pycom15g10700

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003748
Physical Location & Seq
Forward (+)
7158327 .. 7159214
888 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g10700.1

Sequence Viewer

Length: 888 bp
ATGATTATCAATCATTACAGGCTGCGAGGGAATATTATTAGTTATAACCTTGGTGGGATGGGATGTAGTGCCGGTTTGATCTCAATTGATCTTGCTAAAGATCTTCTTCGAGTCCACCCGAATACCTATGCTTTGGTCATTAGCATGGAAAACATCACATTGAATTGGTATTTCGGGAATGATAGATCGATGCTTGTCTCAAACTGCTTGTTTAGAATGGGAGGAGCTGCAATTTTGCTTTCGAACAAATGGTCAGACAGACGTCGATCCAAGTATCAGTTGGTGCACACTGTTCGGACCCACAAAGGTTCGGATGACAAGTGCTTCTCTTGTGTTACCCAACAAGAGGATTTCGCGGGGAAAATTGGAGTTTCTTTGTCAAAGGATCTCATGGCGGTTGCTGGGGATGCTTTAAAGACCAACATCACTACCTTGGGTCCTCTTGTGTTACCTATGTCCGAACAGTTGCTCTTCTTTGCCACGCTTGTTGGGAGAAAACTTTTGAAGATGAAAGTGAAGCCTTACATCCCGGATTTCAAGTTGGCTTTTGAGCATTTCTGCATTCATGCCGGAGGAAGAGCTGTGCTAGATGAATTGGAGAAAAACTTGCAGCTTTCGGACCACCACATGGAGCCTTCGAGAATGACACTTCACAGATTTGGCAACACTTCAAGTAGCTCTCTTTGGTACGAACTTGCTTATACAGAAGCCAAGGGGAGGATGAAGAAAGGAGACCGAACTTGGCAGATAGCATTCGGGTCCGGGTTCAAGTGCAACAGTGCAGTTTGGAAGGCTTTGAGAACTGTGAATCCAGCAGAGGAGAAGAATCCATGGATGGATGAGATCCACCAGTTCCCGGTGGATGTTCCAAAGGTCTCAGCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

296

Amino Acids

33.23

Weight (kDa)

9.31

Isoelectric Point (pI)

