RLG00000016035

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003748
Physical Location & Seq
Reverse (-)
3345513 .. 3347060
1548 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016035

Sequence Viewer

Length: 1377 bp
ATGGCAGAGCCAAAACCAGATACACCCTTGATCCCACCATCAGCTACGCCCTCATCCAATCGGAACCTCCCCGACTTCAAAAAATCCATTAAGCTAAAATATGTGAAGCTTGGTTACCATATTCTCATCACCCATGGAATGTACCTCTTTCTTTCCCCTCTCGTGGTTGTTATTGCTGCCCAACTCTCAACATTTTCACTCCGAGATCTTTATGATCTTTGGGAGCATCTCCAATACAACCTGATTTCGGTGATTATCTGCTCGACCCTCCTGGTGTTCCTATCCACTGTCTACTTTCTTACCCGCCCTCGCCCTGTGTACCTTGTTAACTTCTCCTGTTACAAGCCAGAAGAAACTCGAAAATGCACCAAAAAGATCTTTATGGAACAGTCTAAGATGACTGGTACATTCTCAGAGGAGAATCTACAGTTCCAGAGAAGGATCCTTGAGAGATCCGGCCTTGGTGATTCAACATACCTTCCAGAGGCTGTACTCAACATTCCTCCAAATCCGTCAATGAAAGAAGCCAGAAAAGAAGCTGAGGCAGTGATGTTTGGTGCCATTGACGAACTTTTTGCCAAGACATCTGTAAAACCCAAGGACATTGGAATATTGATTGTCAACTGCAGTTTGTTCAATCCAACTCCATCTCTTTCTGCCATGGTTATCAACCATTACCAGCTTCGAGGGAACATAGTCAGCTACAATCTTGGTGGAATGGGATGCAGCGCAGGTCTCATCTCAATTGATCTTGCTCAAAAGCTTCTTCAGGTGCATCCTAACTCTTATGCGCTGGGTGCTGATGACAAGTGCTTTAGCTGTGTTACCCAAGAAGAAGATGATGCTGGGAAGATTGGTGTTACTCTATCAAAGGACCTGATGGCTGTAGCAGGTGATGCTTTGAAGACCAATATCACGACATTGGGGCCTCTTGTTCTTCCAATGTCCGAGCAGCTGCTTTTCTTTGCTACATTGGTGGGTAGGAAACTCTTCAAGATGAAGATCAAACCTTACATCCCAGATTTCAGACTTGCTTTTGAGCATTTCTGCATTCATGCTGGAGGAAGAGCCGTCTTGGATGAATTGGAGAAGAACCTGCATCTATCTGATTGGCATATGGAGCCATCAAGGATGACACTATACCGATTTGGAAACACCTCAAGCAGTTCTCTCTGGTATGAGTTGGCTTACACAGAAGCAAAGGGAAGGATCAAGAAAGGAGATAGAACATGGCAGATAGCTTTTGGGTCGGGATTCAAGTGTAATAGTGCTGTGTGGAAAGCTCTCAAGAGCATAGATCCTGCAAAGGAAAAGAGCCCTTGGATGGCTGAAATTGATCAGTTCCCAGTCGATGTTCCAAGGGTGTCAACCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

459

Amino Acids

51.41

Weight (kDa)

8.76

Isoelectric Point (pI)

