MD15G1178600.v1.1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003748
Physical Location & Seq
Reverse (-)
14058402 .. 14061325
2924 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1178600.v1.1.491

Sequence Viewer

Length: 1557 bp
ATGGCAGACCCAAAACCAGACACACCTTTGATCCCACCATCTTCGTCGTCGTCTTCATCATCCTCGTCGCGAAACCTCCCCGACTTCAAAAAATCTATCAAGCTAAAATATGTGAAGCTTGGATACCATATCCTGATCACCCATGGAATGTACCTCTTTCTTTCCCCTCTTGTTGTCGTTATTGCTGCCCAGATTTCGACATTTTCTCTCAAGGATCTCTATGATCTTTGGGAGCATCTCCAGTACAACCTGATTTCAGTGATTATCTGCTCTACTCTCCTTGTTTTCTTGTCCACTGTCTACTTTGTAACCCGACCTCGCCCGGTGTACCTTGTTAACTTTTCCTGCTACAAGCCAGAAGAATCTCGAAAGTGCACGAAAAAGATTTTCATGGAACAGTCCCAGATGACTGGTACATTTACAGAGGACAATCTTCAGTTCCAGCGAAGGATCCTTGAGAGATCCGGCCTTGGTGATTCGACTTACCTTCCAGAGGCTGTACTCAACATTCCTCCAAATCCGTCAATGAAAGAAGCTAGAAAAGAAGCCGAGGCTGTGATGTTTGGTGCCATTGATGAGCTTTTGGCTAAGACAGCTGTAAAACCTAAGGACATTGGAATTTTGATTGTGAACTGCAGCTTGTTCAATCCAACCCCATCTCTTTCTGCCATGGTTATCAACCATTACCAGCTTCGAGGGAACATAGTCAGCTACAATCTGGGTGGGATGGGATGCAGTGCAGGTCTGATCTCGATCGATCTTGCTAAAAATCTTCTTCAGGTGCATCCCAACTCTTATGCACTGGTTATCAGCATGGAGAACATCACCTTGAACTGGTACTTTGGAAATGACCGCTCAAAACTTGTCTCAAATTGCTTATTCCGTATGGGAGGGGCTGCTGTATTGCTTTCAAACAAAAGCTCAGACAAAAGAAGGTCCAAGTACCAATTGGTTCATACTGTTCGTACCCACAAGGGTGCTGATGATAAGTGCTTTAGCTGCGTTACCCAAGAAGAAGACGATAACGGGAAGATTGGTGTTACTTTATCAAAGGATCTGATGGCAGTAGCAGGTGATGCTCTAAAGACGAACATCACCACATTGGGGCCTCTTGTGCTGCCAATGTCCGAACAGCTGCTTTTCTTTGCTACATTGGTTGGGAGGAAACTGTTCAAGATGAAGATCAAACCATACATCCCAGATTTCAGACTGGCTTTTGAGCATTTCTGTATTCATGCTGGAGGAAGAGCCGTCTTGGACGAATTGGAGAAGAACCTTCATCTATCAGATTGGCATATGGAGCCATCGAGGATGACACTCTATCGATTTGGAAACACCTCAAGCAGCTCTCTATGGTATGAACTAGCTTACACAGAAGCCAAGGGAAGGATGAAGAAAGGAGATAGAACATGGCAGATAGCTTTTGGGTCGGGATTCAAGTGCAACAGTGCTGTGTGGAAGGCTCTTAGGAGCATAAACCCCGCAAAGGAAAAGAACCCGTGGATGGAAGAAATTGATCGGTTCCCAGTCGATGTTCCAAGGGTGTCAACCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

519

Amino Acids

58.26

Weight (kDa)

9.16

Isoelectric Point (pI)