35.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 1 - 167 2.8e-87 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 184 - 264 4.2e-11 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 186 - 262 4.4e-10 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 45
AatII GACGTC 1 cut(s) 265
AccB7I CCANNNNNTGG 1 cut(s) 628
AccII CGCG 1 cut(s) 356
AciI CCGC 2 cut(s) 356, 395
AclWI GGATC 3 cut(s) 261, 393, 838
AcyI GRCGYC 1 cut(s) 262
AfaI GTAC 1 cut(s) 689
AfiI CCNNNNNNNGG 4 cut(s) 346, 628, 717, 856
AgsI TTSAA 5 cut(s) 163, 505, 538, 672, 769
AloI GAACNNNNNNTCC 2 cut(s) 793, 825
AluBI AGCT 5 cut(s) 227, 581, 613, 678, 881
AluI AGCT 5 cut(s) 227, 581, 613, 678, 881
Alw21I GWGCWC 1 cut(s) 288
Alw26I GTCTC 3 cut(s) 202, 726, 880
Alw44I GTGCAC 1 cut(s) 284
AlwI GGATC 3 cut(s) 261, 393, 838
ApaLI GTGCAC 1 cut(s) 284
ApeKI GCWGC 3 cut(s) 22, 227, 610
ArsI GACNNNNNNTTYG 2 cut(s) 236, 268
AspS9I GGNCC 4 cut(s) 297, 437, 619, 759
AsuC2I CCSGG 3 cut(s) 530, 763, 857
AsuII TTCGAA 1 cut(s) 242
AvaII GGWCC 4 cut(s) 297, 437, 619, 759
BaeGI GKGCMC 1 cut(s) 288
Bbv12I GWGCWC 1 cut(s) 288
BbvI GCAGC 3 cut(s) 9, 214, 622
BccI CCATC 2 cut(s) 52, 829
BcgI CGANNNNNNTGC 2 cut(s) 275, 309
BcnI CCSGG 3 cut(s) 530, 763, 857
BcoDI GTCTC 3 cut(s) 202, 726, 880
BfaI CTAG 1 cut(s) 587
BglII AGATCT 1 cut(s) 100
BisI GCNGC 3 cut(s) 23, 228, 611
BlsI GCNGC 3 cut(s) 24, 229, 612
Bme1390I CCNGG 3 cut(s) 530, 763, 857
Bme18I GGWCC 4 cut(s) 297, 437, 619, 759
BmgT120I GGNCC 4 cut(s) 297, 437, 619, 759
BmiI GGNNCC 4 cut(s) 299, 438, 633, 760
BmrFI CCNGG 3 cut(s) 530, 763, 857
BmsI GCATC 2 cut(s) 180, 397
BoxI GACNNNNGTC 1 cut(s) 261
Bpu14I TTCGAA 1 cut(s) 242
BpuMI CCSGG 3 cut(s) 530, 763, 857
Bsa29I ATCGAT 1 cut(s) 188
BsaHI GRCGYC 1 cut(s) 262
BsaI GGTCTC 2 cut(s) 726, 880
BsaJI CCNNGG 4 cut(s) 49, 432, 711, 830
Bsc4I CCNNNNNNNGG 4 cut(s) 346, 628, 717, 856
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 1 cut(s) 850
BseCI ATCGAT 1 cut(s) 188
BseDI CCNNGG 4 cut(s) 49, 432, 711, 830
BseGI GGATG 9 cut(s) 63, 68, 319, 412, 525, 726, 840, 844, 868
BseLI CCNNNNNNNGG 4 cut(s) 346, 628, 717, 856
BseNI ACTGG 1 cut(s) 850
BseRI GAGGAG 2 cut(s) 237, 833
BseSI GKGCMC 1 cut(s) 288
BseXI GCAGC 3 cut(s) 9, 214, 622
BseYI CCCAGC 1 cut(s) 401
BsgI GTGCAG 1 cut(s) 801
Bsh1236I CGCG 1 cut(s) 356
BshVI ATCGAT 1 cut(s) 188
BsiHKAI GWGCWC 1 cut(s) 288
BsiSI CCGG 5 cut(s) 72, 530, 570, 762, 857
BslI CCNNNNNNNGG 4 cut(s) 346, 628, 717, 856
BsmAI GTCTC 3 cut(s) 202, 726, 880
BsmI GAATGC 2 cut(s) 561, 752
Bso31I GGTCTC 2 cut(s) 726, 880
Bsp119I TTCGAA 1 cut(s) 242
Bsp1286I GDGCHC 1 cut(s) 288
Bsp143I GATC 7 cut(s) 78, 88, 100, 185, 266, 385, 843
Bsp19I CCATGG 1 cut(s) 830
BspACI CCGC 2 cut(s) 356, 395
BspDI ATCGAT 1 cut(s) 188
BspFNI CGCG 1 cut(s) 356
BspLI GGNNCC 4 cut(s) 299, 438, 633, 760
BspPI GGATC 3 cut(s) 261, 393, 838
BspQI GCTCTTC 2 cut(s) 476, 571
BspT104I TTCGAA 1 cut(s) 242
BspTNI GGTCTC 2 cut(s) 726, 880