37.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 98 - 265 7.9e-77 FAE1/Type III polyketide synthase-like protein
FAE1_CUT1_RppA PF08392 266 - 330 1.2e-24 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 347 - 427 6.6e-10 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 349 - 425 1.3e-08 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 881
Acc36I ACCTGC 3 cut(s) 722, 881, 1104
AccB1I GGYRCC 1 cut(s) 557
AccI GTMKAC 1 cut(s) 291
AciI CCGC 1 cut(s) 304
AclWI GGATC 6 cut(s) 25, 436, 447, 449, 1217, 1292
AcuI CTGAAG 1 cut(s) 752
AfaI GTAC 4 cut(s) 143, 320, 406, 492
AfiI CCNNNNNNNGG 4 cut(s) 163, 247, 484, 1324
AgsI TTSAA 6 cut(s) 79, 471, 637, 904, 994, 1258
AjnI CCWGG 1 cut(s) 270
Alw26I GTCTC 1 cut(s) 740
AlwI GGATC 6 cut(s) 25, 436, 447, 449, 1217, 1292
AlwNI CAGNNNCTG 1 cut(s) 488
AoxI GGCC 2 cut(s) 457, 926
ApeKI GCWGC 4 cut(s) 176, 726, 952, 955
ArsI GACNNNNNNTTYG 2 cut(s) 557, 589
Asp700I GAANNNNTTC 1 cut(s) 989
AspLEI GCGC 2 cut(s) 731, 793
AspS9I GGNCC 2 cut(s) 874, 926
AsuHPI GGTGA 4 cut(s) 121, 262, 476, 905
AvaII GGWCC 1 cut(s) 874
BamHI GGATCC 1 cut(s) 441
BanI GGYRCC 1 cut(s) 557
BanII GRGCYC 1 cut(s) 1319
BarI GAAGNNNNNNTAC 2 cut(s) 98, 130
BauI CACGAG 1 cut(s) 161
BbsI GAAGAC 1 cut(s) 911
BbvCI CCTCAGC 1 cut(s) 540
BbvI GCAGC 4 cut(s) 163, 738, 942, 964
BccI CCATC 5 cut(s) 46, 655, 874, 1132, 1318
BceAI ACGGC 1 cut(s) 1055
BcgI CGANNNNNNTGC 2 cut(s) 557, 591
BciT130I CCWGG 1 cut(s) 272
BclI TGATCA 1 cut(s) 1336
BcoDI GTCTC 1 cut(s) 740
BfmI CTRYAG 3 cut(s) 425, 625, 885
BfuAI ACCTGC 3 cut(s) 722, 881, 1104
BglII AGATCT 2 cut(s) 205, 375
BisI GCNGC 4 cut(s) 177, 727, 953, 956
BlsI GCNGC 4 cut(s) 178, 728, 954, 957
Bme1390I CCNGG 1 cut(s) 272
Bme18I GGWCC 1 cut(s) 874
BmgT120I GGNCC 2 cut(s) 874, 926
BmiI GGNNCC 5 cut(s) 65, 443, 559, 927, 1122
BmrFI CCNGG 1 cut(s) 272
BmrI ACTGGG 1 cut(s) 1340
BmsI GCATC 6 cut(s) 235, 713, 784, 832, 886, 1108
BmuI ACTGGG 1 cut(s) 1340
BpiI GAAGAC 1 cut(s) 911
BpmI CTGGAG 1 cut(s) 1080
Bpu10I CCTNAGC 1 cut(s) 540
BpuEI CTTGAG 3 cut(s) 467, 1144, 1271
BsaBI GATNNNNATC 1 cut(s) 1001
BsaI GGTCTC 1 cut(s) 740
BsaJI CCNNGG 6 cut(s) 133, 460, 597, 660, 1319, 1358
Bsc4I CCNNNNNNNGG 4 cut(s) 163, 247, 484, 1324
Bse1I ACTGG 2 cut(s) 406, 1346
Bse8I GATNNNNATC 1 cut(s) 1001