41.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 101 - 390 1.5e-145 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 407 - 487 1.8e-09 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 409 - 485 9.1e-09 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1061
Acc36I ACCTGC 2 cut(s) 731, 1061
AccB1I GGYRCC 1 cut(s) 566
AccBSI CCGCTC 1 cut(s) 855
AccI GTMKAC 1 cut(s) 300
AccII CGCG 1 cut(s) 70
AciI CCGC 2 cut(s) 853, 1482
AclWI GGATC 6 cut(s) 25, 222, 445, 456, 458, 1062
AcsI RAATTY 1 cut(s) 618
AcuI CTGAAG 2 cut(s) 419, 761
AfaI GTAC 8 cut(s) 152, 245, 329, 415, 501, 839, 944, 967
AfiI CCNNNNNNNGG 6 cut(s) 493, 834, 1104, 1386, 1486, 1504
AgsI TTSAA 6 cut(s) 88, 646, 832, 912, 1174, 1438
AleI CACNNNNGTG 1 cut(s) 975
Alw21I GWGCWC 1 cut(s) 377
Alw26I GTCTC 1 cut(s) 871
Alw44I GTGCAC 1 cut(s) 373
AlwI GGATC 6 cut(s) 25, 222, 445, 456, 458, 1062
AlwNI CAGNNNCTG 1 cut(s) 497
AoxI GGCC 2 cut(s) 466, 1106
ApaLI GTGCAC 1 cut(s) 373
ApeKI GCWGC 7 cut(s) 185, 636, 896, 999, 1117, 1135, 1344
ApoI RAATTY 1 cut(s) 618
Asp700I GAANNNNTTC 1 cut(s) 1169
AspS9I GGNCC 2 cut(s) 936, 1106
AsuC2I CCSGG 1 cut(s) 323
AsuHPI GGTGA 5 cut(s) 130, 485, 817, 1085, 1087
AvaII GGWCC 1 cut(s) 936
AxyI CCTNAGG 1 cut(s) 606
BaeGI GKGCMC 1 cut(s) 377
BamHI GGATCC 1 cut(s) 450
BanI GGYRCC 1 cut(s) 566
BarI GAAGNNNNNNTAC 2 cut(s) 107, 139
BbsI GAAGAC 2 cut(s) 45, 1023
Bbv12I GWGCWC 1 cut(s) 377
BbvI GCAGC 7 cut(s) 172, 648, 883, 986, 1104, 1122, 1356
BccI CCATC 6 cut(s) 46, 664, 721, 1054, 1312, 1498
BceAI ACGGC 1 cut(s) 1235
BciVI GTATCC 1 cut(s) 116
BclI TGATCA 1 cut(s) 135
BcnI CCSGG 1 cut(s) 323
BcoDI GTCTC 1 cut(s) 871
BfaI CTAG 2 cut(s) 537, 1364
BfmI CTRYAG 1 cut(s) 634
BfuAI ACCTGC 2 cut(s) 731, 1061
BfuI GTATCC 1 cut(s) 116
BisI GCNGC 7 cut(s) 186, 637, 897, 1000, 1118, 1136, 1345
BlsI GCNGC 7 cut(s) 187, 638, 898, 1001, 1119, 1137, 1346
Bme1390I CCNGG 1 cut(s) 323
Bme18I GGWCC 1 cut(s) 936
BmgT120I GGNCC 2 cut(s) 936, 1106
BmiI GGNNCC 5 cut(s) 452, 568, 1107, 1302, 1523
BmrFI CCNGG 1 cut(s) 323
BmrI ACTGGG 1 cut(s) 1520
BmsI GCATC 4 cut(s) 244, 722, 793, 1066
BmuI ACTGGG 1 cut(s) 1520
BpiI GAAGAC 2 cut(s) 45, 1023
BpmI CTGGAG 2 cut(s) 224, 1260
BpuEI CTTGAG 3 cut(s) 194, 476, 1324
BpuMI CCSGG 1 cut(s) 323
Bsa29I ATCGAT 2 cut(s) 756, 1324
BsaBI GATNNNNATC 2 cut(s) 752, 1181
BsaJI CCNNGG 7 cut(s) 142, 469, 549, 669, 1380, 1499, 1538
Bsc4I CCNNNNNNNGG 6 cut(s) 493, 834, 1104, 1386, 1486, 1504
Bse1I ACTGG 6 cut(s) 241, 415, 807, 839, 1215, 1526
Bse21I CCTNAGG 1 cut(s) 606
Bse8I GATNNNNATC 2 cut(s) 752, 1181
