI TCCRAC 1 cut(s) 665
MroXI GAANNNNTTC 1 cut(s) 1160
MseI TTAA 2 cut(s) 90, 327
MslI CAYNNNNRTG 1 cut(s) 966
MspA1I CMGCKG 1 cut(s) 1126
MspI CCGG 1 cut(s) 456
MspR9I CCNGG 1 cut(s) 272
MunI CAATTG 1 cut(s) 744
Mva1269I GAATGC 1 cut(s) 1221
MvaI CCWGG 1 cut(s) 272
MwoI GCNNNNNNNGC 2 cut(s) 1067, 1291
NcoI CCATGG 3 cut(s) 133, 660, 1490
NdeI CATATG 1 cut(s) 1287
NlaIII CATG 6 cut(s) 137, 664, 808, 1229, 1404, 1494
NlaIV GGNNCC 6 cut(s) 9, 65, 443, 559, 1098, 1293
OliI CACNNNNGTG 1 cut(s) 966
PaqCI CACCTGC 1 cut(s) 1052
PciSI GCTCTTC 1 cut(s) 1231
PctI GAATGC 1 cut(s) 1221
PdmI GAANNNNTTC 1 cut(s) 1160
PfeI GAWTC 3 cut(s) 421, 467, 1425
PkrI GCNGC 5 cut(s) 178, 728, 889, 1125, 1128
PpuMI RGGWCCY 1 cut(s) 1045
Psp5II RGGWCCY 1 cut(s) 1045
Psp6I CCWGG 1 cut(s) 270
PspEI GGTNACC 1 cut(s) 113
PspFI CCCAGC 1 cut(s) 1016
PspGI CCWGG 1 cut(s) 270
PspN4I GGNNCC 6 cut(s) 9, 65, 443, 559, 1098, 1293
PspPI GGNCC 3 cut(s) 927, 1045, 1097
PspPPI RGGWCCY 1 cut(s) 1045
PstI CTGCAG 1 cut(s) 629
PstNI CAGNNNCTG 1 cut(s) 488
PsuI RGATCY 5 cut(s) 205, 375, 441, 452, 1468
PvuII CAGCTG 1 cut(s) 1126
RsaI GTAC 5 cut(s) 143, 320, 406, 492, 830
RsaNI GTAC 5 cut(s) 142, 319, 405, 491, 829
RseI CAYNNNNRTG 1 cut(s) 966
SapI GCTCTTC 1 cut(s) 1231
SaqAI TTAA 2 cut(s) 90, 327
SatI GCNGC 5 cut(s) 177, 727, 888, 1124, 1127
Sau96I GGNCC 3 cut(s) 927, 1045, 1097
ScrFI CCNGG 1 cut(s) 272
SduI GDGCHC 1 cut(s) 1490
SfaNI GCATC 6 cut(s) 235, 713, 784, 1003, 1057, 1279
SfcI CTRYAG 3 cut(s) 425, 625, 1056
SinI GGWCC 2 cut(s) 927, 1045
SmiMI CAYNNNNRTG 1 cut(s) 966
SmlI CTYRAG 3 cut(s) 446, 1330, 1457
SmoI CTYRAG 3 cut(s) 446, 1330, 1457
Sse9I AATT 4 cut(s) 744, 862, 1253, 1503
SsiI CCGC 3 cut(s) 5, 304, 955
SspI AATATT 1 cut(s) 612
StyD4I CCNGG 1 cut(s) 270
StyI CCWWGG 6 cut(s) 133, 460, 597, 660, 1490, 1529
TaaI ACNGT 4 cut(s) 289, 390, 429, 952
TaqI TCGA 4 cut(s) 263, 358, 685, 1521
TasI AATT 4 cut(s) 744, 862, 1253, 1503
TatI WGTACW 1 cut(s) 490
TfiI GAWTC 3 cut(s) 421, 467, 1425
Tru1I TTAA 2 cut(s) 90, 327
Tru9I TTAA 2 cut(s) 90, 327
TscAI CASTG 2 cut(s) 292, 552
TseI GCWGC 5 cut(s) 176, 726, 887, 1123, 1126
TspDTI ATGAA 4 cut(s) 533, 1184, 1214, 1266
TspGWI ACGGA 1 cut(s) 501
TspRI CASTG 2 cut(s) 292, 552
VpaK11BI GGWCC 2 cut(s) 927, 1045
XagI CCTNNNNNAGG 1 cut(s) 482
XcmI CCANNNNNNNNNTGG 1 cut(s) 937
XmiI GTMKAC 1 cut(s) 291
XmnI GAANNNNTTC 1 cut(s) 1160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.