BseBI CCWGG 1 cut(s) 272
BseDI CCNNGG 6 cut(s) 133, 460, 597, 660, 1319, 1358
BseGI GGATG 7 cut(s) 53, 728, 775, 1014, 1084, 1137, 1329
BseJI GATNNNNATC 1 cut(s) 1001
BseLI CCNNNNNNNGG 4 cut(s) 163, 247, 484, 1324
BseMII CTCAG 2 cut(s) 426, 531
BseNI ACTGG 2 cut(s) 406, 1346
BseRI GAGGAG 1 cut(s) 431
BseXI GCAGC 4 cut(s) 163, 738, 942, 964
BseYI CCCAGC 2 cut(s) 793, 845
BshFI GGCC 2 cut(s) 459, 928
BshNI GGYRCC 1 cut(s) 557
BsiSI CCGG 1 cut(s) 456
BslI CCNNNNNNNGG 4 cut(s) 163, 247, 484, 1324
BsmAI GTCTC 1 cut(s) 740
BsmI GAATGC 1 cut(s) 1050
BsnI GGCC 2 cut(s) 459, 928
Bso31I GGTCTC 1 cut(s) 740
Bsp1286I GDGCHC 1 cut(s) 1319
Bsp19I CCATGG 2 cut(s) 133, 660
BspACI CCGC 1 cut(s) 304
BspANI GGCC 2 cut(s) 459, 928
BspCNI CTCAG 2 cut(s) 425, 532
BspLI GGNNCC 5 cut(s) 65, 443, 559, 927, 1122
BspMAI CTGCAG 1 cut(s) 629
BspMI ACCTGC 3 cut(s) 722, 881, 1104
BspPI GGATC 6 cut(s) 25, 436, 447, 449, 1217, 1292
BspQI GCTCTTC 1 cut(s) 1060
BspT107I GGYRCC 1 cut(s) 557
BspTNI GGTCTC 1 cut(s) 740
BsrI ACTGG 2 cut(s) 406, 1346
BssECI CCNNGG 6 cut(s) 133, 460, 597, 660, 1319, 1358
BssSI CACGAG 1 cut(s) 161
BssT1I CCWWGG 6 cut(s) 133, 460, 597, 660, 1319, 1358
Bst2BI CACGAG 1 cut(s) 161
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 3 cut(s) 289, 390, 429
Bst6I CTCTTC 2 cut(s) 995, 1060
BstAPI GCANNNNNTGC 1 cut(s) 896
BstDEI CTNAG 3 cut(s) 393, 412, 540
BstDSI CCRYGG 2 cut(s) 133, 660
BstEII GGTNACC 1 cut(s) 113
BstENI CCTNNNNNAGG 1 cut(s) 482
BstF5I GGATG 7 cut(s) 53, 728, 775, 1014, 1084, 1137, 1329
BstHHI GCGC 2 cut(s) 731, 793
BstMAI GTCTC 1 cut(s) 740
BstMWI GCNNNNNNNGC 3 cut(s) 797, 896, 1120
BstNI CCWGG 1 cut(s) 272
BstPI GGTNACC 1 cut(s) 113
BstSCI CCNGG 1 cut(s) 270
BstSFI CTRYAG 3 cut(s) 425, 625, 885
BstV1I GCAGC 4 cut(s) 163, 738, 942, 964
BstV2I GAAGAC 1 cut(s) 911
BstX2I RGATCY 5 cut(s) 205, 375, 441, 452, 1297
BstYI RGATCY 5 cut(s) 205, 375, 441, 452, 1297
BsuRI GGCC 2 cut(s) 459, 928
BtgI CCRYGG 2 cut(s) 133, 660
BtsCI GGATG 7 cut(s) 53, 728, 775, 1014, 1084, 1137, 1329
BtsI GCAGTG 1 cut(s) 552
BtsIMutI CAGTG 2 cut(s) 285, 552
BveI ACCTGC 3 cut(s) 722, 881, 1104
CaiI CAGNNNCTG 1 cut(s) 488
CfoI GCGC 2 cut(s) 731, 793
Cfr13I GGNCC 2 cut(s) 874, 926
Csp6I GTAC 4 cut(s) 142, 319, 405, 491
CspCI CAANNNNNGTGG 2 cut(s) 694, 729