BseCI ATCGAT 2 cut(s) 756, 1324
BseDI CCNNGG 7 cut(s) 142, 469, 549, 669, 1380, 1499, 1538
BseGI GGATG 8 cut(s) 59, 732, 737, 784, 1194, 1317, 1395, 1509
BseJI GATNNNNATC 2 cut(s) 752, 1181
BseLI CCNNNNNNNGG 6 cut(s) 493, 834, 1104, 1386, 1486, 1504
BseMII CTCAG 1 cut(s) 936
BseNI ACTGG 6 cut(s) 241, 415, 807, 839, 1215, 1526
BseSI GKGCMC 1 cut(s) 377
BseXI GCAGC 7 cut(s) 172, 648, 883, 986, 1104, 1122, 1356
BsgI GTGCAG 1 cut(s) 759
Bsh1236I CGCG 1 cut(s) 70
Bsh1285I CGRYCG 1 cut(s) 756
BshFI GGCC 2 cut(s) 468, 1108
BshNI GGYRCC 1 cut(s) 566
BshVI ATCGAT 2 cut(s) 756, 1324
BsiEI CGRYCG 1 cut(s) 756
BsiHKAI GWGCWC 1 cut(s) 377
BsiSI CCGG 2 cut(s) 323, 465
BslFI GGGAC 1 cut(s) 385
BslI CCNNNNNNNGG 6 cut(s) 493, 834, 1104, 1386, 1486, 1504
BsmAI GTCTC 1 cut(s) 871
BsmFI GGGAC 1 cut(s) 385
BsnI GGCC 2 cut(s) 468, 1108
Bsp1286I GDGCHC 1 cut(s) 377
Bsp19I CCATGG 2 cut(s) 142, 669
Bsp68I TCGCGA 1 cut(s) 70
BspACI CCGC 2 cut(s) 853, 1482
BspANI GGCC 2 cut(s) 468, 1108
BspCNI CTCAG 1 cut(s) 935
BspDI ATCGAT 2 cut(s) 756, 1324
BspFNI CGCG 1 cut(s) 70
BspLI GGNNCC 5 cut(s) 452, 568, 1107, 1302, 1523
BspMAI CTGCAG 1 cut(s) 638
BspMI ACCTGC 2 cut(s) 731, 1061
BspPI GGATC 6 cut(s) 25, 222, 445, 456, 458, 1062
BspQI GCTCTTC 1 cut(s) 1240
BspT107I GGYRCC 1 cut(s) 566
BsrBI CCGCTC 1 cut(s) 855
BsrI ACTGG 6 cut(s) 241, 415, 807, 839, 1215, 1526
BssECI CCNNGG 7 cut(s) 142, 469, 549, 669, 1380, 1499, 1538
BssT1I CCWWGG 5 cut(s) 142, 469, 669, 1380, 1538
Bst4CI ACNGT 5 cut(s) 298, 399, 961, 1170, 1448
Bst6I CTCTTC 1 cut(s) 1240
BstAPI GCANNNNNTGC 1 cut(s) 1076
BstDEI CTNAG 4 cut(s) 588, 606, 922, 1466
BstDSI CCRYGG 3 cut(s) 142, 669, 1499
BstENI CCTNNNNNAGG 1 cut(s) 491
BstF5I GGATG 8 cut(s) 59, 732, 737, 784, 1194, 1317, 1395, 1509
BstFNI CGCG 1 cut(s) 70
BstMAI GTCTC 1 cut(s) 871
BstMCI CGRYCG 1 cut(s) 756
BstMWI GCNNNNNNNGC 5 cut(s) 593, 999, 1076, 1114, 1300
BstSCI CCNGG 1 cut(s) 321
BstSFI CTRYAG 1 cut(s) 634
BstSLI GKGCMC 1 cut(s) 377
BstUI CGCG 1 cut(s) 70
BstV1I GCAGC 7 cut(s) 172, 648, 883, 986, 1104, 1122, 1356
BstV2I GAAGAC 2 cut(s) 45, 1023
BstX2I RGATCY 4 cut(s) 214, 450, 461, 1054
BstXI CCANNNNNNTGG 1 cut(s) 410
BstYI RGATCY 4 cut(s) 214, 450, 461, 1054
Bsu15I ATCGAT 2 cut(s) 756, 1324
Bsu36I CCTNAGG 1 cut(s) 606
BsuI GTATCC 1 cut(s) 116
BsuRI GGCC 2 cut(s) 468, 1108
BsuTUI ATCGAT 2 cut(s) 756, 1324
BtgI CCRYGG 3 cut(s) 142, 669, 1499
BtsCI GGATG 8 cut(s) 59, 732, 737, 784, 1194, 1317, 1395, 1509
BtsI GCAGTG 1 cut(s) 742
BtsIMutI CAGTG 5 cut(s) 264, 294, 742, 800, 1453
BtuMI TCGCGA 1 cut(s) 70
BveI ACCTGC 2 cut(s) 731, 1061