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 1 cut(s) 850
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 4 cut(s) 49, 432, 711, 830
BssMI GATC 7 cut(s) 78, 88, 100, 185, 266, 385, 843
BssNI GRCGYC 1 cut(s) 262
BssT1I CCWWGG 4 cut(s) 49, 432, 711, 830
Bst4CI ACNGT 4 cut(s) 292, 465, 779, 805
Bst6I CTCTTC 2 cut(s) 476, 571
BstACI GRCGYC 1 cut(s) 262
BstBI TTCGAA 1 cut(s) 242
BstDEI CTNAG 1 cut(s) 877
BstDSI CCRYGG 1 cut(s) 830
BstF5I GGATG 9 cut(s) 63, 68, 319, 412, 525, 726, 840, 844, 868
BstFNI CGCG 1 cut(s) 356
BstKTI GATC 7 cut(s) 81, 91, 103, 188, 269, 388, 846
BstMAI GTCTC 3 cut(s) 202, 726, 880
BstMBI GATC 7 cut(s) 78, 88, 100, 185, 266, 385, 843
BstMWI GCNNNNNNNGC 1 cut(s) 407
BstPAI GACNNNNGTC 1 cut(s) 261
BstSCI CCNGG 3 cut(s) 528, 761, 855
BstSLI GKGCMC 1 cut(s) 288
BstUI CGCG 1 cut(s) 356
BstV1I GCAGC 3 cut(s) 9, 214, 622
BstX2I RGATCY 3 cut(s) 100, 385, 843
BstYI RGATCY 3 cut(s) 100, 385, 843
Bsu15I ATCGAT 1 cut(s) 188
BsuTUI ATCGAT 1 cut(s) 188
BtgI CCRYGG 1 cut(s) 830
BtsCI GGATG 9 cut(s) 63, 68, 319, 412, 525, 726, 840, 844, 868
BtsIMutI CAGTG 2 cut(s) 288, 784
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 4 cut(s) 297, 437, 619, 759
ClaI ATCGAT 1 cut(s) 188
Csp6I GTAC 1 cut(s) 688
CspCI CAANNNNNGTGG 2 cut(s) 469, 504
CviAII CATG 5 cut(s) 145, 391, 566, 628, 831
CviQI GTAC 1 cut(s) 688
DdeI CTNAG 1 cut(s) 877
DpnI GATC 7 cut(s) 80, 90, 102, 187, 268, 387, 845
DpnII GATC 7 cut(s) 78, 88, 100, 185, 266, 385, 843
DraI TTTAAA 1 cut(s) 414
Eam1104I CTCTTC 2 cut(s) 476, 571
EarI CTCTTC 2 cut(s) 476, 571
Eco130I CCWWGG 4 cut(s) 49, 432, 711, 830
Eco31I GGTCTC 2 cut(s) 726, 880
Eco47I GGWCC 4 cut(s) 297, 437, 619, 759
EcoO109I RGGNCCY 1 cut(s) 437
EcoT14I CCWWGG 4 cut(s) 49, 432, 711, 830
ErhI CCWWGG 4 cut(s) 49, 432, 711, 830
FaeI CATG 5 cut(s) 148, 394, 569, 631, 834
FaiI YATR 9 cut(s) 45, 129, 146, 392, 455, 567, 629, 702, 832
FalI AAGNNNNNCTT 2 cut(s) 90, 122
FatI CATG 5 cut(s) 144, 390, 565, 627, 830
FauI CCCGC 1 cut(s) 349
Fnu4HI GCNGC 3 cut(s) 23, 228, 611
FokI GGATG 9 cut(s) 70, 75, 326, 419, 512, 733, 847, 851, 875
Fsp4HI GCNGC 3 cut(s) 23, 228, 611
FspBI CTAG 1 cut(s) 587
GluI GCNGC 3 cut(s) 23, 228, 611
GsaI CCCAGC 1 cut(s) 405
HapII CCGG 5 cut(s) 72, 530, 570, 762, 857
Hin1I GRCGYC 1 cut(s) 262
Hin1II CATG 5 cut(s) 148, 394, 569, 631, 834
HinfI GANTC 3 cut(s) 111, 808, 826
HpaII CCGG 5 cut(s) 72, 530, 570, 762, 857
Hpy166II GTNNAC 2 cut(s) 115, 286
Hpy188I TCNGA 6 cut(s) 256, 297, 313, 460, 619, 887
Hpy188III TCNNGA 2 cut(s) 175, 639
Hpy8I GTNNAC 2 cut(s) 115, 286
Hpy99I CGWCG 1 cut(s) 267
HpyAV CCTTC 2 cut(s) 645, 784
HpyCH4III ACNGT 4 cut(s) 292, 465, 779, 805
HpyCH4IV ACGT 1 cut(s) 262
HpyCH4V TGCA 6 cut(s) 230, 286, 561, 610, 774, 782
HpyF10VI GCNNNNNNNGC 1 cut(s) 407
HpyF3I CTNAG 1 cut(s) 877
HpySE526I ACGT 1 cut(s) 262
Hsp92I GRCGYC 1 cut(s) 262
Hsp92II CATG 5 cut(s) 148, 394, 569, 631, 834