CviAII CATG 4 cut(s) 134, 661, 1055, 1230
CviQI GTAC 4 cut(s) 142, 319, 405, 491
DdeI CTNAG 3 cut(s) 393, 412, 540
Eam1104I CTCTTC 2 cut(s) 995, 1060
EarI CTCTTC 2 cut(s) 995, 1060
Eco130I CCWWGG 6 cut(s) 133, 460, 597, 660, 1319, 1358
Eco24I GRGCYC 1 cut(s) 1319
Eco31I GGTCTC 1 cut(s) 740
Eco47I GGWCC 1 cut(s) 874
Eco57I CTGAAG 1 cut(s) 752
Eco91I GGTNACC 1 cut(s) 113
EcoNI CCTNNNNNAGG 1 cut(s) 482
EcoO109I RGGNCCY 2 cut(s) 874, 926
EcoO65I GGTNACC 1 cut(s) 113
EcoRII CCWGG 1 cut(s) 270
EcoT14I CCWWGG 6 cut(s) 133, 460, 597, 660, 1319, 1358
EcoT38I GRGCYC 1 cut(s) 1319
ErhI CCWWGG 6 cut(s) 133, 460, 597, 660, 1319, 1358
FaeI CATG 4 cut(s) 137, 664, 1058, 1233
FatI CATG 4 cut(s) 133, 660, 1054, 1229
FauI CCCGC 1 cut(s) 311
FauNDI CATATG 1 cut(s) 1116
FbaI TGATCA 1 cut(s) 1336
FblI GTMKAC 1 cut(s) 291
Fnu4HI GCNGC 4 cut(s) 177, 727, 953, 956
FokI GGATG 7 cut(s) 40, 735, 762, 1001, 1091, 1144, 1336
FriOI GRGCYC 1 cut(s) 1319
Fsp4HI GCNGC 4 cut(s) 177, 727, 953, 956
GlaI GCGC 2 cut(s) 730, 792
GluI GCNGC 4 cut(s) 177, 727, 953, 956
GsaI CCCAGC 2 cut(s) 797, 849
GsuI CTGGAG 1 cut(s) 1080
HaeIII GGCC 2 cut(s) 459, 928
HapII CCGG 1 cut(s) 456
HhaI GCGC 2 cut(s) 731, 793
Hin1II CATG 4 cut(s) 137, 664, 1058, 1233
Hin6I GCGC 2 cut(s) 729, 791
HinP1I GCGC 2 cut(s) 729, 791
HincII GTYRAC 3 cut(s) 328, 622, 1368
HindII GTYRAC 3 cut(s) 328, 622, 1368
HindIII AAGCTT 2 cut(s) 107, 761
HinfI GANTC 3 cut(s) 421, 467, 1254
HpaI GTTAAC 1 cut(s) 328
HpaII CCGG 1 cut(s) 456
HphI GGTGA 4 cut(s) 121, 262, 476, 905
Hpy166II GTNNAC 5 cut(s) 292, 319, 328, 622, 1368
Hpy188I TCNGA 6 cut(s) 63, 203, 415, 949, 1028, 1108
Hpy188III TCNNGA 7 cut(s) 433, 482, 916, 994, 1213, 1251, 1288
Hpy8I GTNNAC 5 cut(s) 292, 319, 328, 622, 1368
HpyAV CCTTC 3 cut(s) 432, 488, 1200
HpyCH4III ACNGT 3 cut(s) 289, 390, 429
HpyCH4V TGCA 7 cut(s) 366, 627, 726, 775, 1050, 1099, 1304
HpyF10VI GCNNNNNNNGC 3 cut(s) 797, 896, 1120
HpyF3I CTNAG 3 cut(s) 393, 412, 540
Hsp92II CATG 4 cut(s) 137, 664, 1058, 1233
HspAI GCGC 2 cut(s) 729, 791
Ksp22I TGATCA 1 cut(s) 1336
KspAI GTTAAC 1 cut(s) 328
LguI GCTCTTC 1 cut(s) 1060
LmnI GCTCC 2 cut(s) 223, 1120
Lsp1109I GCAGC 4 cut(s) 163, 738, 942, 964
LweI GCATC 6 cut(s) 235, 713, 784, 832, 886, 1108
MaeIII GTNAC 4 cut(s) 113, 338, 823, 859
MfeI CAATTG 1 cut(s) 744