CaiI CAGNNNCTG 1 cut(s) 497
Cfr13I GGNCC 2 cut(s) 936, 1106
ClaI ATCGAT 2 cut(s) 756, 1324
Csp6I GTAC 8 cut(s) 151, 244, 328, 414, 500, 838, 943, 966
CspCI CAANNNNNGTGG 2 cut(s) 703, 738
CviAII CATG 6 cut(s) 143, 391, 670, 814, 1235, 1410
CviQI GTAC 8 cut(s) 151, 244, 328, 414, 500, 838, 943, 966
DdeI CTNAG 4 cut(s) 588, 606, 922, 1466
Eam1104I CTCTTC 1 cut(s) 1240
EarI CTCTTC 1 cut(s) 1240
Eco130I CCWWGG 5 cut(s) 142, 469, 669, 1380, 1538
Eco47I GGWCC 1 cut(s) 936
Eco57I CTGAAG 2 cut(s) 419, 761
Eco81I CCTNAGG 1 cut(s) 606
EcoNI CCTNNNNNAGG 1 cut(s) 491
EcoO109I RGGNCCY 1 cut(s) 1106
EcoT14I CCWWGG 5 cut(s) 142, 469, 669, 1380, 1538
ErhI CCWWGG 5 cut(s) 142, 469, 669, 1380, 1538
FaeI CATG 6 cut(s) 146, 394, 673, 817, 1238, 1413
FaqI GGGAC 1 cut(s) 385
FatI CATG 6 cut(s) 142, 390, 669, 813, 1234, 1409
FauI CCCGC 1 cut(s) 1489
FauNDI CATATG 1 cut(s) 1296
FbaI TGATCA 1 cut(s) 135
FblI GTMKAC 1 cut(s) 300
Fnu4HI GCNGC 7 cut(s) 186, 637, 897, 1000, 1118, 1136, 1345
FokI GGATG 8 cut(s) 46, 739, 744, 771, 1181, 1324, 1402, 1516
Fsp4HI GCNGC 7 cut(s) 186, 637, 897, 1000, 1118, 1136, 1345
FspBI CTAG 2 cut(s) 537, 1364
GluI GCNGC 7 cut(s) 186, 637, 897, 1000, 1118, 1136, 1345
GsuI CTGGAG 2 cut(s) 224, 1260
HaeIII GGCC 2 cut(s) 468, 1108
HapII CCGG 2 cut(s) 323, 465
Hin1II CATG 6 cut(s) 146, 394, 673, 817, 1238, 1413
HincII GTYRAC 2 cut(s) 337, 1548
HindII GTYRAC 2 cut(s) 337, 1548
HindIII AAGCTT 1 cut(s) 116
HinfI GANTC 3 cut(s) 362, 476, 1434
HpaI GTTAAC 1 cut(s) 337
HpaII CCGG 2 cut(s) 323, 465
HphI GGTGA 5 cut(s) 130, 485, 817, 1085, 1087
Hpy166II GTNNAC 7 cut(s) 294, 301, 328, 337, 375, 631, 1548
Hpy188I TCNGA 6 cut(s) 747, 925, 1059, 1129, 1208, 1288
Hpy188III TCNNGA 7 cut(s) 69, 133, 366, 491, 751, 1174, 1431
Hpy8I GTNNAC 7 cut(s) 294, 301, 328, 337, 375, 631, 1548
Hpy99I CGWCG 3 cut(s) 49, 52, 70
HpyAV CCTTC 6 cut(s) 441, 497, 927, 1286, 1380, 1453
HpyCH4III ACNGT 5 cut(s) 298, 399, 961, 1170, 1448
HpyCH4V TGCA 7 cut(s) 375, 636, 735, 740, 784, 800, 1443
HpyF10VI GCNNNNNNNGC 5 cut(s) 593, 999, 1076, 1114, 1300
HpyF3I CTNAG 4 cut(s) 588, 606, 922, 1466
Hsp92II CATG 6 cut(s) 146, 394, 673, 817, 1238, 1413
Ksp22I TGATCA 1 cut(s) 135
KspAI GTTAAC 1 cut(s) 337
LguI GCTCTTC 1 cut(s) 1240
LmnI GCTCC 3 cut(s) 232, 1300, 1470
Lsp1109I GCAGC 7 cut(s) 172, 648, 883, 986, 1104, 1122, 1356
LweI GCATC 4 cut(s) 244, 722, 793, 1066
MaeI CTAG 2 cut(s) 537, 1364
MaeIII GTNAC 3 cut(s) 307, 1003, 1039
MbiI CCGCTC 1 cut(s) 855
MfeI CAATTG 1 cut(s) 947
MflI RGATCY 4 cut(s) 214, 450, 461, 1054
MhlI GDGCHC 1 cut(s) 377