Kzo9I GATC 7 cut(s) 78, 88, 100, 185, 266, 385, 843
LguI GCTCTTC 2 cut(s) 476, 571
LmnI GCTCC 2 cut(s) 224, 631
LpnPI CCDG 9 cut(s) 4, 85, 387, 543, 583, 775, 825, 863, 870
Lsp1109I GCAGC 3 cut(s) 9, 214, 622
LweI GCATC 2 cut(s) 180, 397
MaeI CTAG 1 cut(s) 587
MaeII ACGT 1 cut(s) 262
MaeIII GTNAC 2 cut(s) 334, 447
MalI GATC 7 cut(s) 80, 90, 102, 187, 268, 387, 845
MboI GATC 7 cut(s) 78, 88, 100, 185, 266, 385, 843
MboII GAAGA 7 cut(s) 95, 98, 463, 517, 588, 736, 835
MfeI CAATTG 1 cut(s) 84
MflI RGATCY 3 cut(s) 100, 385, 843
MhlI GDGCHC 1 cut(s) 288
MluCI AATT 5 cut(s) 84, 163, 231, 363, 593
MlyI GAGTC 1 cut(s) 120
MnlI CCTC 7 cut(s) 20, 215, 340, 450, 566, 711, 811
MseI TTAA 1 cut(s) 413
MslI CAYNNNNRTG 1 cut(s) 143
MspI CCGG 5 cut(s) 72, 530, 570, 762, 857
MspR9I CCNGG 3 cut(s) 530, 763, 857
MunI CAATTG 1 cut(s) 84
Mva1269I GAATGC 2 cut(s) 561, 752
MvnI CGCG 1 cut(s) 356
MwoI GCNNNNNNNGC 1 cut(s) 407
NciI CCSGG 3 cut(s) 530, 763, 857
NcoI CCATGG 1 cut(s) 830
NdeII GATC 7 cut(s) 78, 88, 100, 185, 266, 385, 843
NlaIII CATG 5 cut(s) 148, 394, 569, 631, 834
NlaIV GGNNCC 4 cut(s) 299, 438, 633, 760
NspV TTCGAA 1 cut(s) 242
PciSI GCTCTTC 2 cut(s) 476, 571
PctI GAATGC 2 cut(s) 561, 752
PfeI GAWTC 2 cut(s) 808, 826
PflMI CCANNNNNTGG 1 cut(s) 628
PfoI TCCNGGA 1 cut(s) 528
PkrI GCNGC 3 cut(s) 24, 229, 612
PleI GAGTC 1 cut(s) 119
PpsI GAGTC 1 cut(s) 119
PpuMI RGGWCCY 1 cut(s) 437
PshAI GACNNNNGTC 1 cut(s) 261
PsiI TTATAA 1 cut(s) 45
Psp5II RGGWCCY 1 cut(s) 437
PspFI CCCAGC 1 cut(s) 401
PspN4I GGNNCC 4 cut(s) 299, 438, 633, 760
PspPI GGNCC 4 cut(s) 297, 437, 619, 759
PspPPI RGGWCCY 1 cut(s) 437
PsuI RGATCY 3 cut(s) 100, 385, 843
RsaI GTAC 1 cut(s) 689
RsaNI GTAC 1 cut(s) 688
RseI CAYNNNNRTG 1 cut(s) 143
SapI GCTCTTC 2 cut(s) 476, 571
SaqAI TTAA 1 cut(s) 413
SatI GCNGC 3 cut(s) 23, 228, 611
Sau3AI GATC 7 cut(s) 78, 88, 100, 185, 266, 385, 843
Sau96I GGNCC 4 cut(s) 297, 437, 619, 759
SchI GAGTC 1 cut(s) 120
ScrFI CCNGG 3 cut(s) 530, 763, 857
SduI GDGCHC 1 cut(s) 288
SfaNI GCATC 2 cut(s) 180, 397
SfuI TTCGAA 1 cut(s) 242
SinI GGWCC 4 cut(s) 297, 437, 619, 759
SmiMI CAYNNNNRTG 1 cut(s) 143
Sse9I AATT 5 cut(s) 84, 163, 231, 363, 593
SsiI CCGC 2 cut(s) 356, 395
SspI AATATT 1 cut(s) 34
SspMI CTAG 1 cut(s) 587
StyD4I CCNGG 3 cut(s) 528, 761, 855
StyI CCWWGG 4 cut(s) 49, 432, 711, 830
TaaI ACNGT 4 cut(s) 292, 465, 779, 805
TaiI ACGT 1 cut(s) 265
TaqI TCGA 5 cut(s) 109, 188, 242, 265, 638
TaqII GACCGA 1 cut(s) 750
TasI AATT 5 cut(s) 84, 163, 231, 363, 593
TfiI GAWTC 2 cut(s) 808, 826
Tru1I TTAA 1 cut(s) 413
Tru9I TTAA 1 cut(s) 413
TscAI CASTG 2 cut(s) 295, 784
TseI GCWGC 3 cut(s) 22, 227, 610
TspDTI ATGAA 4 cut(s) 524, 554, 606, 737
TspRI CASTG 2 cut(s) 295, 784
Van91I CCANNNNNTGG 1 cut(s) 628
VneI GTGCAC 1 cut(s) 284
VpaK11BI GGWCC 4 cut(s) 297, 437, 619, 759
XcmI CCANNNNNNNNNTGG 1 cut(s) 277
XspI CTAG 1 cut(s) 587
ZraI GACGTC 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.