MflI RGATCY 5 cut(s) 205, 375, 441, 452, 1297
MhlI GDGCHC 1 cut(s) 1319
MluCI AATT 3 cut(s) 744, 1082, 1332
MmeI TCCRAC 1 cut(s) 665
MroXI GAANNNNTTC 1 cut(s) 989
MseI TTAA 2 cut(s) 90, 327
MspA1I CMGCKG 1 cut(s) 955
MspI CCGG 1 cut(s) 456
MspR9I CCNGG 1 cut(s) 272
MunI CAATTG 1 cut(s) 744
Mva1269I GAATGC 1 cut(s) 1050
MvaI CCWGG 1 cut(s) 272
MwoI GCNNNNNNNGC 3 cut(s) 797, 896, 1120
NcoI CCATGG 2 cut(s) 133, 660
NdeI CATATG 1 cut(s) 1116
NlaIII CATG 4 cut(s) 137, 664, 1058, 1233
NlaIV GGNNCC 5 cut(s) 65, 443, 559, 927, 1122
PaqCI CACCTGC 1 cut(s) 881
PciSI GCTCTTC 1 cut(s) 1060
PctI GAATGC 1 cut(s) 1050
PdmI GAANNNNTTC 1 cut(s) 989
PfeI GAWTC 3 cut(s) 421, 467, 1254
PkrI GCNGC 4 cut(s) 178, 728, 954, 957
PpuMI RGGWCCY 1 cut(s) 874
Psp5II RGGWCCY 1 cut(s) 874
Psp6I CCWGG 1 cut(s) 270
PspEI GGTNACC 1 cut(s) 113
PspFI CCCAGC 2 cut(s) 793, 845
PspGI CCWGG 1 cut(s) 270
PspN4I GGNNCC 5 cut(s) 65, 443, 559, 927, 1122
PspPI GGNCC 2 cut(s) 874, 926
PspPPI RGGWCCY 1 cut(s) 874
PstI CTGCAG 1 cut(s) 629
PstNI CAGNNNCTG 1 cut(s) 488
PsuI RGATCY 5 cut(s) 205, 375, 441, 452, 1297
PvuII CAGCTG 1 cut(s) 955
RsaI GTAC 4 cut(s) 143, 320, 406, 492
RsaNI GTAC 4 cut(s) 142, 319, 405, 491
SapI GCTCTTC 1 cut(s) 1060
SaqAI TTAA 2 cut(s) 90, 327
SatI GCNGC 4 cut(s) 177, 727, 953, 956
Sau96I GGNCC 2 cut(s) 874, 926
ScrFI CCNGG 1 cut(s) 272
SduI GDGCHC 1 cut(s) 1319
SfaNI GCATC 6 cut(s) 235, 713, 784, 832, 886, 1108
SfcI CTRYAG 3 cut(s) 425, 625, 885
SinI GGWCC 1 cut(s) 874
SmlI CTYRAG 3 cut(s) 446, 1159, 1286
SmoI CTYRAG 3 cut(s) 446, 1159, 1286
Sse9I AATT 3 cut(s) 744, 1082, 1332
SsiI CCGC 1 cut(s) 304
SspI AATATT 1 cut(s) 612
StyD4I CCNGG 1 cut(s) 270
StyI CCWWGG 6 cut(s) 133, 460, 597, 660, 1319, 1358
TaaI ACNGT 3 cut(s) 289, 390, 429
TaqI TCGA 4 cut(s) 263, 358, 685, 1350
TasI AATT 3 cut(s) 744, 1082, 1332
TatI WGTACW 1 cut(s) 490
TfiI GAWTC 3 cut(s) 421, 467, 1254
Tru1I TTAA 2 cut(s) 90, 327
Tru9I TTAA 2 cut(s) 90, 327
TscAI CASTG 2 cut(s) 292, 552
TseI GCWGC 4 cut(s) 176, 726, 952, 955
TspDTI ATGAA 4 cut(s) 533, 1013, 1043, 1095
TspGWI ACGGA 1 cut(s) 501
TspRI CASTG 2 cut(s) 292, 552
VpaK11BI GGWCC 1 cut(s) 874
XagI CCTNNNNNAGG 1 cut(s) 482
XmiI GTMKAC 1 cut(s) 291
XmnI GAANNNNTTC 1 cut(s) 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.