MluCI AATT 5 cut(s) 618, 871, 947, 1262, 1512
MmeI TCCRAC 1 cut(s) 674
MroXI GAANNNNTTC 1 cut(s) 1169
MseI TTAA 1 cut(s) 336
MslI CAYNNNNRTG 1 cut(s) 975
MspA1I CMGCKG 2 cut(s) 596, 1135
MspI CCGG 2 cut(s) 323, 465
MspR9I CCNGG 1 cut(s) 323
MunI CAATTG 1 cut(s) 947
MvnI CGCG 1 cut(s) 70
MwoI GCNNNNNNNGC 5 cut(s) 593, 999, 1076, 1114, 1300
NciI CCSGG 1 cut(s) 323
NcoI CCATGG 2 cut(s) 142, 669
NdeI CATATG 1 cut(s) 1296
NlaIII CATG 6 cut(s) 146, 394, 673, 817, 1238, 1413
NlaIV GGNNCC 5 cut(s) 452, 568, 1107, 1302, 1523
NmeAIII GCCGAG 1 cut(s) 574
NruI TCGCGA 1 cut(s) 70
OliI CACNNNNGTG 1 cut(s) 975
PaqCI CACCTGC 1 cut(s) 1061
PciSI GCTCTTC 1 cut(s) 1240
PdmI GAANNNNTTC 1 cut(s) 1169
PfeI GAWTC 3 cut(s) 362, 476, 1434
PkrI GCNGC 7 cut(s) 187, 638, 898, 1001, 1119, 1137, 1346
Ple19I CGATCG 1 cut(s) 756
PspN4I GGNNCC 5 cut(s) 452, 568, 1107, 1302, 1523
PspPI GGNCC 2 cut(s) 936, 1106
PstI CTGCAG 1 cut(s) 638
PstNI CAGNNNCTG 1 cut(s) 497
PsuI RGATCY 4 cut(s) 214, 450, 461, 1054
PvuI CGATCG 1 cut(s) 756
PvuII CAGCTG 2 cut(s) 596, 1135
RruI TCGCGA 1 cut(s) 70
RsaI GTAC 8 cut(s) 152, 245, 329, 415, 501, 839, 944, 967
RsaNI GTAC 8 cut(s) 151, 244, 328, 414, 500, 838, 943, 966
RseI CAYNNNNRTG 1 cut(s) 975
SapI GCTCTTC 1 cut(s) 1240
SaqAI TTAA 1 cut(s) 336
SatI GCNGC 7 cut(s) 186, 637, 897, 1000, 1118, 1136, 1345
Sau96I GGNCC 2 cut(s) 936, 1106
ScrFI CCNGG 1 cut(s) 323
SduI GDGCHC 1 cut(s) 377
SfaNI GCATC 4 cut(s) 244, 722, 793, 1066
SfcI CTRYAG 1 cut(s) 634
SinI GGWCC 1 cut(s) 936
SmiMI CAYNNNNRTG 1 cut(s) 975
SmlI CTYRAG 3 cut(s) 209, 455, 1339
SmoI CTYRAG 3 cut(s) 209, 455, 1339
Sse9I AATT 5 cut(s) 618, 871, 947, 1262, 1512
SsiI CCGC 2 cut(s) 853, 1482
SspMI CTAG 2 cut(s) 537, 1364
StyD4I CCNGG 1 cut(s) 321
StyI CCWWGG 5 cut(s) 142, 469, 669, 1380, 1538
TaaI ACNGT 5 cut(s) 298, 399, 961, 1170, 1448
TaqI TCGA 9 cut(s) 197, 367, 479, 694, 752, 756, 1307, 1324, 1530
TasI AATT 5 cut(s) 618, 871, 947, 1262, 1512
TatI WGTACW 2 cut(s) 243, 499
TfiI GAWTC 3 cut(s) 362, 476, 1434
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TscAI CASTG 5 cut(s) 264, 301, 742, 807, 1453
TseI GCWGC 7 cut(s) 185, 636, 896, 999, 1117, 1135, 1344
TspDTI ATGAA 9 cut(s) 45, 379, 542, 944, 1193, 1223, 1268, 1374, 1406
TspGWI ACGGA 2 cut(s) 510, 872
TspRI CASTG 5 cut(s) 264, 301, 742, 807, 1453
VneI GTGCAC 1 cut(s) 373
VpaK11BI GGWCC 1 cut(s) 936
XagI CCTNNNNNAGG 1 cut(s) 491
XapI RAATTY 1 cut(s) 618
XcmI CCANNNNNNNNNTGG 1 cut(s) 946
XmiI GTMKAC 1 cut(s) 300
XmnI GAANNNNTTC 1 cut(s) 1169
XspI CTAG 2 cut(s) 537, 1364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.